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O42579 (FZD3_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Frizzled-3

Short name=Fz-3
Short name=Xfz3
Gene names
Name:fzd3
Synonyms:fz3
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length664 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt8.

Subcellular location

Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Probable.

Tissue specificity

Expression restricted to the early nervous system.

Developmental stage

Low expression from cleavage stages to the end of gastrulation; increases from neurulation onward. During neurulation, first localized to the anterior neural folds. As neurulation proceeds, the expression extends to the trunk neural fold, with low levels in the posterior neural fold. At the end of neurulation, strongly expressed as a large band in the midbrain. After neural tube closure, also detected in the optic lobes and otic vesicles. During the late development, expression remains restricted to the nervous system. Detected until at least the larval stages.

Domain

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway. Ref.2

The FZ domain is involved in binding with Wnt ligands By similarity. Ref.2

Sequence similarities

Belongs to the G-protein coupled receptor Fz/Smo family.

Contains 1 FZ (frizzled) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 664648Frizzled-3
PRO_0000012984

Regions

Topological domain17 – 204188Extracellular Potential
Transmembrane205 – 22521Helical; Name=1; Potential
Topological domain226 – 23611Cytoplasmic Potential
Transmembrane237 – 25721Helical; Name=2; Potential
Topological domain258 – 28730Extracellular Potential
Transmembrane288 – 30821Helical; Name=3; Potential
Topological domain309 – 32719Cytoplasmic Potential
Transmembrane328 – 34821Helical; Name=4; Potential
Topological domain349 – 37325Extracellular Potential
Transmembrane374 – 39421Helical; Name=5; Potential
Topological domain395 – 41925Cytoplasmic Potential
Transmembrane420 – 44021Helical; Name=6; Potential
Topological domain441 – 47636Extracellular Potential
Transmembrane477 – 49721Helical; Name=7; Potential
Topological domain498 – 664167Cytoplasmic Potential
Domain22 – 135114FZ
Motif501 – 5066Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members By similarity

Amino acid modifications

Glycosylation411N-linked (GlcNAc...) Potential
Glycosylation3551N-linked (GlcNAc...) Potential
Disulfide bond27 ↔ 88 By similarity
Disulfide bond35 ↔ 81 By similarity
Disulfide bond72 ↔ 109 By similarity
Disulfide bond98 ↔ 132 By similarity
Disulfide bond102 ↔ 126 By similarity

Experimental info

Mutagenesis5011K → M: Complete loss of activity to induce the Wnt target gene Siamois expression.
Mutagenesis5021T → V: Complete loss of activity to induce the Wnt target gene Siamois expression.
Mutagenesis5031C → S: No effect.
Mutagenesis5061W → G: Complete loss of activity to induce the Wnt target gene Siamois expression.
Mutagenesis5071A → V: No effect.
Mutagenesis5081S → A: No effect.
Mutagenesis5091F → L: No effect.

Sequences

Sequence LengthMass (Da)Tools
O42579 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 51BCC43BDA1791E5

FASTA66475,619
        10         20         30         40         50         60 
MAAYLISFIW VSVILAQKSM GHSLFACEPI TLRMCQDLPY NSTFMPNLLN HYDQQTAALA 

        70         80         90        100        110        120 
MEPFHPMVNL ECSRDLRPFL CALYTPVCME YGRMTLPCRK LCQRAYNECF KLMEMFGVPW 

       130        140        150        160        170        180 
PEEMECSRFP DCDEPYPRIV DISLSGEPSE ETPLAVQRDY GFWCPRELKI DPDLRSSFLG 

       190        200        210        220        230        240 
VRDCSPPCPH MYFRREELSF ARYFIGVISI VCLSATLFTF LTFLIDVTRF RYPERPIIFY 

       250        260        270        280        290        300 
AVCYMMVSLI FFIGFLLEDK VACNGANPSQ YKASTVTQGS HNKACTMLFM VLYFFTMAGS 

       310        320        330        340        350        360 
VWWVILTITW FLAAVPKWGS EAIEKKALLF HASAWGIPGT LTIILLAMNK IEGDNISGVC 

       370        380        390        400        410        420 
FVGLYDVHAL RYFVLAPLCL DVVVGVSLLL AGIISLNRVR IEIPLEKENQ DKLVKFMIRI 

       430        440        450        460        470        480 
GVFSILYLVP LLVVIGCYFY EQAYRGVWET TWVQERCREY HIPCPYKVTQ TSRPDLILFL 

       490        500        510        520        530        540 
MKYLMLLVVG IPSVFWVGSK KTCFEWASFF HGRKKKAGVN ESRQVLQEPD FAQSLLRDPN 

       550        560        570        580        590        600 
TPIVRKSRGT STQGTSTHAS STQLAMLDDQ RSKAGSVQSK VSSYHGSLHR SRDGRYTPCS 

       610        620        630        640        650        660 
YRGIEERLPH GSMSHLTDHS RHSSTHRLNE QSHQGSIRDL SNPLAHISHG TSMNRVIEAD 


ATSA 

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References

[1]"Expression of Xfz3, a Xenopus frizzled family member, is restricted to the early nervous system."
Shi D.-L., Goisset C., Boucaut J.-C.
Mech. Dev. 70:35-47(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[2]"The C-terminal cytoplasmic Lys-Thr-X-X-X-Trp motif in frizzled receptors mediates Wnt/beta-catenin signalling."
Umbhauer M., Djiane A., Goisset C., Penzo-Mendez A., Riou J.-F., Boucaut J.-C., Shi D.-L.
EMBO J. 19:4944-4954(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS, DOMAIN K-T-X-X-X-W MOTIF, COUPLING TO BETA-CATENIN PATHWAY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ001754 mRNA. Translation: CAA04977.1.
RefSeqNP_001083918.1. NM_001090449.1.
UniGeneXl.42572.

3D structure databases

ProteinModelPortalO42579.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-216470.

Protein family/group databases

GPCRDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID399190.
KEGGxla:399190.

Organism-specific databases

CTD7976.
XenbaseXB-GENE-865070. fzd3.

Phylogenomic databases

HOVERGENHBG006977.
KOK02329.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026553. FZD3_vertebrates.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF22. PTHR11309:SF22. 1 hit.
PfamPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSPR00489. FRIZZLED.
SMARTSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFZD3_XENLA
AccessionPrimary (citable) accession number: O42579
Entry history
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries