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Protein

DNA topoisomerase 2-beta

Gene

TOP2B

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.By similarity

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.PROSITE-ProRule annotation

Cofactori

Mg2+PROSITE-ProRule annotation, Mn2+PROSITE-ProRule annotation, Ca2+PROSITE-ProRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117ATPBy similarity1
Binding sitei146ATPBy similarity1
Metal bindingi487Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi567Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi567Magnesium 2PROSITE-ProRule annotation1
Metal bindingi569Magnesium 2PROSITE-ProRule annotation1
Sitei830Transition state stabilizerBy similarity1
Active sitei831O-(5'-phospho-DNA)-tyrosine intermediateBy similarity1
Sitei882Important for DNA bending; intercalates between base pairs of target DNABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 176ATPBy similarity3
Nucleotide bindingi187 – 194ATPBy similarity8
Nucleotide bindingi402 – 404ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 2-beta (EC:5.99.1.3)
Alternative name(s):
DNA topoisomerase II, beta isozyme
Gene namesi
Name:TOP2B
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • DNA topoisomerase complex (ATP-hydrolyzing) Source: GO_Central
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001453711 – 1627DNA topoisomerase 2-betaAdd BLAST1627

Proteomic databases

PaxDbiO42131.
PRIDEiO42131.

Interactioni

Subunit structurei

Homodimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei515Interaction with DNAPROSITE-ProRule annotation1
Sitei518Interaction with DNAPROSITE-ProRule annotation1
Sitei687Interaction with DNAPROSITE-ProRule annotation1
Sitei688Interaction with DNAPROSITE-ProRule annotation1
Sitei749Interaction with DNAPROSITE-ProRule annotation1
Sitei783Interaction with DNAPROSITE-ProRule annotation1
Sitei957Interaction with DNAPROSITE-ProRule annotation1

Protein-protein interaction databases

STRINGi9031.ENSGALP00000018410.

Structurei

3D structure databases

ProteinModelPortaliO42131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini481 – 598ToprimPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 370Interaction with DNABy similarity3
Regioni1016 – 1025Interaction with DNABy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 23Poly-GlyAdd BLAST19
Compositional biasi1265 – 1268Poly-Lys4
Compositional biasi1388 – 1391Poly-Asp4
Compositional biasi1393 – 1396Poly-Asn4

