Reviewed, UniProtKB/Swiss-Prot O42479 (HEMH_CHICK)

Names and origin

Protein names

Recommended name:
    Ferrochelatase, mitochondrial
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:

FECH

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX.
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+.
Cofactor	Binds 1 2Fe-2S cluster.
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ferrochelatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion Potential
	Chain	34 – 402	369	Ferrochelatase, mitochondrial		PRO_0000008875
Sites
	Active site	209	1	By similarity
	Active site	362	1	By similarity
	Metal binding	175	1	Iron-sulfur (2Fe-2S)
	Metal binding	382	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	385	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	390	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O42479-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 3C30BA86CE1C3993	FASTA	402	45,430
	10 20 30 40 50 60 MAAAGRAARP LVAGGRQLRV PLRWRGQVAA AAPSTKPQAE PETRKPKTGI LMLNMGGPER 70 80 90 100 110 120 LDDVHDFLLR LFLDRDLMTL PAQNKLAPFI AKRRTPRIQE QYSRIGGGSP IKKWTAVQGE 130 140 150 160 170 180 GMVKLLDSMS PQTAPHKYYI GFRYVHPLTE EAIEEMEDDG IERAIAFTQY PQYSCSTTGS 190 200 210 220 230 240 SLNAIYRYYN KKGKKPKMKW SIIDRWPTHP LLIQCFADHI QKELDLFPPD KRKDVVILFS 250 260 270 280 290 300 AHSLPMSVVN RGDPYPQEVG ATVQRVMEKL NHSNPYRLVW QSKVGPMPWL VPQTDETIKG 310 320 330 340 350 360 LCQRGKKNML LVPIAFTSDH IETLYELDIE YAQVLANECG VENIRRAESL NGNPLFSKAL 370 380 390 400 ADLVCSHIQS NEICSKQLTL CCPLCVNPVC RETKAFFTNQ QL

References

[1]

"Cloning and characterization of Gallus and Xenopus ferrochelatases: presence of the [2Fe-2S] cluster in nonmammalian ferrochelatase." Day A.L., Parsons B.M., Dailey H.A. Arch. Biochem. Biophys. 359:160-169(1998) [PubMed: 9808757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: White leghorn.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U68033 mRNA. Translation: AAB66503.1.
IPI	IPI00601825.
RefSeq	NP_989527.1.
UniGene	Gga.166
3D structure databases
HSSP	HSSP built from PDB template 1HRK based on UniProtKB P22830.
SMR	O42479. Positions 44-402.
ModBase	Search...
Protein-protein interaction databases
STRING	O42479.
Genome annotation databases
Ensembl	ENSGALT00000004841; ENSGALP00000004832; ENSGALG00000003066; Gallus gallus. [Genome view] ENSGALT00000029413; ENSGALP00000028757; ENSGALG00000003066; Gallus gallus. [Genome view]
GeneID	374020.
KEGG	gga:374020.
Organism-specific databases
CTD	374020.
Phylogenomic databases
HOVERGEN	O42479.
Enzyme and pathway databases
BRENDA	4.99.1.1. 4.
Family and domain databases
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_CHICK

Accession

Primary (citable) accession number: O42479

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	September 22, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)