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Protein

Deoxyribonuclease-1

Gene

dnase1

Organism
Oreochromis mossambicus (Mozambique tilapia) (Tilapia mossambica)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed:9395327). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (By similarity). Binds specifically to G-actin and blocks actin polymerization. Preferentially attacks double-stranded DNA and produces oligonucleotides with 5'-phospho and 3'-hydroxy termini (PubMed:9395327).By similarity1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.1 Publication

Cofactori

Ca2+1 Publication, Mg2+1 PublicationNote: Divalent metal cations. Prefers Ca2+ or Mg2+ which have a synergistic effect on activation.1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei90Nitration by tetranitromethane destroys a Ca(2+) binding site and inactivates enzymeBy similarity1
Sitei92Involved in actin-bindingBy similarity1
Active sitei103By similarity1
Active sitei159By similarity1

GO - Molecular functioni

GO - Biological processi

  • DNA catabolic process, endonucleolytic Source: UniProtKB
  • neutrophil activation involved in immune response Source: UniProtKB
  • regulation of acute inflammatory response Source: UniProtKB
  • regulation of neutrophil mediated cytotoxicity Source: UniProtKB

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandCalcium

Enzyme and pathway databases

BRENDAi3.1.21.1 4428

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease-1 (EC:3.1.21.11 Publication)
Alternative name(s):
Deoxyribonuclease I
Short name:
DNase I
Gene namesi
Name:dnase1
OrganismiOreochromis mossambicus (Mozambique tilapia) (Tilapia mossambica)
Taxonomic identifieri8127 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaCichlomorphaeCichliformesCichlidaeAfrican cichlidsPseudocrenilabrinaeOreochrominiOreochromis

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000000728327 – 284Deoxyribonuclease-1Add BLAST258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi44N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi198 ↔ 234Essential for enzymatic activityBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO42446

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO42446
SMRiO42446
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNase I family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG051368

Family and domain databases

Gene3Di3.60.10.101 hit
InterProiView protein in InterPro
IPR018057 Deoxyribonuclease-1_AS
IPR016202 DNase_I
IPR033125 DNASE_I_2
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
PIRSFiPIRSF000988 DNase_I_euk, 1 hit
PRINTSiPR00130 DNASEI
SMARTiView protein in SMART
SM00476 DNaseIc, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit
PROSITEiView protein in PROSITE
PS00919 DNASE_I_1, 1 hit
PS00918 DNASE_I_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O42446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTYRSRMHL VCSLGLFLTL LHLSNSLLLG AFNIKSFGDT KASNATLMNI
60 70 80 90 100
ITKIVKRYDV ILIQEVRDSD LSATQTLMNY VNKDSPQYKY IVSEPLGAST
110 120 130 140 150
YKERYLFLYR EALVSVVKSY TYDDGPEETG QDTFSREPFV VMFSSKNTAV
160 170 180 190 200
RDFTLIPQHT SPDLAVRELN ALYDVVLDVR ARWNTNDIVL LGDFNAGCSY
210 220 230 240 250
VSGSAWQQIR IFTDKTFHWL ITDAADTTVS QTVCPYDRIV VTTDMMRGVV
260 270 280
QNSAKVYNYM TDLNLKQDLA LAVSDHFPVE VKLS
Length:284
Mass (Da):32,216
Last modified:August 1, 1999 - v2
Checksum:iD5154AF0C782B595
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti151R → K1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001305 mRNA Translation: CAA04665.1

Similar proteinsi

Entry informationi

Entry nameiDNAS1_OREMO
AccessioniPrimary (citable) accession number: O42446
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1999
Last modified: February 28, 2018
This is version 92 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing