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O42376

- MK12_DANRE

UniProt

O42376 - MK12_DANRE

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Protein
Mitogen-activated protein kinase 12
Gene
mapk12, sapk3
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Magnesium By similarity.By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATP By similarityBy similarity
Active sitei151 – 1511Proton acceptor By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATP By similarityBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. response to stress Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_224357. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 12 (EC:2.7.11.24)
Short name:
MAP kinase 12
Short name:
MAPK 12
Alternative name(s):
Stress-activated protein kinase 3
Gene namesi
Name:mapk12
Synonyms:sapk3
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 18

Organism-specific databases

ZFINiZDB-GENE-990415-257. mapk12a.

Subcellular locationi

Cytoplasm By similarity By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Mitogen-activated protein kinase 12
PRO_0000186285Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811Phosphothreonine By similarityBy similarity
Modified residuei183 – 1831Phosphotyrosine By similarityBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO42376.

Expressioni

Gene expression databases

BgeeiO42376.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000011298.

Structurei

3D structure databases

ProteinModelPortaliO42376.
SMRiO42376. Positions 6-351.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 309285Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi181 – 1833TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiO42376.
OMAiNFKPPQT.
OrthoDBiEOG7PCJGV.
TreeFamiTF105100.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O42376-1 [UniParc]FASTAAdd to Basket

« Hide

MTARSRPGYF RQEINKTIWE VPDRYKDLKQ VGTGAYGTVC YALDRRTGAK    50
VAIKKLHRPF QSDLFAKRAY RELRLLKHMK HDNVIGLVDV FTADLSLDRF 100
HDFYLVMPFM GTDLGKLMKM ERLSEERVQY LVYQMLKGLK YIHAAGIIHR 150
DLKPGNLAIN EECELKILDF GLARQTDSEM TGYVVTRWYR APEVILSWMH 200
YTQTVDIWSV GCIMAEMLLG KPLFKGHDHL DQLMEIMKVT GTPSKEFTAK 250
LQSEDARNYV TKLPRFRKKD LRILLPNVNP QAIKVLDGML LLDPESRITA 300
AEALAFPFFS EFREPEEETE APPYDHSLDE ADQSLEQWKR LTFTEILTFQ 350
PAPAVAESKE TAL 363
Length:363
Mass (Da):41,971
Last modified:January 1, 1998 - v1
Checksum:i81B06D414254EFBF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y15075 mRNA. Translation: CAA75355.1.
UniGeneiDr.104488.

Genome annotation databases

EnsembliENSDART00000018502; ENSDARP00000011298; ENSDARG00000042021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y15075 mRNA. Translation: CAA75355.1 .
UniGenei Dr.104488.

3D structure databases

ProteinModelPortali O42376.
SMRi O42376. Positions 6-351.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7955.ENSDARP00000011298.

Proteomic databases

PRIDEi O42376.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000018502 ; ENSDARP00000011298 ; ENSDARG00000042021 .

Organism-specific databases

ZFINi ZDB-GENE-990415-257. mapk12a.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
HOVERGENi HBG014652.
InParanoidi O42376.
OMAi NFKPPQT.
OrthoDBi EOG7PCJGV.
TreeFami TF105100.

Enzyme and pathway databases

Reactomei REACT_224357. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.

Gene expression databases

Bgeei O42376.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01773. P38MAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Goedert M.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Somatic embryo.

Entry informationi

Entry nameiMK12_DANRE
AccessioniPrimary (citable) accession number: O42376
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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