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Protein

DNA topoisomerase 2-alpha

Gene

TOP2A

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in the regulation of circadian rhythm.By similarity

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.PROSITE-ProRule annotation

Cofactori

Mg2+PROSITE-ProRule annotation, Mn2+PROSITE-ProRule annotation, Ca2+PROSITE-ProRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921ATPBy similarity
Binding sitei121 – 1211ATPBy similarity
Metal bindingi462 – 4621Magnesium 1; catalyticPROSITE-ProRule annotation
Metal bindingi542 – 5421Magnesium 1; catalyticPROSITE-ProRule annotation
Metal bindingi542 – 5421Magnesium 2PROSITE-ProRule annotation
Metal bindingi544 – 5441Magnesium 2PROSITE-ProRule annotation
Sitei805 – 8051Transition state stabilizerBy similarity
Active sitei806 – 8061O-(5'-phospho-DNA)-tyrosine intermediateBy similarity
Sitei857 – 8571Important for DNA bending; intercalates between base pairs of target DNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi149 – 1513ATPBy similarity
Nucleotide bindingi162 – 1698ATPBy similarity
Nucleotide bindingi377 – 3793ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Biological processi

Biological rhythms

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 2-alpha (EC:5.99.1.3)
Alternative name(s):
DNA topoisomerase II, alpha isozyme
Gene namesi
Name:TOP2A
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15531553DNA topoisomerase 2-alphaPRO_0000145367Add
BLAST

Proteomic databases

PaxDbiO42130.

Interactioni

Subunit structurei

Homodimer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei490 – 4901Interaction with DNAPROSITE-ProRule annotation
Sitei493 – 4931Interaction with DNAPROSITE-ProRule annotation
Sitei662 – 6621Interaction with DNAPROSITE-ProRule annotation
Sitei663 – 6631Interaction with DNAPROSITE-ProRule annotation
Sitei724 – 7241Interaction with DNAPROSITE-ProRule annotation
Sitei758 – 7581Interaction with DNAPROSITE-ProRule annotation
Sitei764 – 7641Interaction with DNAPROSITE-ProRule annotation
Sitei932 – 9321Interaction with DNAPROSITE-ProRule annotation

Protein-protein interaction databases

STRINGi9031.ENSGALP00000006239.

Structurei

3D structure databases

ProteinModelPortaliO42130.
SMRiO42130. Positions 30-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini456 – 573118ToprimPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni343 – 3453Interaction with DNABy similarity
Regioni991 – 100010Interaction with DNABy similarity

