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O42617

- PAP_CANAL

UniProt

O42617 - PAP_CANAL

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Protein
Poly(A) polymerase PAPalpha
Gene
PAPALPHA, PAP1, CaO19.10713
Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor By similarity.

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Binds 2 magnesium ions. Also active with manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Magnesium 1; catalytic By similarity
Metal bindingi99 – 991Magnesium 2; catalytic By similarity
Metal bindingi101 – 1011Magnesium 1; catalytic By similarity
Metal bindingi101 – 1011Magnesium 2; catalytic By similarity
Sitei144 – 1441Interaction with RNA By similarity
Metal bindingi153 – 1531Magnesium 2; catalytic By similarity
Binding sitei153 – 1531ATP By similarity
Binding sitei214 – 2141ATP By similarity
Binding sitei223 – 2231ATP By similarity
Sitei313 – 3131Interaction with RNA By similarity
Sitei314 – 3141Interaction with RNA By similarity
Sitei386 – 3861Interaction with RNA By similarity
Sitei391 – 3911Interaction with RNA By similarity
Sitei484 – 4841Interaction with RNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 883ATP By similarity
Nucleotide bindingi99 – 1013ATP By similarity
Nucleotide bindingi232 – 2332ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. polynucleotide adenylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. RNA polyadenylation Source: InterPro
  2. mRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase PAPalpha (EC:2.7.7.19)
Alternative name(s):
Polynucleotide adenylyltransferase alpha
Gene namesi
Name:PAPALPHA
Synonyms:PAP1
ORF Names:CaO19.10713
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0006627. PAPALPHA.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Poly(A) polymerase PAPalpha
PRO_0000051618Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO42617.
SMRiO42617. Positions 4-526.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK14376.
OrthoDBiEOG7M6DHG.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

O42617-1 [UniParc]FASTAAdd to Basket

« Hide

MNTKTYGVTE PISTNGPTPK ENILNDALIQ ELKNRGSFES EQATKKRVEV    50
LTLFQRLVQE FVYTVSKSKN MSDSMAQDAG GKVFTFGSYR LGVYGPGSDI 100
DTLVVVPKHV TRDDFFSVFA DIIRKRPELE EIACVPDAYV PIIKLEFDGI 150
SIDLIMARLN IPRVPLDLTL DDKNLLKNLD EKDLRSLNGT RVTDEILQLV 200
PKPTVFKHAL RCIKLWAQQR AVYGNIFGFP GGVAWAMLVA RICQLYPNAV 250
SSAIVEKFFN IYTKWNWPEP VLLKSIEDGP LQVRVWNPRL YPHDRLHRMP 300
VITPAYPSMC ATHNITSSTQ KVILAELSRG SSIMQEIHAG KKTWSDLFEK 350
HSFFYKYKFY LCVVAASIDS AEEHKKWSGF IESKLRQLVL KLEVAEGVEI 400
AHPYVKDFSN TFILDDKNAE DIINSYGTLS GEDFLRTLHS SDSDKDDEEF 450
KKIRLTKYYI GLDLNLTKSS DGVRKLDIQY PCAEFYSICK GSTSFTEGVN 500
FIQIKNVKLH ELSNDVYEDG EERPKKSGKK RKKVIKEDGQ KRVRNESPAS 550
SASVNGSS 558
Length:558
Mass (Da):63,268
Last modified:June 1, 1998 - v1
Checksum:i8F9B5CA8B64CC106
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF167163 Genomic DNA. Translation: AAD51412.1.
AB009394 Genomic DNA. Translation: BAA23802.1.
AACQ01000097 Genomic DNA. Translation: EAK95706.1.
RefSeqiXP_714750.1. XM_709657.1.

Genome annotation databases

GeneIDi3643608.
KEGGical:CaO19.10713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF167163 Genomic DNA. Translation: AAD51412.1 .
AB009394 Genomic DNA. Translation: BAA23802.1 .
AACQ01000097 Genomic DNA. Translation: EAK95706.1 .
RefSeqi XP_714750.1. XM_709657.1.

3D structure databases

ProteinModelPortali O42617.
SMRi O42617. Positions 4-526.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3643608.
KEGGi cal:CaO19.10713.

Organism-specific databases

CGDi CAL0006627. PAPALPHA.

Phylogenomic databases

KOi K14376.
OrthoDBi EOG7M6DHG.

Family and domain databases

Gene3Di 3.30.70.590. 1 hit.
InterProi IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view ]
PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMi SSF55003. SSF55003. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a mating type-like locus in the asexual pathogenic yeast Candida albicans."
    Hull C.M., Johnson A.D.
    Science 285:1271-1275(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  2. "Molecular cloning of Candida albicans poly(A) polymerase gene."
    Ishii N., Aoki Y., Arisawa M.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10259 / CBS 5796 / DSM 5817 / JCM 2078 / NBRC 1060.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.

Entry informationi

Entry nameiPAP_CANAL
AccessioniPrimary (citable) accession number: O42617
Secondary accession number(s): Q59YW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 1998
Last modified: November 13, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The C.albicans mating-type-like (MTL) locus contains, in addition to the genes for the regulatory proteins (MTLA1, MTLA2, MTLALPHA1 and MTLALPHA2), a and alpha idiomorphs of a phosphatidylinositol kinase (PIKA and PIKALPHA), a poly(A) polymerase (PAPA and PAPALPHA) and an oxysterol binding protein-like protein (OBPA and OBPALPHA).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi