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O42893 (TREA_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neutral trehalase

EC=3.2.1.28
Alternative name(s):
Alpha,alpha-trehalase
Alpha,alpha-trehalose glucohydrolase
Gene names
Name:ntp1
ORF Names:SPBC660.07
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length735 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose.

Subcellular location

Cytoplasm Ref.3 Ref.5.

Sequence similarities

Belongs to the glycosyl hydrolase 37 family.

Sequence caution

The sequence BAA13934.1 differs from that shown. Reason: Frameshift at positions 556 and 643.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 735735Neutral trehalase
PRO_0000173797

Regions

Region290 – 2912Substrate binding By similarity
Region336 – 3383Substrate binding By similarity

Sites

Active site4571Proton donor/acceptor By similarity
Active site6521Proton donor/acceptor By similarity
Binding site2831Substrate By similarity
Binding site3271Substrate By similarity
Binding site3361Substrate By similarity
Binding site4551Substrate; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue471Phosphothreonine Ref.6
Modified residue491Phosphoserine Ref.6
Modified residue501Phosphothreonine Ref.6
Modified residue511Phosphoserine Ref.6

Sequences

Sequence LengthMass (Da)Tools
O42893 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 73FF979534E91BD4

FASTA73584,603
        10         20         30         40         50         60 
MPSKFSSKYV DTEAISNDDD NPFATAKSYY SKDTDLSTRV SAGRPRTLST SMEASAAPTI 

        70         80         90        100        110        120 
PELKNLRRRG SLDEHKQPRK FLVDVDKTLN ALLESEDTDR NMQITIEDTG PKVVSLGSAS 

       130        140        150        160        170        180 
SGGYRLYELR GTYQLSNLLQ ELTLAKDYGR RYILLDERRL NENPVNRLSR LIKGTFWDAL 

       190        200        210        220        230        240 
TRRIDASVLD VICRDTKDRS GSHVNRIYVP KAEQEMYEYY VRAAKERPYL NLQVEYLPEE 

       250        260        270        280        290        300 
ITPEWVRDVN DKPGLLALAM EKYQDDEGNT HLRGVPFVVP GGRFNELYGW DSYFESLGLL 

       310        320        330        340        350        360 
VDDRVDLAKG MVENFIFEIT YYGKILNANR TYYLLRSQPP FLTDMALRVY ERIKNEEGSL 

       370        380        390        400        410        420 
DFLHRAFSAT IKEYHTVWTA TPRLDPKTGL SRYRPGGLGI PPETEASHFE HLLRPYMEKY 

       430        440        450        460        470        480 
HMTLEEFTHA YNYQQIHEPA LDEYFVHDRA VRESGHDTTY RLEKVCADLA TVDLNSLLYK 

       490        500        510        520        530        540 
YETDISHVIL EYFDDKFVLP NGTIETSAIW DRRARARRAA MEKYLWSEAD SMWYDYNTKL 

       550        560        570        580        590        600 
ETKSTYESAT AFWALWAGVA TPRQAAKFVD VSLPKFEVAG GIVAGTKRSL GKVGLDNPSR 

       610        620        630        640        650        660 
QWDYPNGWSP QQILAWYGLI RYGYEEETRR LVYRWLYTIT KSFVDFNGIV VEKYDLTRPV 

       670        680        690        700        710        720 
DPHRVEAEYG NQGVNIKGVA REGFGWVNAS YEVGLTFCNS HMRRALGACT TPDVFFAGIK 

       730 
EESLPAFENL SIHKN 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the ntp1+ gene, encoding neutral trehalase in the fission yeast Schizosaccharomyces pombe."
Soto T., Fernandez J., Dominguez A., Vicente-Soler J., Cansado J., Gacto M.
Biochim. Biophys. Acta 1443:225-229(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 172-359, SUBCELLULAR LOCATION.
Strain: ATCC 38364 / 968.
[4]"Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 294-735.
Strain: PR745.
[5]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-47; SER-49; THR-50 AND SER-51, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ224339 Genomic DNA. Translation: CAA11904.1.
CU329671 Genomic DNA. Translation: CAA22527.1.
AB027837 Genomic DNA. Translation: BAA87141.1.
D89273 mRNA. Translation: BAA13934.1. Frameshift.
PIRT40619.
T43203.
RefSeqNP_595086.1. NM_001020993.2.

3D structure databases

ProteinModelPortalO42893.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid277630. 34 interactions.
MINTMINT-4673787.
STRING4896.SPBC660.07-1.

Protein family/group databases

CAZyGH37. Glycoside Hydrolase Family 37.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC660.07.1; SPBC660.07.1:pep; SPBC660.07.
GeneID2541115.
KEGGspo:SPBC660.07.

Organism-specific databases

PomBaseSPBC660.07.

Phylogenomic databases

HOGENOMHOG000192885.
KOK01194.
OMAWPPHQIM.
OrthoDBEOG751NPZ.
PhylomeDBO42893.

Family and domain databases

InterProIPR008928. 6-hairpin_glycosidase-like.
IPR001661. Glyco_hydro_37.
IPR018232. Glyco_hydro_37_CS.
IPR011120. Trehalase_Ca-bd.
[Graphical view]
PANTHERPTHR23403. PTHR23403. 1 hit.
PfamPF01204. Trehalase. 1 hit.
PF07492. Trehalase_Ca-bi. 1 hit.
[Graphical view]
PRINTSPR00744. GLHYDRLASE37.
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00927. TREHALASE_1. 1 hit.
PS00928. TREHALASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20802228.

Entry information

Entry nameTREA_SCHPO
AccessionPrimary (citable) accession number: O42893
Secondary accession number(s): P78922, Q9UU37
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries