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Protein

Shutoff alkaline exonuclease

Gene

GAMMAHV.ORF37

Organism
Murid herpesvirus 4 (MuHV-4) (Murine gammaherpesvirus 68)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays a role in processing non linear or branched viral DNA intermediates in order to promote the production of mature packaged unit-length linear progeny viral DNA molecules. Exhibits endonuclease and exonuclease activities and accepts both double-stranded and single-stranded DNA as substrate. Exonuclease digestion of DNA is in the 5'-> 3' direction and the products are 5'-monophosphate nucleosides. Additionally, forms a recombinase with the major DNA-binding protein, which displays strand exchange activity. Also acts as a cytoplasmic RNA endonuclease that induces degradation of the majority of the cellular messenger RNAs during early lytic infection. The resulting inhibition of cellular protein synthesis serves to ensure maximal viral gene expression and evasion from host immune response. Internally cleaves host mRNAs which are then degraded by the cellular exonuclease XRN1. Bypasses therefore the regulatory steps of deadenylation and decapping normally required for XRN1 activation.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei182Required for functionUniRule annotation1
Sitei219Required for functionUniRule annotation1
Sitei242Required for functionUniRule annotation1
Sitei244Required for functionUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonucleaseUniRule annotation, ExonucleaseUniRule annotationImported, Hydrolase, Nuclease
Biological processHost-virus interactionUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Shutoff alkaline exonucleaseUniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
SOXUniRule annotation
Gene namesi
Name:GAMMAHV.ORF37Imported
Synonyms:37Imported
OrganismiMurid herpesvirus 4 (MuHV-4) (Murine gammaherpesvirus 68)Imported
Taxonomic identifieri33708 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiApodemus sylvaticus (European woodmouse) [TaxID: 10129]
Proteomesi
  • UP000099649 Componenti: Genome
  • UP000175018 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation
  • Host cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasmUniRule annotation, Host nucleusUniRule annotation

Interactioni

Subunit structurei

Forms a complex with the DNA polymerase, the DNA polymerase processivity factor, and the major DNA binding protein.UniRule annotation

Protein-protein interaction databases

DIPiDIP-47235N.
IntActiO41954. 5 interactors.

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae alkaline nuclease family.UniRule annotation

Family and domain databases

Gene3Di3.90.320.10. 1 hit.
HAMAPiMF_04009. HSV_AN. 1 hit.
InterProiView protein in InterPro
IPR011604. Exonuc_phg/RecB_C.
IPR001616. Herpes_alk_exo.
IPR011335. Restrct_endonuc-II-like.
IPR034720. Viral_alk_exo.
PfamiView protein in Pfam
PF01771. Herpes_alk_exo. 1 hit.
PRINTSiPR00924. ALKEXNUCLASE.
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

O41954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGSIILDFF EKDDLLEVLG NMARENQLES LATLNFSAFI RSPQVQELLA
60 70 80 90 100
TSKTSVRISD MRLTYYYFLF LRLNEYIGNT AIMGVFKDMM HLTDNSEVSA
110 120 130 140 150
VYAACRDVTP DIKYAVCQRI EALTRGQQDN ELWDILRDGM ISSSKFQWAV
160 170 180 190 200
KQHSINKKLF NPQPIKVNHY FAGPLAFGIR CEQTVKKILS ELIHPNLPRF
210 220 230 240 250
HDCGFLPSAI DGIFGVSLDT AFNVFTDSSG LVHFEPDSIV YEIKSRYKYQ
260 270 280 290 300
FSKSEFDGLA KKYLDLYKNP CEATFIKFIN CISRPAVEYV PHGKLPSESD
310 320 330 340 350
YLITHSMNWK TSNKRKRKIT DSHICLKKCL LHNMYQQSTV YILSDPSETS
360 370 380 390 400
GKITIKASFP IDVFVNPAHN YFYQVALQNM VVQDYIEFGQ GVYKRLGHQK
410 420 430 440 450
NFIASGFFRK RHFSDPAVCS IGNCGKLDTT DEIPVALIIT PVRIPSTVLH
460 470 480
EYLKKAIDFW NQCAEANFDH IPWGQLSDVA RKPITP
Length:486
Mass (Da):55,573
Last modified:January 1, 1998 - v1
Checksum:i5DC33CB77AB5AD23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97553 Genomic DNA. Translation: AAB66406.1.
AF105037 Genomic DNA. Translation: AAF19302.1.
RefSeqiNP_044875.1. NC_001826.2.

Genome annotation databases

GeneIDi1497163.
KEGGivg:1497163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97553 Genomic DNA. Translation: AAB66406.1.
AF105037 Genomic DNA. Translation: AAF19302.1.
RefSeqiNP_044875.1. NC_001826.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47235N.
IntActiO41954. 5 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1497163.
KEGGivg:1497163.

Family and domain databases

Gene3Di3.90.320.10. 1 hit.
HAMAPiMF_04009. HSV_AN. 1 hit.
InterProiView protein in InterPro
IPR011604. Exonuc_phg/RecB_C.
IPR001616. Herpes_alk_exo.
IPR011335. Restrct_endonuc-II-like.
IPR034720. Viral_alk_exo.
PfamiView protein in Pfam
PF01771. Herpes_alk_exo. 1 hit.
PRINTSiPR00924. ALKEXNUCLASE.
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiO41954_MHV68
AccessioniPrimary (citable) accession number: O41954
Entry historyiIntegrated into UniProtKB/TrEMBL: January 1, 1998
Last sequence update: January 1, 1998
Last modified: May 10, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.