Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin-conjugating enzyme E2 6

Gene

ubc6

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the doa10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains.PROSITE-ProRule annotation

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 6 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 6
Ubiquitin carrier protein 6
Ubiquitin-protein ligase 6
Gene namesi
Name:ubc6
ORF Names:SPAC10F6.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC10F6.05c
PomBaseiSPAC10F6.05c ubc6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 206CytoplasmicSequence analysisAdd BLAST206
Transmembranei207 – 225HelicalSequence analysisAdd BLAST19

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825511 – 227Ubiquitin-conjugating enzyme E2 6Add BLAST227

Proteomic databases

MaxQBiO42646
PaxDbiO42646
PRIDEiO42646

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi278138, 49 interactors
STRINGi4896.SPAC10F6.05c.1

Structurei

3D structure databases

ProteinModelPortaliO42646
SMRiO42646
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000170329
InParanoidiO42646
KOiK04554
OMAiRNANFCE
OrthoDBiEOG092C4P07
PhylomeDBiO42646

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

O42646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKGAYKRL MKEYLALQKN PVELVDAKPA TENILEWHYI ITGPPDTPYE
60 70 80 90 100
GGQYHGTLIF PPDYPFKPPA IRMITPSGRF QTNTRLCLSF SDFHPKSWNP
110 120 130 140 150
SWMVSTILVG LVSFMTSDEI TTGGIVTSES TRRTYAKDTK RFNIMDNPKF
160 170 180 190 200
LIMFPELIDK NREDIAKAAA EAALIEPQQI HSTPVSSNEC KKNEPFNSKQ
210 220
SWVKSRWSIA VLVFFALALA RFFGADS
Length:227
Mass (Da):25,659
Last modified:June 1, 1998 - v1
Checksum:i58BE058326D843AC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22V → F in BAA13909 (PubMed:9501991).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAA15718.1
D89248 mRNA Translation: BAA13909.1
PIRiT37499
T43159
RefSeqiNP_593256.1, NM_001018653.2

Genome annotation databases

EnsemblFungiiSPAC10F6.05c.1; SPAC10F6.05c.1:pep; SPAC10F6.05c
GeneIDi2541642
KEGGispo:SPAC10F6.05c

Similar proteinsi

Entry informationi

Entry nameiUBC6_SCHPO
AccessioniPrimary (citable) accession number: O42646
Secondary accession number(s): P78897, Q1L844
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: June 1, 1998
Last modified: March 28, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health