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Protein

DNA topoisomerase 2-beta

Gene

TOP2B

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.By similarity

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.PROSITE-ProRule annotation

Cofactori

Mg2+PROSITE-ProRule annotation, Mn2+PROSITE-ProRule annotation, Ca2+PROSITE-ProRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117ATPBy similarity1
Binding sitei146ATPBy similarity1
Metal bindingi487Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi567Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi567Magnesium 2PROSITE-ProRule annotation1
Metal bindingi569Magnesium 2PROSITE-ProRule annotation1
Sitei830Transition state stabilizerBy similarity1
Active sitei831O-(5'-phospho-DNA)-tyrosine intermediateBy similarity1
Sitei882Important for DNA bending; intercalates between base pairs of target DNABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 176ATPBy similarity3
Nucleotide bindingi187 – 194ATPBy similarity8
Nucleotide bindingi402 – 404ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 2-beta (EC:5.99.1.3)
Alternative name(s):
DNA topoisomerase II, beta isozyme
Gene namesi
Name:TOP2B
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001453711 – 1627DNA topoisomerase 2-betaAdd BLAST1627

Proteomic databases

PaxDbiO42131
PRIDEiO42131

Interactioni

Subunit structurei

Homodimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei515Interaction with DNAPROSITE-ProRule annotation1
Sitei518Interaction with DNAPROSITE-ProRule annotation1
Sitei687Interaction with DNAPROSITE-ProRule annotation1
Sitei688Interaction with DNAPROSITE-ProRule annotation1
Sitei749Interaction with DNAPROSITE-ProRule annotation1
Sitei783Interaction with DNAPROSITE-ProRule annotation1
Sitei957Interaction with DNAPROSITE-ProRule annotation1

Protein-protein interaction databases

STRINGi9031.ENSGALP00000018410

Structurei

3D structure databases

ProteinModelPortaliO42131
SMRiO42131
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini481 – 598ToprimPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 370Interaction with DNABy similarity3
Regioni1016 – 1025Interaction with DNABy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 23Poly-GlyAdd BLAST19
Compositional biasi1265 – 1268Poly-Lys4
Compositional biasi1388 – 1391Poly-Asp4
Compositional biasi1393 – 1396Poly-Asn4

Sequence similaritiesi

Belongs to the type II topoisomerase family.Curated

Phylogenomic databases

eggNOGiKOG0355 Eukaryota
COG0187 LUCA
COG0188 LUCA
HOGENOMiHOG000216693
HOVERGENiHBG052998
InParanoidiO42131
KOiK03164
PhylomeDBiO42131

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd03365 TOPRIM_TopoIIA, 1 hit
Gene3Di1.10.268.10, 1 hit
3.30.230.10, 1 hit
3.30.565.10, 1 hit
3.40.50.670, 2 hits
3.90.199.10, 3 hits
InterProiView protein in InterPro
IPR012542 DTHCT
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR001241 Topo_IIA
IPR013760 Topo_IIA-like_dom_sf
IPR002205 Topo_IIA_A/C
IPR013758 Topo_IIA_A/C_ab
IPR013757 Topo_IIA_A_a_sf
IPR013759 Topo_IIA_B_C
IPR013506 Topo_IIA_bsu_dom2
IPR018522 TopoIIA_CS
IPR031660 TOPRIM_C
IPR006171 TOPRIM_domain
IPR034157 TOPRIM_TopoII
PANTHERiPTHR10169 PTHR10169, 1 hit
PfamiView protein in Pfam
PF00204 DNA_gyraseB, 1 hit
PF00521 DNA_topoisoIV, 1 hit
PF08070 DTHCT, 1 hit
PF02518 HATPase_c, 1 hit
PF01751 Toprim, 1 hit
PF16898 TOPRIM_C, 1 hit
SMARTiView protein in SMART
SM00433 TOP2c, 1 hit
SM00434 TOP4c, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
SSF56719 SSF56719, 1 hit
PROSITEiView protein in PROSITE
PS00177 TOPOISOMERASE_II, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

