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O41965

- DEN_MHV68

UniProt

O41965 - DEN_MHV68

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Protein

Deneddylase

Gene
ORF64
Organism
Murid herpesvirus 4 (MuHV-4) (Murine gammaherpesvirus 68)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei20 – 201Important for catalytic activity By similarity
Active sitei33 – 331 By similarity
Active sitei163 – 1631 By similarity
Active sitei165 – 1651 By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Tegument protein VP1-2
Tegument protein VP1/2
Gene namesi
ORF Names:ORF64
OrganismiMurid herpesvirus 4 (MuHV-4) (Murine gammaherpesvirus 68)
Taxonomic identifieri33708 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiApodemus sylvaticus (European woodmouse) [TaxID: 10129]

Subcellular locationi

Virion tegument. Host cytoplasm. Host nucleus By similarity
Note: Tightly associated with the capsid By similarity.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24572457DeneddylasePRO_0000406915Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Protein-protein interaction databases

DIPiDIP-47224N.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 224212Peptidase C76Add
BLAST
Repeati426 – 44924LRR 1Add
BLAST
Repeati1017 – 104226LRR 2Add
BLAST
Repeati1254 – 127724LRR 3Add
BLAST
Repeati1662 – 168524LRR 4Add
BLAST
Repeati1699 – 172123LRR 5Add
BLAST
Repeati1740 – 176324LRR 6Add
BLAST
Repeati1801 – 182727LRR 7Add
BLAST
Repeati2046 – 207126LRR 8Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Leucine-rich repeat, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O41965-1 [UniParc]FASTAAdd to Basket

