Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O42617

- PAP_CANAL

UniProt

O42617 - PAP_CANAL

Protein

Poly(A) polymerase PAPalpha

Gene

PAPALPHA

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor By similarity.By similarity

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Binds 2 magnesium ions. Also active with manganese By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi99 – 991Magnesium 1; catalyticBy similarity
    Metal bindingi99 – 991Magnesium 2; catalyticBy similarity
    Metal bindingi101 – 1011Magnesium 1; catalyticBy similarity
    Metal bindingi101 – 1011Magnesium 2; catalyticBy similarity
    Sitei144 – 1441Interaction with RNABy similarity
    Metal bindingi153 – 1531Magnesium 2; catalyticBy similarity
    Binding sitei153 – 1531ATPBy similarity
    Binding sitei214 – 2141ATPBy similarity
    Binding sitei223 – 2231ATPBy similarity
    Sitei313 – 3131Interaction with RNABy similarity
    Sitei314 – 3141Interaction with RNABy similarity
    Sitei386 – 3861Interaction with RNABy similarity
    Sitei391 – 3911Interaction with RNABy similarity
    Sitei484 – 4841Interaction with RNABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi86 – 883ATPBy similarity
    Nucleotide bindingi99 – 1013ATPBy similarity
    Nucleotide bindingi232 – 2332ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: UniProtKB-EC
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. RNA polyadenylation Source: InterPro

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) polymerase PAPalpha (EC:2.7.7.19)
    Alternative name(s):
    Polynucleotide adenylyltransferase alpha
    Gene namesi
    Name:PAPALPHA
    Synonyms:PAP1
    ORF Names:CaO19.10713
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0006627. PAPALPHA.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 558558Poly(A) polymerase PAPalphaPRO_0000051618Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO42617.
    SMRiO42617. Positions 4-526.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the poly(A) polymerase family.Curated

    Phylogenomic databases

    KOiK14376.
    OrthoDBiEOG7M6DHG.

    Family and domain databases

    Gene3Di3.30.70.590. 1 hit.
    InterProiIPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    [Graphical view]
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
    SUPFAMiSSF55003. SSF55003. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O42617-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTKTYGVTE PISTNGPTPK ENILNDALIQ ELKNRGSFES EQATKKRVEV    50
    LTLFQRLVQE FVYTVSKSKN MSDSMAQDAG GKVFTFGSYR LGVYGPGSDI 100
    DTLVVVPKHV TRDDFFSVFA DIIRKRPELE EIACVPDAYV PIIKLEFDGI 150
    SIDLIMARLN IPRVPLDLTL DDKNLLKNLD EKDLRSLNGT RVTDEILQLV 200
    PKPTVFKHAL RCIKLWAQQR AVYGNIFGFP GGVAWAMLVA RICQLYPNAV 250
    SSAIVEKFFN IYTKWNWPEP VLLKSIEDGP LQVRVWNPRL YPHDRLHRMP 300
    VITPAYPSMC ATHNITSSTQ KVILAELSRG SSIMQEIHAG KKTWSDLFEK 350
    HSFFYKYKFY LCVVAASIDS AEEHKKWSGF IESKLRQLVL KLEVAEGVEI 400
    AHPYVKDFSN TFILDDKNAE DIINSYGTLS GEDFLRTLHS SDSDKDDEEF 450
    KKIRLTKYYI GLDLNLTKSS DGVRKLDIQY PCAEFYSICK GSTSFTEGVN 500
    FIQIKNVKLH ELSNDVYEDG EERPKKSGKK RKKVIKEDGQ KRVRNESPAS 550
    SASVNGSS 558
    Length:558
    Mass (Da):63,268
    Last modified:June 1, 1998 - v1
    Checksum:i8F9B5CA8B64CC106
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF167163 Genomic DNA. Translation: AAD51412.1.
    AB009394 Genomic DNA. Translation: BAA23802.1.
    AACQ01000097 Genomic DNA. Translation: EAK95706.1.
    RefSeqiXP_714750.1. XM_709657.1.

    Genome annotation databases

    GeneIDi3643608.
    KEGGical:CaO19.10713.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF167163 Genomic DNA. Translation: AAD51412.1 .
    AB009394 Genomic DNA. Translation: BAA23802.1 .
    AACQ01000097 Genomic DNA. Translation: EAK95706.1 .
    RefSeqi XP_714750.1. XM_709657.1.

    3D structure databases

    ProteinModelPortali O42617.
    SMRi O42617. Positions 4-526.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3643608.
    KEGGi cal:CaO19.10713.

    Organism-specific databases

    CGDi CAL0006627. PAPALPHA.

    Phylogenomic databases

    KOi K14376.
    OrthoDBi EOG7M6DHG.

    Family and domain databases

    Gene3Di 3.30.70.590. 1 hit.
    InterProi IPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    [Graphical view ]
    Pfami PF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
    SUPFAMi SSF55003. SSF55003. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a mating type-like locus in the asexual pathogenic yeast Candida albicans."
      Hull C.M., Johnson A.D.
      Science 285:1271-1275(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    2. "Molecular cloning of Candida albicans poly(A) polymerase gene."
      Ishii N., Aoki Y., Arisawa M.
      Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10259 / CBS 5796 / DSM 5817 / JCM 2078 / NBRC 1060.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.

    Entry informationi

    Entry nameiPAP_CANAL
    AccessioniPrimary (citable) accession number: O42617
    Secondary accession number(s): Q59YW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The C.albicans mating-type-like (MTL) locus contains, in addition to the genes for the regulatory proteins (MTLA1, MTLA2, MTLALPHA1 and MTLALPHA2), a and alpha idiomorphs of a phosphatidylinositol kinase (PIKA and PIKALPHA), a poly(A) polymerase (PAPA and PAPALPHA) and an oxysterol binding protein-like protein (OBPA and OBPALPHA).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3