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Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

UBP2

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety in natural or engineered linear fusion proteins, irrespective of their size or the presence of an N-terminal extension to ubiquitin.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei707NucleophilePROSITE-ProRule annotation1
Active sitei1153Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Protein family/group databases

MEROPSiC19.A56.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2
Gene namesi
Name:UBP2
Ordered Locus Names:KLLA0E02376g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome E

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805871 – 1220Ubiquitin carboxyl-terminal hydrolase 2Add BLAST1220

Proteomic databases

PRIDEiO42726.

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_454055.1.

Structurei

3D structure databases

ProteinModelPortaliO42726.
SMRiO42726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini698 – 1202USPAdd BLAST505

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

eggNOGiKOG1863. Eukaryota.
COG5077. LUCA.
HOGENOMiHOG000094458.
InParanoidiO42726.
KOiK11849.
OMAiAVFIHRG.
OrthoDBiEOG092C04TL.

Family and domain databases

InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR025305. UCH_repeat_domain.
IPR018200. USP_CS.
IPR028889. USP_dom.
PfamiView protein in Pfam
PF13446. RPT. 3 hits.
PF00443. UCH. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.

Sequencei

Sequence statusi: Complete.

O42726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMASQEPALE LNGEQRDSVS ETAEVLRSKS LESFSDLVDD GKTLLYGDIS
60 70 80 90 100
KSFPFKTCDR ILDDIRISPW FLKKFGSSVM KQPMLQYSRE RQQLQPWNLV
110 120 130 140 150
HLIDQVNLRS RYDYDSMTCP GKNTISVMFA LLVDPNFTPN DFDDIDKFPE
160 170 180 190 200
YFFHLKITVK RRSYLENFNR HVGITHYHVL EPESLHPFDK RDIFIMEEKD
210 220 230 240 250
CRLVDQSIFV SADTNKLILV EIIKPEFNSE NLAEYRTAKI EERYKNACQE
260 270 280 290 300
FDLLNPDDIP SQAECLKTLF MIFKNPLQRK SANSEFKIIS RDSVALNSQI
310 320 330 340 350
NTDWLTTMFD FSLQKTAVED NVQSGEEYKP PDLVDYITDF KVRGIREAYT
360 370 380 390 400
RKSMEVVLIG KQSMLLENEL GTEKKTVAKC FSNQHFSASH TWWFNILNHQ
410 420 430 440 450
HIEPFPYDIN YHFINLSVAF KYIDKDIIKN YETQIALDQE NISHYFDALQ
460 470 480 490 500
YVTNAKGSYQ LIAYCGKQDV VGYEDLNNAL QVFGLDPTDI DASLLDANTM
510 520 530 540 550
IEYYNSHLLR SSDNQRKDLR NALRVLGKYL GSQKMLFLVE YEPYYNVQQA
560 570 580 590 600
YTLLKVDETV DDDIIQTAYT INIADAPGLK KDYDRAIFTI ALDRRSIFLL
610 620 630 640 650
NVLTDECPEF AQYYNCTDIS YDEALNIIEI DMNASDDVIL EVFQKKWSHG
660 670 680 690 700
IMTGPDYLLK LKMALQNIGF TRNSKLINHF LDTGIVDVSC LPVATWPAGI
710 720 730 740 750
NNVGNTCYLN SLLQFFFTIK PLRDFILNYD DDSAKLLDAS EYHSRRRIGG
760 770 780 790 800
REVSKQEELR SVQFVYHLRD LFNDMIHTNS RCVTPTKELV YLAFAPSNVE
810 820 830 840 850
VEFGDDTVAQ KELIDLTTDV VEDPTDTTRH LASCDDDIIM CQSPVALPEH
860 870 880 890 900
KTSSEAGTQQ YSVQVAKISA DQLENTLEIG RQQDVTECIG NVLAQLEIAS
910 920 930 940 950
EPLSLEDDLE QNDLVKQLFY GRIKQDLIPV NDEASVRTKY ERFLSLLVNT
960 970 980 990 1000
GDHPKDIYDA LDFYFQNDYL NLEEYGDVKR TVSISELPAV LQIQIQRVYY
1010 1020 1030 1040 1050
DREKFMPFKS IEPLPFGETL YMDRYMATED PKLLAEIQQN AELKQKLQDL
1060 1070 1080 1090 1100
KQRQRKLLSQ NEIGLTLKSS LIETKKFLQS GTLKAHDIDA DNIPSSIAYI
1110 1120 1130 1140 1150
DILINNIDEE LKSLFNKITD LETTISQQFS EFKHIGYSLF AVFIHRGEAS
1160 1170 1180 1190 1200
YGHYWVYIKD HTKNGIWRKY NDDSVTEVPQ SEVFNFTEGN TATPYFLVYV
1210 1220
REGQEQETIE PLKRILQQQE
Length:1,220
Mass (Da):141,120
Last modified:September 27, 2004 - v2
Checksum:i7362B30C368B4A47
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti646 – 650KWSHG → EMESR in AAB94074 (PubMed:10953877).Curated5
Sequence conflicti1009 – 1022KSIEP…ETLYM → QVYRAATFRRKRCTW in AAB94074 (PubMed:10953877).CuratedAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022776 Genomic DNA. Translation: AAB94074.1.
CR382125 Genomic DNA. Translation: CAG99142.1.
PIRiT30529.
RefSeqiXP_454055.1. XM_454055.1.

Genome annotation databases

EnsemblFungiiCAG99142; CAG99142; KLLA0_E02377g.
GeneIDi2894270.
KEGGikla:KLLA0E02377g.

Similar proteinsi

Entry informationi

Entry nameiUBP2_KLULA
AccessioniPrimary (citable) accession number: O42726
Secondary accession number(s): Q6CPT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 27, 2004
Last modified: October 25, 2017
This is version 106 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families