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Protein

Neurofascin

Gene

NFASC

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofascin
Gene namesi
Name:NFASC
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 12351210ExtracellularSequence analysisAdd
BLAST
Transmembranei1236 – 125621HelicalSequence analysisAdd
BLAST
Topological domaini1257 – 1369113CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 13691344NeurofascinPRO_0000015048Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi64 ↔ 119PROSITE-ProRule annotation
Disulfide bondi163 ↔ 214PROSITE-ProRule annotation
Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence analysis
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence analysis
Disulfide bondi286 ↔ 334PROSITE-ProRule annotation
Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence analysis
Disulfide bondi376 ↔ 426PROSITE-ProRule annotation
Glycosylationi427 – 4271N-linked (GlcNAc...)Sequence analysis
Glycosylationi464 – 4641N-linked (GlcNAc...)Sequence analysis
Disulfide bondi470 ↔ 519PROSITE-ProRule annotation
Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence analysis
Disulfide bondi561 ↔ 610PROSITE-ProRule annotation
Glycosylationi692 – 6921N-linked (GlcNAc...)Sequence analysis
Glycosylationi767 – 7671N-linked (GlcNAc...)Sequence analysis
Glycosylationi793 – 7931N-linked (GlcNAc...)Sequence analysis
Glycosylationi853 – 8531N-linked (GlcNAc...)Sequence analysis
Glycosylationi994 – 9941N-linked (GlcNAc...)Sequence analysis
Glycosylationi1009 – 10091N-linked (GlcNAc...)Sequence analysis
Glycosylationi1133 – 11331N-linked (GlcNAc...)Sequence analysis
Glycosylationi1150 – 11501N-linked (GlcNAc...)Sequence analysis
Glycosylationi1156 – 11561N-linked (GlcNAc...)Sequence analysis
Glycosylationi1171 – 11711N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated and O-glycosylated.1 Publication
May be proteolytically cleaved at Arg-636.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei636 – 6372CleavageCurated

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO42414.
PRIDEiO42414.

Expressioni

Developmental stagei

There is one major 'early' isoform and multiple 'late' isoforms. Around 50 isoforms are found at different developmental stages.

Interactioni

Protein-protein interaction databases

BioGridi680872. 2 interactions.
IntActiO42414. 1 interaction.
STRINGi9031.ENSGALP00000034591.

Structurei

3D structure databases

ProteinModelPortaliO42414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 13897Ig-like C2-type 1Add
BLAST
Domaini144 – 23188Ig-like C2-type 2Add
BLAST
Domaini262 – 35089Ig-like C2-type 3Add
BLAST
Domaini355 – 44288Ig-like C2-type 4Add
BLAST
Domaini448 – 53588Ig-like C2-type 5Add
BLAST
Domaini539 – 62688Ig-like C2-type 6Add
BLAST
Domaini645 – 74096Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini745 – 83894Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini843 – 945103Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini949 – 1057109Fibronectin type-III 4PROSITE-ProRule annotationAdd
BLAST
Domaini1133 – 122290Fibronectin type-III 5PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1052 – 112675Thr-richAdd
BLAST

Sequence similaritiesi

Contains 5 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3513. Eukaryota.
ENOG410XSVG. LUCA.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiO42414.
KOiK06757.
PhylomeDBiO42414.

Family and domain databases

Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013151. Immunoglobulin.
IPR026966. Neurofascin/L1/NrCAM_C.
[Graphical view]
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 3 hits.
PF07679. I-set. 2 hits.
PF00047. ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 5 hits.
SM00409. IG. 6 hits.
SM00408. IGc2. 6 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
SSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 5 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.

