O42824 (PGLR_PENJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Polygalacturonase Short name=PG EC=3.2.1.15 Alternative name(s): Pectinase
Organism	Penicillium janthinellum (Penicillium vitale)
Taxonomic identifier	5079 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 7 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 34	15	Potential		PRO_0000024790
	Chain	35 – 371	337	Polygalacturonase		PRO_0000024791
Sites
	Active site	210	1	Proton donor By similarity
	Active site	232	1	By similarity
Amino acid modifications
	Glycosylation	249	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O42824 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 9E3AD97A1BD2BED5	FASTA	371	38,327
	10 20 30 40 50 60 MPSYLRNLVW ATLAAGLVSA APTPSRVSDL TKKSSSTCTF SSAASASASK SSCSTIVLSN 70 80 90 100 110 120 IEVPAGKTLD LTDLKDGTKV IFEGTTTFGY KEWSGPLIKI SGSDITVEAA DGAVINADGS 130 140 150 160 170 180 RWWDGEGTNG GKTKPKFFYA HSLDDSTISG LNIKNTPVQA FSIQSDNLII DGVTIDNSDG 190 200 210 220 230 240 DENGGHNTDG FDISESTGVT IRNAVVKNQD DCIAINSGQN IYFTGGTCSG GHGLSIGSVG 250 260 270 280 290 300 GRDDNTVKNV TITDSTVTDS ANGVRIKTVY DATGSVSDVT FSDITVSGIT DYGIVIEQDY 310 320 330 340 350 360 ENGSPTGTPT SGVPITDLTV KGITGSVESD AVEVYILCGD DACSDWTWSG VDITSGQTSS 370 KCENVPSGAS C

References

[1]	"Cloning and characterization of a polygalacturonase-encoding gene from Penicillium janthinellum." Ishida Y., Kakibuchi K., Hirao Y., Izumori K. J. Ferment. Bioeng. 84:257-260(1997) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NBRC 7719.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D79980 Genomic DNA. Translation: BAA24524.1.
3D structure databases
ProteinModelPortal	O42824.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 7 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_PENJA

Accession

Primary (citable) accession number: O42824

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	May 31, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program