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Protein

E3 ubiquitin-protein ligase Mdm2

Gene

mdm2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degration by the proteasome.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri274 – 30330RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri392 – 43342RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • negative regulation of apoptotic process Source: ZFIN
  • negative regulation of cell cycle arrest Source: ZFIN
  • negative regulation of transcription from RNA polymerase II promoter Source: InterPro
  • protein ubiquitination Source: InterPro
  • response to X-ray Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Mdm2 (EC:6.3.2.-)
Alternative name(s):
Double minute 2 protein
p53-binding protein Mdm2
Gene namesi
Name:mdm2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-990415-153. mdm2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445E3 ubiquitin-protein ligase Mdm2PRO_0000157335Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO42354.
SMRiO42354. Positions 18-102, 265-307, 384-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 10081SWIBAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni190 – 27990ARF-bindingAdd
BLAST
Regioni222 – 30685Region IIAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi160 – 1667Nuclear localization signalSequence analysis
Motifi171 – 18313Nuclear export signalAdd
BLAST
Motifi420 – 4278Nucleolar localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi141 – 1455Poly-Arg
Compositional biasi210 – 27667Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the MDM2/MDM4 family.Curated
Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 SWIB domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri274 – 30330RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri392 – 43342RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000293341.
HOVERGENiHBG013472.
InParanoidiO42354.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR028340. Mdm2.
IPR015459. MDM2_E3_ligase.
IPR016495. p53_neg-reg_MDM_2/4.
IPR003121. SWIB_MDM2_domain.
IPR001876. Znf_RanBP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR13844:SF15. PTHR13844:SF15. 1 hit.
PfamiPF02201. SWIB. 1 hit.
[Graphical view]
PIRSFiPIRSF500700. MDM2. 1 hit.
PIRSF006748. p53_MDM_2/4. 1 hit.
SUPFAMiSSF47592. SSF47592. 1 hit.
SSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O42354-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATESCLSSS QISKVDNEKL VRPKVQLKSL LEDAGADKDV FTMKEVMFYL
60 70 80 90 100
GKYIMSKELY DKQQQHIVHC GEDPLGAVLG VKSFSVKEPR ALFALINRNL
110 120 130 140 150
VTVKNPESQS TFSEPRSQSE PDRGPGDTDS DSRSSTSQQQ RRRRRSSDPE
160 170 180 190 200
SSSAEDESRE RRKRHKSDSF SLTFDDSLSW CVIGGLHRER GNSESSDANS
210 220 230 240 250
NSDVGISRSE GSEESEDSDS DSDNFSVEFE VESINSDAYS ENDVDSVPGE
260 270 280 290 300
NEIYEVTIFA EDEDSFDEDT EITEADYWKC PKCDQFNPPL PRHCKSCWTV
310 320 330 340 350
RADWLPETHS NWENLSRNTR TNPEDTSVTT TPNTTFEKKL SKPSSPLPET
360 370 380 390 400
DDGVDVPTPP LLRRGSSQEE TPELERFNSL EACLPATCLE PCVICQSRPK
410 420 430 440
NGCIVHGRTG HLMACYTCAK KLKNRNKLCP VCREPIQSVV LTYMS
Length:445
Mass (Da):49,950
Last modified:January 1, 1998 - v1
Checksum:i6FA8175A8A8E6261
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010255 mRNA. Translation: AAB64176.1.
AF356346 mRNA. Translation: AAM00198.1.
UniGeneiDr.75764.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010255 mRNA. Translation: AAB64176.1.
AF356346 mRNA. Translation: AAM00198.1.
UniGeneiDr.75764.

3D structure databases

ProteinModelPortaliO42354.
SMRiO42354. Positions 18-102, 265-307, 384-443.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ZFINiZDB-GENE-990415-153. mdm2.

Phylogenomic databases

HOGENOMiHOG000293341.
HOVERGENiHBG013472.
InParanoidiO42354.

Miscellaneous databases

PROiO42354.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR028340. Mdm2.
IPR015459. MDM2_E3_ligase.
IPR016495. p53_neg-reg_MDM_2/4.
IPR003121. SWIB_MDM2_domain.
IPR001876. Znf_RanBP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR13844:SF15. PTHR13844:SF15. 1 hit.
PfamiPF02201. SWIB. 1 hit.
[Graphical view]
PIRSFiPIRSF500700. MDM2. 1 hit.
PIRSF006748. p53_MDM_2/4. 1 hit.
SUPFAMiSSF47592. SSF47592. 1 hit.
SSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Partial cDNA nucleotide sequence of the zebrafish homolog of Mdm2."
    Neel H., Piette J.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A gene encoding MDM2-like protein from zebrafish."
    Lin Y.T., Chou C.M., Leu J.H., Tsai S.C., Huang C.J.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiMDM2_DANRE
AccessioniPrimary (citable) accession number: O42354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: May 11, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.