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Protein

Mitogen-activated protein kinase 12

Gene

mapk12

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATPPROSITE-ProRule annotation
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_298703. p38MAPK events.
REACT_302200. DSCAM interactions.
REACT_308039. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.
REACT_347216. VEGFA-VEGFR2 Pathway.
REACT_353404. CDO in myogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 12 (EC:2.7.11.24)
Short name:
MAP kinase 12
Short name:
MAPK 12
Alternative name(s):
Stress-activated protein kinase 3
Gene namesi
Name:mapk12
Synonyms:sapk3
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Chromosome 18

Organism-specific databases

ZFINiZDB-GENE-990415-257. mapk12a.

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Mitogen-activated protein kinase 12PRO_0000186285Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811PhosphothreonineBy similarity
Modified residuei183 – 1831PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO42376.

Expressioni

Gene expression databases

BgeeiO42376.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000011298.

Structurei

3D structure databases

ProteinModelPortaliO42376.
SMRiO42376. Positions 6-351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 309285Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi181 – 1833TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiO42376.
OMAiNFKPPQT.
OrthoDBiEOG7PCJGV.
TreeFamiTF105100.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O42376-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTARSRPGYF RQEINKTIWE VPDRYKDLKQ VGTGAYGTVC YALDRRTGAK
60 70 80 90 100
VAIKKLHRPF QSDLFAKRAY RELRLLKHMK HDNVIGLVDV FTADLSLDRF
110 120 130 140 150
HDFYLVMPFM GTDLGKLMKM ERLSEERVQY LVYQMLKGLK YIHAAGIIHR
160 170 180 190 200
DLKPGNLAIN EECELKILDF GLARQTDSEM TGYVVTRWYR APEVILSWMH
210 220 230 240 250
YTQTVDIWSV GCIMAEMLLG KPLFKGHDHL DQLMEIMKVT GTPSKEFTAK
260 270 280 290 300
LQSEDARNYV TKLPRFRKKD LRILLPNVNP QAIKVLDGML LLDPESRITA
310 320 330 340 350
AEALAFPFFS EFREPEEETE APPYDHSLDE ADQSLEQWKR LTFTEILTFQ
360
PAPAVAESKE TAL
Length:363
Mass (Da):41,971
Last modified:January 1, 1998 - v1
Checksum:i81B06D414254EFBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15075 mRNA. Translation: CAA75355.1.
UniGeneiDr.104488.

Genome annotation databases

EnsembliENSDART00000018502; ENSDARP00000011298; ENSDARG00000042021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15075 mRNA. Translation: CAA75355.1.
UniGeneiDr.104488.

3D structure databases

ProteinModelPortaliO42376.
SMRiO42376. Positions 6-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000011298.

Proteomic databases

PRIDEiO42376.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000018502; ENSDARP00000011298; ENSDARG00000042021.

Organism-specific databases

ZFINiZDB-GENE-990415-257. mapk12a.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiO42376.
OMAiNFKPPQT.
OrthoDBiEOG7PCJGV.
TreeFamiTF105100.

Enzyme and pathway databases

ReactomeiREACT_298703. p38MAPK events.
REACT_302200. DSCAM interactions.
REACT_308039. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.
REACT_347216. VEGFA-VEGFR2 Pathway.
REACT_353404. CDO in myogenesis.

Miscellaneous databases

PROiO42376.

Gene expression databases

BgeeiO42376.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Goedert M.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Somatic embryo.

Entry informationi

Entry nameiMK12_DANRE
AccessioniPrimary (citable) accession number: O42376
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: January 1, 1998
Last modified: April 29, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.