O42131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSGGGGGG GGGGGGGGGG SGGLTCVTLF DNQINASKKE ESESVNKNDT
60 70 80 90 100
SKKMSVERVY QKKTQLEHIL LRPDTYIGSV EPLTQLMWVY DEDVGMNCRE
110 120 130 140 150
VTFVPGLYKI FDEILVNAAD NKQRDKNMTC IKISIDPESN IISIWNNGKG
160 170 180 190 200
IPVVEHKVEK VYVPALIFGQ LLTSSNYDDD EKKVTGGRNG YGAKLCNIFS
210 220 230 240 250
TKFTVETACK EYKHSFKQTW MNNMMKTSEP KIKHFEGDDY TCITFQPDLS
260 270 280 290 300
KFKMENLDKD IVSLMTRRAY DLAGSCKGVK VMLNGKKLPV NGFRSYVDLY
310 320 330 340 350
VKDKLDETGV ALKVIHEVVN ERWDVCLTLS EKGFQQISFV NSIATTKGGR
360 370 380 390 400
HVDYVVDQVV GKLIEVVKKK NKAGVSVKPF QVKNHIWVFV NCLIENPSFD
410 420 430 440 450
SQTKENMTLQ PKSFGSKCQL SEKFFKAASN CGIIESILNW VKFKAQTQLN
460 470 480 490 500
KKCSSVKHSK IKGIPKLDDA NDAGGKHSLD CTLILTEGDS AKSLAVSGLG
510 520 530 540 550
VIGRDRYGVF PLRGKILNVR EASHKQIMEN AEINNIIKIV GLQYKKSYED
560 570 580 590 600
PESLKSLRYG KIMIMTDQDQ DGSHIKGLLI NFIHHNWPSL LKHGFLEEFI
610 620 630 640 650
TPIVKASKNK QELSFYSIPE FDEWKKHMEN HKAWKIKYYK GLGTSTAKEA
660 670 680 690 700
KEYFADMERH RILFRYAGPE DDAAITLAFS KKKIDDRKEW LTNFMEDRRQ
710 720 730 740 750
RRLHGLPEQF LYGTATKHLT YNDFINKELI LFSNSDNERS IPSLVDGLKP
760 770 780 790 800
GQRKVLFTCF KRNDKREVKV AQLAGSVAEM SAYHHGEQAL MMTIVNLAQN
810 820 830 840 850
FVGSNNVNLL QPIGQFGTRL HGGKDAASPR YIFTMLSPLA RLLFPSVDDN
860 870 880 890 900
LLKFLYDDNQ RVEPEWYIPI IPMVLVNGAE GIGTGWACKL PNYDTREIVN
910 920 930 940 950
NVRRMLDGLD PHPMLPNYKN FRGTIQELGQ NQYVVSGEIF VVDRNTVEIT
960 970 980 990 1000
ELPVRTWTQV YKEQVLEPML NGTEKTPALI SDYKEYHTDT TVKFVVKMTE
1010 1020 1030 1040 1050
EKLAQAEAAG LHKVFKLQTS LTCNSMVLFD HMGCLKKYET VQDILKEFFD
1060 1070 1080 1090 1100
LRLHYYSLRK EWLVGMLGAE STKLNNQARF ILEKIQGKIT IENRSKRDLI
1110 1120 1130 1140 1150
QMLVQRGYES DPVKAWKEAQ EKAAEEEDPQ NANDDASSAS GSTSGPDFNY
1160 1170 1180 1190 1200
ILNMSLWSLT KEKVEELIKH RDSKERELND LKRKSASDLW KEDLAAFVEE
1210 1220 1230 1240 1250
LEKVEAQERE DVLAGMVGKP IKGKVGKPKM KKLQLEETMP SPFGRRIVPQ
1260 1270 1280 1290 1300
ITSAMKADAS RKLLKKKKGD ADSVAIKMEF DEEFGGVQAE GGGDDTVNTA
1310 1320 1330 1340 1350
ASGTKTPKLK REKKEPGTRV RRAPSSTKSS AKKVKKRNPW SDDESKSESD
1360 1370 1380 1390 1400
LEESEPVIIP RDSLLRRAAA DRAKYTFDFS KEEDDAHDDD DANNNNDLDE
1410 1420 1430 1440 1450
LKVKASPVIN DREDEFVPSD SVEKDEYDFS PVKSKPSPEK MSQEKKNQDF
1460 1470 1480 1490 1500
GNIFSFPSYS QKTDDDTTKL DSDEEDSTPV FSSPFAPKQT EKMLSKTVAA
1510 1520 1530 1540 1550
KKAKVDVPPK PKRAPKAKKM ETVNSDSDSE FGIPKKTAAP KGKGRGAKKR
1560 1570 1580 1590 1600
KTSGSENEGE YNPGKKAPKS TPCKKSKKAA FDQDSDVEIF QSGFASETAP
1610 1620
KPRTGRARKE VKYFAESDED DDFDMFN
Length:1,627
Mass (Da):183,246
Last modified:January 1, 1998 - v1
Checksum:i8B651D10A2CAD34B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007446 mRNA Translation: BAA22540.1
RefSeqiNP_990413.1, NM_205082.1
UniGeneiGga.30963

Genome annotation databases

GeneIDi395966
KEGGigga:395966

Similar proteinsi

Entry informationi

Entry nameiTOP2B_CHICK
AccessioniPrimary (citable) accession number: O42131
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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