Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O41965

- DEN_MHV68

UniProt

O41965 - DEN_MHV68

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Deneddylase

Gene

ORF64

Organism
Murid herpesvirus 4 (MuHV-4) (Murine gammaherpesvirus 68)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei20 – 201Important for catalytic activityPROSITE-ProRule annotation
Active sitei33 – 331PROSITE-ProRule annotation
Active sitei163 – 1631PROSITE-ProRule annotation
Active sitei165 – 1651PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Tegument protein VP1-2
Tegument protein VP1/2
Gene namesi
ORF Names:ORF64
OrganismiMurid herpesvirus 4 (MuHV-4) (Murine gammaherpesvirus 68)
Taxonomic identifieri33708 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiApodemus sylvaticus (European woodmouse) [TaxID: 10129]

Subcellular locationi

Virion tegument. Host cytoplasm. Host nucleus By similarity
Note: Tightly associated with the capsid.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24572457DeneddylasePRO_0000406915Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

Protein-protein interaction databases

DIPiDIP-47224N.
IntActiO41965. 1 interaction.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 224212Peptidase C76PROSITE-ProRule annotationAdd
BLAST
Repeati426 – 44924LRR 1Add
BLAST
Repeati1017 – 104226LRR 2Add
BLAST
Repeati1254 – 127724LRR 3Add
BLAST
Repeati1662 – 168524LRR 4Add
BLAST
Repeati1699 – 172123LRR 5Add
BLAST
Repeati1740 – 176324LRR 6Add
BLAST
Repeati1801 – 182727LRR 7Add
BLAST
Repeati2046 – 207126LRR 8Add
BLAST

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 8 LRR (leucine-rich) repeats.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Leucine-rich repeat, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O41965-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALPASLAGF RIEGTASTNQ ADCKFGENAG AQCLSNCIIY LMSSYFNNEA
60 70 80 90 100
PITETHDLNK VLKFGAELDS NLRKLGLLSP GQYAQLDHVP CYVQTRKWSG
110 120 130 140 150
FIYTSAEMFG LLGMPADISD SCITSLRDLL TANYSNTIQY ILYICGQKSG
160 170 180 190 200
AIIIQGGRYT MFDPHCLKDI PESPAHVLST SDPDALIKYV GGVSREYTAC
210 220 230 240 250
FLYFVPGHIS PKNYIMSHYK VISFSDLHGS KIILEEYDLP TTNQSFCSSP
260 270 280 290 300
QASSSPDPEV GSLLKYMSKA KRKRYPMSCG EEWTDANKKR KESGRTPPEK
310 320 330 340 350
MTHPLPSPDI IDLTMDDDVI DLTGDDDMED ESEGDREAEP DRSGTSLPSV
360 370 380 390 400
TLPNLAAVDQ LLNSLTLPGH VPSFPALVDT DTGESYRLTR ALHQLKNVLQ
410 420 430 440 450
QVLEIGVVSD SDYTPTEALN VLNYLMAWSK QLQIKNDDIK LLINSNLQIE
460 470 480 490 500
KLFTLLKNNL ISDPNLADHV QAKVCACLPA MHANRATDLQ KILLHCKNLT
510 520 530 540 550
RALEISKSSL DIKDIITTFT ESFPQDFFCV CSVEEANNLV STVQDLKRVV
560 570 580 590 600
SNNMALTSEQ DARFKALMLS VLNNTDPPAS LGPIYLETET RTPLLSSAIQ
610 620 630 640 650
EAVKAIEKDT VETLSELISN IPSENAIETT FVPPVRTLLK NVTTLLTVIN
660 670 680 690 700
ACVERAEIRT PEIDSSQQQL SYIGRELSKI IDETWPERAF REPVHVLDIF
710 720 730 740 750
QKTSSHLNDL KKKMADSESL DKILSEINQT LKAIQDKAAS PALINTLSDY
760 770 780 790 800
IKNATVLAQA SDPRLVEIQS QVTTLTTSTS YIESLLQKIN IRTLPEVIPQ
810 820 830 840 850
LQAATKTEQG QLSLAAMNLS LIQITNSLIN EALSSIHARS HNHLSNTFFN
860 870 880 890 900
SLNSLMGLAD IPGREDLIKT MESIMAVQEE LVDCDDMECV EKGLTTFKYI
910 920 930 940 950
KSSIRQYKFD KSFKTKIYLI ITSEVRDLTK IKTDKQLEAW KQDVADFTPA
960 970 980 990 1000
SIDDLNLFLD KAPTKTARSY ATRQLKHFRD DLIKEQEMET ETTPVPSTAE
1010 1020 1030 1040 1050
IERAIDLKIK ATWDKILTNL RDLTFHHIAP GDWQVLLTVF NDHKSALFTK
1060 1070 1080 1090 1100
MGGELLKALQ GLTAYVDSIL TPLLASQLPQ GQRYVAPNSD WVETFDQNVK
1110 1120 1130 1140 1150
YYLRTFHLPR VSEQLDDLER KTLLLTKLVK FTDLSQSLIG THLEKDWSIY
1160 1170 1180 1190 1200
QKLFNSLLTV YNDHLIKTKT EVHAFLDKIA SDPLLEPQAH PDLQKITQLF
1210 1220 1230 1240 1250
TEQEIIEINT LPDIFKESIK NNEKHYIASY QTEMKVFTSM VDAALAKKTQ
1260 1270 1280 1290 1300
STTEYNTHLL KIVNNMLVQA PPYAASHPIS SDAISYITSL VRDKHLLEKL
1310 1320 1330 1340 1350
SYAESLKNFN WLSRLITIIL TNCHPSHKQH LQTLLDEILS REQTLTPLVA
1360 1370 1380 1390 1400
LEDNANQSPT ERTLQAALTT LNVERVLGRA TTFQKWKSQL QELEEAVKTT
1410 1420 1430 1440 1450
TQVSLLIQTI SSLHDKTVTE MDPTILSSHS QALADKLKEL LALKPSLDET
1460 1470 1480 1490 1500
TMSLFHGMKA YAQFKHYFVQ HYVITQPKIF DAYPLSHHGT VSSSGGHQAT
1510 1520 1530 1540 1550
KFNPLMRLKA FSMVTDVKKM SVWREINTTV DPTGHTFIPA PPTPAMPPIH
1560 1570 1580 1590 1600
YNVLFSSFLQ AEAINLALNS NQPPTKKFGL LPGLMDARVG VQGAMLLDNQ
1610 1620 1630 1640 1650
WNDISTNSAK LLDHYVRSEL TPNSLTNSQF AAMTVFAHAM AMVTPHINST
1660 1670 1680 1690 1700
RATIFPSKAI VLNQLQFLKL CLTMWPKFSG GLLRAPSFER VVQLARATLP
1710 1720 1730 1740 1750
TLLLSAPRNT LNHFLANNYR PTDTLPNTEA LLFYPNQHPL VNLEKLLLTS
1760 1770 1780 1790 1800
SPFHALSTSV LNTRISMLVW GILSLSEAVL QQLWDSLYQE SATFTTYIDL
1810 1820 1830 1840 1850
LRHLSAMNHK NSTLTTSTSL PQNNGPVVYS YGHTAGTTVA TLEGSHPLDD
1860 1870 1880 1890 1900
GGQNIPMTLF EFVIFAIILK LKFHVFYTQE KALVNTQLGP LHLITHALDG
1910 1920 1930 1940 1950
TGDTEPFKTY ISIPPQKYNG LGNLQQFCSQ DEIQIFQRQH EWLVGVTKQT
1960 1970 1980 1990 2000
SFSNEDLFIV LASADNKVLS VHTFNPPINS LESETPEIVA APIQESWPKE
2010 2020 2030 2040 2050
ITTVSFWDKP ALEKSPQELI TEVSLVAEIF SGSAIFNTFP PSYKMVSHTP
2060 2070 2080 2090 2100
SLHVETHQLE NLSITEGTPP TSPPLPDSTT QDHMEEPDNK QAKPPYQMTS
2110 2120 2130 2140 2150
PMKENTSTSG RPARSPSPSP PVLTPIKPII PIPQATPTMP ILSPFTPRLL
2160 2170 2180 2190 2200
PAAVKKHQNG AVWGHSGSLP PTHIQSSTPG PAQNTRDSGR RQIVSPVITI
2210 2220 2230 2240 2250
LPGTKAGADS AGQNTSHKDI SAYSPPPASK KTDRPSDTHV TAPLFSKSKL
2260 2270 2280 2290 2300
VTPRPAAKTD TGTFGPLLGH EKPPVTDLTA PVEPGHPSKV SPIIHLKPSN
2310 2320 2330 2340 2350
TGDRDPHPIS DDEDSKQPPV PDTSRDKAQS RWKTPKQRPQ NIFPPPKHED
2360 2370 2380 2390 2400
DVPVTAPQPQ GRKILVGGRQ LPSLVYNPPT LRDIKTGMSD DKNPEPCVKE
2410 2420 2430 2440 2450
NPPGVTHDPP LRIQHMEQTV NSSKYNVLLF IEKIIKSVHD HSSYMLSTLK

RIKQLYI
Length:2,457
Mass (Da):273,538
Last modified:January 1, 1998 - v1
Checksum:i6AE1D5E1821BAAD1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97553 Genomic DNA. Translation: AAB66417.1.
AF105037 Genomic DNA. Translation: AAF19328.1.
RefSeqiNP_044902.1. NC_001826.2.

Genome annotation databases

GeneIDi1497174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97553 Genomic DNA. Translation: AAB66417.1 .
AF105037 Genomic DNA. Translation: AAF19328.1 .
RefSeqi NP_044902.1. NC_001826.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47224N.
IntActi O41965. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1497174.

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence and genomic analysis of murine gammaherpesvirus 68."
    Virgin H.W. IV, Latreille P., Wamsley P., Hallsworth K., Weck K.E., Dal Canto A.J., Speck S.H.
    J. Virol. 71:5894-5904(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G2.4 and WUMS.
  2. "A functional ubiquitin-specific protease embedded in the large tegument protein (ORF64) of murine gammaherpesvirus 68 is active during the course of infection."
    Gredmark S., Schlieker C., Quesada V., Spooner E., Ploegh H.L.
    J. Virol. 81:10300-10309(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDEN_MHV68
AccessioniPrimary (citable) accession number: O41965
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3