O42898 (COMT1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable catechol O-methyltransferase 1
EC=2.1.1.6

Gene names

ORF Names:

SPBC119.03

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.2.
Sequence similarities	Belongs to the methyltransferase superfamily. Catechol-O-methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to stress Inferred from expression pattern PubMed 12529438. Source: PomBase
Cellular_component	cytosol Inferred from direct assay Ref.2. Source: PomBase nucleus Inferred from direct assay Ref.2. Source: PomBase
Molecular_function	catechol O-methyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Probable catechol O-methyltransferase 1		PRO_0000318148
Sites
	Metal binding	162	1	Magnesium By similarity
	Metal binding	190	1	Magnesium By similarity
	Metal binding	191	1	Magnesium By similarity
	Binding site	86	1	S-adenosyl-L-methionine By similarity
	Binding site	106	1	S-adenosyl-L-methionine By similarity
	Binding site	162	1	S-adenosyl-L-methionine By similarity
	Binding site	165	1	Substrate By similarity
	Binding site	191	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O42898 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 99316BCF4FAED5EA	FASTA	266	30,267
	10 20 30 40 50 60 MPHMEDNGSE KEQLFLQHIQ NLPQERLDAI RGHPELVLKE IDEFTYPDGS GVRMCIGDVK 70 80 90 100 110 120 GGFIVGKIRE RKPKIMVELG GYLGYSAILF GNEISKIPGG RYYSLEVNED YAKIAYELVK 130 140 150 160 170 180 LAGLDEIVTI MIGKACDSLV ELQQKLLHKD LGFQALDMVF IDHWKDLYVP DLRVIESLNM 190 200 210 220 230 240 IAPGTLLVAD NIITPGAPEY HKYVNMSPEE RRGYQAKVRN VNGFDFIGRW DLIYKTETKE 250 260 FEGVIRNKHR KDAVDVTECV GYAKKD

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA17918.1.
PIR	T39301.
RefSeq	NP_595284.1. NM_001021191.2.
3D structure databases
HSSP	HSSP built from PDB template 1VID based on UniProtKB P22734.
ProteinModelPortal	O42898.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC119.03-1.
Proteomic databases
PaxDb	O42898.
PRIDE	O42898.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC119.03.1; SPBC119.03.1:pep; SPBC119.03.
GeneID	2540074.
KEGG	spo:SPBC119.03.
Organism-specific databases
PomBase	SPBC119.03.
Phylogenomic databases
eggNOG	COG4122.
HOGENOM	HOG000171638.
OMA	NELVEDP.
OrthoDB	EOG41ZJM6.
Family and domain databases
InterPro	IPR025782. Catechol_O-MeTrfase. IPR002935. O-MeTrfase_3. [Graphical view]
PANTHER	PTHR10509. PTHR10509. 1 hit.
Pfam	PF01596. Methyltransf_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801211.

Entry information

Entry name

COMT1_SCHPO

Accession

Primary (citable) accession number: O42898

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program