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O42893

- TREA_SCHPO

UniProt

O42893 - TREA_SCHPO

Protein

Neutral trehalase

Gene

ntp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei283 – 2831SubstrateBy similarity
    Binding sitei327 – 3271SubstrateBy similarity
    Binding sitei336 – 3361SubstrateBy similarity
    Binding sitei455 – 4551Substrate; via carbonyl oxygenBy similarity
    Active sitei457 – 4571Proton donor/acceptorBy similarity
    Active sitei652 – 6521Proton donor/acceptorBy similarity

    GO - Molecular functioni

    1. alpha,alpha-trehalase activity Source: PomBase
    2. calcium ion binding Source: PomBase
    3. protein binding Source: PomBase

    GO - Biological processi

    1. ascospore formation Source: PomBase
    2. trehalose catabolic process Source: PomBase
    3. trehalose catabolism in response to heat stress Source: PomBase

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH37. Glycoside Hydrolase Family 37.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neutral trehalase (EC:3.2.1.28)
    Alternative name(s):
    Alpha,alpha-trehalase
    Alpha,alpha-trehalose glucohydrolase
    Gene namesi
    Name:ntp1
    ORF Names:SPBC660.07
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC660.07.

    Subcellular locationi

    Cytoplasm 2 Publications

    GO - Cellular componenti

    1. cytosol Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 735735Neutral trehalasePRO_0000173797Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei47 – 471Phosphothreonine1 Publication
    Modified residuei49 – 491Phosphoserine1 Publication
    Modified residuei50 – 501Phosphothreonine1 Publication
    Modified residuei51 – 511Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO42893.

    Interactioni

    Protein-protein interaction databases

    BioGridi277630. 34 interactions.
    MINTiMINT-4673787.
    STRINGi4896.SPBC660.07-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO42893.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni290 – 2912Substrate bindingBy similarity
    Regioni336 – 3383Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 37 family.Curated

    Phylogenomic databases

    HOGENOMiHOG000192885.
    KOiK01194.
    OMAiWPPHQIM.
    OrthoDBiEOG751NPZ.
    PhylomeDBiO42893.

    Family and domain databases

    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR001661. Glyco_hydro_37.
    IPR018232. Glyco_hydro_37_CS.
    IPR011120. Trehalase_Ca-bd.
    [Graphical view]
    PANTHERiPTHR23403. PTHR23403. 1 hit.
    PfamiPF01204. Trehalase. 1 hit.
    PF07492. Trehalase_Ca-bi. 1 hit.
    [Graphical view]
    PRINTSiPR00744. GLHYDRLASE37.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00927. TREHALASE_1. 1 hit.
    PS00928. TREHALASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O42893-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSKFSSKYV DTEAISNDDD NPFATAKSYY SKDTDLSTRV SAGRPRTLST    50
    SMEASAAPTI PELKNLRRRG SLDEHKQPRK FLVDVDKTLN ALLESEDTDR 100
    NMQITIEDTG PKVVSLGSAS SGGYRLYELR GTYQLSNLLQ ELTLAKDYGR 150
    RYILLDERRL NENPVNRLSR LIKGTFWDAL TRRIDASVLD VICRDTKDRS 200
    GSHVNRIYVP KAEQEMYEYY VRAAKERPYL NLQVEYLPEE ITPEWVRDVN 250
    DKPGLLALAM EKYQDDEGNT HLRGVPFVVP GGRFNELYGW DSYFESLGLL 300
    VDDRVDLAKG MVENFIFEIT YYGKILNANR TYYLLRSQPP FLTDMALRVY 350
    ERIKNEEGSL DFLHRAFSAT IKEYHTVWTA TPRLDPKTGL SRYRPGGLGI 400
    PPETEASHFE HLLRPYMEKY HMTLEEFTHA YNYQQIHEPA LDEYFVHDRA 450
    VRESGHDTTY RLEKVCADLA TVDLNSLLYK YETDISHVIL EYFDDKFVLP 500
    NGTIETSAIW DRRARARRAA MEKYLWSEAD SMWYDYNTKL ETKSTYESAT 550
    AFWALWAGVA TPRQAAKFVD VSLPKFEVAG GIVAGTKRSL GKVGLDNPSR 600
    QWDYPNGWSP QQILAWYGLI RYGYEEETRR LVYRWLYTIT KSFVDFNGIV 650
    VEKYDLTRPV DPHRVEAEYG NQGVNIKGVA REGFGWVNAS YEVGLTFCNS 700
    HMRRALGACT TPDVFFAGIK EESLPAFENL SIHKN 735
    Length:735
    Mass (Da):84,603
    Last modified:June 1, 1998 - v1
    Checksum:i73FF979534E91BD4
    GO

