Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

UBP2

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety in natural or engineered linear fusion proteins, irrespective of their size or the presence of an N-terminal extension to ubiquitin.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei707 – 7071NucleophilePROSITE-ProRule annotation
Active sitei1153 – 11531Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2
Gene namesi
Name:UBP2
Ordered Locus Names:KLLA0E02376g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598 Componenti: Chromosome E

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12201220Ubiquitin carboxyl-terminal hydrolase 2PRO_0000080587Add
BLAST

Proteomic databases

PRIDEiO42726.

Interactioni

Protein-protein interaction databases

STRINGi28985.O42726.

Structurei

3D structure databases

ProteinModelPortaliO42726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini698 – 1202505USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiNOG275561.
HOGENOMiHOG000094458.
InParanoidiO42726.
KOiK11849.
OMAiHRGEASY.
OrthoDBiEOG7327X3.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR025305. UCH_repeat_domain.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF13446. RPT. 2 hits.
PF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O42726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMASQEPALE LNGEQRDSVS ETAEVLRSKS LESFSDLVDD GKTLLYGDIS
60 70 80 90 100
KSFPFKTCDR ILDDIRISPW FLKKFGSSVM KQPMLQYSRE RQQLQPWNLV
110 120 130 140 150
HLIDQVNLRS RYDYDSMTCP GKNTISVMFA LLVDPNFTPN DFDDIDKFPE
160 170 180 190 200
YFFHLKITVK RRSYLENFNR HVGITHYHVL EPESLHPFDK RDIFIMEEKD
210 220 230 240 250
CRLVDQSIFV SADTNKLILV EIIKPEFNSE NLAEYRTAKI EERYKNACQE
260 270 280 290 300
FDLLNPDDIP SQAECLKTLF MIFKNPLQRK SANSEFKIIS RDSVALNSQI
310 320 330 340 350
NTDWLTTMFD FSLQKTAVED NVQSGEEYKP PDLVDYITDF KVRGIREAYT
360 370 380 390 400
RKSMEVVLIG KQSMLLENEL GTEKKTVAKC FSNQHFSASH TWWFNILNHQ
410 420 430 440 450
HIEPFPYDIN YHFINLSVAF KYIDKDIIKN YETQIALDQE NISHYFDALQ
460 470 480 490 500
YVTNAKGSYQ LIAYCGKQDV VGYEDLNNAL QVFGLDPTDI DASLLDANTM
510 520 530 540 550
IEYYNSHLLR SSDNQRKDLR NALRVLGKYL GSQKMLFLVE YEPYYNVQQA
560 570 580 590 600
YTLLKVDETV DDDIIQTAYT INIADAPGLK KDYDRAIFTI ALDRRSIFLL
610 620 630 640 650
NVLTDECPEF AQYYNCTDIS YDEALNIIEI DMNASDDVIL EVFQKKWSHG
660 670 680 690 700
IMTGPDYLLK LKMALQNIGF TRNSKLINHF LDTGIVDVSC LPVATWPAGI
710 720 730 740 750
NNVGNTCYLN SLLQFFFTIK PLRDFILNYD DDSAKLLDAS EYHSRRRIGG
760 770 780 790 800
REVSKQEELR SVQFVYHLRD LFNDMIHTNS RCVTPTKELV YLAFAPSNVE
810 820 830 840 850
VEFGDDTVAQ KELIDLTTDV VEDPTDTTRH LASCDDDIIM CQSPVALPEH
860 870 880 890 900
KTSSEAGTQQ YSVQVAKISA DQLENTLEIG RQQDVTECIG NVLAQLEIAS
910 920 930 940 950
EPLSLEDDLE QNDLVKQLFY GRIKQDLIPV NDEASVRTKY ERFLSLLVNT
960 970 980 990 1000
GDHPKDIYDA LDFYFQNDYL NLEEYGDVKR TVSISELPAV LQIQIQRVYY
1010 1020 1030 1040 1050
DREKFMPFKS IEPLPFGETL YMDRYMATED PKLLAEIQQN AELKQKLQDL
1060 1070 1080 1090 1100
KQRQRKLLSQ NEIGLTLKSS LIETKKFLQS GTLKAHDIDA DNIPSSIAYI
1110 1120 1130 1140 1150
DILINNIDEE LKSLFNKITD LETTISQQFS EFKHIGYSLF AVFIHRGEAS
1160 1170 1180 1190 1200
YGHYWVYIKD HTKNGIWRKY NDDSVTEVPQ SEVFNFTEGN TATPYFLVYV
1210 1220
REGQEQETIE PLKRILQQQE
Length:1,220
Mass (Da):141,120
Last modified:September 27, 2004 - v2
Checksum:i7362B30C368B4A47
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti646 – 6505KWSHG → EMESR in AAB94074 (PubMed:10953877).Curated
Sequence conflicti1009 – 102214KSIEP…ETLYM → QVYRAATFRRKRCTW in AAB94074 (PubMed:10953877).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022776 Genomic DNA. Translation: AAB94074.1.
CR382125 Genomic DNA. Translation: CAG99142.1.
PIRiT30529.
RefSeqiXP_454055.1. XM_454055.1.

Genome annotation databases

GeneIDi2894270.
KEGGikla:KLLA0E02377g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022776 Genomic DNA. Translation: AAB94074.1.
CR382125 Genomic DNA. Translation: CAG99142.1.
PIRiT30529.
RefSeqiXP_454055.1. XM_454055.1.

3D structure databases

ProteinModelPortaliO42726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi28985.O42726.

Protein family/group databases

MEROPSiC19.003.

Proteomic databases

PRIDEiO42726.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2894270.
KEGGikla:KLLA0E02377g.

Phylogenomic databases

eggNOGiNOG275561.
HOGENOMiHOG000094458.
InParanoidiO42726.
KOiK11849.
OMAiHRGEASY.
OrthoDBiEOG7327X3.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR025305. UCH_repeat_domain.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF13446. RPT. 2 hits.
PF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of KIUBP2, a ubiquitin hydrolase gene of Kluyveromyces lactis that can suppress a ts-mutation in CBF2, a gene encoding a centromeric protein of Saccharomyces cerevisiae."
    Winkler A.A., Korstanje R., Zonneveld B.J.M., Hooykaas P.J.J., Steensma H.Y.
    Curr. Genet. 38:17-22(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC MYA-539 / JBD100.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Entry informationi

Entry nameiUBP2_KLULA
AccessioniPrimary (citable) accession number: O42726
Secondary accession number(s): Q6CPT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 27, 2004
Last modified: January 7, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.