Reviewed, UniProtKB/Swiss-Prot O42778 (CARP8_CANAL)

Names and origin

Protein names

Recommended name:
    Candidapepsin-8
    EC=3.4.23.24
Alternative name(s):
    Aspartate protease 8
    ACP 8
    Secreted aspartic protease 8

Gene names

Name:

SAP8

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.
Subcellular location	Secreted.
Post-translational modification	O-glycosylated By similarity.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – ?		Activation peptide Potential		PRO_0000025862
	Chain	? – 405		Candidapepsin-8		PRO_0000025863
Regions
	Compositional bias	59 – 70	12	Poly-Gln
Sites
	Active site	107	1	By similarity
	Active site	292	1	By similarity
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Disulfide bond	122 ↔ 134		By similarity
	Disulfide bond	327 ↔ 358		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O42778-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 912FC91979FE39C0	FASTA	405	43,051
	10 20 30 40 50 60 MVSIITFTKN VLVTLAFALL AQGLAIPEDI DKRAEKVVSL DFTVTRKPFN ATAHGQHHQS 70 80 90 100 110 120 QQQQQQQQQQ PAQKRGTVQT SLINEGPSYA ATITVGSNKQ QQTVIVDTGS SDLWVVDSAA 130 140 150 160 170 180 VCQVTYPGQS PTFCKQDGTY KPSSSTTSQN LGKAFSIRYE DGSSSQGTVY KDTIGLGGAS 190 200 210 220 230 240 ITNQQFADVT TTSVDQGILG IGFTGDESSP TYDNVPVTLK KQGIINKNAY SLYLNSASAS 250 260 270 280 290 300 SGTIIFGGVD NAKYTGSLTA LPITSSNELR VQLSTINIAG TTVSASTTPV LDSGTTLTYF 310 320 330 340 350 360 SQTIADKLAA AVGAKWNSYY QLYTSSCNLA GNIVFNFAKG VTISVPLSEF VLQDGNSCYF 370 380 390 400 GVSRDSATIL GDNFLRRAYA VYDLDGNTIS LAQVKYTTSS SISTL

References

[1]	"Differential regulation of SAP8 and SAP9, which encode two new members of the secreted aspartic proteinase family in Candida albicans." Monod M., Hube B., Hess D., Sanglard D. Microbiology 144:2731-2737(1998) [PubMed: 9802014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C74.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF043330 Genomic DNA. Translation: AAC69995.1.
3D structure databases
HSSP	HSSP built from PDB template 1J71 based on UniProtKB Q00663.
ModBase	Search...
Protein family/group databases
MEROPS	A01.066.
Enzyme and pathway databases
BRENDA	3.4.23.24. 1124.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
PMAP-CutDB	O42778.

Entry information

Entry name

CARP8_CANAL

Accession

Primary (citable) accession number: O42778

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)