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O42138

- VM3AD_AGKCL

UniProt

O42138 - VM3AD_AGKCL

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Protein

Zinc metalloproteinase-disintegrin-like ACLD

Gene
N/A
Organism
Agkistrodon contortrix laticinctus (Broad-banded copperhead) (Agkistrodon mokasen laticinctus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Is a potent activator of prothrombin (F2). Does not elicit any hemorrhagic response. Barely inhibits collagen-induced platelet aggregation. Binds neither collagen, nor the jararhagin-monoclonal antibody MAJar3. Hydrolyzes the Aalpha-chain of fibrin and fibrinogen, without affecting the Bbeta- and gamma-chains. Is capable of triggering endothelial proinflammatory and procoagulant cell responses, but fails to trigger apoptosis. Induces von Willebrand factor release, and the expression of both ICAM1 and E-selectin (SELE) (without increase in VCAM1) in endothelial cells (HUVEC). Is also able to up-regulate the synthesis of the coagulation factor TF (F3). Enhances nitric oxide (NO) generation, prostacyclin production and interleukin-8 release By similarity.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Enzyme regulationi

Inhibited by EDTA and O-phenanthroline. Not inhibited by PMSF, benzamidine, irreversible serine-proteinase inhibitors and cysteine proteinase inhibitor E-64 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi202 – 2021Calcium 1 By similarity
Metal bindingi286 – 2861Calcium 1 By similarity
Metal bindingi335 – 3351Zinc; catalytic By similarity
Active sitei336 – 3361 By similarity
Metal bindingi339 – 3391Zinc; catalytic By similarity
Metal bindingi345 – 3451Zinc; catalytic By similarity
Metal bindingi390 – 3901Calcium 1; via carbonyl oxygen By similarity
Metal bindingi393 – 3931Calcium 1 By similarity
Metal bindingi405 – 4051Calcium 2; via carbonyl oxygen By similarity
Metal bindingi408 – 4081Calcium 2 By similarity
Metal bindingi410 – 4101Calcium 2; via carbonyl oxygen By similarity
Metal bindingi412 – 4121Calcium 2 By similarity
Metal bindingi415 – 4151Calcium 2 By similarity
Metal bindingi418 – 4181Calcium 2 By similarity

GO - Molecular functioni

  1. metalloendopeptidase activity Source: InterPro
  2. peptidase activator activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Blood coagulation cascade activating toxin, Cell adhesion impairing toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Prothrombin activator, Toxin

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase-disintegrin-like ACLD (EC:3.4.24.-)
Alternative name(s):
Snake venom metalloproteinase
Short name:
SVMP
VMP-III
Short name:
AclVMP-III
OrganismiAgkistrodon contortrix laticinctus (Broad-banded copperhead) (Agkistrodon mokasen laticinctus)
Taxonomic identifieri37195 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeAgkistrodon

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed predictionAdd
BLAST
Propeptidei21 – 189169 By similarityPRO_0000326416Add
BLAST
Chaini190 – 620431Zinc metalloproteinase-disintegrin-like ACLDPRO_0000326417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi259 – 2591N-linked (GlcNAc...) Reviewed prediction
Glycosylationi265 – 2651N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi310 ↔ 390 By similarity
Disulfide bondi350 ↔ 374 By similarity
Disulfide bondi352 ↔ 357 By similarity
Glycosylationi373 – 3731N-linked (GlcNAc...) Reviewed prediction
Glycosylationi396 – 3961N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi406 ↔ 435 By similarity
Disulfide bondi417 ↔ 430 By similarity
Disulfide bondi419 ↔ 425 By similarity
Disulfide bondi429 ↔ 452 By similarity
Disulfide bondi443 ↔ 449 By similarity
Disulfide bondi448 ↔ 474 By similarity
Disulfide bondi461 ↔ 481 By similarity
Disulfide bondi468 ↔ 500 By similarity
Disulfide bondi493 ↔ 505 By similarity
Glycosylationi502 – 5021N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi512 ↔ 562 By similarity
Disulfide bondi527 ↔ 573 By similarity
Glycosylationi536 – 5361N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi540 ↔ 550 By similarity
Disulfide bondi557 ↔ 599 By similarity
Disulfide bondi593 ↔ 604 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliO42138.
SMRiO42138. Positions 186-611.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini199 – 395197Peptidase M12BAdd
BLAST
Domaini403 – 48987DisintegrinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi467 – 4693D/ECD-tripeptide

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi490 – 620131Cys-richAdd
BLAST

Sequence similaritiesi

Contains 1 disintegrin domain.

