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O42617

- PAP_CANAL

UniProt

O42617 - PAP_CANAL

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Protein

Poly(A) polymerase PAPalpha

Gene

PAPALPHA

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (By similarity).By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Magnesium 1; catalyticBy similarity
Metal bindingi99 – 991Magnesium 2; catalyticBy similarity
Metal bindingi101 – 1011Magnesium 1; catalyticBy similarity
Metal bindingi101 – 1011Magnesium 2; catalyticBy similarity
Sitei144 – 1441Interaction with RNABy similarity
Metal bindingi153 – 1531Magnesium 2; catalyticBy similarity
Binding sitei153 – 1531ATPBy similarity
Binding sitei214 – 2141ATPBy similarity
Binding sitei223 – 2231ATPBy similarity
Sitei313 – 3131Interaction with RNABy similarity
Sitei314 – 3141Interaction with RNABy similarity
Sitei386 – 3861Interaction with RNABy similarity
Sitei391 – 3911Interaction with RNABy similarity
Sitei484 – 4841Interaction with RNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 883ATPBy similarity
Nucleotide bindingi99 – 1013ATPBy similarity
Nucleotide bindingi232 – 2332ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. RNA polyadenylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase PAPalpha (EC:2.7.7.19)
Alternative name(s):
Polynucleotide adenylyltransferase alpha
Gene namesi
Name:PAPALPHA
Synonyms:PAP1
ORF Names:CaO19.10713
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0006627. PAPALPHA.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Poly(A) polymerase PAPalphaPRO_0000051618Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO42617.
SMRiO42617. Positions 4-526.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

InParanoidiO42617.
KOiK14376.
OrthoDBiEOG7M6DHG.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

O42617-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTKTYGVTE PISTNGPTPK ENILNDALIQ ELKNRGSFES EQATKKRVEV
60 70 80 90 100
LTLFQRLVQE FVYTVSKSKN MSDSMAQDAG GKVFTFGSYR LGVYGPGSDI
110 120 130 140 150
DTLVVVPKHV TRDDFFSVFA DIIRKRPELE EIACVPDAYV PIIKLEFDGI
160 170 180 190 200
SIDLIMARLN IPRVPLDLTL DDKNLLKNLD EKDLRSLNGT RVTDEILQLV
210 220 230 240 250
PKPTVFKHAL RCIKLWAQQR AVYGNIFGFP GGVAWAMLVA RICQLYPNAV
260 270 280 290 300
SSAIVEKFFN IYTKWNWPEP VLLKSIEDGP LQVRVWNPRL YPHDRLHRMP
310 320 330 340 350
VITPAYPSMC ATHNITSSTQ KVILAELSRG SSIMQEIHAG KKTWSDLFEK
360 370 380 390 400
HSFFYKYKFY LCVVAASIDS AEEHKKWSGF IESKLRQLVL KLEVAEGVEI
410 420 430 440 450
AHPYVKDFSN TFILDDKNAE DIINSYGTLS GEDFLRTLHS SDSDKDDEEF
460 470 480 490 500
KKIRLTKYYI GLDLNLTKSS DGVRKLDIQY PCAEFYSICK GSTSFTEGVN
510 520 530 540 550
FIQIKNVKLH ELSNDVYEDG EERPKKSGKK RKKVIKEDGQ KRVRNESPAS

SASVNGSS
Length:558
Mass (Da):63,268
Last modified:June 1, 1998 - v1
Checksum:i8F9B5CA8B64CC106
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167163 Genomic DNA. Translation: AAD51412.1.
AB009394 Genomic DNA. Translation: BAA23802.1.
AACQ01000097 Genomic DNA. Translation: EAK95706.1.
RefSeqiXP_714750.1. XM_709657.1.

Genome annotation databases

GeneIDi3643608.
KEGGical:CaO19.10713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167163 Genomic DNA. Translation: AAD51412.1 .
AB009394 Genomic DNA. Translation: BAA23802.1 .
AACQ01000097 Genomic DNA. Translation: EAK95706.1 .
RefSeqi XP_714750.1. XM_709657.1.

3D structure databases

ProteinModelPortali O42617.
SMRi O42617. Positions 4-526.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3643608.
KEGGi cal:CaO19.10713.

Organism-specific databases

CGDi CAL0006627. PAPALPHA.

Phylogenomic databases

InParanoidi O42617.
KOi K14376.
OrthoDBi EOG7M6DHG.

Family and domain databases

Gene3Di 3.30.70.590. 1 hit.
InterProi IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view ]
PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMi SSF55003. SSF55003. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a mating type-like locus in the asexual pathogenic yeast Candida albicans."
    Hull C.M., Johnson A.D.
    Science 285:1271-1275(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  2. "Molecular cloning of Candida albicans poly(A) polymerase gene."
    Ishii N., Aoki Y., Arisawa M.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10259 / CBS 5796 / DSM 5817 / JCM 2078 / NBRC 1060.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.

Entry informationi

Entry nameiPAP_CANAL
AccessioniPrimary (citable) accession number: O42617
Secondary accession number(s): Q59YW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The C.albicans mating-type-like (MTL) locus contains, in addition to the genes for the regulatory proteins (MTLA1, MTLA2, MTLALPHA1 and MTLALPHA2), a and alpha idiomorphs of a phosphatidylinositol kinase (PIKA and PIKALPHA), a poly(A) polymerase (PAPA and PAPALPHA) and an oxysterol binding protein-like protein (OBPA and OBPALPHA).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3