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Protein

Neurofascin

Gene

NFASC

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofascin
Gene namesi
Name:NFASC
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 1235ExtracellularSequence analysisAdd BLAST1210
Transmembranei1236 – 1256HelicalSequence analysisAdd BLAST21
Topological domaini1257 – 1369CytoplasmicSequence analysisAdd BLAST113

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000001504826 – 1369NeurofascinAdd BLAST1344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi64 ↔ 119PROSITE-ProRule annotation
Disulfide bondi163 ↔ 214PROSITE-ProRule annotation
Glycosylationi241N-linked (GlcNAc...)Sequence analysis1
Glycosylationi247N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi286 ↔ 334PROSITE-ProRule annotation
Glycosylationi323N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi376 ↔ 426PROSITE-ProRule annotation
Glycosylationi427N-linked (GlcNAc...)Sequence analysis1
Glycosylationi464N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi470 ↔ 519PROSITE-ProRule annotation
Glycosylationi501N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi561 ↔ 610PROSITE-ProRule annotation
Glycosylationi692N-linked (GlcNAc...)Sequence analysis1
Glycosylationi767N-linked (GlcNAc...)Sequence analysis1
Glycosylationi793N-linked (GlcNAc...)Sequence analysis1
Glycosylationi853N-linked (GlcNAc...)Sequence analysis1
Glycosylationi994N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1009N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1133N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1150N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1156N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1171N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated and O-glycosylated.1 Publication
May be proteolytically cleaved at Arg-636.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei636 – 637CleavageCurated2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO42414.
PRIDEiO42414.

Expressioni

Developmental stagei

There is one major 'early' isoform and multiple 'late' isoforms. Around 50 isoforms are found at different developmental stages.

Interactioni

Protein-protein interaction databases

BioGridi680872. 2 interactors.
IntActiO42414. 1 interactor.
STRINGi9031.ENSGALP00000034591.

Structurei

3D structure databases

ProteinModelPortaliO42414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 138Ig-like C2-type 1Add BLAST97
Domaini144 – 231Ig-like C2-type 2Add BLAST88
Domaini262 – 350Ig-like C2-type 3Add BLAST89
Domaini355 – 442Ig-like C2-type 4Add BLAST88
Domaini448 – 535Ig-like C2-type 5Add BLAST88
Domaini539 – 626Ig-like C2-type 6Add BLAST88
Domaini645 – 740Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST96
Domaini745 – 838Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST94
Domaini843 – 945Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST103
Domaini949 – 1057Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST109
Domaini1133 – 1222Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST90

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1052 – 1126Thr-richAdd BLAST75

Sequence similaritiesi

Contains 5 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3513. Eukaryota.
ENOG410XSVG. LUCA.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiO42414.
KOiK06757.
PhylomeDBiO42414.

