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O42099

- MK08B_CYPCA

UniProt

O42099 - MK08B_CYPCA

Protein

Mitogen-activated protein kinase 8B

Gene

mapk8b

Organism
Cyprinus carpio (Common carp)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei55 – 551ATPPROSITE-ProRule annotation
    Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi33 – 408ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 1195.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 8B (EC:2.7.11.24)
    Short name:
    MAP kinase 8B
    Short name:
    MAPK 8B
    Alternative name(s):
    Stress-activated protein kinase JNKb
    c-Jun N-terminal kinase B
    Gene namesi
    Name:mapk8b
    OrganismiCyprinus carpio (Common carp)Imported
    Taxonomic identifieri7962 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCyprinus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427Mitogen-activated protein kinase 8BPRO_0000186267Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei183 – 1831PhosphothreonineBy similarity
    Modified residuei185 – 1851PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiO42099.

    Expressioni

    Tissue specificityi

    Expressed at high levels in the ovary and at lower levels in brain, gill, heart, spleen, liver, kidney, muscle, bladder and gut.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO42099.
    SMRiO42099. Positions 10-364.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 321296Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi183 – 1853TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG014652.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008351. MAPK_JNK.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01772. JNKMAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O42099-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKNKREKEF YSVDVGDSTF TVLKRYQNLR PIGSGAQGIV CSAYDHNLER    50
    NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKTLEE 100
    FQDVYLVMEL MDANLCQVIQ MELDHERLSY LLYQMLCGTK HLHSAGIIHR 150
    DLKPSNIVVK SDCTLKILDF GLARTAATGL LMTPYVVTRY YRAPEVILGM 200
    GYQANVDVWS VGCIMAEMVR GSVLFPGSDH IDQWNKVIEQ LGTPSQEFMM 250
    KLNQSVRTYV ENRPRYTGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK 300
    MLVIDASKRI SVDEALQHPY INVWYDPAEV EAPPPLIIDK QLDEREHTVE 350
    EWKELIFKEV LDWEERMKNG VIRGQPSPIG AAVINGSPQP SSSSSINDVS 400
    SMSTEPTVAS DTDSSLEASA GPLSCCR 427
    Length:427
    Mass (Da):48,295
    Last modified:January 1, 1998 - v1
    Checksum:i9405E55B6F9E6D6F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001744 mRNA. Translation: BAA22598.1.
    PIRiJC5694.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001744 mRNA. Translation: BAA22598.1 .
    PIRi JC5694.

    3D structure databases

    ProteinModelPortali O42099.
    SMRi O42099. Positions 10-364.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O42099.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG014652.

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 1195.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008351. MAPK_JNK.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01772. JNKMAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of carp mitogen-activated protein kinases homologous to mammalian JNK/SAPK."
      Hashimoto H., Matsuo Y., Yokoyama Y., Toyohara H., Sakaguchi M.
      J. Biochem. 122:381-386(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Ovary.

    Entry informationi

    Entry nameiMK08B_CYPCA
    AccessioniPrimary (citable) accession number: O42099
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 13, 2002
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3