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Protein

Growth/differentiation factor 8

Gene

mstnb

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts specifically as a negative regulator of skeletal muscle growth. May down-regulate muscle-specific transcription factors such as myod and myog.3 Publications

GO - Molecular functioni

  • cytokine activity Source: GO_Central
  • growth factor activity Source: UniProtKB
  • transforming growth factor beta receptor binding Source: GO_Central

GO - Biological processi

  • cell development Source: GO_Central
  • growth Source: InterPro
  • muscle organ development Source: ZFIN
  • negative regulation of growth Source: UniProtKB
  • negative regulation of growth rate Source: ZFIN
  • negative regulation of striated muscle tissue development Source: UniProtKB
  • positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
  • regulation of apoptotic process Source: GO_Central
  • regulation of MAPK cascade Source: GO_Central
  • skeletal muscle tissue development Source: ZFIN
  • SMAD protein signal transduction Source: GO_Central
  • transforming growth factor beta receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Growth/differentiation factor 8
Short name:
GDF-8
Alternative name(s):
Myostatin
Myostatin-1
Short name:
zfMSTN-1
Myostatin-B
Gene namesi
Name:mstnb
Synonyms:gdf8, mstn, mstn-1
ORF Names:si:ch211-3o3.2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 9

Organism-specific databases

ZFINiZDB-GENE-990415-165. mstnb.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • extracellular space Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Defects produce a giant phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000003397623 – 265Sequence analysisAdd BLAST243
ChainiPRO_0000033977266 – 374Growth/differentiation factor 8Add BLAST109

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi72N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi271 ↔ 281By similarity
Glycosylationi274N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi280 ↔ 339By similarity
Disulfide bondi308 ↔ 371By similarity
Disulfide bondi312 ↔ 373By similarity
Disulfide bondi338InterchainBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO42222.
PRIDEiO42222.

Expressioni

Tissue specificityi

Predominantly expressed in muscle. At hatching, expression is strongest in the skin epithelium, and is also found in the retina and brain. From day 28, expressed in skeletal muscle. In the adult, highest expression is seen in the gastrointestinal tract, brain, muscle, heart and testis. Also expressed in the adult pharynx, kidney, spleen, liver, gill, eyes, skin, swim bladder and ovary.3 Publications

Developmental stagei

Expressed both maternally and zygotically. Weakly expressed ubiquitously in early stage embryos. Present transiently in the blastula with levels dropping almost completely during gastrulation. Expression peaks during late somitogenesis, decreases at the end of somitogenesis, and then rises again at and after hatching. Strongly expressed in swimming larvae, juveniles and adults.5 Publications

Inductioni

Repressed in adult muscle, and stimulated in adult spleen, when fish are grown in overcrowded conditions.2 Publications

Gene expression databases

BgeeiENSDARG00000069133.
ExpressionAtlasiO42222. baseline and differential.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

GO - Molecular functioni

  • cytokine activity Source: GO_Central
  • growth factor activity Source: UniProtKB
  • transforming growth factor beta receptor binding Source: GO_Central

Protein-protein interaction databases

BioGridi663041. 2 interactors.
STRINGi7955.ENSDARP00000091159.

