O42449 (IOD1_ORENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type I iodothyronine deiodinase
EC=1.97.1.10

Alternative name(s):
5DI
DIOI
Type 1 DI
Type-I 5'-deiodinase

Gene names

Name:

dio1

Organism

Oreochromis niloticus (Nile tilapia) (Tilapia nilotica) [Reference proteome]

Taxonomic identifier

8128 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Neognathi › Neoteleostei › Eurypterygii › Ctenosquamata › Acanthomorpha › Euacanthomorpha › Holacanthopterygii › Acanthopterygii › Euacanthopterygii › Percomorpha › Perciformes › Labroidei › Cichlidae › African cichlids › Pseudocrenilabrinae › Tilapiini › Oreochromis

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine).
Catalytic activity	3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the iodothyronine deiodinase family.

Ontologies

Keywords
Biological process	Thyroid hormones biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Selenocysteine
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	thyroxine 5'-deiodinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Type I iodothyronine deiodinase		PRO_0000154316
Regions
	Transmembrane	7 – 29	23	Helical; Potential
Sites
	Active site	126	1
Amino acid modifications
	Non-standard residue	126	1	Selenocysteine

Sequences

	Sequence		Length	Mass (Da)
	O42449 [UniParc]. Last modified February 26, 2008. Version 3. Checksum: EB42DE7138551F87	FASTA	248	28,210
	10 20 30 40 50 60 MFLQKIVLYL STACMFCYML GKFLMLVILQ FFSPSLAKKF ILRMGEKITM TQNPRFNYED 70 80 90 100 110 120 WGLTFMSLAF IKTASSHMWL SLGEEAFVGG EAPDSPVVTM DREKTSISKY LKGNRPLVLS 130 140 150 160 170 180 FGSCTUPPFM YKLDEFKQLV KDFSDVADFL VIYIAEAHST DGWAFKNNYD INQHQSLEDR 190 200 210 220 230 240 LSAAQVLVQS EPLCPVVVDE MTDVTTIKYG ALPERLFILQ AGKVLYKGGK GPWGYNPAEV RSFLEKIK

References

[1]

"Characterization of a propylthiouracil-insensitive type I iodothyronine deiodinase." Sanders J.P., van der Geyten S., Kaptein E., Darras V.M., Kuehn E.R., Leonard J.L., Visser T.J. Endocrinology 138:5153-5160(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y11109 mRNA. Translation: CAA71995.1.
RefSeq	XP_003439849.1. XM_003439801.1.
UniGene	Oni.1839.
3D structure databases
ProteinModelPortal	O42449.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100534571.
Organism-specific databases
CTD	1733.
Phylogenomic databases
HOVERGEN	HBG000099.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR000643. Iodothyronine_deiodinase. IPR008261. Iodothyronine_deiodinase_AS. IPR027252. Iodothyronine_deiodinase_I/III. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11781. PTHR11781. 1 hit.
Pfam	PF00837. T4_deiodinase. 1 hit. [Graphical view]
PIRSF	PIRSF001330. IOD. 1 hit. PIRSF500144. IODI_III. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS01205. T4_DEIODINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOD1_ORENI

Accession

Primary (citable) accession number: O42449

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 26, 2008
Last modified:	April 3, 2013
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program