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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

GAMMAHV.ORF61

Organism
Murid herpesvirus 4 (MuHV-4) (Murine gammaherpesvirus 68)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation
Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei180SubstrateUniRule annotation1
Sitei196Important for hydrogen atom transferUniRule annotation1
Binding sitei226Substrate; via amide nitrogenUniRule annotation1
Active sitei398Proton acceptorUniRule annotation1
Active sitei400Cysteine radical intermediateUniRule annotation1
Active sitei402Proton acceptorUniRule annotation1
Sitei414Important for hydrogen atom transferUniRule annotation1
Sitei721Important for electron transferUniRule annotation1
Sitei722Important for electron transferUniRule annotation1
Sitei771Interacts with thioredoxin/glutaredoxinUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotation
Biological processDNA replicationUniRule annotation
LigandATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Short name:
R1UniRule annotation
Alternative name(s):
Ribonucleotide reductase large subunitUniRule annotation
Gene namesi
Name:GAMMAHV.ORF61Imported
Synonyms:61Imported, RIR1UniRule annotation
OrganismiMurid herpesvirus 4 (MuHV-4) (Murine gammaherpesvirus 68)Imported
Taxonomic identifieri33708 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiApodemus sylvaticus (European woodmouse) [TaxID: 10129]
Proteomesi
  • UP000099649 Componenti: Genome
  • UP000175018 Componenti: Genome

PTM / Processingi

Keywords - PTMi

Disulfide bondUniRule annotation

Expressioni

Keywords - Developmental stagei

Early proteinUniRule annotation

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Protein-protein interaction databases

DIPiDIP-47191N.
IntActiO41962. 1 interactor.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini568 – 590RIBORED_LARGEInterPro annotationAdd BLAST23

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 196Substrate bindingUniRule annotation2
Regioni398 – 402Substrate bindingUniRule annotation5
Regioni590 – 594Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase large chain family.UniRule annotation

Phylogenomic databases

KOiK10807.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiView protein in InterPro
IPR034717. HSV_RIR1.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
PfamiView protein in Pfam
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiView protein in PROSITE
PS00089. RIBORED_LARGE. 1 hit.

Sequencei

Sequence statusi: Complete.

O41962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATQTMDVEP VQTVNAIDAA IAELKLMAGH SHEANVLAGR LLHFKMREVC
60 70 80 90 100
TQTVAQFLDA FSSVLDEGLC AFIGNYSQSL DEMVQRYVSG GFAAVKENGY
110 120 130 140 150
LAAARFYDTY VLRNDVKYES VPHMFMRISA FCAYHCIQNE CLFKTLKHME
160 170 180 190 200
LDRGNVVENA MDLITYFFEM IASQQVCCAT PVMRSAGLRD ANLSSCFIFA
210 220 230 240 250
PALDSEEKTV DAVFSELTKL LACKSGVGMD LSTYSHGKNL HAVLKALDGQ
260 270 280 290 300
ITYFNDHNIR PVSVAAYMEI WHTNIMDFLT AKLPENPERC GNLFQGVCIP
310 320 330 340 350
EIFFRTYKND PESNWYLFDP KKAEVLTNTY GYMFSIAYAG LVEQGAYEAV
360 370 380 390 400
VPIKSVMFAL VSCIIKTGGP YILNKKAINR HDWHEWSTHQ YRAINCANLC
410 420 430 440 450
AEVIQYPGEN VSTCNLANIC LPKCLEDDKF VSGKSCLLTY EREFSIERLE
460 470 480 490 500
RATEAAVFII NACIHGGTLP TQRARRGQSD RSMGIGVQGL ADTFAMMGLG
510 520 530 540 550
YFDAGSEILD QKIAETLYFN ALNTSINFVK IGKGEPYANY EQSKHSIGVL
560 570 580 590 600
HWHDWDMCSP MVYTRDEWED LARSCEVHGV YNSQFVAYMP TAGTGQITGY
610 620 630 640 650
SDSFYPFYSS MSSKVSNKEE ILRPNMTLMS RMSDSEVQTL RECNWDIAKL
660 670 680 690 700
PEPLLQKYRI FLSAFDYEPS LYVRRAMLRA PFIDQSQSMT LFLNEDYASS
710 720 730 740 750
ASNIKNLLMY GWECGLKTLM YYCRIRKTAT SNEFECVRRN SQQKYVNCAD
760 770 780
GEGAGGEEPS ENCIKAEGAT CSLSVGASCL HCQ
Length:783
Mass (Da):88,008
Last modified:January 1, 1998 - v1
Checksum:i13538196A4022C3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97553 Genomic DNA. Translation: AAB66451.1.
AF105037 Genomic DNA. Translation: AAF19325.1.
RefSeqiNP_044899.1. NC_001826.2.

Genome annotation databases

GeneIDi1497127.
KEGGivg:1497127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97553 Genomic DNA. Translation: AAB66451.1.
AF105037 Genomic DNA. Translation: AAF19325.1.
RefSeqiNP_044899.1. NC_001826.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47191N.
IntActiO41962. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1497127.
KEGGivg:1497127.

Phylogenomic databases

KOiK10807.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiView protein in InterPro
IPR034717. HSV_RIR1.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
PfamiView protein in Pfam
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiView protein in PROSITE
PS00089. RIBORED_LARGE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiO41962_MHV68
AccessioniPrimary (citable) accession number: O41962
Entry historyiIntegrated into UniProtKB/TrEMBL: January 1, 1998
Last sequence update: January 1, 1998
Last modified: June 7, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.