O42053 (NCAP_HRSVB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoprotein
Short name=Protein N

Alternative name(s):
Nucleocapsid protein

Gene names

Name:

N

Organism

Human respiratory syncytial virus B (strain B1) [Reference proteome]

Taxonomic identifier

79692 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Pneumovirinae › Pneumovirus › Human respiratory syncytial virus › Human respiratory syncytial virus B

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Encapsidates the genome, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases By similarity.
Subunit structure	Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. In nucleocapsid, interacts with the protein P and thereby positions the polymerase on the template. Interacts with protein M2-1; this interaction allows the association of nucleocapsid with the matrix protein, supposely to shut down virus transcriptase activity and initiate assembly and budding By similarity.
Subcellular location	Virion. Host cytoplasm By similarity.
Sequence similarities	Belongs to the paramyxoviruses nucleocapsid family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host PKR by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Viral immunoevasion
Cellular component	Host cytoplasm Virion
Ligand	RNA-binding Viral nucleoprotein
Molecular function	Ribonucleoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	suppression by virus of host PKR activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW viral nucleocapsid Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	Nucleoprotein		PRO_0000365782

Sequences

	Sequence		Length	Mass (Da)
	O42053 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9A2B4E578E3B6D35	FASTA	391	43,411
	10 20 30 40 50 60 MALSKVKLND TLNKDQLLSS SKYTIQRSTG DNIDTPNYDV QKHLNKLCGM LLITEDANHK 70 80 90 100 110 120 FTGLIGMLYA MSRLGREDTI KILKDAGYHV KANGVDITTY RQDINGKEMK FEVLTLSSLT 130 140 150 160 170 180 SEIQVNIEIE SRKSYKKMLK EMGEVAPEYR HDSPDCGMII LCIAALVITK LAAGDRSGLT 190 200 210 220 230 240 AVIRRANNVL KNEIKRYKGL IPKDIANSFY EVFEKHPHLI DVFVHFGIAQ SSTRGGSRVE 250 260 270 280 290 300 GIFAGLFMNA YGSGQVMLRW GVLAKSVKNI MLGHASVQAE MEQVVEVYEY AQKLGGEAGF 310 320 330 340 350 360 YHILNNPKAS LLSLTQFPNF SSVVLGNAAG LGIMGEYRGT PRNQDLYDAA KAYAEQLKEN 370 380 390 GVINYSVLDL TAEELEAIKN QLNPKEDDVE L

References

[1]

"Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro: clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant." Karron R.A., Buonagurio D.A., Georgiu A.F., Whitehead S.S., Adamus J.E., Clements-Mann M.L., Harris D.O., Randolph V.B., Udem S.A., Murphy B.R., Sidhu M.S. Proc. Natl. Acad. Sci. U.S.A. 94:13961-13966(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF013254 Genomic RNA. Translation: AAB82431.1. AF013255 Genomic RNA. Translation: AAB82442.1.
RefSeq	NP_056858.1. NC_001781.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1489820.
Family and domain databases
InterPro	IPR004930. Pneumo_ncap. [Graphical view]
Pfam	PF03246. Pneumo_ncap. 1 hit. [Graphical view]
ProDom	PD006438. Pneumo_ncap. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

NCAP_HRSVB

Accession

Primary (citable) accession number: O42053

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program