    Sequence cautioni

    The sequence BAA13934.1 differs from that shown. Reason: Frameshift at positions 556 and 643.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ224339 Genomic DNA. Translation: CAA11904.1.
    CU329671 Genomic DNA. Translation: CAA22527.1.
    AB027837 Genomic DNA. Translation: BAA87141.1.
    D89273 mRNA. Translation: BAA13934.1. Frameshift.
    PIRiT40619.
    T43203.
    RefSeqiNP_595086.1. NM_001020993.2.

    Genome annotation databases

    EnsemblFungiiSPBC660.07.1; SPBC660.07.1:pep; SPBC660.07.
    GeneIDi2541115.
    KEGGispo:SPBC660.07.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ224339 Genomic DNA. Translation: CAA11904.1 .
    CU329671 Genomic DNA. Translation: CAA22527.1 .
    AB027837 Genomic DNA. Translation: BAA87141.1 .
    D89273 mRNA. Translation: BAA13934.1 . Frameshift.
    PIRi T40619.
    T43203.
    RefSeqi NP_595086.1. NM_001020993.2.

    3D structure databases

    ProteinModelPortali O42893.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277630. 34 interactions.
    MINTi MINT-4673787.
    STRINGi 4896.SPBC660.07-1.

    Protein family/group databases

    CAZyi GH37. Glycoside Hydrolase Family 37.

    Proteomic databases

    MaxQBi O42893.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC660.07.1 ; SPBC660.07.1:pep ; SPBC660.07 .
    GeneIDi 2541115.
    KEGGi spo:SPBC660.07.

    Organism-specific databases

    PomBasei SPBC660.07.

    Phylogenomic databases

    HOGENOMi HOG000192885.
    KOi K01194.
    OMAi WPPHQIM.
    OrthoDBi EOG751NPZ.
    PhylomeDBi O42893.

    Miscellaneous databases

    NextBioi 20802228.

    Family and domain databases

    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR001661. Glyco_hydro_37.
    IPR018232. Glyco_hydro_37_CS.
    IPR011120. Trehalase_Ca-bd.
    [Graphical view ]
    PANTHERi PTHR23403. PTHR23403. 1 hit.
    Pfami PF01204. Trehalase. 1 hit.
    PF07492. Trehalase_Ca-bi. 1 hit.
    [Graphical view ]
    PRINTSi PR00744. GLHYDRLASE37.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00927. TREHALASE_1. 1 hit.
    PS00928. TREHALASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the ntp1+ gene, encoding neutral trehalase in the fission yeast Schizosaccharomyces pombe."
      Soto T., Fernandez J., Dominguez A., Vicente-Soler J., Cansado J., Gacto M.
      Biochim. Biophys. Acta 1443:225-229(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
      Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
      Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 172-359, SUBCELLULAR LOCATION.
      Strain: ATCC 38364 / 968.
    4. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
      Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
      DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 294-735.
      Strain: PR745.
    5. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-47; SER-49; THR-50 AND SER-51, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiTREA_SCHPO
    AccessioniPrimary (citable) accession number: O42893
    Secondary accession number(s): P78922, Q9UU37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3