Reviewed, UniProtKB/Swiss-Prot O42457 (CP1A1_SPAAU)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 1A1
    EC=1.14.14.1
Alternative name(s):
    CYPIA1

Gene names

Name:

cyp1a1

Organism

Sparus aurata (Gilthead sea bream)

Taxonomic identifier

8175 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Perciformes › Percoidei › Sparidae › Sparus

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	Cytochrome P450 1A1		PRO_0000051647
Sites
	Metal binding	463	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	175 – 177	3	LVK → GTR Ref.2
	Sequence conflict	209	1	G → A in AAB62887. Ref.2
	Sequence conflict	212	1	Y → S in AAB62887. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O42457-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 8FE00D86460B303B	FASTA	521	59,122
	10 20 30 40 50 60 MVLMILPFVG PVSVSESLVA IITMCLVYMI LKFFRTEIPE GLCQLPGPKP LPIIGNVLEV 70 80 90 100 110 120 GRNPYLSLTA MSKRYGDVFQ IQIGMRPVVV LSGSETVRQA LIKQGDDFAG RPDLYSFRFI 130 140 150 160 170 180 NDGKSLAFST DQAGVWRARR KLAYSALRSF STLEGTTPEY SCALEEHVSK EAEYLVKQLN 190 200 210 220 230 240 TVMETDGSFD PFRHIVVSVA NVICGMCFGR RYDHNNQELL NLVNLSDEFG QVVASGNPAD 250 260 270 280 290 300 FIPILQYLPS TSMKKFVSIN DRFNAFVQKI VSEHYTTFDK DNIRDITDSL IDHCEDRKLD 310 320 330 340 350 360 ENSNVQMSDE KVVGIVNDLF GAGFDTISTA LSWSVMYLVA YPEIQERLYQ EMKESVGLDR 370 380 390 400 410 420 TPCLSDKPKL PFLEAFILEI FRHSSFLPFT IPHCSSKDTS LNGYFIPKDT CVFINQWQIN 430 440 450 460 470 480 HDPELWKDPS SFNPDRFLNT DGTELNKLEG EKMMVFGLGK RRCIGEVIAR NEVFLFLAIL 490 500 510 520 VQNLRFHAKP GEPLDMTPEY GLTMKHKRCH LRAAMRSRNE E

References

[1]	Cousinou M., Lopez-Barea J., Dorado G. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	Tom M. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 175-521. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF011223 mRNA. Translation: AAB64297.1. AF005719 mRNA. Translation: AAB62887.1.
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBase	Search...
Phylogenomic databases
HOVERGEN	O42457.
Enzyme and pathway databases
BRENDA	1.14.14.1. 191612.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit. PTHR19383:SF63. Cyt_P450_E_grp-I_CYP1. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP1A1_SPAAU

Accession

Primary (citable) accession number: O42457
Secondary accession number(s): O42458

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	October 13, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)