Family and domain databases

CDDicd00063. FN3. 5 hits.
Gene3Di2.60.40.10. 11 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013151. Immunoglobulin.
IPR026966. Neurofascin/L1/NrCAM_C.
[Graphical view]
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 3 hits.
PF07679. I-set. 2 hits.
PF00047. ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 5 hits.
SM00409. IG. 6 hits.
SM00408. IGc2. 6 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
SSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 5 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: O42414-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLHSHQLTY AGIAFALCLH HLISAIEVPL DSNIQSELPQ PPTITKQSVK
60 70 80 90 100
DYIVDPRDNI FIECEAKGNP VPTFSWTRNG KFFNVAKDPK VSMRRRSGTL
110 120 130 140 150
VIDFHGGGRP DDYEGEYQCF ARNDYGTALS SKIHLQVSRS PLWPKEKVDV
160 170 180 190 200
IEVDEGAPLS LQCNPPPGLP PPVIFWMSSS MEPIHQDKRV SQGQNGDLYF
210 220 230 240 250
SNVMLQDAQT DYSCNARFHF THTIQQKNPY TLKVKTKKPH NETSLRNHTD
260 270 280 290 300
MYSARGVTET TPSFMYPYGT SSSQMVLRGV DLLLECIASG VPAPDIMWYK
310 320 330 340 350
KGGELPAGKT KLENFNKALR ISNVSEEDSG EYFCLASNKM GSIRHTISVR
360 370 380 390 400
VKAAPYWLDE PQNLILAPGE DGRLVCRANG NPKPSIQWLV NGEPIEGSPP
410 420 430 440 450
NPSREVAGDT IVFRDTQIGS SAVYQCNASN EHGYLLANAF VSVLDVPPRI
460 470 480 490 500
LAPRNQLIKV IQYNRTRLDC PFFGSPIPTL RWFKNGQGNM LDGGNYKAHE
510 520 530 540 550
NGSLEMSMAR KEDQGIYTCV ATNILGKVEA QVRLEVKDPT RIVRGPEDQV
560 570 580 590 600
VKRGSMPRLH CRVKHDPTLK LTVTWLKDDA PLYIGNRMKK EDDGLTIYGV
610 620 630 640 650
AEKDQGDYTC VASTELDKDS AKAYLTVLAI PANRLRDLPK ERPDRPRDLE
660 670 680 690 700
LSDLAERSVK LTWIPGDDNN SPITDYIVQF EEDRFQPGTW HNHSRYPGNV
710 720 730 740 750
NSALLSLSPY VNYQFRVIAV NDVGSSLPSM PSERYQTSGA RPEINPTGVQ
760 770 780 790 800
GAGTQKNNME ITWTPLNATQ AYGPNLRYIV RWRRRDPRGS WYNETVKAPR
810 820 830 840 850
HVVWNTPIYV PYEIKVQAEN DFGRAPEPET YIGYSGEDYP KAAPTDVRIR
860 870 880 890 900
VLNSTAIALT WTRVHLDTIQ GQLKEYRAYF WRDSSLLKNL WVSKKRQYVS
910 920 930 940 950
FPGDRNRGIV SRLFPYSNYK LEMVVTNGRG DGPRSEVKEF PTPEGVPSSP
960 970 980 990 1000
RYLRIRQPNL ESINLEWDHP EHPNGVLTGY NLRYQASCLS SPVNGSKTGR
1010 1020 1030 1040 1050
TLVENFSPNQ TRFTVQRTDP ISRYRFFLRA RTQVGDGEVI VEESPALLNE
1060 1070 1080 1090 1100
ATPTPASTWL PPPTTELTPA ATIATTTTTA TPTTETPPTE IPTTAIPTTT
1110 1120 1130 1140 1150
TTTTTTAAST VASTTTTAER AAAATTKQEL ATNGSSIWDI RAWANSNWAN
1160 1170 1180 1190 1200
ITWSHNYSAG TDFVVKYITS NKTEKSIPVK AQTPSSVQLA NLTPGMVYKL
1210 1220 1230 1240 1250
WVFPIWSSPS EHSYITFTTS SAYTKNHVDI ATQGWFIGLM CAIALLVLIL
1260 1270 1280 1290 1300
LIVCFIKRSR GGKYPVRDNK DEHLNPEDKN VEDGSFDYRS LESDEDNKPL
1310 1320 1330 1340 1350
PNSQTSLDGT IKQQESDDSL VDYGEGGEGQ FNEDGSFIGQ YTVKKDKEET
1360
EGNESSEATS PVNAIYSLA
Length:1,369
Mass (Da):152,955
Last modified:January 1, 1998 - v1
Checksum:i835F27D086B2BFF6
GO
Isoform 2 (identifier: O42414-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     987-993: SCLSSPV → F
     1132-1222: Missing.

Show »
Length:1,272
Mass (Da):142,273
Checksum:iBD101CAEAA3E16E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1105T → A in CAA46330 (PubMed:9353344).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008935987 – 993SCLSSPV → F in isoform 2. 1 Publication7
Alternative sequenceiVSP_0089361132 – 1222Missing in isoform 2. 1 PublicationAdd BLAST91

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65224 mRNA. Translation: CAA46330.1.
Y14341
, Y14342, Y14343, Y14344, Y14345, Y14346, Y14347, Y14348, Y14349, Y14350, Y14351, Y14352, Y14353, Y14354 Genomic DNA. Translation: CAA74726.1.
PIRiS26180.
RefSeqiNP_001004493.1. NM_001004493.4. [O42414-2]
UniGeneiGga.4889.

Genome annotation databases

GeneIDi419824.
KEGGigga:419824.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65224 mRNA. Translation: CAA46330.1.
Y14341
, Y14342, Y14343, Y14344, Y14345, Y14346, Y14347, Y14348, Y14349, Y14350, Y14351, Y14352, Y14353, Y14354 Genomic DNA. Translation: CAA74726.1.
PIRiS26180.
RefSeqiNP_001004493.1. NM_001004493.4. [O42414-2]
UniGeneiGga.4889.

3D structure databases

ProteinModelPortaliO42414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi680872. 2 interactors.
IntActiO42414. 1 interactor.
STRINGi9031.ENSGALP00000034591.

Proteomic databases

PaxDbiO42414.
PRIDEiO42414.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi419824.
KEGGigga:419824.

Organism-specific databases

CTDi23114.

Phylogenomic databases

eggNOGiKOG3513. Eukaryota.
ENOG410XSVG. LUCA.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiO42414.
KOiK06757.
PhylomeDBiO42414.

Miscellaneous databases

PROiO42414.

Family and domain databases

CDDicd00063. FN3. 5 hits.
Gene3Di2.60.40.10. 11 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013151. Immunoglobulin.
IPR026966. Neurofascin/L1/NrCAM_C.
[Graphical view]
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 3 hits.
PF07679. I-set. 2 hits.
PF00047. ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 5 hits.
SM00409. IG. 6 hits.
SM00408. IGc2. 6 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
SSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 5 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNFASC_CHICK
AccessioniPrimary (citable) accession number: O42414
Secondary accession number(s): Q90924
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.