O42908 (CYM1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial presequence protease
EC=3.4.24.-

Gene names

Name:	cym1
ORF Names:	SPBC119.17

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	882 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the peptidase M16 family. PreP subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein processing involved in protein targeting to mitochondrion Inferred from sequence orthology. Source: PomBase proteolysis involved in cellular protein catabolic process Inferred from sequence orthology. Source: PomBase
Cellular_component	mitochondrial intermembrane space Inferred from sequence orthology. Source: PomBase
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from sequence orthology. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 882	882	Mitochondrial presequence protease		PRO_0000178015
Sites
	Active site	94	1	Proton acceptor By similarity
	Metal binding	91	1	Zinc; catalytic By similarity
	Metal binding	95	1	Zinc; catalytic By similarity
	Metal binding	192	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	O42908 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 6F1DA9A346717C60	FASTA	882	98,971
	10 20 30 40 50 60 MNYAKLSIAF SKKTIKTHNC RLFQRWLHVG DKVHDFRVVD TKKVPELQLN YTRLKHEPTN 70 80 90 100 110 120 ADMIHLDRED PNSVFSIGFQ TPAENDEGIP HILEHTTLCG SNKYPVRDPF FKMLNRSLAT 130 140 150 160 170 180 FMNAFTASDF TFYPFATVNT TDYKNLRDVY LDATLFPKLR KLDFLQEGWR FEHADVNDKK 190 200 210 220 230 240 SPIIFNGVVY NEMKGQVSDS SYIFYMLFQQ HLFQGTAYGF NSGGDPLAIP DLKYEELVKF 250 260 270 280 290 300 HRSHYHPSNA KILSYGSFPL EDNLSALSET FRPFSKRELN LPNTFLKEFD QEKRVVEYGP 310 320 330 340 350 360 LDPVMAPGRQ VKTSISFLAN DTSNVYETFA LKVLSKLCFD GFSSPFYKAL IESGLGTDFA 370 380 390 400 410 420 PNSGYDSTTK RGIFSVGLEG ASEESLAKIE NLVYSIFNDL ALKGFENEKL EAILHQMEIS 430 440 450 460 470 480 LKHKSAHFGI GLAQSLPFNW FNGADPADWL SFNKQIEWLK QKNSDGKLFQ KLIKKYILEN 490 500 510 520 530 540 KSRFVFTMLP SSTFPQRLQE AEAKKLQERT SKLTDEDIAE IEKTSVKLLE AQSTPADTSC 550 560 570 580 590 600 LPTLSVSDIP ETIDETKLKF LDIAGMKAQW YDLAAGLTYI RLLLPLKNFP ESLIPYLPVY 610 620 630 640 650 660 CDACLNLGTH SESIGDLEHQ IRRYTGGISI SPSAVTNNSD VSKYELGIAI SGYALDKNVG 670 680 690 700 710 720 KLVELINKAF WNTNLSNTDK LAIMLKTSVS GITDGIAEKG HSFAKVSSAS GLTEKTSITE 730 740 750 760 770 780 QLGGLTQVKL LSQLSREESF GPLVEKLTAI REILRGTSGF KAAINASPTQ HEVVEKALQK 790 800 810 820 830 840 FMKSRGVNQQ TQTKSTSKER NGINSIKTYH ELPFQTYFAA KSCLGVPYTH PDGAPLQILS 850 860 870 880 SLLTHKYLHG EIREKGGAYG AGLSYSGIDG VLSFFTYRDS DP

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA17932.3.
PIR	T39315.
3D structure databases
ProteinModelPortal	O42908.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC119.17-1.
Protein family/group databases
MEROPS	M16.A19.
Proteomic databases
PaxDb	O42908.
PRIDE	O42908.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
PomBase	SPBC119.17.
Phylogenomic databases
eggNOG	COG1026.
HOGENOM	HOG000008829.
OrthoDB	EOG4QRMC5.
Family and domain databases
Gene3D	3.30.830.10. 2 hits.
InterPro	IPR011249. Metalloenz_LuxS/M16. IPR011237. Pept_M16_dom. IPR011765. Pept_M16_N. IPR007863. Peptidase_M16_C. IPR013578. Peptidase_M16C_assoc. [Graphical view]
Pfam	PF08367. M16C_assoc. 1 hit. PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
SUPFAM	SSF63411. Metalloenz_metal-bd. 4 hits.
PROSITE	PS00143. INSULINASE. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801247.

Entry information

Entry name

CYM1_SCHPO

Accession

Primary (citable) accession number: O42908

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program