Reviewed, UniProtKB/Swiss-Prot O42809 (PGLR4_ASPNG)

Names and origin

Protein names

Recommended name:
    Polygalacturonase-4
    EC=3.2.1.15
Alternative name(s):
    Polygalacturonase IV
      Short name=PG-IV
    Pectinase-4

Gene names

Name:

pgaE

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 6 PbH1 repeats.
Biophysicochemical properties	pH dependence: Optimum pH is 3.8.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 39	20			PRO_0000024774
	Chain	40 – 378	339	Polygalacturonase-4		PRO_0000024775
Regions
	Repeat	103 – 125	23	PbH1 1
	Repeat	174 – 204	31	PbH1 2
	Repeat	205 – 226	22	PbH1 3
	Repeat	256 – 277	22	PbH1 4
	Repeat	285 – 307	23	PbH1 5
	Repeat	317 – 345	29	PbH1 6
Sites
	Active site	219	1	Proton donor By similarity
	Active site	241	1	By similarity
Amino acid modifications
	Glycosylation	258	1	N-linked (GlcNAc...) Potential
	Disulfide bond	43 ↔ 61		By similarity
	Disulfide bond	221 ↔ 237		By similarity
	Disulfide bond	345 ↔ 350		By similarity
	Disulfide bond	369 ↔ 378		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O42809-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 49DA6728B4BAC6E8	FASTA	378	39,626
	10 20 30 40 50 60 MVTSSSVIGL TLWAALVSAS PVADPLVTPA PKLEDLEKRA TSCTFSGSEG ASSASKSKTS 70 80 90 100 110 120 CSTIVLSDVA VPSGTTLDLT DLNDGTHVIF EGETHFGYEE WSGPLVSVSG TDITVTGADG 130 140 150 160 170 180 AYLNGDGSRW WDGEGSNGGK TKPKFFYAHD LTSSTISGIY IQNSPVQVFS IDGSTYLTME 190 200 210 220 230 240 DITIDNTDGD DGEAANTDGF DIGDSTYITI TGANVYNQDD CVAVNSGENI YFSGGVCSGG 250 260 270 280 290 300 HGLSIGSVGG RSDNTVKNVT FYDSDIKSSQ NGVRIKTIYG DTGSVSEVTY KEITLSDITD 310 320 330 340 350 360 YGIVVEQNYD DTSESPTDGI TIEDFVLDNV QGSVESSGTN IYIVCGSDSC TDWTWTDVDV 370 TGGKTSSDCE NVPDDISC

References

[1]

"pgaE encodes a fourth member of the endopolygalacturonase gene family from Aspergillus niger." Parenicova L., Benen J.A.E., Kester H.C.M., Visser J. Eur. J. Biochem. 251:72-80(1998) [PubMed: 9492270] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-59, CHARACTERIZATION. Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y14386 Genomic DNA. Translation: CAA74744.1.
3D structure databases
HSSP	HSSP built from PDB template 1IB4 based on UniProtKB O74213.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 277.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR4_ASPNG

Accession

Primary (citable) accession number: O42809

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)