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Protein

Frizzled-3

Gene

fzd3

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activated by Wnt8. Involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Plays a role in controlling early axon growth and guidance processes necessary for the formation of a subset of central and peripheral major fiber tracts. Involved in the migration of cranial neural crest cells. May also be implicated in the transmission of sensory information from the trunk and limbs to the brain. Controls commissural sensory axons guidance after midline crossing along the anterior-posterior axis in the developing spinal cord in a Wnt-dependent signaling pathway. Together with FZD6, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle in a beta-catenin-dependent manner (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • nervous system development Source: UniProtKB-KW
  • neural crest cell fate specification Source: AgBase
  • regulation of protein localization Source: BHF-UCL
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Neurogenesis, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Frizzled-3
Short name:
Fz-3
Short name:
Xfz3
Gene namesi
Name:fzd3
Synonyms:fz3
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865070. fzd3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 204188ExtracellularSequence AnalysisAdd
BLAST
Transmembranei205 – 22521Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini226 – 23611CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei237 – 25721Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini258 – 28730ExtracellularSequence AnalysisAdd
BLAST
Transmembranei288 – 30821Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini309 – 32719CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei328 – 34821Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini349 – 37325ExtracellularSequence AnalysisAdd
BLAST
Transmembranei374 – 39421Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini395 – 41925CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei420 – 44021Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini441 – 47636ExtracellularSequence AnalysisAdd
BLAST
Transmembranei477 – 49721Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini498 – 664167CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi501 – 5011K → M: Complete loss of activity to induce the Wnt target gene Siamois expression. 1 Publication
Mutagenesisi502 – 5021T → V: Complete loss of activity to induce the Wnt target gene Siamois expression. 1 Publication
Mutagenesisi503 – 5031C → S: No effect. 1 Publication
Mutagenesisi506 – 5061W → G: Complete loss of activity to induce the Wnt target gene Siamois expression. 1 Publication
Mutagenesisi507 – 5071A → V: No effect. 1 Publication
Mutagenesisi508 – 5081S → A: No effect. 1 Publication
Mutagenesisi509 – 5091F → L: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 664648Frizzled-3PRO_0000012984Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 88PROSITE-ProRule annotation
Disulfide bondi35 ↔ 81PROSITE-ProRule annotation
Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi72 ↔ 109PROSITE-ProRule annotation
Disulfide bondi98 ↔ 132PROSITE-ProRule annotation
Disulfide bondi102 ↔ 126PROSITE-ProRule annotation
Glycosylationi355 – 3551N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expression restricted to the early nervous system.

Developmental stagei

Low expression from cleavage stages to the end of gastrulation; increases from neurulation onward. During neurulation, first localized to the anterior neural folds. As neurulation proceeds, the expression extends to the trunk neural fold, with low levels in the posterior neural fold. At the end of neurulation, strongly expressed as a large band in the midbrain. After neural tube closure, also detected in the optic lobes and otic vesicles. During the late development, expression remains restricted to the nervous system. Detected until at least the larval stages.

Interactioni

Protein-protein interaction databases

MINTiMINT-216470.

Structurei

3D structure databases

ProteinModelPortaliO42579.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 135114FZPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi501 – 5066Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family membersBy similarity

Domaini

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.1 Publication
The FZ domain is involved in binding with Wnt ligands.By similarity

Sequence similaritiesi

Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG006977.
KOiK02329.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026553. FZD3_vertebrates.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF22. PTHR11309:SF22. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O42579-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAYLISFIW VSVILAQKSM GHSLFACEPI TLRMCQDLPY NSTFMPNLLN
60 70 80 90 100
HYDQQTAALA MEPFHPMVNL ECSRDLRPFL CALYTPVCME YGRMTLPCRK
110 120 130 140 150
LCQRAYNECF KLMEMFGVPW PEEMECSRFP DCDEPYPRIV DISLSGEPSE
160 170 180 190 200
ETPLAVQRDY GFWCPRELKI DPDLRSSFLG VRDCSPPCPH MYFRREELSF
210 220 230 240 250
ARYFIGVISI VCLSATLFTF LTFLIDVTRF RYPERPIIFY AVCYMMVSLI
260 270 280 290 300
FFIGFLLEDK VACNGANPSQ YKASTVTQGS HNKACTMLFM VLYFFTMAGS
310 320 330 340 350
VWWVILTITW FLAAVPKWGS EAIEKKALLF HASAWGIPGT LTIILLAMNK
360 370 380 390 400
IEGDNISGVC FVGLYDVHAL RYFVLAPLCL DVVVGVSLLL AGIISLNRVR
410 420 430 440 450
IEIPLEKENQ DKLVKFMIRI GVFSILYLVP LLVVIGCYFY EQAYRGVWET
460 470 480 490 500
TWVQERCREY HIPCPYKVTQ TSRPDLILFL MKYLMLLVVG IPSVFWVGSK
510 520 530 540 550
KTCFEWASFF HGRKKKAGVN ESRQVLQEPD FAQSLLRDPN TPIVRKSRGT
560 570 580 590 600
STQGTSTHAS STQLAMLDDQ RSKAGSVQSK VSSYHGSLHR SRDGRYTPCS
610 620 630 640 650
YRGIEERLPH GSMSHLTDHS RHSSTHRLNE QSHQGSIRDL SNPLAHISHG
660
TSMNRVIEAD ATSA
Length:664
Mass (Da):75,619
Last modified:January 1, 1998 - v1
Checksum:i51BCC43BDA1791E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001754 mRNA. Translation: CAA04977.1.
RefSeqiNP_001083918.1. NM_001090449.1.
UniGeneiXl.42572.

Genome annotation databases

GeneIDi399190.
KEGGixla:399190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001754 mRNA. Translation: CAA04977.1.
RefSeqiNP_001083918.1. NM_001090449.1.
UniGeneiXl.42572.

3D structure databases

ProteinModelPortaliO42579.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-216470.

Protein family/group databases

GPCRDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399190.
KEGGixla:399190.

Organism-specific databases

CTDi7976.
XenbaseiXB-GENE-865070. fzd3.

Phylogenomic databases

HOVERGENiHBG006977.
KOiK02329.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026553. FZD3_vertebrates.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF22. PTHR11309:SF22. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of Xfz3, a Xenopus frizzled family member, is restricted to the early nervous system."
    Shi D.-L., Goisset C., Boucaut J.-C.
    Mech. Dev. 70:35-47(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "The C-terminal cytoplasmic Lys-Thr-X-X-X-Trp motif in frizzled receptors mediates Wnt/beta-catenin signalling."
    Umbhauer M., Djiane A., Goisset C., Penzo-Mendez A., Riou J.-F., Boucaut J.-C., Shi D.-L.
    EMBO J. 19:4944-4954(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS, DOMAIN K-T-X-X-X-W MOTIF, COUPLING TO BETA-CATENIN PATHWAY.

Entry informationi

Entry nameiFZD3_XENLA
AccessioniPrimary (citable) accession number: O42579
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: January 1, 1998
Last modified: March 4, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.