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O42579

- FZD3_XENLA

UniProt

O42579 - FZD3_XENLA

Protein

Frizzled-3

Gene

fzd3

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt8.

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB-KW
    2. Wnt-activated receptor activity Source: InterPro

    GO - Biological processi

    1. neural crest cell fate specification Source: AgBase
    2. regulation of protein localization Source: BHF-UCL
    3. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Wnt signaling pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Frizzled-3
    Short name:
    Fz-3
    Short name:
    Xfz3
    Gene namesi
    Name:fzd3
    Synonyms:fz3
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-865070. fzd3.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi501 – 5011K → M: Complete loss of activity to induce the Wnt target gene Siamois expression. 1 Publication
    Mutagenesisi502 – 5021T → V: Complete loss of activity to induce the Wnt target gene Siamois expression. 1 Publication
    Mutagenesisi503 – 5031C → S: No effect. 1 Publication
    Mutagenesisi506 – 5061W → G: Complete loss of activity to induce the Wnt target gene Siamois expression. 1 Publication
    Mutagenesisi507 – 5071A → V: No effect. 1 Publication
    Mutagenesisi508 – 5081S → A: No effect. 1 Publication
    Mutagenesisi509 – 5091F → L: No effect. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 664648Frizzled-3PRO_0000012984Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi27 ↔ 88PROSITE-ProRule annotation
    Disulfide bondi35 ↔ 81PROSITE-ProRule annotation
    Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi72 ↔ 109PROSITE-ProRule annotation
    Disulfide bondi98 ↔ 132PROSITE-ProRule annotation
    Disulfide bondi102 ↔ 126PROSITE-ProRule annotation
    Glycosylationi355 – 3551N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expression restricted to the early nervous system.

    Developmental stagei

    Low expression from cleavage stages to the end of gastrulation; increases from neurulation onward. During neurulation, first localized to the anterior neural folds. As neurulation proceeds, the expression extends to the trunk neural fold, with low levels in the posterior neural fold. At the end of neurulation, strongly expressed as a large band in the midbrain. After neural tube closure, also detected in the optic lobes and otic vesicles. During the late development, expression remains restricted to the nervous system. Detected until at least the larval stages.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-216470.

    Structurei

    3D structure databases

    ProteinModelPortaliO42579.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini17 – 204188ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini226 – 23611CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini258 – 28730ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini309 – 32719CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini349 – 37325ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini395 – 41925CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini441 – 47636ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini498 – 664167CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei205 – 22521Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei237 – 25721Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei288 – 30821Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei328 – 34821Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei374 – 39421Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei420 – 44021Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei477 – 49721Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 135114FZPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi501 – 5066Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family membersBy similarity

    Domaini

    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.1 Publication
    The FZ domain is involved in binding with Wnt ligands.By similarity

    Sequence similaritiesi

    Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG006977.
    KOiK02329.

    Family and domain databases

    Gene3Di1.10.2000.10. 1 hit.
    InterProiIPR000539. Frizzled.
    IPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR026553. FZD3_vertebrates.
    IPR017981. GPCR_2-like.
    [Graphical view]
    PANTHERiPTHR11309. PTHR11309. 1 hit.
    PTHR11309:SF22. PTHR11309:SF22. 1 hit.
    PfamiPF01534. Frizzled. 1 hit.
    PF01392. Fz. 1 hit.
    [Graphical view]
    PRINTSiPR00489. FRIZZLED.
    SMARTiSM00063. FRI. 1 hit.
    [Graphical view]
    SUPFAMiSSF63501. SSF63501. 1 hit.
    PROSITEiPS50038. FZ. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O42579-1 [UniParc]FASTAAdd to Basket

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    MAAYLISFIW VSVILAQKSM GHSLFACEPI TLRMCQDLPY NSTFMPNLLN    50
    HYDQQTAALA MEPFHPMVNL ECSRDLRPFL CALYTPVCME YGRMTLPCRK 100
    LCQRAYNECF KLMEMFGVPW PEEMECSRFP DCDEPYPRIV DISLSGEPSE 150
    ETPLAVQRDY GFWCPRELKI DPDLRSSFLG VRDCSPPCPH MYFRREELSF 200
    ARYFIGVISI VCLSATLFTF LTFLIDVTRF RYPERPIIFY AVCYMMVSLI 250
    FFIGFLLEDK VACNGANPSQ YKASTVTQGS HNKACTMLFM VLYFFTMAGS 300
    VWWVILTITW FLAAVPKWGS EAIEKKALLF HASAWGIPGT LTIILLAMNK 350
    IEGDNISGVC FVGLYDVHAL RYFVLAPLCL DVVVGVSLLL AGIISLNRVR 400
    IEIPLEKENQ DKLVKFMIRI GVFSILYLVP LLVVIGCYFY EQAYRGVWET 450
    TWVQERCREY HIPCPYKVTQ TSRPDLILFL MKYLMLLVVG IPSVFWVGSK 500
    KTCFEWASFF HGRKKKAGVN ESRQVLQEPD FAQSLLRDPN TPIVRKSRGT 550
    STQGTSTHAS STQLAMLDDQ RSKAGSVQSK VSSYHGSLHR SRDGRYTPCS 600
    YRGIEERLPH GSMSHLTDHS RHSSTHRLNE QSHQGSIRDL SNPLAHISHG 650
    TSMNRVIEAD ATSA 664
    Length:664
    Mass (Da):75,619
    Last modified:January 1, 1998 - v1
    Checksum:i51BCC43BDA1791E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001754 mRNA. Translation: CAA04977.1.
    RefSeqiNP_001083918.1. NM_001090449.1.
    UniGeneiXl.42572.

    Genome annotation databases

    GeneIDi399190.
    KEGGixla:399190.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001754 mRNA. Translation: CAA04977.1 .
    RefSeqi NP_001083918.1. NM_001090449.1.
    UniGenei Xl.42572.

    3D structure databases

    ProteinModelPortali O42579.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-216470.

    Protein family/group databases

    GPCRDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 399190.
    KEGGi xla:399190.

    Organism-specific databases

    CTDi 7976.
    Xenbasei XB-GENE-865070. fzd3.

    Phylogenomic databases

    HOVERGENi HBG006977.
    KOi K02329.

    Family and domain databases

    Gene3Di 1.10.2000.10. 1 hit.
    InterProi IPR000539. Frizzled.
    IPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR026553. FZD3_vertebrates.
    IPR017981. GPCR_2-like.
    [Graphical view ]
    PANTHERi PTHR11309. PTHR11309. 1 hit.
    PTHR11309:SF22. PTHR11309:SF22. 1 hit.
    Pfami PF01534. Frizzled. 1 hit.
    PF01392. Fz. 1 hit.
    [Graphical view ]
    PRINTSi PR00489. FRIZZLED.
    SMARTi SM00063. FRI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63501. SSF63501. 1 hit.
    PROSITEi PS50038. FZ. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of Xfz3, a Xenopus frizzled family member, is restricted to the early nervous system."
      Shi D.-L., Goisset C., Boucaut J.-C.
      Mech. Dev. 70:35-47(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Embryo.
    2. "The C-terminal cytoplasmic Lys-Thr-X-X-X-Trp motif in frizzled receptors mediates Wnt/beta-catenin signalling."
      Umbhauer M., Djiane A., Goisset C., Penzo-Mendez A., Riou J.-F., Boucaut J.-C., Shi D.-L.
      EMBO J. 19:4944-4954(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS, DOMAIN K-T-X-X-X-W MOTIF, COUPLING TO BETA-CATENIN PATHWAY.

    Entry informationi

    Entry nameiFZD3_XENLA
    AccessioniPrimary (citable) accession number: O42579
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 5, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3