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Protein

Mitogen-activated protein kinase 12

Gene

mapk12

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei151Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processStress response, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DRE-168638 NOD1/2 Signaling Pathway
R-DRE-375170 CDO in myogenesis
R-DRE-4420097 VEGFA-VEGFR2 Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 12 (EC:2.7.11.24)
Short name:
MAP kinase 12
Short name:
MAPK 12
Alternative name(s):
Stress-activated protein kinase 3
Gene namesi
Name:mapk12
Synonyms:sapk3
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 18

Organism-specific databases

ZFINiZDB-GENE-990415-257 mapk12a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001862851 – 363Mitogen-activated protein kinase 12Add BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181PhosphothreonineBy similarity1
Modified residuei183PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO42376
PRIDEiO42376

Expressioni

Gene expression databases

BgeeiENSDARG00000042021
ExpressionAtlasiO42376 baseline

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000011298

Structurei

3D structure databases

ProteinModelPortaliO42376
SMRiO42376
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 309Protein kinasePROSITE-ProRule annotationAdd BLAST285

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi181 – 183TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
GeneTreeiENSGT00550000074271
HOGENOMiHOG000233024
HOVERGENiHBG014652
InParanoidiO42376
OMAiMSSGAMD
OrthoDBiEOG091G08QL
TreeFamiTF105100

Family and domain databases

CDDicd07880 STKc_p38gamma, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008352 MAPK_p38-like
IPR038786 p38gamma
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01773 P38MAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

O42376-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTARSRPGYF RQEINKTIWE VPDRYKDLKQ VGTGAYGTVC YALDRRTGAK
60 70 80 90 100
VAIKKLHRPF QSDLFAKRAY RELRLLKHMK HDNVIGLVDV FTADLSLDRF
110 120 130 140 150
HDFYLVMPFM GTDLGKLMKM ERLSEERVQY LVYQMLKGLK YIHAAGIIHR
160 170 180 190 200
DLKPGNLAIN EECELKILDF GLARQTDSEM TGYVVTRWYR APEVILSWMH
210 220 230 240 250
YTQTVDIWSV GCIMAEMLLG KPLFKGHDHL DQLMEIMKVT GTPSKEFTAK
260 270 280 290 300
LQSEDARNYV TKLPRFRKKD LRILLPNVNP QAIKVLDGML LLDPESRITA
310 320 330 340 350
AEALAFPFFS EFREPEEETE APPYDHSLDE ADQSLEQWKR LTFTEILTFQ
360
PAPAVAESKE TAL
Length:363
Mass (Da):41,971
Last modified:January 1, 1998 - v1
Checksum:i81B06D414254EFBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15075 mRNA Translation: CAA75355.1
UniGeneiDr.104488

Genome annotation databases

EnsembliENSDART00000018502; ENSDARP00000011298; ENSDARG00000042021

Similar proteinsi

Entry informationi

Entry nameiMK12_DANRE
AccessioniPrimary (citable) accession number: O42376
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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