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O42918 (AMY4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-amylase 4
EC=3.2.1.1
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Meiotic expression up-regulated protein 7
Gene names
Name:meu7
Synonyms:aah4
ORF Names:SPBC16A3.13
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length774 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactor

Binds 1 calcium ion per subunit Potential.

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor Potential Ref.3 Ref.5.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 750726Alpha-amylase 4
PRO_0000001356
Propeptide751 – 77424Removed in mature form Potential
PRO_0000255454

Sites

Active site4611Nucleophile By similarity
Metal binding1431Calcium 1 Potential
Metal binding3951Calcium 1; via carbonyl oxygen Potential
Site5531Transition state stabilizer By similarity

Amino acid modifications

Lipidation7501GPI-anchor amidated serine Potential
Glycosylation1431N-linked (GlcNAc...) Potential
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation2141N-linked (GlcNAc...) Potential
Glycosylation2431N-linked (GlcNAc...) Potential
Glycosylation2721N-linked (GlcNAc...) Potential
Glycosylation2991N-linked (GlcNAc...) Potential
Glycosylation3261N-linked (GlcNAc...) Potential
Glycosylation3551N-linked (GlcNAc...) Potential
Glycosylation3821N-linked (GlcNAc...) Potential
Glycosylation4091N-linked (GlcNAc...) Potential
Disulfide bond52 ↔ 60 By similarity
Disulfide bond229 ↔ 314 By similarity
Disulfide bond495 ↔ 539 By similarity
Disulfide bond690 ↔ 724 By similarity

Sequences

Sequence LengthMass (Da)Tools
O42918 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 92CEAAE45664269B

FASTA77489,459
        10         20         30         40         50         60 
MKLSLKDLAL SIILVLGVAP PVQALSAEEW KKQSVYNVMT DRFATSKVVP HCDVKAEKYC 

        70         80         90        100        110        120 
GGNWQGIVDH LDYIRDLGFT AISISPVVEQ LEGPEYHGEA YHGHRPKNYY RLNPHFGNEE 

       130        140        150        160        170        180 
DLKELSDALH GKGMYLMVDV AINHTISEYF EDDYFKNTYF DKTHIDHKCK EHCSCHHDKF 

       190        200        210        220        230        240 
PRPVPHNGTK PDHKPWKHEE HCHHGKFPRP VPHNGTKPDH KPWKHEEHCS CHHDKFPRPV 

       250        260        270        280        290        300 
PHNGTKPDHK PWKHEEHCSC HHDKFPRPVP HNGTKPDHKP WKHEEHCHHG KFPRPIPHNG 

       310        320        330        340        350        360 
TKPDHKPWKH EEHCHHGRFP RPVPHNGTKP DHKPWKHEEH CSCHHDKFSR PVPHNGTKPD 

       370        380        390        400        410        420 
HKPWKHEEHC HHGKFLRPVP HNVTKPDHKP WKHEEHCHHG KFPRPVPHNG TKPDHKPWKH 

       430        440        450        460        470        480 
EEHCSCHEDH SVHERPSAKG ALEDYIQTFV ETFQIDGIRF DAMGDKYRKY WPDFCKAAGV 

       490        500        510        520        530        540 
FCMGDLKSSD SLKVCDWQND LEGLSNFPVR ESAVKAFLMN NPEGIVDLAD KMTDMRGLCV 

       550        560        570        580        590        600 
NPLLLGNFVE NKDLPRMASI SSDPATLKNA IAFVLMGDGI PMMYNGQELA MRGEEVPYNR 

       610        620        630        640        650        660 
PAIWEKGFPK RNPYYKFTST INRFRKAMIE SKDGEAFLNM QSDISIVDKR ILLLRRGKVI 

       670        680        690        700        710        720 
TVLNNLGSYY IHFHYTVSAE EHKWMDVLSC KSVPVQDNRQ VFMVRNGEPM ILYPEESAYE 

       730        740        750        760        770 
MGLCPSPIQL TEDYNSEGSL SINVEEAQTS KAPEQNRGFT NVLAALLLSL LMIL

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"Comprehensive isolation of meiosis-specific genes identifies novel proteins and unusual non-coding transcripts in Schizosaccharomyces pombe."
Watanabe T., Miyashita K., Saito T.T., Yoneki T., Kakihara Y., Nabeshima K., Kishi Y.A., Shimoda C., Nojima H.
Nucleic Acids Res. 29:2327-2337(2001) [PubMed: 11376151] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 525-774.
Strain: CD16-1.
[3]"Genome-wide identification of fungal GPI proteins."
De Groot P.W., Hellingwerf K.J., Klis F.M.
Yeast 20:781-796(2003) [PubMed: 12845604] [Abstract]
Cited for: PROBABLE GPI-ANCHOR.
[4]"An alpha-amylase homologue, aah3, encodes a GPI-anchored membrane protein required for cell wall integrity and morphogenesis in Schizosaccharomyces pombe."
Morita T., Tanaka N., Hosomi A., Giga-Hama Y., Takegawa K.
Biosci. Biotechnol. Biochem. 70:1454-1463(2006) [PubMed: 16751704] [Abstract]
Cited for: GENE NAME.
[5]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329671 Genomic DNA. Translation: CAA16864.1.
AB054318 mRNA. Translation: BAB60884.1.
PIRT39539.
RefSeqNP_596776.1. NM_001023797.1.

3D structure databases

ProteinModelPortalO42918.
ModBaseSearch...

Protein-protein interaction databases

STRINGO42918.

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC16A3.13.1; SPBC16A3.13.1:pep; SPBC16A3.13.
GeneID2539749.
GenomeReviewsGene locus meu7 in contig CU329671_GR.
KEGGspo:SPBC16A3.13.
NMPDRfig|4896.1.peg.2642.

Organism-specific databases

GeneDB_SpombeSPBC16A3.13.

Phylogenomic databases

eggNOGfuNOG06463.
GeneTreeEFGT00050000000134.
OrthoDBEOG4PZNG0.

Enzyme and pathway databases

BioCycSPOM-XXX-01:SPOM-XXX-01-004759-MONOMER.

Gene expression databases

ArrayExpressO42918.

Family and domain databases

InterProIPR015340. A_amylase_DUF1966_C.
IPR015902. Alpha_amylase.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
KOK01176.
PANTHERPTHR10357. Alpha_amylase. 1 hit.
PfamPF00128. Alpha-amylase. 2 hits.
PF09260. DUF1966. 1 hit.
[Graphical view]
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAMY4_SCHPO
AccessionPrimary (citable) accession number: O42918
Secondary accession number(s): Q96WR2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: June 1, 1998
Last modified: December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents