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O42773 (PP2B1_CRYNH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit A1
EC=3.1.3.16
Alternative name(s):
Calcineurin A1
Gene names
Name:CNA1
ORF Names:CNAG_04796
OrganismCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) [Complete proteome]
Taxonomic identifier235443 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex ›

Protein attributes

Sequence length639 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin By similarity.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 1 Fe3+ ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2B subfamily.

Ontologies

Keywords
   LigandCalmodulin-binding
Iron
Metal-binding
Zinc
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoprotein phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 639639Serine/threonine-protein phosphatase 2B catalytic subunit A1
PRO_0000058832

Sites

Active site1811Proton donor By similarity
Metal binding1201Iron By similarity
Metal binding1221Iron By similarity
Metal binding1481Iron By similarity
Metal binding1481Zinc By similarity
Metal binding1801Zinc By similarity
Metal binding2291Zinc By similarity
Metal binding3111Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
O42773 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 4B92EBE361C80579

FASTA63971,500
        10         20         30         40         50         60 
MASPATQTAN AIAAINNRSN LVIPEIDFTQ HQLENGEIVS TTERVIKDVQ APAMYVPTDD 

        70         80         90        100        110        120 
QFWSKVDKTK PDIAFLKNHF YREGRLTEEQ ALYILEKGGE LLRSEPNLLE VDAPITVCGD 

       130        140        150        160        170        180 
IHGQYYDLMK LFEVGGNPAD TRYLFLGDYV DRGYFSIECV LYLWSLKMWY PDTLFLLRGN 

       190        200        210        220        230        240 
HECRHLTDYF TFKLECKHKY SETVYNACME SFCNLPLAAV MNKQFLCIHG GLSPELHTLD 

       250        260        270        280        290        300 
DLRSINRFRE PPTQGLMCDI LWADPLEDFG SEKTNENFLH NHVRGCSYFF TYNAACQFLE 

       310        320        330        340        350        360 
RNNLLSIIRA HEAQDAGYRM YRKTKTTGFP SVMTIFSAPN YLDVYSNKAA VLKYESNVMN 

       370        380        390        400        410        420 
IRQFNCTPHP YWLPNFMDVF TWSLPFVGEK ITDMLIAILN CCTKEELEEE DEEFPLNAPE 

       430        440        450        460        470        480 
PTDAESAAER RQIIKNKILA VGRMSRVFSL LREESERVSE LKSISGSNAL PAGMLASGAE 

       490        500        510        520        530        540 
GIKEAIQGFE DARKSDIENE RLPPDIIDPD EDKPASPSAS PIMPATPEEI PSEIPYDSPI 

       550        560        570        580        590        600 
TGTPRTPISS AIASGSPGSP GTPTSPSIGG PPLTAWRPGH GRRTSLGTTK TSPSTRRRSL 

       610        620        630 
ENTMHLIRDV VGGKDAQGDG QLERLAEVIS SPTKGGQGE

« Hide

References

« Hide 'large scale' references
[1]"Calcineurin is required for virulence of Cryptococcus neoformans."
Odom A., Muir S., Lim E., Toffaletti D.L., Perfect J.R., Heitman J.
EMBO J. 16:2576-2589(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
[2]"The genome sequence of Cryptococcus neoformans grubii H99."
The Broad Institute Genome Sequencing Platform
Birren B., Cuomo C., Gargeya S., Jaffe D., Young S.K., Wortman J., Zeng Q., Alvarado L., Dietrich F., Allen A., Stajich J.E., Heitman J., Kronstad J.
Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF042082 Genomic DNA. Translation: AAB97372.1.
CP003829 Genomic DNA. Translation: AFR97420.1.

3D structure databases

ProteinModelPortalO42773.
SMRO42773. Positions 39-402.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR004843. Metallo_PEstase_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP2B1_CRYNH
AccessionPrimary (citable) accession number: O42773
Secondary accession number(s): J9W089
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 1998
Last modified: April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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