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Protein

Microtubule-associated proteins 1A/1B light chain 3B

Gene

MAP1LC3B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-BTA-1632852. Macroautophagy.
R-BTA-5205685. Pink/Parkin Mediated Mitophagy.
R-BTA-8854214. TBC/RABGAPs.
R-BTA-8934903. Receptor Mediated Mitophagy.

Protein family/group databases

MEROPSiC74.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated proteins 1A/1B light chain 3B
Alternative name(s):
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
MAP1 light chain 3-like protein 2
MAP1A/MAP1B light chain 3 B
Short name:
MAP1A/MAP1B LC3 B
Microtubule-associated protein 1 light chain 3 beta
Gene namesi
Name:MAP1LC3B
Synonyms:MAP1ALC3, MAP1LC3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00004386502 – 120Microtubule-associated proteins 1A/1B light chain 3BAdd BLAST119
PropeptideiPRO_0000212361121 – 125Removed in mature form5

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphothreonine; by PKABy similarity1
Lipidationi120Phosphatidylethanolamine amidated glycineBy similarity1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.By similarity
Phosphorylation at Thr-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) (By similarity). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei120 – 121Cleavage; by ATG4BBy similarity2

Keywords - PTMi

Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiO41515.
PRIDEiO41515.

Expressioni

Gene expression databases

BgeeiENSBTAG00000011632.

Interactioni

Subunit structurei

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Interacts with FYCO1 (via C-terminus). Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. Interacts with ATG4B, MAPK15 and BNIP3. Interacts with MAPB1, KEAP1, PCM1, OFD1, CEP131, and TECPR2. Interacts with TBC1D5. Found in a complex with UBQLN1 and UBQLN2. Interacts with UBQLN4 (via STI1 1 and 2 domains). Interacts with UBQLN1 in the presence of UBQLN4. Interacts with ATG13. Interacts with RETREG1, RETREG2 and RETREG3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO41515. 1 interactor.
MINTiMINT-194225.
STRINGi9913.ENSBTAP00000015449.

Structurei

3D structure databases

ProteinModelPortaliO41515.
SMRiO41515.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiO41515.
KOiK10435.
OMAiIRDQHPT.
OrthoDBiEOG091G18ST.
TreeFamiTF312964.

Family and domain databases

CDDicd01611. GABARAP. 1 hit.
InterProiView protein in InterPro
IPR004241. Atg8-like.
IPR029071. Ubiquitin-like_domsf.
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiView protein in Pfam
PF02991. Atg8. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O41515-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT
60 70 80 90 100
KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPICEVYE
110 120
SEKDEDGFLY MVYASQETFG MKLSV
Length:125
Mass (Da):14,704
Last modified:January 23, 2007 - v4
Checksum:iBB141DEC7642E92F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4 – 5EK → DR AA sequence (PubMed:7908909).Curated2
Sequence conflicti6T → P AA sequence (PubMed:7908909).Curated1
Sequence conflicti12T → S AA sequence (PubMed:7908909).Curated1
Sequence conflicti14 – 16EQR → DDV AA sequence (PubMed:7908909).Curated3
Sequence conflicti18 – 21EDVR → KEVQ AA sequence (PubMed:7908909).Curated4
Sequence conflicti111M → T in AAB72082 (PubMed:9557703).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY570553 mRNA. Translation: AAS78585.1.
BC102891 mRNA. Translation: AAI02892.1.
U80885 Genomic RNA. Translation: AAB72082.1. Sequence problems.
RefSeqiNP_001001169.1. NM_001001169.1.
UniGeneiBt.64566.

Genome annotation databases

EnsembliENSBTAT00000015449; ENSBTAP00000015449; ENSBTAG00000011632.
GeneIDi408001.
KEGGibta:408001.

Similar proteinsi

Entry informationi

Entry nameiMLP3B_BOVIN
AccessioniPrimary (citable) accession number: O41515
Secondary accession number(s): Q6PZ01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 133 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

PubMed:9557703 sequence originates from strain Jacp of pestivirus type 1 which seems to contain a cellular insertion of part of the bovine host MAP1LC3 gene.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families