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Protein

Poly(A) polymerase PAPalpha

Gene

PAPALPHA

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (By similarity).By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Magnesium 1; catalyticBy similarity1
Metal bindingi99Magnesium 2; catalyticBy similarity1
Metal bindingi101Magnesium 1; catalyticBy similarity1
Metal bindingi101Magnesium 2; catalyticBy similarity1
Metal bindingi153Magnesium 2; catalyticBy similarity1
Binding sitei153ATPBy similarity1
Binding sitei214ATPBy similarity1
Binding sitei223ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 88ATPBy similarity3
Nucleotide bindingi99 – 101ATPBy similarity3
Nucleotide bindingi232 – 233ATPBy similarity2

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • polynucleotide adenylyltransferase activity Source: CGD
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • RNA 3'-end processing Source: InterPro
  • RNA polyadenylation Source: InterPro
  • single-species biofilm formation on inanimate substrate Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase PAPalpha (EC:2.7.7.19)
Alternative name(s):
Polynucleotide adenylyltransferase alpha
Gene namesi
Name:PAPALPHA
Synonyms:PAP1
ORF Names:CaO19.10713
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0000202028. PAPALPHA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000516181 – 558Poly(A) polymerase PAPalphaAdd BLAST558

Proteomic databases

PRIDEiO42617.

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei144Interaction with RNABy similarity1
Sitei313Interaction with RNABy similarity1
Sitei314Interaction with RNABy similarity1
Sitei386Interaction with RNABy similarity1
Sitei391Interaction with RNABy similarity1
Sitei484Interaction with RNABy similarity1

Structurei

3D structure databases

ProteinModelPortaliO42617.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

InParanoidiO42617.
KOiK14376.
OrthoDBiEOG092C1VMQ.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 2 hits.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

O42617-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTKTYGVTE PISTNGPTPK ENILNDALIQ ELKNRGSFES EQATKKRVEV
60 70 80 90 100
LTLFQRLVQE FVYTVSKSKN MSDSMAQDAG GKVFTFGSYR LGVYGPGSDI
110 120 130 140 150
DTLVVVPKHV TRDDFFSVFA DIIRKRPELE EIACVPDAYV PIIKLEFDGI
160 170 180 190 200
SIDLIMARLN IPRVPLDLTL DDKNLLKNLD EKDLRSLNGT RVTDEILQLV
210 220 230 240 250
PKPTVFKHAL RCIKLWAQQR AVYGNIFGFP GGVAWAMLVA RICQLYPNAV
260 270 280 290 300
SSAIVEKFFN IYTKWNWPEP VLLKSIEDGP LQVRVWNPRL YPHDRLHRMP
310 320 330 340 350
VITPAYPSMC ATHNITSSTQ KVILAELSRG SSIMQEIHAG KKTWSDLFEK
360 370 380 390 400
HSFFYKYKFY LCVVAASIDS AEEHKKWSGF IESKLRQLVL KLEVAEGVEI
410 420 430 440 450
AHPYVKDFSN TFILDDKNAE DIINSYGTLS GEDFLRTLHS SDSDKDDEEF
460 470 480 490 500
KKIRLTKYYI GLDLNLTKSS DGVRKLDIQY PCAEFYSICK GSTSFTEGVN
510 520 530 540 550
FIQIKNVKLH ELSNDVYEDG EERPKKSGKK RKKVIKEDGQ KRVRNESPAS

SASVNGSS
Length:558
Mass (Da):63,268
Last modified:June 1, 1998 - v1
Checksum:i8F9B5CA8B64CC106
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167163 Genomic DNA. Translation: AAD51412.1.
AB009394 Genomic DNA. Translation: BAA23802.1.
AACQ01000097 Genomic DNA. Translation: EAK95706.1.
RefSeqiXP_714750.1. XM_709657.1.

Genome annotation databases

EnsemblFungiiEAK95706; EAK95706; CaO19.10713.
GeneIDi3643608.
KEGGical:CaO19.10713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167163 Genomic DNA. Translation: AAD51412.1.
AB009394 Genomic DNA. Translation: BAA23802.1.
AACQ01000097 Genomic DNA. Translation: EAK95706.1.
RefSeqiXP_714750.1. XM_709657.1.

3D structure databases

ProteinModelPortaliO42617.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO42617.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAK95706; EAK95706; CaO19.10713.
GeneIDi3643608.
KEGGical:CaO19.10713.

Organism-specific databases

CGDiCAL0000202028. PAPALPHA.

Phylogenomic databases

InParanoidiO42617.
KOiK14376.
OrthoDBiEOG092C1VMQ.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 2 hits.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAP_CANAL
AccessioniPrimary (citable) accession number: O42617
Secondary accession number(s): Q59YW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 1998
Last modified: October 5, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The C.albicans mating-type-like (MTL) locus contains, in addition to the genes for the regulatory proteins (MTLA1, MTLA2, MTLALPHA1 and MTLALPHA2), a and alpha idiomorphs of a phosphatidylinositol kinase (PIKA and PIKALPHA), a poly(A) polymerase (PAPA and PAPALPHA) and an oxysterol binding protein-like protein (OBPA and OBPALPHA).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.