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O42376 (MK12_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 12

Short name=MAP kinase 12
Short name=MAPK 12
EC=2.7.11.24
Alternative name(s):
Stress-activated protein kinase 3
Gene names
Name:mapk12
Synonyms:sapk3
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. UniProtKB Q63538

Cofactor

Magnesium By similarity. UniProtKB Q63538

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity. UniProtKB Q63538

Subcellular location

Cytoplasm By similarity UniProtKB Q63538.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 363363Mitogen-activated protein kinase 12
PRO_0000186285

Regions

Domain25 – 309285Protein kinase
Nucleotide binding31 – 399ATP By similarity UniProtKB Q16539
Motif181 – 1833TXY

Sites

Active site1511Proton acceptor By similarity UniProtKB Q16539
Binding site541ATP By similarity UniProtKB Q16539

Amino acid modifications

Modified residue1811Phosphothreonine By similarity UniProtKB Q63538
Modified residue1831Phosphotyrosine By similarity UniProtKB Q63538

Sequences

Sequence LengthMass (Da)Tools
O42376 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 81B06D414254EFBF

FASTA36341,971
        10         20         30         40         50         60 
MTARSRPGYF RQEINKTIWE VPDRYKDLKQ VGTGAYGTVC YALDRRTGAK VAIKKLHRPF 

        70         80         90        100        110        120 
QSDLFAKRAY RELRLLKHMK HDNVIGLVDV FTADLSLDRF HDFYLVMPFM GTDLGKLMKM 

       130        140        150        160        170        180 
ERLSEERVQY LVYQMLKGLK YIHAAGIIHR DLKPGNLAIN EECELKILDF GLARQTDSEM 

       190        200        210        220        230        240 
TGYVVTRWYR APEVILSWMH YTQTVDIWSV GCIMAEMLLG KPLFKGHDHL DQLMEIMKVT 

       250        260        270        280        290        300 
GTPSKEFTAK LQSEDARNYV TKLPRFRKKD LRILLPNVNP QAIKVLDGML LLDPESRITA 

       310        320        330        340        350        360 
AEALAFPFFS EFREPEEETE APPYDHSLDE ADQSLEQWKR LTFTEILTFQ PAPAVAESKE 


TAL 

« Hide

References

[1]Goedert M.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Somatic embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y15075 mRNA. Translation: CAA75355.1.
UniGeneDr.104488.

3D structure databases

ProteinModelPortalO42376.
SMRO42376. Positions 6-351.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000011298.

Proteomic databases

PRIDEO42376.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000018502; ENSDARP00000011298; ENSDARG00000042021.

Organism-specific databases

ZFINZDB-GENE-990415-257. mapk12a.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
HOVERGENHBG014652.
InParanoidO42376.
OMANFKPPQT.
OrthoDBEOG7PCJGV.
TreeFamTF105100.

Gene expression databases

BgeeO42376.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01773. P38MAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMK12_DANRE
AccessionPrimary (citable) accession number: O42376
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families