Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O42726

- UBP2_KLULA

UniProt

O42726 - UBP2_KLULA

Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

UBP2

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (27 Sep 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety in natural or engineered linear fusion proteins, irrespective of their size or the presence of an N-terminal extension to ubiquitin.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei707 – 7071NucleophilePROSITE-ProRule annotation
    Active sitei1153 – 11531Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 2
    Ubiquitin thioesterase 2
    Ubiquitin-specific-processing protease 2
    Gene namesi
    Name:UBP2
    Ordered Locus Names:KLLA0E02376g
    OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
    Taxonomic identifieri284590 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
    ProteomesiUP000000598: Chromosome E

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12201220Ubiquitin carboxyl-terminal hydrolase 2PRO_0000080587Add
    BLAST

    Proteomic databases

    PRIDEiO42726.

    Interactioni

    Protein-protein interaction databases

    STRINGi28985.O42726.

    Structurei

    3D structure databases

    ProteinModelPortaliO42726.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini698 – 1202505USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiNOG275561.
    HOGENOMiHOG000094458.
    KOiK11849.
    OMAiHRGEASY.
    OrthoDBiEOG7327X3.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR025305. UCH_repeat_domain.
    [Graphical view]
    PfamiPF13446. RPT. 2 hits.
    PF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O42726-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMASQEPALE LNGEQRDSVS ETAEVLRSKS LESFSDLVDD GKTLLYGDIS     50
    KSFPFKTCDR ILDDIRISPW FLKKFGSSVM KQPMLQYSRE RQQLQPWNLV 100
    HLIDQVNLRS RYDYDSMTCP GKNTISVMFA LLVDPNFTPN DFDDIDKFPE 150
    YFFHLKITVK RRSYLENFNR HVGITHYHVL EPESLHPFDK RDIFIMEEKD 200
    CRLVDQSIFV SADTNKLILV EIIKPEFNSE NLAEYRTAKI EERYKNACQE 250
    FDLLNPDDIP SQAECLKTLF MIFKNPLQRK SANSEFKIIS RDSVALNSQI 300
    NTDWLTTMFD FSLQKTAVED NVQSGEEYKP PDLVDYITDF KVRGIREAYT 350
    RKSMEVVLIG KQSMLLENEL GTEKKTVAKC FSNQHFSASH TWWFNILNHQ 400
    HIEPFPYDIN YHFINLSVAF KYIDKDIIKN YETQIALDQE NISHYFDALQ 450
    YVTNAKGSYQ LIAYCGKQDV VGYEDLNNAL QVFGLDPTDI DASLLDANTM 500
    IEYYNSHLLR SSDNQRKDLR NALRVLGKYL GSQKMLFLVE YEPYYNVQQA 550
    YTLLKVDETV DDDIIQTAYT INIADAPGLK KDYDRAIFTI ALDRRSIFLL 600
    NVLTDECPEF AQYYNCTDIS YDEALNIIEI DMNASDDVIL EVFQKKWSHG 650
    IMTGPDYLLK LKMALQNIGF TRNSKLINHF LDTGIVDVSC LPVATWPAGI 700
    NNVGNTCYLN SLLQFFFTIK PLRDFILNYD DDSAKLLDAS EYHSRRRIGG 750
    REVSKQEELR SVQFVYHLRD LFNDMIHTNS RCVTPTKELV YLAFAPSNVE 800
    VEFGDDTVAQ KELIDLTTDV VEDPTDTTRH LASCDDDIIM CQSPVALPEH 850
    KTSSEAGTQQ YSVQVAKISA DQLENTLEIG RQQDVTECIG NVLAQLEIAS 900
    EPLSLEDDLE QNDLVKQLFY GRIKQDLIPV NDEASVRTKY ERFLSLLVNT 950
    GDHPKDIYDA LDFYFQNDYL NLEEYGDVKR TVSISELPAV LQIQIQRVYY 1000
    DREKFMPFKS IEPLPFGETL YMDRYMATED PKLLAEIQQN AELKQKLQDL 1050
    KQRQRKLLSQ NEIGLTLKSS LIETKKFLQS GTLKAHDIDA DNIPSSIAYI 1100
    DILINNIDEE LKSLFNKITD LETTISQQFS EFKHIGYSLF AVFIHRGEAS 1150
    YGHYWVYIKD HTKNGIWRKY NDDSVTEVPQ SEVFNFTEGN TATPYFLVYV 1200
    REGQEQETIE PLKRILQQQE 1220
    Length:1,220
    Mass (Da):141,120
    Last modified:September 27, 2004 - v2
    Checksum:i7362B30C368B4A47
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti646 – 6505KWSHG → EMESR in AAB94074. (PubMed:10953877)Curated
    Sequence conflicti1009 – 102214KSIEP…ETLYM → QVYRAATFRRKRCTW in AAB94074. (PubMed:10953877)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF022776 Genomic DNA. Translation: AAB94074.1.
    CR382125 Genomic DNA. Translation: CAG99142.1.
    PIRiT30529.
    RefSeqiXP_454055.1. XM_454055.1.

    Genome annotation databases

    GeneIDi2894270.
    KEGGikla:KLLA0E02377g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF022776 Genomic DNA. Translation: AAB94074.1 .
    CR382125 Genomic DNA. Translation: CAG99142.1 .
    PIRi T30529.
    RefSeqi XP_454055.1. XM_454055.1.

    3D structure databases

    ProteinModelPortali O42726.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 28985.O42726.

    Protein family/group databases

    MEROPSi C19.003.

    Proteomic databases

    PRIDEi O42726.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2894270.
    KEGGi kla:KLLA0E02377g.

    Phylogenomic databases

    eggNOGi NOG275561.
    HOGENOMi HOG000094458.
    KOi K11849.
    OMAi HRGEASY.
    OrthoDBi EOG7327X3.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR025305. UCH_repeat_domain.
    [Graphical view ]
    Pfami PF13446. RPT. 2 hits.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of KIUBP2, a ubiquitin hydrolase gene of Kluyveromyces lactis that can suppress a ts-mutation in CBF2, a gene encoding a centromeric protein of Saccharomyces cerevisiae."
      Winkler A.A., Korstanje R., Zonneveld B.J.M., Hooykaas P.J.J., Steensma H.Y.
      Curr. Genet. 38:17-22(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC MYA-539 / JBD100.
    2. "Genome evolution in yeasts."
      Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
      , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
      Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

    Entry informationi

    Entry nameiUBP2_KLULA
    AccessioniPrimary (citable) accession number: O42726
    Secondary accession number(s): Q6CPT4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: September 27, 2004
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3