O42606 (NGN1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neurogenin-1
Short name=NGN-1

Alternative name(s):
Neurogenic differentiation factor 3
Short name=NeuroD3
Neurogenin-related protein 1

Gene names

Name:	neurog1
Synonyms:	neurod3, ngn1, ngr1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcriptional regulator. Activates transcription by binding to the E box-containing promoter By similarity. Mediates neuronal differentiation. Required for the development of Rohon-Beard spinal sensory neurons and dorsal root ganglion neurons, but not for primary motoneurons or autonomic neurons. Required for development of all cranial ganglia but not associated glial cells. Regulates epiphysial neurogenesis, acting partially redundantly with ascl1a and downstream of flh. Required for the development of basal forebrain dopaminergic neurons; involved in the specification of dopaminergic progenitor cells. May be involved in maintaining rhombomere boundaries in the hindbrain. Ref.1 Ref.2 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein By similarity.
Subcellular location	Nucleus Probable.
Tissue specificity	Embryonic nervous system. Expressed transiently in neurogenic placodes prior to delamination and formation of cranial ganglia. Expressed early (6-somite stage) in Rohon-Beard spinal sensory neurons and later in neural crest-derived dorsal root ganglion neurons. At 24 hours post-fertilization (hpf), expressed in specific regions of the brain and spinal cord. In hindbrain, expressed in presumptive neuroblasts adjacent to the rhombomere boundaries. In basal forebrain, expressed in dopaminergic progenitor cells. Ref.1 Ref.2 Ref.3 Ref.6 Ref.7 Ref.9 Ref.10
Developmental stage	First detected at the late gastrulation stage (9 hpf). Strongly expressed in distinct domains in the neural plate at the 3-somite stage. First expressed in the epiphysis at around 14 hpf. Ref.1 Ref.2 Ref.3 Ref.8
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	forebrain neuron development Inferred from mutant phenotype Ref.10. Source: UniProtKB hindbrain morphogenesis Inferred from genetic interaction Ref.9. Source: UniProtKB negative regulation of neurogenesis Inferred from mutant phenotype. Source: ZFIN neuromast development Inferred from mutant phenotype. Source: ZFIN peripheral nervous system neuron development Inferred from mutant phenotype. Source: ZFIN positive regulation of neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB posterior lateral line nerve development Inferred from mutant phenotype. Source: ZFIN transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	E-box binding Inferred from sequence or structural similarity. Source: UniProtKB chromatin binding Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from physical interaction. Source: ZFIN transcription factor binding transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Neurogenin-1		PRO_0000127399
Regions
	Domain	83 – 123	41	Helix-loop-helix motif
	DNA binding	71 – 82	12	Basic motif

Sequences

	Sequence		Length	Mass (Da)
	O42606 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: CDE333AE483DC51A	FASTA	208	22,911
	10 20 30 40 50 60 MEIVYSDMET SSCDYSFSHT DDEDSRSSLH PASPASSCGK PPASPAGLQQ KKRRRGRARN 70 80 90 100 110 120 ETTVHVVKKN RRLKANDRER NRMHNLNDAL DALRSVLPAF PDDTKLTKIE TLRFAHNYIW 130 140 150 160 170 180 ALSETIRIAD QKQGKSRDGP LLLPGLSCMA DAPSPGSDSC SWPSGASSSS SSPSYCNSDP 190 200 GSPAAMDDFG YLQTDVVYSY RNFVPSIY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The activity of neurogenin1 is controlled by local cues in the zebrafish embryo." Blader P., Fischer N., Gradwohl G., Guillemont F., Straehle U. Development 124:4557-4569(1997) [PubMed: 9409673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"Overexpression of neurogenin induces ectopic expression of HuC in zebrafish." Kim C.-H., Bae Y.-K., Yamanaka Y., Yamashita S., Shimizu T., Fujii R., Park H.-C., Yeo S.-Y., Huh T.-L., Hibi M., Hirano T. Neurosci. Lett. 239:113-116(1997) [PubMed: 9469669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Embryo.
[3]	"Expression of zebrafish bHLH genes ngn1 and nrd defines distinct stages of neural differentiation." Korzh V., Sleptsova I., Liao J., He J., Gong Z. Dev. Dyn. 213:92-104(1998) [PubMed: 9733104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Embryo.
[4]	"Danio rerio neurogenin the earliest marker of primary neurons starts to be expressed at midgastrula." Thisse B. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[6]	"Delta/Notch signaling promotes formation of zebrafish neural crest by repressing Neurogenin 1 function." Cornell R.A., Eisen J.S. Development 129:2639-2648(2002) [PubMed: 12015292] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"Neurogenin1 defines zebrafish cranial sensory ganglia precursors." Andermann P., Ungos J., Raible D.W. Dev. Biol. 251:45-58(2002) [PubMed: 12413897] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[8]	"Ash1a and neurogenin1 function downstream of floating head to regulate epiphysial neurogenesis." Cau E., Wilson S.W. Development 130:2455-2466(2003) [PubMed: 12702659] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[9]	"Wnt1 regulates neurogenesis and mediates lateral inhibition of boundary cell specification in the zebrafish hindbrain." Amoyel M., Cheng Y.-C., Jiang Y.-J., Wilkinson D.G. Development 132:775-785(2005) [PubMed: 15659486] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[10]	"Neurogenin1 is a determinant of zebrafish basal forebrain dopaminergic neurons and is regulated by the conserved zinc finger protein Tof/Fezl." Jeong J.-Y., Einhorn Z., Mercurio S., Lee S., Lau B., Mione M., Wilson S.W., Guo S. Proc. Natl. Acad. Sci. U.S.A. 103:5143-5148(2006) [PubMed: 16549779] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF017301 Genomic DNA. Translation: AAB70528.1. AF024535 mRNA. Translation: AAB82271.1. AF036149 mRNA. Translation: AAB88821.1. U94588 mRNA. Translation: AAD09254.1. BC056575 mRNA. Translation: AAH56575.1. BC066427 mRNA. Translation: AAH66427.1.
IPI	IPI00488921.
RefSeq	NP_571116.1. NM_131041.1.
UniGene	Dr.75780.
3D structure databases
ProteinModelPortal	O42606.
ModBase	Search...
Protein-protein interaction databases
STRING	O42606.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000078563; ENSDARP00000073024; ENSDARG00000056130.
GeneID	30239.
KEGG	dre:30239.
Organism-specific databases
CTD	4762.
ZFIN	ZDB-GENE-990415-174. neurog1.
Phylogenomic databases
eggNOG	fiNOG16330.
GeneTree	ENSGT00550000074162.
HOGENOM	HBG278063.
HOVERGEN	HBG101129.
InParanoid	O42606.
OMA	ARNETTV.
OrthoDB	EOG4THVVQ.
PhylomeDB	O42606.
Gene expression databases
ArrayExpress	O42606.
Bgee	O42606.
Family and domain databases
InterPro	IPR011598. HLH_DNA-bd. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
KO	K09081.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NGN1_DANRE

Accession

Primary (citable) accession number: O42606

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program