Isoform 1 (identifier: O42414-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLHSHQLTY AGIAFALCLH HLISAIEVPL DSNIQSELPQ PPTITKQSVK
60 70 80 90 100
DYIVDPRDNI FIECEAKGNP VPTFSWTRNG KFFNVAKDPK VSMRRRSGTL
110 120 130 140 150
VIDFHGGGRP DDYEGEYQCF ARNDYGTALS SKIHLQVSRS PLWPKEKVDV
160 170 180 190 200
IEVDEGAPLS LQCNPPPGLP PPVIFWMSSS MEPIHQDKRV SQGQNGDLYF
210 220 230 240 250
SNVMLQDAQT DYSCNARFHF THTIQQKNPY TLKVKTKKPH NETSLRNHTD
260 270 280 290 300
MYSARGVTET TPSFMYPYGT SSSQMVLRGV DLLLECIASG VPAPDIMWYK
310 320 330 340 350
KGGELPAGKT KLENFNKALR ISNVSEEDSG EYFCLASNKM GSIRHTISVR
360 370 380 390 400
VKAAPYWLDE PQNLILAPGE DGRLVCRANG NPKPSIQWLV NGEPIEGSPP
410 420 430 440 450
NPSREVAGDT IVFRDTQIGS SAVYQCNASN EHGYLLANAF VSVLDVPPRI
460 470 480 490 500
LAPRNQLIKV IQYNRTRLDC PFFGSPIPTL RWFKNGQGNM LDGGNYKAHE
510 520 530 540 550
NGSLEMSMAR KEDQGIYTCV ATNILGKVEA QVRLEVKDPT RIVRGPEDQV
560 570 580 590 600
VKRGSMPRLH CRVKHDPTLK LTVTWLKDDA PLYIGNRMKK EDDGLTIYGV
610 620 630 640 650
AEKDQGDYTC VASTELDKDS AKAYLTVLAI PANRLRDLPK ERPDRPRDLE
660 670 680 690 700
LSDLAERSVK LTWIPGDDNN SPITDYIVQF EEDRFQPGTW HNHSRYPGNV
710 720 730 740 750
NSALLSLSPY VNYQFRVIAV NDVGSSLPSM PSERYQTSGA RPEINPTGVQ
760 770 780 790 800
GAGTQKNNME ITWTPLNATQ AYGPNLRYIV RWRRRDPRGS WYNETVKAPR
810 820 830 840 850
HVVWNTPIYV PYEIKVQAEN DFGRAPEPET YIGYSGEDYP KAAPTDVRIR
860 870 880 890 900
VLNSTAIALT WTRVHLDTIQ GQLKEYRAYF WRDSSLLKNL WVSKKRQYVS
910 920 930 940 950
FPGDRNRGIV SRLFPYSNYK LEMVVTNGRG DGPRSEVKEF PTPEGVPSSP
960 970 980 990 1000
RYLRIRQPNL ESINLEWDHP EHPNGVLTGY NLRYQASCLS SPVNGSKTGR
1010 1020 1030 1040 1050
TLVENFSPNQ TRFTVQRTDP ISRYRFFLRA RTQVGDGEVI VEESPALLNE
1060 1070 1080 1090 1100
ATPTPASTWL PPPTTELTPA ATIATTTTTA TPTTETPPTE IPTTAIPTTT
1110 1120 1130 1140 1150
TTTTTTAAST VASTTTTAER AAAATTKQEL ATNGSSIWDI RAWANSNWAN
1160 1170 1180 1190 1200
ITWSHNYSAG TDFVVKYITS NKTEKSIPVK AQTPSSVQLA NLTPGMVYKL
1210 1220 1230 1240 1250
WVFPIWSSPS EHSYITFTTS SAYTKNHVDI ATQGWFIGLM CAIALLVLIL
1260 1270 1280 1290 1300
LIVCFIKRSR GGKYPVRDNK DEHLNPEDKN VEDGSFDYRS LESDEDNKPL
1310 1320 1330 1340 1350
PNSQTSLDGT IKQQESDDSL VDYGEGGEGQ FNEDGSFIGQ YTVKKDKEET
1360
EGNESSEATS PVNAIYSLA
Length:1,369
Mass (Da):152,955
Last modified:January 1, 1998 - v1
Checksum:i835F27D086B2BFF6
GO
Isoform 2 (identifier: O42414-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     987-993: SCLSSPV → F
     1132-1222: Missing.

Show »
Length:1,272
Mass (Da):142,273
Checksum:iBD101CAEAA3E16E2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1105 – 11051T → A in CAA46330 (PubMed:9353344).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei987 – 9937SCLSSPV → F in isoform 2. 1 PublicationVSP_008935
Alternative sequencei1132 – 122291Missing in isoform 2. 1 PublicationVSP_008936Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65224 mRNA. Translation: CAA46330.1.
Y14341
, Y14342, Y14343, Y14344, Y14345, Y14346, Y14347, Y14348, Y14349, Y14350, Y14351, Y14352, Y14353, Y14354 Genomic DNA. Translation: CAA74726.1.
PIRiS26180.
RefSeqiNP_001004493.1. NM_001004493.4. [O42414-2]
UniGeneiGga.4889.

Genome annotation databases

GeneIDi419824.
KEGGigga:419824.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65224 mRNA. Translation: CAA46330.1.
Y14341
, Y14342, Y14343, Y14344, Y14345, Y14346, Y14347, Y14348, Y14349, Y14350, Y14351, Y14352, Y14353, Y14354 Genomic DNA. Translation: CAA74726.1.
PIRiS26180.
RefSeqiNP_001004493.1. NM_001004493.4. [O42414-2]
UniGeneiGga.4889.

3D structure databases

ProteinModelPortaliO42414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi680872. 2 interactions.
IntActiO42414. 1 interaction.
STRINGi9031.ENSGALP00000034591.

Proteomic databases

PaxDbiO42414.
PRIDEiO42414.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi419824.
KEGGigga:419824.

Organism-specific databases

CTDi23114.

Phylogenomic databases

eggNOGiKOG3513. Eukaryota.
ENOG410XSVG. LUCA.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiO42414.
KOiK06757.
PhylomeDBiO42414.

Miscellaneous databases

PROiO42414.

Family and domain databases

Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013151. Immunoglobulin.
IPR026966. Neurofascin/L1/NrCAM_C.
[Graphical view]
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 3 hits.
PF07679. I-set. 2 hits.
PF00047. ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 5 hits.
SM00409. IG. 6 hits.
SM00408. IGc2. 6 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
SSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 5 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure of the axonal surface recognition molecule neurofascin and its relationship to a neural subgroup of the immunoglobulin superfamily."
    Volkmer H., Hassel B., Wolff J.M., Frank R., Rathjen F.G.
    J. Cell Biol. 118:149-161(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PROTEIN SEQUENCE OF 26-46; 637-641; 717-730; 758-781 AND 801-815, CLEAVAGE AT ARG-636, GLYCOSYLATION.
    Tissue: Brain.
  2. "Organization of the neurofascin gene and analysis of developmentally regulated alternative splicing."
    Hassel B., Rathjen F.G., Volkmer H.
    J. Biol. Chem. 272:28742-28749(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    Tissue: Liver.

Entry informationi

Entry nameiNFASC_CHICK
AccessioniPrimary (citable) accession number: O42414
Secondary accession number(s): Q90924
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.