O42868 (SSU72_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase II subunit A C-terminal domain phosphatase ssu72
Short name=CTD phosphatase ssu72
EC=3.1.3.16

Alternative name(s):
Suppressor of SUA7 protein 2 homolog

Gene names

Name:	ssu72
ORF Names:	SPAC3G9.04

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Processively dephosphorylates Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (rpb1) By similarity. Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Ssu72 is required for 3'-end formation of snoRNAs By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subunit structure	Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of cft1, cft2, ysh1, pta1, swd2, pfs2, dis2, yth1, ssu72, and fip1. Ref.2
Subcellular location	Cytoplasm. Nucleus Ref.3.
Sequence similarities	Belongs to the SSU72 phosphatase family.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, initiation Inferred from sequence orthology. Source: PomBase mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW termination of RNA polymerase II transcription Inferred from sequence orthology. Source: PomBase
Cellular_component	cytosol Inferred from direct assay Ref.3. Source: PomBase mRNA cleavage and polyadenylation specificity factor complex Inferred from direct assay Ref.2. Source: PomBase
Molecular_function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW phosphoric ester hydrolase activity Inferred from sequence orthology. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	RNA polymerase II subunit A C-terminal domain phosphatase ssu72		PRO_0000255612

Sequences

	Sequence		Length	Mass (Da)
	O42868 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 04BE20BEF08D76BA	FASTA	197	22,596
	10 20 30 40 50 60 MAPKTNLQIS VICASNQNRS MEAHNVLKNA GYQVDSFGTG SAVRLPGPSI DKPNIYQFGY 70 80 90 100 110 120 PYDEIYKELE AQDSRLYTAN GLLKMLDRNR RIKRAPCRWQ DQDSIYNIVI TCEERCYDAI 130 140 150 160 170 180 CEDLYRRGET LNRPVYLINV DIKDNHEEAS VGGKAILDLV NKLTEAQDKL EELFPSIMAD 190 FQSNHPKLPV LYTIHFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"A comparative analysis of an orthologous proteomic environment in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe." Roguev A., Shevchenko A., Schaft D., Thomas H., Stewart A.F., Shevchenko A. Mol. Cell. Proteomics 3:125-132(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CPF COMPLEX.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA15913.1.
PIR	T11640.
RefSeq	NP_594076.1. NM_001019509.2.
3D structure databases
ProteinModelPortal	O42868.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC3G9.04-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC3G9.04.1; SPAC3G9.04.1:pep; SPAC3G9.04.
GeneID	2543452.
KEGG	spo:SPAC3G9.04.
Organism-specific databases
PomBase	SPAC3G9.04.
Phylogenomic databases
eggNOG	COG5211.
HOGENOM	HOG000183445.
KO	K15544.
OMA	SMEAHNF.
OrthoDB	EOG4DFSXT.
Family and domain databases
InterPro	IPR006811. RNA_pol_II_suA. [Graphical view]
PANTHER	PTHR20383. PTHR20383. 1 hit.
Pfam	PF04722. Ssu72. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804465.

Entry information

Entry name

SSU72_SCHPO

Accession

Primary (citable) accession number: O42868

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program