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006978.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O42138-1 [UniParc]FASTAAdd to Basket

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MIQVLLVTLC LAVFPYQGSS IILESGNVND YEVVYPRKVT VLPKGAVQPK    50
YEDAMQYEFK VNGEPVVLHL EKNKQLFSKD YSETHYSPDG REITTYPLVE 100
DHCYYHGRIE NDADSTASIS ACNGLKGHFK LQGEMYLIDP LKLPDSEAHA 150
VFKYENVEKE DEAPKMCGVT QNWESYEPIK KASQLNLTPE QQAYLDAKKY 200
VEFVVVLDHG MYTKYKDNLD KIKTRIFEIV NTMNEMFIPL NIRVALICLE 250
IWSDKDKFNM TSAANVTSIS FRNWRATDLL KRKSHDNAQL LTVIDFDGPT 300
IGKAYMASMC DPKRSVGIIQ DHSTINLMMA VTMAHEMGHN LGMDHDEKYC 350
TCGAKSCVMA KALSRQPSKL FSNCSQEDYR KYLIKRRPKC ILNEPNGTDI 400
VSPPVCGNEL LEVGEECDCG SPTNCQNPCC DAATCKLTPG SQCADGVCCD 450
QCRFTRAGTE CRQAKDDCDM ADLCTGQSAE CPTDRFQRNG HPCLNDNGYC 500
YNRTCPTLKN QCIYFFGPNA AVAKDSCFKG NQKSNNHTYC RKENGKKIPC 550
APQDIKCGRL YCFRNLPGKK NICSVIYTPT DEDIGMVLPG TKCEDGKVCS 600
NGHCVDVNIA YKSTTGFSQI 620
Length:620
Mass (Da):69,512
Last modified:January 1, 1998 - v1
Checksum:i9016AFEB5AE0BB87
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961Y → H in ACV83929. 1 Publication
Sequence conflicti139 – 1391D → E in ACV83929. 1 Publication
Sequence conflicti268 – 2681S → L in ACV83929. 1 Publication
Sequence conflicti306 – 3061M → K in ACV83929. 1 Publication
Sequence conflicti463 – 4631Q → A in ACV83929. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U86634 mRNA. Translation: AAC18911.1.
GQ451435 mRNA. Translation: ACV83929.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U86634 mRNA. Translation: AAC18911.1 .
GQ451435 mRNA. Translation: ACV83929.1 .

3D structure databases

ProteinModelPortali O42138.
SMRi O42138. Positions 186-611.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG006978.

Family and domain databases

Gene3Di 3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProi IPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view ]
Pfami PF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view ]
PRINTSi PR00289. DISINTEGRIN.
SMARTi SM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view ]
SUPFAMi SSF57552. SSF57552. 1 hit.
PROSITEi PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of a cDNA sequence encoding a novel member of the snake venom metalloproteinase, disintegrin-like, cysteine-rich (MDC) protein family from Agkistrodon contortrix laticinctus."
    Selistre de Araujo H.S., de Souza D.H.F., Ownby C.L.
    Biochim. Biophys. Acta 1342:109-115(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  2. "Molecular cloning and characterization of cDNAs encoding metalloproteinases from snake venom glands."
    Jia Y., Perez J.C.
    Toxicon 55:462-469(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.

Entry informationi

Entry nameiVM3AD_AGKCL
AccessioniPrimary (citable) accession number: O42138
Secondary accession number(s): C9E1R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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