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O42099

- MK08B_CYPCA

UniProt

O42099 - MK08B_CYPCA

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Protein

Mitogen-activated protein kinase 8B

Gene

mapk8b

Organism
Cyprinus carpio (Common carp)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551ATPPROSITE-ProRule annotation
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 408ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 1195.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 8B (EC:2.7.11.24)
Short name:
MAP kinase 8B
Short name:
MAPK 8B
Alternative name(s):
Stress-activated protein kinase JNKb
c-Jun N-terminal kinase B
Gene namesi
Name:mapk8b
OrganismiCyprinus carpio (Common carp)Imported
Taxonomic identifieri7962 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCyprinus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Mitogen-activated protein kinase 8BPRO_0000186267Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831PhosphothreonineBy similarity
Modified residuei185 – 1851PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO42099.

Expressioni

Tissue specificityi

Expressed at high levels in the ovary and at lower levels in brain, gill, heart, spleen, liver, kidney, muscle, bladder and gut.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO42099.
SMRiO42099. Positions 10-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 321296Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi183 – 1853TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG014652.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O42099-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKNKREKEF YSVDVGDSTF TVLKRYQNLR PIGSGAQGIV CSAYDHNLER
60 70 80 90 100
NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKTLEE
110 120 130 140 150
FQDVYLVMEL MDANLCQVIQ MELDHERLSY LLYQMLCGTK HLHSAGIIHR
160 170 180 190 200
DLKPSNIVVK SDCTLKILDF GLARTAATGL LMTPYVVTRY YRAPEVILGM
210 220 230 240 250
GYQANVDVWS VGCIMAEMVR GSVLFPGSDH IDQWNKVIEQ LGTPSQEFMM
260 270 280 290 300
KLNQSVRTYV ENRPRYTGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK
310 320 330 340 350
MLVIDASKRI SVDEALQHPY INVWYDPAEV EAPPPLIIDK QLDEREHTVE
360 370 380 390 400
EWKELIFKEV LDWEERMKNG VIRGQPSPIG AAVINGSPQP SSSSSINDVS
410 420
SMSTEPTVAS DTDSSLEASA GPLSCCR
Length:427
Mass (Da):48,295
Last modified:January 1, 1998 - v1
Checksum:i9405E55B6F9E6D6F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB001744 mRNA. Translation: BAA22598.1.
PIRiJC5694.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB001744 mRNA. Translation: BAA22598.1 .
PIRi JC5694.

3D structure databases

ProteinModelPortali O42099.
SMRi O42099. Positions 10-364.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O42099.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG014652.

Enzyme and pathway databases

BRENDAi 2.7.11.24. 1195.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01772. JNKMAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure and expression of carp mitogen-activated protein kinases homologous to mammalian JNK/SAPK."
    Hashimoto H., Matsuo Y., Yokoyama Y., Toyohara H., Sakaguchi M.
    J. Biochem. 122:381-386(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Ovary.

Entry informationi

Entry nameiMK08B_CYPCA
AccessioniPrimary (citable) accession number: O42099
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3