Sequence similaritiesi

Belongs to the type II topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0355. Eukaryota.
COG0187. LUCA.
COG0188. LUCA.
HOGENOMiHOG000216693.
HOVERGENiHBG052998.
InParanoidiO42130.
KOiK03164.
PhylomeDBiO42130.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR012542. DTHCT.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR028466. Top2a.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR031660. TOPRIM_C.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR10169:SF46. PTHR10169:SF46. 1 hit.
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF08070. DTHCT. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
PF16898. TOPRIM_C. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O42130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLDSPAPL RPLHDNPRLP KADGAQKRLS VERIYQKKTQ LEHILLRPDT
60 70 80 90 100
YIGSVETVTQ QMWVFDEDVG LNCRDVTFVP GLYKIFDEIL VNAADNKQRD
110 120 130 140 150
KSMSCIKVTI DPENNTISVW NNGKGIPVVE HKVEKVYVPA LIFGQLLTSS
160 170 180 190 200
NYDDDEKKVT GGRNGYGAKL CNIFSTKFTV ETACREYKKL FKQTWTDNMG
210 220 230 240 250
KAGEMTLKHF DGEDYTCVTF QPDLSKFKMT ILDKDIVALM SRRAYDIAGS
260 270 280 290 300
TKDVKVFLNG KRLPVKGFRS YVDLYLKDKV DETGNALKVI HEEVNSRWEV
310 320 330 340 350
CLTLSEKGFQ QVSFVNSIAT TKGGRHVDYV ADQIVTKLID VVKKKNKNGV
360 370 380 390 400
GVKPFQVKNH MWIFVNSLIE NPTFDSQTKE NMTLQAKSFG STCKLSEKFI
410 420 430 440 450
KGAVGCGIVE SILNWVKFKA QTQLNKKCSA VKHTKIKGVP KLDDANDAGS
460 470 480 490 500
KNSIDCTLIL TEGDSAKTLA VSGLGVVGRD KYGVFPLRGK MLNVREASHK
510 520 530 540 550
QIMENAEINN IIKIVGLQYK KNYEDRESLK SLRYGKIMIM TDQDQDGSHI
560 570 580 590 600
KGLLINFIHH NWPSLLRHNF LEEFITPIIK VSKNKEEIPF YSIPEFEEWK
610 620 630 640 650
SSTQNYNSWK IKYYKGLGTS TSKEAKEYFA DMARHRIGFK YSGPEDDAAI
660 670 680 690 700
TLAFSKKKVE ERKEWLTNFM EDRRQRNVHG LPEDYLYGKD TNYLTYNDFI
710 720 730 740 750
NKELVLFSNS DNERSIPSLV DGLKPGQRKV LFTCFKRNDK REVKGAQLAG
760 770 780 790 800
SVAEMSSYHH GEASLMMTII NLAQNFVGSN NLNLLQPIGQ FGTRLHGGKD
810 820 830 840 850
SASPRYIFTM LSPLARLLFP PVDDNVLRFL YDDNQRVEPE WYMPIIPMVL
860 870 880 890 900
INGAEGIGTG WSCKIPNFDI RETVNNIRCL LDGKEPLPML PSYKNFKGTI
910 920 930 940 950
DELGPNQYVI SGEVSILDST TIEITELPVR TWTQTYKEQV LEPMLNGTEK
960 970 980 990 1000
TPPLITDYKE YHTDTTVKFV VKMSEEKLAE AEAVGLHKVF KLQTNLTCNS
1010 1020 1030 1040 1050
MVLFDHVGFL KKYESPQDIL KEFFELRLRY YGLRKEWLIG MLGAESAKLN
1060 1070 1080 1090 1100
NQARFILEKI DGKIVIENKP KKELIQVLIQ RGYESDPVKA WKELQNKEEE
1110 1120 1130 1140 1150
EGDESGEESA AATGPDFNYL LNMPLWYLTK EKKDELCKQR DNKDKELEDL
1160 1170 1180 1190 1200
KHKSPSDLWK EDLAAFVEEL DAVEAKQMQD EMAGITGKPL KVKGGKQGGK
1210 1220 1230 1240 1250
QKVTKAQLAE VMPSPHGIRV VPRVTAEMKA EAEKRIKKKI KSEKNESDEK
1260 1270 1280 1290 1300
QEGNSSGDKE PSSLKQRLAQ KRKAEQGTKR QTTLPFKPIK KMKRNPWSDS
1310 1320 1330 1340 1350
ESDSESDDFE VPSKRERVVR QAAAKIKPMV NSDSDADLTS SDEDSEYQEN
1360 1370 1380 1390 1400
SEGNTDSDTT SKKKPPKAKA VPKEKKGKAP KEKPLPDAVP VRVQNVAAES
1410 1420 1430 1440 1450
ASQDPAAPPV SVPRAQAVPK KPAAAKKGST AKDNQPSIMD ILTKKKAAPK
1460 1470 1480 1490 1500
APRRAQREES PPSEATAAVA KKPGPPRGRK ATKRLTSSSD SDSDFGSRPS
1510 1520 1530 1540 1550
KSVAAKKSKR DDDDSYSIDL TADSPAAAAP RTRPGRLKKP VQYLESSDED

DMF
Length:1,553
Mass (Da):174,993
Last modified:May 4, 2001 - v2
Checksum:i3322CE95238F71B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007445 mRNA. Translation: BAA22539.2.
RefSeqiNP_990122.1. NM_204791.1.
UniGeneiGga.2010.

Genome annotation databases

GeneIDi395570.
KEGGigga:395570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007445 mRNA. Translation: BAA22539.2.
RefSeqiNP_990122.1. NM_204791.1.
UniGeneiGga.2010.

3D structure databases

ProteinModelPortaliO42130.
SMRiO42130. Positions 30-406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000006239.

Proteomic databases

PaxDbiO42130.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi395570.
KEGGigga:395570.

Organism-specific databases

CTDi7153.

Phylogenomic databases

eggNOGiKOG0355. Eukaryota.
COG0187. LUCA.
COG0188. LUCA.
HOGENOMiHOG000216693.
HOVERGENiHBG052998.
InParanoidiO42130.
KOiK03164.
PhylomeDBiO42130.

Miscellaneous databases

PROiO42130.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR012542. DTHCT.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR028466. Top2a.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR031660. TOPRIM_C.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR10169:SF46. PTHR10169:SF46. 1 hit.
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF08070. DTHCT. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
PF16898. TOPRIM_C. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOP2A_CHICK
AccessioniPrimary (citable) accession number: O42130
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 4, 2001
Last modified: June 8, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.