O41353 (RDRP_THOGV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase catalytic subunit
EC=2.7.7.48

Alternative name(s):
RNA-directed RNA polymerase subunit P2

Gene names

Name:

P2

Organism

Thogoto virus (isolate SiAr 126) (Tho) [Reference proteome]

Taxonomic identifier

126796 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Thogotovirus › Thogoto virus

Virus host

Amblyomma variegatum (Tropical bont tick) [TaxID: 34610]
Boophilus microplus (Cattle tick) [TaxID: 6941]
Cavia cutleri (Guinea pig) [TaxID: 10144]
Mungos mungo (Banded mongoose) [TaxID: 210652]
Rhipicephalus appendiculatus (Brown ear tick) [TaxID: 34631]

Protein attributes

Sequence length	710 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Initiates transcription by a unique cap-stealing mechanism that involves the cleavage of only the m7GpppAm structure from cellular hnRNAs.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	RNA polymerase is composed of three subunits: PA, PB1 and PB2.
Sequence similarities	Belongs to the influenza viruses polymerase PB1 family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 710	710	RNA-directed RNA polymerase catalytic subunit		PRO_0000078778
Regions
	Domain	285 – 460	176	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	O41353 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: F00BA397272E2F17	FASTA	710	81,285
	10 20 30 40 50 60 MNLFTPRSEI NPTTTQELLY AYTGPAPVAY GTRTRAVLEN IIRPYQYFYK EPNVQRALDI 70 80 90 100 110 120 KTGCKEPEDI NVEGPSSGFH TASVLKLADN FFRKYRPAME KLKYWILVKL PKLKYAELSK 130 140 150 160 170 180 GRQTYSFIHK RNLPAPIALE ETVEFLEQNL RRKIGPTLLS YCQAIADVME LDETTYEGAR 190 200 210 220 230 240 DPRPWDIQLE EIDSDEEDPL FRQVGREETY TIKFSREELW DQMRTLNTMC KHLERGRLNR 250 260 270 280 290 300 RTIATPSMLI RGFVKIVEDA AKEILENVPT SGVPVGGEEK LAKLASKQTF HTAVTGELSG 310 320 330 340 350 360 DQEKFNECLD PDAMRLMWTV FLRKLGCPDW IMELFNIPFM VFKSKLADMG EGLVYTKGKL 370 380 390 400 410 420 TDRKPLGEMP SEFDDLVRNV VGNSISCRLG MFMGMYNLTS TLLALISIER EELTGSHVES 430 440 450 460 470 480 SDDFIHFFNC KTHEEMFKQA ETLRLTLKLV GINMSPSKCI LISPAGIGEF NSKFHHRDFV 490 500 510 520 530 540 GNVATELPAL VPNGTNPMTD LAMGLNVIKH SVNTGQMNLC TGALAMRIFN HAYKYAYMAL 550 560 570 580 590 600 GVTRRTRFME ENAITPLLTN QGASPVHSFS TMHLDEVALR RHLGLLDEET LRRILNPNNP 610 620 630 640 650 660 VTQKGDPSMF FRIENKMPQI MEDYSVPSCF KYTLSRNRTI QDKPHKALLN KEERYQRVTS 670 680 690 700 710 IINKLFPEVL IQEASAPGTV RESLKRRLEL VVERSDLDEE RKKRILSRIF

References

[1]

"The fourth genus in the Orthomyxoviridae: sequence analyses of two Thogoto virus polymerase proteins and comparison with influenza viruses." Leahy M.B., Dessens J.T., Weber F., Kochs G., Nuttall P.A. Virus Res. 50:215-224(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF004985 mRNA. Translation: AAC25959.1.
RefSeq	YP_145794.1. NC_006495.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5075733.
Family and domain databases
InterPro	IPR007099. RNA-dir_pol_NSvirus. IPR001407. RNA_pol_PB1_influenza. [Graphical view]
Pfam	PF00602. Flu_PB1. 1 hit. [Graphical view]
PIRSF	PIRSF000827. RdRPol_OMV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_THOGV

Accession

Primary (citable) accession number: O41353

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program