Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase 2B catalytic subunit A1

Gene

CNA1

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi120 – 1201IronBy similarity
Metal bindingi122 – 1221IronBy similarity
Metal bindingi148 – 1481IronBy similarity
Metal bindingi148 – 1481ZincBy similarity
Metal bindingi180 – 1801ZincBy similarity
Active sitei181 – 1811Proton donorBy similarity
Metal bindingi229 – 2291ZincBy similarity
Metal bindingi311 – 3111ZincBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calmodulin-binding, Iron, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit A1 (EC:3.1.3.16)
Alternative name(s):
Calcineurin A1
Gene namesi
Name:CNA1
ORF Names:CNAG_04796
OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Taxonomic identifieri235443 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000010091 Componenti: Chromosome 10

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 639639Serine/threonine-protein phosphatase 2B catalytic subunit A1PRO_0000058832Add
BLAST

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Structurei

3D structure databases

ProteinModelPortaliO42773.
SMRiO42773. Positions 39-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Phylogenomic databases

OrthoDBiEOG77M8X9.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O42773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPATQTAN AIAAINNRSN LVIPEIDFTQ HQLENGEIVS TTERVIKDVQ
60 70 80 90 100
APAMYVPTDD QFWSKVDKTK PDIAFLKNHF YREGRLTEEQ ALYILEKGGE
110 120 130 140 150
LLRSEPNLLE VDAPITVCGD IHGQYYDLMK LFEVGGNPAD TRYLFLGDYV
160 170 180 190 200
DRGYFSIECV LYLWSLKMWY PDTLFLLRGN HECRHLTDYF TFKLECKHKY
210 220 230 240 250
SETVYNACME SFCNLPLAAV MNKQFLCIHG GLSPELHTLD DLRSINRFRE
260 270 280 290 300
PPTQGLMCDI LWADPLEDFG SEKTNENFLH NHVRGCSYFF TYNAACQFLE
310 320 330 340 350
RNNLLSIIRA HEAQDAGYRM YRKTKTTGFP SVMTIFSAPN YLDVYSNKAA
360 370 380 390 400
VLKYESNVMN IRQFNCTPHP YWLPNFMDVF TWSLPFVGEK ITDMLIAILN
410 420 430 440 450
CCTKEELEEE DEEFPLNAPE PTDAESAAER RQIIKNKILA VGRMSRVFSL
460 470 480 490 500
LREESERVSE LKSISGSNAL PAGMLASGAE GIKEAIQGFE DARKSDIENE
510 520 530 540 550
RLPPDIIDPD EDKPASPSAS PIMPATPEEI PSEIPYDSPI TGTPRTPISS
560 570 580 590 600
AIASGSPGSP GTPTSPSIGG PPLTAWRPGH GRRTSLGTTK TSPSTRRRSL
610 620 630
ENTMHLIRDV VGGKDAQGDG QLERLAEVIS SPTKGGQGE
Length:639
Mass (Da):71,500
Last modified:June 1, 1998 - v1
Checksum:i4B92EBE361C80579
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042082 Genomic DNA. Translation: AAB97372.1.
CP003829 Genomic DNA. Translation: AFR97420.1.
RefSeqiXP_012052213.1. XM_012196823.1.

Genome annotation databases

GeneIDi23888175.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042082 Genomic DNA. Translation: AAB97372.1.
CP003829 Genomic DNA. Translation: AFR97420.1.
RefSeqiXP_012052213.1. XM_012196823.1.

3D structure databases

ProteinModelPortaliO42773.
SMRiO42773. Positions 39-402.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23888175.

Phylogenomic databases

OrthoDBiEOG77M8X9.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Calcineurin is required for virulence of Cryptococcus neoformans."
    Odom A., Muir S., Lim E., Toffaletti D.L., Perfect J.R., Heitman J.
    EMBO J. 16:2576-2589(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
  2. "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation."
    Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V., Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F., Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A., Gaillardin C., Gerik K.J.
    , Goldberg J., Gonzalez-Hilarion S., Gujja S., Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L., Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K., Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A., Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A., Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A., Cuomo C.A., Dietrich F.S.
    PLoS Genet. 10:E1004261-E1004261(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.

Entry informationi

Entry nameiPP2B1_CRYNH
AccessioniPrimary (citable) accession number: O42773
Secondary accession number(s): J9W089
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 1998
Last modified: July 22, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.