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O42933 (PMT4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dolichyl-phosphate-mannose--protein mannosyltransferase 4
EC=2.4.1.109
Gene names
Name:ogm4
Synonyms:oma4
ORF Names:SPBC16C6.09
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces ›

Protein attributes

Sequence length778 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. Required for normal cell wall and septum formation. Ref.3 Ref.4

Catalytic activity

Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.2 Ref.3 Ref.5.

Sequence similarities

Belongs to the glycosyltransferase 39 family.

Contains 3 MIR domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 778778Dolichyl-phosphate-mannose--protein mannosyltransferase 4
PRO_0000121500

Regions

Transmembrane60 – 8021Helical; Potential
Transmembrane103 – 12321Helical; Potential
Transmembrane145 – 16521Helical; Potential
Transmembrane196 – 21621Helical; Potential
Transmembrane223 – 24321Helical; Potential
Transmembrane248 – 26821Helical; Potential
Transmembrane288 – 30821Helical; Potential
Transmembrane608 – 62821Helical; Potential
Transmembrane644 – 66421Helical; Potential
Transmembrane669 – 68921Helical; Potential
Transmembrane726 – 74621Helical; Potential
Domain336 – 39661MIR 1
Domain408 – 46760MIR 2
Domain474 – 52956MIR 3

Amino acid modifications

Glycosylation401N-linked (GlcNAc...) Potential
Glycosylation3351N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O42933 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 7A0A79377D2AA8A2

FASTA77890,091
        10         20         30         40         50         60 
MASKSEKAVK KAQKLSKEPS VELTDTKSSD NVTPKQKSPN STEEDVSLNL KTLKAKKFKL 

        70         80         90        100        110        120 
AFVLITVLSF ITRFWNLNLP GEVVFDEVHF GKFASYYLQG KYFFDLHPPF AKLLLALVAK 

       130        140        150        160        170        180 
LAGYDGHYLF DNIGDNYKDN GVPYVTIRAW PALLSSLVPP VVFLIMKESG YDLLACIVSS 

       190        200        210        220        230        240 
SLVLFDNAHV TEGRLILLDA TLLFSMVCAI YCYVRFFKLR HTPFSRPWWA WLFFTGFFLS 

       250        260        270        280        290        300 
CTISTKYVGF FTFLSIGLSV CLELWYLWDI KTGLTVERFF QHFLARFFCL IFFPFLFFLF 

       310        320        330        340        350        360 
WFYMHFNILT ISGPGDSFMS LEFQETLSDN PITANSTILN YYDIVTIKHM GTNAFLHSHP 

       370        380        390        400        410        420 
EKYPIPYDDG RISSGGQQVT GYQFDDENNY WMILPADHYD PPIEAKLNVP VKNMDYIKLH 

       430        440        450        460        470        480 
HVGTNTDLMT HDVASPYHPT NEEFTTVSVD ESAGKKHEYT LFQVVMSDNT DPQRPLYTKA 

       490        500        510        520        530        540 
SSFKLIHKLT HVAMWSDPKP LPDWAFKQLE INGAKNIQTG SIFWTFDDII GLKDSRLKKE 

       550        560        570        580        590        600 
KKIPKKLPFW KKYLELQLTM FRQNNMLTEF HPYSSNPSDW FTLHHGIAFW AKSEENKQIY 

       610        620        630        640        650        660 
LLGNPIGWWI IAGTVLSTTV VAAAEILLRQ RGIRTLPETV RNHFYRSTMF FYMTYVFHYL 

       670        680        690        700        710        720 
PFFIMGRQLF LHHYLPAHLA GSLLVGAFIQ LACRKSFRSP VSAGVPIPKD VDEKGHSKCH 

       730        740        750        760        770 
RKYGHVIELI CTLLLIFVVI YCFTFFAPMT YGDKSLSVDE WTRRKWLDSW VFQYQKQN

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-105, SUBCELLULAR LOCATION.
Strain: ATCC 38364 / 968.
[3]"Characterization of O-mannosyltransferase family in Schizosaccharomyces pombe."
Tanaka N., Fujita Y., Suzuki S., Morishita M., Giga-Hama Y., Shimoda C., Takegawa K.
Biochem. Biophys. Res. Commun. 330:813-820(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]"Protein O-mannosylation is crucial for cell wall integrity, septation and viability in fission yeast."
Willer T., Brandl M., Sipiczki M., Strahl S.
Mol. Microbiol. 57:156-170(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329671 Genomic DNA. Translation: CAA16916.1.
AB027986 Genomic DNA. Translation: BAA87290.1.
PIRT39560.
RefSeqNP_596807.1. NM_001023828.2.

3D structure databases

ProteinModelPortalO42933.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-4674080.
STRING4896.SPBC16C6.09-1.

Protein family/group databases

CAZyGT39. Glycosyltransferase Family 39.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC16C6.09.1; SPBC16C6.09.1:pep; SPBC16C6.09.
GeneID2539761.
KEGGspo:SPBC16C6.09.

Organism-specific databases

PomBaseSPBC16C6.09.

Phylogenomic databases

HOGENOMHOG000157526.
KOK00728.
OMAITIKHKD.
OrthoDBEOG4ZCXCQ.

Enzyme and pathway databases

BRENDA2.4.1.109. 5615.
UniPathwayUPA00378.

Family and domain databases

InterProIPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view]
PANTHERPTHR10050. PTHR10050. 1 hit.
PfamPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view]
SMARTSM00472. MIR. 2 hits.
[Graphical view]
SUPFAMSSF82109. MIR. 1 hit.
PROSITEPS50919. MIR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20800911.

Entry information

Entry namePMT4_SCHPO
AccessionPrimary (citable) accession number: O42933
Secondary accession number(s): Q9US82
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: May 29, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents