Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase Mdm2

Gene

mdm2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degration by the proteasome.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri274 – 303RanBP2-typePROSITE-ProRule annotationAdd BLAST30
Zinc fingeri392 – 433RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

  • negative regulation of apoptotic process Source: ZFIN
  • negative regulation of cell cycle arrest Source: ZFIN
  • negative regulation of epithelial cell apoptotic process Source: ZFIN
  • negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source: ZFIN
  • negative regulation of transcription from RNA polymerase II promoter Source: InterPro
  • pronephric glomerulus morphogenesis Source: ZFIN
  • protein ubiquitination Source: InterPro
  • regulation of protein stability Source: ZFIN
  • response to X-ray Source: ZFIN

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Mdm2 (EC:2.3.2.27)
Alternative name(s):
Double minute 2 protein
RING-type E3 ubiquitin transferase Mdm2Curated
p53-binding protein Mdm2
Gene namesi
Name:mdm2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-990415-153. mdm2.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001573351 – 445E3 ubiquitin-protein ligase Mdm2Add BLAST445

Proteomic databases

PRIDEiO42354.

Structurei

3D structure databases

ProteinModelPortaliO42354.
SMRiO42354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 100SWIBAdd BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 279ARF-bindingAdd BLAST90
Regioni222 – 306Region IIAdd BLAST85

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi160 – 166Nuclear localization signalSequence analysis7
Motifi171 – 183Nuclear export signalAdd BLAST13
Motifi420 – 427Nucleolar localization signalSequence analysis8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi141 – 145Poly-Arg5
Compositional biasi210 – 276Asp/Glu-rich (acidic)Add BLAST67

Sequence similaritiesi

Belongs to the MDM2/MDM4 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri274 – 303RanBP2-typePROSITE-ProRule annotationAdd BLAST30
Zinc fingeri392 – 433RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000293341.
HOVERGENiHBG013472.
InParanoidiO42354.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
InterProiView protein in InterPro
IPR028340. Mdm2.
IPR015459. MDM2_E3_ligase.
IPR016495. p53_neg-reg_MDM_2/4.
IPR003121. SWIB_MDM2_domain.
IPR001876. Znf_RanBP2.
IPR001841. Znf_RING.
PANTHERiPTHR13844:SF33. PTHR13844:SF33. 1 hit.
PfamiView protein in Pfam
PF02201. SWIB. 1 hit.
PIRSFiPIRSF500700. MDM2. 1 hit.
PIRSF006748. p53_MDM_2/4. 1 hit.
SUPFAMiSSF47592. SSF47592. 1 hit.
SSF90209. SSF90209. 1 hit.
PROSITEiView protein in PROSITE
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

O42354-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATESCLSSS QISKVDNEKL VRPKVQLKSL LEDAGADKDV FTMKEVMFYL
60 70 80 90 100
GKYIMSKELY DKQQQHIVHC GEDPLGAVLG VKSFSVKEPR ALFALINRNL
110 120 130 140 150
VTVKNPESQS TFSEPRSQSE PDRGPGDTDS DSRSSTSQQQ RRRRRSSDPE
160 170 180 190 200
SSSAEDESRE RRKRHKSDSF SLTFDDSLSW CVIGGLHRER GNSESSDANS
210 220 230 240 250
NSDVGISRSE GSEESEDSDS DSDNFSVEFE VESINSDAYS ENDVDSVPGE
260 270 280 290 300
NEIYEVTIFA EDEDSFDEDT EITEADYWKC PKCDQFNPPL PRHCKSCWTV
310 320 330 340 350
RADWLPETHS NWENLSRNTR TNPEDTSVTT TPNTTFEKKL SKPSSPLPET
360 370 380 390 400
DDGVDVPTPP LLRRGSSQEE TPELERFNSL EACLPATCLE PCVICQSRPK
410 420 430 440
NGCIVHGRTG HLMACYTCAK KLKNRNKLCP VCREPIQSVV LTYMS
Length:445
Mass (Da):49,950
Last modified:January 1, 1998 - v1
Checksum:i6FA8175A8A8E6261
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010255 mRNA. Translation: AAB64176.1.
AF356346 mRNA. Translation: AAM00198.1.
UniGeneiDr.75764.

Similar proteinsi

Entry informationi

Entry nameiMDM2_DANRE
AccessioniPrimary (citable) accession number: O42354
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: August 30, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families