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O42579

- FZD3_XENLA

UniProt

O42579 - FZD3_XENLA

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Protein
Frizzled-3
Gene
fzd3, fz3
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt8.

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB-KW
  2. Wnt-activated receptor activity Source: InterPro

GO - Biological processi

  1. Wnt signaling pathway Source: UniProtKB-KW
  2. neural crest cell fate specification Source: AgBase
  3. regulation of protein localization Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Frizzled-3
Short name:
Fz-3
Short name:
Xfz3
Gene namesi
Name:fzd3
Synonyms:fz3
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865070. fzd3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 204188Extracellular Reviewed prediction
Add
BLAST
Transmembranei205 – 22521Helical; Name=1; Reviewed prediction
Add
BLAST
Topological domaini226 – 23611Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei237 – 25721Helical; Name=2; Reviewed prediction
Add
BLAST
Topological domaini258 – 28730Extracellular Reviewed prediction
Add
BLAST
Transmembranei288 – 30821Helical; Name=3; Reviewed prediction
Add
BLAST
Topological domaini309 – 32719Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei328 – 34821Helical; Name=4; Reviewed prediction
Add
BLAST
Topological domaini349 – 37325Extracellular Reviewed prediction
Add
BLAST
Transmembranei374 – 39421Helical; Name=5; Reviewed prediction
Add
BLAST
Topological domaini395 – 41925Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei420 – 44021Helical; Name=6; Reviewed prediction
Add
BLAST
Topological domaini441 – 47636Extracellular Reviewed prediction
Add
BLAST
Transmembranei477 – 49721Helical; Name=7; Reviewed prediction
Add
BLAST
Topological domaini498 – 664167Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi501 – 5011K → M: Complete loss of activity to induce the Wnt target gene Siamois expression.
Mutagenesisi502 – 5021T → V: Complete loss of activity to induce the Wnt target gene Siamois expression.
Mutagenesisi503 – 5031C → S: No effect.
Mutagenesisi506 – 5061W → G: Complete loss of activity to induce the Wnt target gene Siamois expression.
Mutagenesisi507 – 5071A → V: No effect.
Mutagenesisi508 – 5081S → A: No effect.
Mutagenesisi509 – 5091F → L: No effect.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616 Reviewed prediction
Add
BLAST
Chaini17 – 664648Frizzled-3
PRO_0000012984Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 88 By similarity
Disulfide bondi35 ↔ 81 By similarity
Glycosylationi41 – 411N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi72 ↔ 109 By similarity
Disulfide bondi98 ↔ 132 By similarity
Disulfide bondi102 ↔ 126 By similarity
Glycosylationi355 – 3551N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expression restricted to the early nervous system.

Developmental stagei

Low expression from cleavage stages to the end of gastrulation; increases from neurulation onward. During neurulation, first localized to the anterior neural folds. As neurulation proceeds, the expression extends to the trunk neural fold, with low levels in the posterior neural fold. At the end of neurulation, strongly expressed as a large band in the midbrain. After neural tube closure, also detected in the optic lobes and otic vesicles. During the late development, expression remains restricted to the nervous system. Detected until at least the larval stages.

Interactioni

Protein-protein interaction databases

MINTiMINT-216470.

Structurei

3D structure databases

ProteinModelPortaliO42579.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 135114FZ
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi501 – 5066Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members By similarity

Domaini

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.1 Publication
The FZ domain is involved in binding with Wnt ligands By similarity.1 Publication

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG006977.
KOiK02329.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026553. FZD3_vertebrates.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF22. PTHR11309:SF22. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O42579-1 [UniParc]FASTAAdd to Basket

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MAAYLISFIW VSVILAQKSM GHSLFACEPI TLRMCQDLPY NSTFMPNLLN    50
HYDQQTAALA MEPFHPMVNL ECSRDLRPFL CALYTPVCME YGRMTLPCRK 100
LCQRAYNECF KLMEMFGVPW PEEMECSRFP DCDEPYPRIV DISLSGEPSE 150
ETPLAVQRDY GFWCPRELKI DPDLRSSFLG VRDCSPPCPH MYFRREELSF 200
ARYFIGVISI VCLSATLFTF LTFLIDVTRF RYPERPIIFY AVCYMMVSLI 250
FFIGFLLEDK VACNGANPSQ YKASTVTQGS HNKACTMLFM VLYFFTMAGS 300
VWWVILTITW FLAAVPKWGS EAIEKKALLF HASAWGIPGT LTIILLAMNK 350
IEGDNISGVC FVGLYDVHAL RYFVLAPLCL DVVVGVSLLL AGIISLNRVR 400
IEIPLEKENQ DKLVKFMIRI GVFSILYLVP LLVVIGCYFY EQAYRGVWET 450
TWVQERCREY HIPCPYKVTQ TSRPDLILFL MKYLMLLVVG IPSVFWVGSK 500
KTCFEWASFF HGRKKKAGVN ESRQVLQEPD FAQSLLRDPN TPIVRKSRGT 550
STQGTSTHAS STQLAMLDDQ RSKAGSVQSK VSSYHGSLHR SRDGRYTPCS 600
YRGIEERLPH GSMSHLTDHS RHSSTHRLNE QSHQGSIRDL SNPLAHISHG 650
TSMNRVIEAD ATSA 664
Length:664
Mass (Da):75,619
Last modified:January 1, 1998 - v1
Checksum:i51BCC43BDA1791E5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001754 mRNA. Translation: CAA04977.1.
RefSeqiNP_001083918.1. NM_001090449.1.
UniGeneiXl.42572.

Genome annotation databases

GeneIDi399190.
KEGGixla:399190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001754 mRNA. Translation: CAA04977.1 .
RefSeqi NP_001083918.1. NM_001090449.1.
UniGenei Xl.42572.

3D structure databases

ProteinModelPortali O42579.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-216470.

Protein family/group databases

GPCRDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 399190.
KEGGi xla:399190.

Organism-specific databases

CTDi 7976.
Xenbasei XB-GENE-865070. fzd3.

Phylogenomic databases

HOVERGENi HBG006977.
KOi K02329.

Family and domain databases

Gene3Di 1.10.2000.10. 1 hit.
InterProi IPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026553. FZD3_vertebrates.
IPR017981. GPCR_2-like.
[Graphical view ]
PANTHERi PTHR11309. PTHR11309. 1 hit.
PTHR11309:SF22. PTHR11309:SF22. 1 hit.
Pfami PF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view ]
PRINTSi PR00489. FRIZZLED.
SMARTi SM00063. FRI. 1 hit.
[Graphical view ]
SUPFAMi SSF63501. SSF63501. 1 hit.
PROSITEi PS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Expression of Xfz3, a Xenopus frizzled family member, is restricted to the early nervous system."
    Shi D.-L., Goisset C., Boucaut J.-C.
    Mech. Dev. 70:35-47(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "The C-terminal cytoplasmic Lys-Thr-X-X-X-Trp motif in frizzled receptors mediates Wnt/beta-catenin signalling."
    Umbhauer M., Djiane A., Goisset C., Penzo-Mendez A., Riou J.-F., Boucaut J.-C., Shi D.-L.
    EMBO J. 19:4944-4954(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS, DOMAIN K-T-X-X-X-W MOTIF, COUPLING TO BETA-CATENIN PATHWAY.

Entry informationi

Entry nameiFZD3_XENLA
AccessioniPrimary (citable) accession number: O42579
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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