« Hide

MALPASLAGF RIEGTASTNQ ADCKFGENAG AQCLSNCIIY LMSSYFNNEA     50
PITETHDLNK VLKFGAELDS NLRKLGLLSP GQYAQLDHVP CYVQTRKWSG 100
FIYTSAEMFG LLGMPADISD SCITSLRDLL TANYSNTIQY ILYICGQKSG 150
AIIIQGGRYT MFDPHCLKDI PESPAHVLST SDPDALIKYV GGVSREYTAC 200
FLYFVPGHIS PKNYIMSHYK VISFSDLHGS KIILEEYDLP TTNQSFCSSP 250
QASSSPDPEV GSLLKYMSKA KRKRYPMSCG EEWTDANKKR KESGRTPPEK 300
MTHPLPSPDI IDLTMDDDVI DLTGDDDMED ESEGDREAEP DRSGTSLPSV 350
TLPNLAAVDQ LLNSLTLPGH VPSFPALVDT DTGESYRLTR ALHQLKNVLQ 400
QVLEIGVVSD SDYTPTEALN VLNYLMAWSK QLQIKNDDIK LLINSNLQIE 450
KLFTLLKNNL ISDPNLADHV QAKVCACLPA MHANRATDLQ KILLHCKNLT 500
RALEISKSSL DIKDIITTFT ESFPQDFFCV CSVEEANNLV STVQDLKRVV 550
SNNMALTSEQ DARFKALMLS VLNNTDPPAS LGPIYLETET RTPLLSSAIQ 600
EAVKAIEKDT VETLSELISN IPSENAIETT FVPPVRTLLK NVTTLLTVIN 650
ACVERAEIRT PEIDSSQQQL SYIGRELSKI IDETWPERAF REPVHVLDIF 700
QKTSSHLNDL KKKMADSESL DKILSEINQT LKAIQDKAAS PALINTLSDY 750
IKNATVLAQA SDPRLVEIQS QVTTLTTSTS YIESLLQKIN IRTLPEVIPQ 800
LQAATKTEQG QLSLAAMNLS LIQITNSLIN EALSSIHARS HNHLSNTFFN 850
SLNSLMGLAD IPGREDLIKT MESIMAVQEE LVDCDDMECV EKGLTTFKYI 900
KSSIRQYKFD KSFKTKIYLI ITSEVRDLTK IKTDKQLEAW KQDVADFTPA 950
SIDDLNLFLD KAPTKTARSY ATRQLKHFRD DLIKEQEMET ETTPVPSTAE 1000
IERAIDLKIK ATWDKILTNL RDLTFHHIAP GDWQVLLTVF NDHKSALFTK 1050
MGGELLKALQ GLTAYVDSIL TPLLASQLPQ GQRYVAPNSD WVETFDQNVK 1100
YYLRTFHLPR VSEQLDDLER KTLLLTKLVK FTDLSQSLIG THLEKDWSIY 1150
QKLFNSLLTV YNDHLIKTKT EVHAFLDKIA SDPLLEPQAH PDLQKITQLF 1200
TEQEIIEINT LPDIFKESIK NNEKHYIASY QTEMKVFTSM VDAALAKKTQ 1250
STTEYNTHLL KIVNNMLVQA PPYAASHPIS SDAISYITSL VRDKHLLEKL 1300
SYAESLKNFN WLSRLITIIL TNCHPSHKQH LQTLLDEILS REQTLTPLVA 1350
LEDNANQSPT ERTLQAALTT LNVERVLGRA TTFQKWKSQL QELEEAVKTT 1400
TQVSLLIQTI SSLHDKTVTE MDPTILSSHS QALADKLKEL LALKPSLDET 1450
TMSLFHGMKA YAQFKHYFVQ HYVITQPKIF DAYPLSHHGT VSSSGGHQAT 1500
KFNPLMRLKA FSMVTDVKKM SVWREINTTV DPTGHTFIPA PPTPAMPPIH 1550
YNVLFSSFLQ AEAINLALNS NQPPTKKFGL LPGLMDARVG VQGAMLLDNQ 1600
WNDISTNSAK LLDHYVRSEL TPNSLTNSQF AAMTVFAHAM AMVTPHINST 1650
RATIFPSKAI VLNQLQFLKL CLTMWPKFSG GLLRAPSFER VVQLARATLP 1700
TLLLSAPRNT LNHFLANNYR PTDTLPNTEA LLFYPNQHPL VNLEKLLLTS 1750
SPFHALSTSV LNTRISMLVW GILSLSEAVL QQLWDSLYQE SATFTTYIDL 1800
LRHLSAMNHK NSTLTTSTSL PQNNGPVVYS YGHTAGTTVA TLEGSHPLDD 1850
GGQNIPMTLF EFVIFAIILK LKFHVFYTQE KALVNTQLGP LHLITHALDG 1900
TGDTEPFKTY ISIPPQKYNG LGNLQQFCSQ DEIQIFQRQH EWLVGVTKQT 1950
SFSNEDLFIV LASADNKVLS VHTFNPPINS LESETPEIVA APIQESWPKE 2000
ITTVSFWDKP ALEKSPQELI TEVSLVAEIF SGSAIFNTFP PSYKMVSHTP 2050
SLHVETHQLE NLSITEGTPP TSPPLPDSTT QDHMEEPDNK QAKPPYQMTS 2100
PMKENTSTSG RPARSPSPSP PVLTPIKPII PIPQATPTMP ILSPFTPRLL 2150
PAAVKKHQNG AVWGHSGSLP PTHIQSSTPG PAQNTRDSGR RQIVSPVITI 2200
LPGTKAGADS AGQNTSHKDI SAYSPPPASK KTDRPSDTHV TAPLFSKSKL 2250
VTPRPAAKTD TGTFGPLLGH EKPPVTDLTA PVEPGHPSKV SPIIHLKPSN 2300
TGDRDPHPIS DDEDSKQPPV PDTSRDKAQS RWKTPKQRPQ NIFPPPKHED 2350
DVPVTAPQPQ GRKILVGGRQ LPSLVYNPPT LRDIKTGMSD DKNPEPCVKE 2400
NPPGVTHDPP LRIQHMEQTV NSSKYNVLLF IEKIIKSVHD HSSYMLSTLK 2450
RIKQLYI 2457
Length:2,457
Mass (Da):273,538
Last modified:January 1, 1998 - v1
Checksum:i6AE1D5E1821BAAD1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97553 Genomic DNA. Translation: AAB66417.1.
AF105037 Genomic DNA. Translation: AAF19328.1.
RefSeqiNP_044902.1. NC_001826.2.

Genome annotation databases

GeneIDi1497174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97553 Genomic DNA. Translation: AAB66417.1 .
AF105037 Genomic DNA. Translation: AAF19328.1 .
RefSeqi NP_044902.1. NC_001826.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47224N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1497174.

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence and genomic analysis of murine gammaherpesvirus 68."
    Virgin H.W. IV, Latreille P., Wamsley P., Hallsworth K., Weck K.E., Dal Canto A.J., Speck S.H.
    J. Virol. 71:5894-5904(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G2.4 and WUMS.
  2. "A functional ubiquitin-specific protease embedded in the large tegument protein (ORF64) of murine gammaherpesvirus 68 is active during the course of infection."
    Gredmark S., Schlieker C., Quesada V., Spooner E., Ploegh H.L.
    J. Virol. 81:10300-10309(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDEN_MHV68
AccessioniPrimary (citable) accession number: O41965
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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