Sequence similaritiesi

Belongs to the type II topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0355. Eukaryota.
COG0187. LUCA.
COG0188. LUCA.
HOGENOMiHOG000216693.
HOVERGENiHBG052998.
InParanoidiO42131.
KOiK03164.
PhylomeDBiO42131.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR012542. DTHCT.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR028467. Top2b.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR031660. TOPRIM_C.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR10169:SF36. PTHR10169:SF36. 1 hit.
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF08070. DTHCT. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
PF16898. TOPRIM_C. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O42131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSGGGGGG GGGGGGGGGG SGGLTCVTLF DNQINASKKE ESESVNKNDT
60 70 80 90 100
SKKMSVERVY QKKTQLEHIL LRPDTYIGSV EPLTQLMWVY DEDVGMNCRE
110 120 130 140 150
VTFVPGLYKI FDEILVNAAD NKQRDKNMTC IKISIDPESN IISIWNNGKG
160 170 180 190 200
IPVVEHKVEK VYVPALIFGQ LLTSSNYDDD EKKVTGGRNG YGAKLCNIFS
210 220 230 240 250
TKFTVETACK EYKHSFKQTW MNNMMKTSEP KIKHFEGDDY TCITFQPDLS
260 270 280 290 300
KFKMENLDKD IVSLMTRRAY DLAGSCKGVK VMLNGKKLPV NGFRSYVDLY
310 320 330 340 350
VKDKLDETGV ALKVIHEVVN ERWDVCLTLS EKGFQQISFV NSIATTKGGR
360 370 380 390 400
HVDYVVDQVV GKLIEVVKKK NKAGVSVKPF QVKNHIWVFV NCLIENPSFD
410 420 430 440 450
SQTKENMTLQ PKSFGSKCQL SEKFFKAASN CGIIESILNW VKFKAQTQLN
460 470 480 490 500
KKCSSVKHSK IKGIPKLDDA NDAGGKHSLD CTLILTEGDS AKSLAVSGLG
510 520 530 540 550
VIGRDRYGVF PLRGKILNVR EASHKQIMEN AEINNIIKIV GLQYKKSYED
560 570 580 590 600
PESLKSLRYG KIMIMTDQDQ DGSHIKGLLI NFIHHNWPSL LKHGFLEEFI
610 620 630 640 650
TPIVKASKNK QELSFYSIPE FDEWKKHMEN HKAWKIKYYK GLGTSTAKEA
660 670 680 690 700
KEYFADMERH RILFRYAGPE DDAAITLAFS KKKIDDRKEW LTNFMEDRRQ
710 720 730 740 750
RRLHGLPEQF LYGTATKHLT YNDFINKELI LFSNSDNERS IPSLVDGLKP
760 770 780 790 800
GQRKVLFTCF KRNDKREVKV AQLAGSVAEM SAYHHGEQAL MMTIVNLAQN
810 820 830 840 850
FVGSNNVNLL QPIGQFGTRL HGGKDAASPR YIFTMLSPLA RLLFPSVDDN
860 870 880 890 900
LLKFLYDDNQ RVEPEWYIPI IPMVLVNGAE GIGTGWACKL PNYDTREIVN
910 920 930 940 950
NVRRMLDGLD PHPMLPNYKN FRGTIQELGQ NQYVVSGEIF VVDRNTVEIT
960 970 980 990 1000
ELPVRTWTQV YKEQVLEPML NGTEKTPALI SDYKEYHTDT TVKFVVKMTE
1010 1020 1030 1040 1050
EKLAQAEAAG LHKVFKLQTS LTCNSMVLFD HMGCLKKYET VQDILKEFFD
1060 1070 1080 1090 1100
LRLHYYSLRK EWLVGMLGAE STKLNNQARF ILEKIQGKIT IENRSKRDLI
1110 1120 1130 1140 1150
QMLVQRGYES DPVKAWKEAQ EKAAEEEDPQ NANDDASSAS GSTSGPDFNY
1160 1170 1180 1190 1200
ILNMSLWSLT KEKVEELIKH RDSKERELND LKRKSASDLW KEDLAAFVEE
1210 1220 1230 1240 1250
LEKVEAQERE DVLAGMVGKP IKGKVGKPKM KKLQLEETMP SPFGRRIVPQ
1260 1270 1280 1290 1300
ITSAMKADAS RKLLKKKKGD ADSVAIKMEF DEEFGGVQAE GGGDDTVNTA
1310 1320 1330 1340 1350
ASGTKTPKLK REKKEPGTRV RRAPSSTKSS AKKVKKRNPW SDDESKSESD
1360 1370 1380 1390 1400
LEESEPVIIP RDSLLRRAAA DRAKYTFDFS KEEDDAHDDD DANNNNDLDE
1410 1420 1430 1440 1450
LKVKASPVIN DREDEFVPSD SVEKDEYDFS PVKSKPSPEK MSQEKKNQDF
1460 1470 1480 1490 1500
GNIFSFPSYS QKTDDDTTKL DSDEEDSTPV FSSPFAPKQT EKMLSKTVAA
1510 1520 1530 1540 1550
KKAKVDVPPK PKRAPKAKKM ETVNSDSDSE FGIPKKTAAP KGKGRGAKKR
1560 1570 1580 1590 1600
KTSGSENEGE YNPGKKAPKS TPCKKSKKAA FDQDSDVEIF QSGFASETAP
1610 1620
KPRTGRARKE VKYFAESDED DDFDMFN
Length:1,627
Mass (Da):183,246
Last modified:January 1, 1998 - v1
Checksum:i8B651D10A2CAD34B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007446 mRNA. Translation: BAA22540.1.
RefSeqiNP_990413.1. NM_205082.1.
UniGeneiGga.30963.

Genome annotation databases

GeneIDi395966.
KEGGigga:395966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007446 mRNA. Translation: BAA22540.1.
RefSeqiNP_990413.1. NM_205082.1.
UniGeneiGga.30963.

3D structure databases

ProteinModelPortaliO42131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000018410.

Proteomic databases

PaxDbiO42131.
PRIDEiO42131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi395966.
KEGGigga:395966.

Organism-specific databases

CTDi7155.

Phylogenomic databases

eggNOGiKOG0355. Eukaryota.
COG0187. LUCA.
COG0188. LUCA.
HOGENOMiHOG000216693.
HOVERGENiHBG052998.
InParanoidiO42131.
KOiK03164.
PhylomeDBiO42131.

Miscellaneous databases

PROiO42131.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR012542. DTHCT.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR028467. Top2b.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR031660. TOPRIM_C.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR10169:SF36. PTHR10169:SF36. 1 hit.
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF08070. DTHCT. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
PF16898. TOPRIM_C. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOP2B_CHICK
AccessioniPrimary (citable) accession number: O42131
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.