Structurei

3D structure databases

ProteinModelPortaliO42222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00760000119112.
HOGENOMiHOG000006566.
HOVERGENiHBG000217.
InParanoidiO42222.
KOiK05497.
OMAiFMEVKIS.
OrthoDBiEOG091G078V.
PhylomeDBiO42222.
TreeFamiTF318514.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR015616. GDF_8.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PTHR11848:SF150. PTHR11848:SF150. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O42222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFTQVLISL SVLIACGPVG YGDITAHQQP STATEESEQC STCEFRQHSK
60 70 80 90 100
LMRLHAIKSQ ILSKLRLKQA PNISRDVVKQ LLPKAPPLQQ LLDQYDVLGD
110 120 130 140 150
DSKDGAVEED DEHATTETIM TMATEPDPIV QVDRKPKCCF FSFSPKIQAN
160 170 180 190 200
RIVRAQLWVH LRPAEEATTV FLQISRLMPV KDGGRHRIRS LKIDVNAGVT
210 220 230 240 250
SWQSIDVKQV LTVWLKQPET NRGIEINAYD AKGNDLAVTS TETGEDGLLP
260 270 280 290 300
FMEVKISEGP KRIRRDSGLD CDENSSESRC CRYPLTVDFE DFGWDWIIAP
310 320 330 340 350
KRYKANYCSG ECDYMYLQKY PHTHLVNKAS PRGTAGPCCT PTKMSPINML
360 370
YFNGKEQIIY GKIPSMVVDR CGCS
Length:374
Mass (Da):42,075
Last modified:November 24, 2009 - v2
Checksum:i6E3B87A48E6E2172
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39Q → L in AAB86693 (PubMed:9356471).Curated1
Sequence conflicti39Q → L in AAP85526 (PubMed:14555747).Curated1
Sequence conflicti39Q → L in AAQ11222 (Ref. 3) Curated1
Sequence conflicti39Q → L in AAP13068 (Ref. 4) Curated1
Sequence conflicti204S → G in AAP85526 (PubMed:14555747).Curated1
Sequence conflicti330S → G in AAP85526 (PubMed:14555747).Curated1
Sequence conflicti367Missing in AAP85526 (PubMed:14555747).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019626 mRNA. Translation: AAB86693.1.
AY323521 Genomic DNA. Translation: AAP85526.1.
AF540956 mRNA. Translation: AAQ11222.1.
AY258034 mRNA. Translation: AAP13068.1.
AL672217 Genomic DNA. Translation: CAD43439.1.
BX323586 Genomic DNA. Translation: CAQ13470.1.
AJ318758 mRNA. Translation: CAC86466.1.
RefSeqiNP_571094.2. NM_131019.5.
UniGeneiDr.75782.

Genome annotation databases

EnsembliENSDART00000100386; ENSDARP00000091159; ENSDARG00000069133.
GeneIDi798441.
KEGGidre:798441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019626 mRNA. Translation: AAB86693.1.
AY323521 Genomic DNA. Translation: AAP85526.1.
AF540956 mRNA. Translation: AAQ11222.1.
AY258034 mRNA. Translation: AAP13068.1.
AL672217 Genomic DNA. Translation: CAD43439.1.
BX323586 Genomic DNA. Translation: CAQ13470.1.
AJ318758 mRNA. Translation: CAC86466.1.
RefSeqiNP_571094.2. NM_131019.5.
UniGeneiDr.75782.

3D structure databases

ProteinModelPortaliO42222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi663041. 2 interactors.
STRINGi7955.ENSDARP00000091159.

Proteomic databases

PaxDbiO42222.
PRIDEiO42222.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000100386; ENSDARP00000091159; ENSDARG00000069133.
GeneIDi798441.
KEGGidre:798441.

Organism-specific databases

CTDi798441.
ZFINiZDB-GENE-990415-165. mstnb.

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00760000119112.
HOGENOMiHOG000006566.
HOVERGENiHBG000217.
InParanoidiO42222.
KOiK05497.
OMAiFMEVKIS.
OrthoDBiEOG091G078V.
PhylomeDBiO42222.
TreeFamiTF318514.

Miscellaneous databases

PROiO42222.

Gene expression databases

BgeeiENSDARG00000069133.
ExpressionAtlasiO42222. baseline and differential.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR015616. GDF_8.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PTHR11848:SF150. PTHR11848:SF150. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGDF8_DANRE
AccessioniPrimary (citable) accession number: O42222
Secondary accession number(s): B0S643
, Q7T1K5, Q8JFS0, Q8UUR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 24, 2009
Last modified: November 30, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.