O42893 (TREA_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neutral trehalase
EC=3.2.1.28

Alternative name(s):
Alpha,alpha-trehalase
Alpha,alpha-trehalose glucohydrolase

Gene names

Name:	ntp1
ORF Names:	SPBC660.07

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose.
Subcellular location	Cytoplasm Ref.3 Ref.5.
Sequence similarities	Belongs to the glycosyl hydrolase 37 family.
Sequence caution	The sequence BAA13934.1 differs from that shown. Reason: Frameshift at positions 556 and 643.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ascospore formation Inferred from mutant phenotype PubMed 11094289. Source: PomBase cellular response to osmotic stress Inferred from direct assay PubMed 9974219. Source: PomBase cellular response to oxidative stress Inferred from direct assay PubMed 9974219. Source: PomBase response to cold Inferred from direct assay PubMed 9974219. Source: PomBase trehalose catabolism in response to heat stress Inferred from mutant phenotype PubMed 10223994. Source: PomBase
Cellular_component	cytosol Inferred from direct assay Ref.5. Source: PomBase
Molecular_function	alpha,alpha-trehalase activity Inferred from mutant phenotype PubMed 10223994PubMed 9495778. Source: PomBase calcium ion binding Inferred from direct assay PubMed 12943532. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Neutral trehalase		PRO_0000173797
Amino acid modifications
	Modified residue	47	1	Phosphothreonine Ref.6
	Modified residue	49	1	Phosphoserine Ref.6
	Modified residue	50	1	Phosphothreonine Ref.6
	Modified residue	51	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	O42893 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 73FF979534E91BD4	FASTA	735	84,603
	10 20 30 40 50 60 MPSKFSSKYV DTEAISNDDD NPFATAKSYY SKDTDLSTRV SAGRPRTLST SMEASAAPTI 70 80 90 100 110 120 PELKNLRRRG SLDEHKQPRK FLVDVDKTLN ALLESEDTDR NMQITIEDTG PKVVSLGSAS 130 140 150 160 170 180 SGGYRLYELR GTYQLSNLLQ ELTLAKDYGR RYILLDERRL NENPVNRLSR LIKGTFWDAL 190 200 210 220 230 240 TRRIDASVLD VICRDTKDRS GSHVNRIYVP KAEQEMYEYY VRAAKERPYL NLQVEYLPEE 250 260 270 280 290 300 ITPEWVRDVN DKPGLLALAM EKYQDDEGNT HLRGVPFVVP GGRFNELYGW DSYFESLGLL 310 320 330 340 350 360 VDDRVDLAKG MVENFIFEIT YYGKILNANR TYYLLRSQPP FLTDMALRVY ERIKNEEGSL 370 380 390 400 410 420 DFLHRAFSAT IKEYHTVWTA TPRLDPKTGL SRYRPGGLGI PPETEASHFE HLLRPYMEKY 430 440 450 460 470 480 HMTLEEFTHA YNYQQIHEPA LDEYFVHDRA VRESGHDTTY RLEKVCADLA TVDLNSLLYK 490 500 510 520 530 540 YETDISHVIL EYFDDKFVLP NGTIETSAIW DRRARARRAA MEKYLWSEAD SMWYDYNTKL 550 560 570 580 590 600 ETKSTYESAT AFWALWAGVA TPRQAAKFVD VSLPKFEVAG GIVAGTKRSL GKVGLDNPSR 610 620 630 640 650 660 QWDYPNGWSP QQILAWYGLI RYGYEEETRR LVYRWLYTIT KSFVDFNGIV VEKYDLTRPV 670 680 690 700 710 720 DPHRVEAEYG NQGVNIKGVA REGFGWVNAS YEVGLTFCNS HMRRALGACT TPDVFFAGIK 730 EESLPAFENL SIHKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the ntp1+ gene, encoding neutral trehalase in the fission yeast Schizosaccharomyces pombe." Soto T., Fernandez J., Dominguez A., Vicente-Soler J., Cansado J., Gacto M. Biochim. Biophys. Acta 1443:225-229(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 172-359, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.
[4]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 294-735. Strain: PR745.
[5]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-47; SER-49; THR-50 AND SER-51, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ224339 Genomic DNA. Translation: CAA11904.1. CU329671 Genomic DNA. Translation: CAA22527.1. AB027837 Genomic DNA. Translation: BAA87141.1. D89273 mRNA. Translation: BAA13934.1. Frameshift.
PIR	T40619. T43203.
RefSeq	NP_595086.1. NM_001020993.2.
3D structure databases
ProteinModelPortal	O42893.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC660.07-1.
Protein family/group databases
CAZy	GH37. Glycoside Hydrolase Family 37.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC660.07.1; SPBC660.07.1:pep; SPBC660.07.
GeneID	2541115.
KEGG	spo:SPBC660.07.
Organism-specific databases
PomBase	SPBC660.07.
Phylogenomic databases
HOGENOM	HOG000192885.
KO	K01194.
OMA	RQWDYPY.
OrthoDB	EOG4WQ49Q.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR001661. Glyco_hydro_37. IPR018232. Glyco_hydro_37_CS. IPR011120. Trehalase_Ca-bd. [Graphical view]
PANTHER	PTHR23403. PTHR23403. 1 hit.
Pfam	PF01204. Trehalase. 1 hit. PF07492. Trehalase_Ca-bi. 1 hit. [Graphical view]
PRINTS	PR00744. GLHYDRLASE37.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00927. TREHALASE_1. 1 hit. PS00928. TREHALASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20802228.

Entry information

Entry name

TREA_SCHPO

Accession

Primary (citable) accession number: O42893
Secondary accession number(s): P78922, Q9UU37

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program