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O42099 (MK08B_CYPCA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 8B

Short name=MAP kinase 8B
Short name=MAPK 8B
EC=2.7.11.24
Alternative name(s):
Stress-activated protein kinase JNKb
c-Jun N-terminal kinase B
Gene names
Name:mapk8b
OrganismCyprinus carpio (Common carp)
Taxonomic identifier7962 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCyprinus

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity By similarity. UniProtKB P45983

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity. UniProtKB P45983

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity. UniProtKB P45983

Tissue specificity

Expressed at high levels in the ovary and at lower levels in brain, gill, heart, spleen, liver, kidney, muscle, bladder and gut. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427Mitogen-activated protein kinase 8B
PRO_0000186267

Regions

Domain26 – 321296Protein kinase UniProtKB P53779
Nucleotide binding33 – 408ATP By similarity UniProtKB P53779
Motif183 – 1853TXY

Sites

Active site1511Proton acceptor By similarity UniProtKB P53779
Binding site551ATP By similarity UniProtKB P53779

Amino acid modifications

Modified residue1831Phosphothreonine By similarity UniProtKB P53779
Modified residue1851Phosphotyrosine By similarity UniProtKB P53779

Sequences

Sequence LengthMass (Da)Tools
O42099 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 9405E55B6F9E6D6F

FASTA42748,295
        10         20         30         40         50         60 
MNKNKREKEF YSVDVGDSTF TVLKRYQNLR PIGSGAQGIV CSAYDHNLER NVAIKKLSRP 

        70         80         90        100        110        120 
FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKTLEE FQDVYLVMEL MDANLCQVIQ 

       130        140        150        160        170        180 
MELDHERLSY LLYQMLCGTK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAATGL 

       190        200        210        220        230        240 
LMTPYVVTRY YRAPEVILGM GYQANVDVWS VGCIMAEMVR GSVLFPGSDH IDQWNKVIEQ 

       250        260        270        280        290        300 
LGTPSQEFMM KLNQSVRTYV ENRPRYTGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK 

       310        320        330        340        350        360 
MLVIDASKRI SVDEALQHPY INVWYDPAEV EAPPPLIIDK QLDEREHTVE EWKELIFKEV 

       370        380        390        400        410        420 
LDWEERMKNG VIRGQPSPIG AAVINGSPQP SSSSSINDVS SMSTEPTVAS DTDSSLEASA 


GPLSCCR 

« Hide

References

[1]"Structure and expression of carp mitogen-activated protein kinases homologous to mammalian JNK/SAPK."
Hashimoto H., Matsuo Y., Yokoyama Y., Toyohara H., Sakaguchi M.
J. Biochem. 122:381-386(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB001744 mRNA. Translation: BAA22598.1.
PIRJC5694.

3D structure databases

ProteinModelPortalO42099.
SMRO42099. Positions 10-364.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEO42099.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG014652.

Enzyme and pathway databases

BRENDA2.7.11.24. 1195.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01772. JNKMAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMK08B_CYPCA
AccessionPrimary (citable) accession number: O42099
Entry history
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families