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Protein

Probable flavin-dependent thymidylate synthase

Gene

A674R

Organism
Paramecium bursaria Chlorella virus 1 (PBCV-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP+.By similarity

Cofactori

FADBy similarityNote: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.By similarity

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei55FAD; shared with neighboring subunitsBy similarity1
Binding sitei86FAD; shared with neighboring subunitsBy similarity1
Binding sitei155dUMPBy similarity1
Binding sitei177FADBy similarity1
Active sitei182Involved in ionization of N3 of dUMP, leading to its activationBy similarity1
Binding sitei182dUMP; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 78dUMP; shared with dimeric partnerBy similarity4
Nucleotide bindingi78 – 80FADBy similarity3
Nucleotide bindingi86 – 90dUMPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable flavin-dependent thymidylate synthaseBy similarity (EC:2.1.1.148By similarity)
Short name:
FDTSBy similarity
Alternative name(s):
FAD-dependent thymidylate synthaseBy similarity
Gene namesi
Ordered Locus Names:A674R
OrganismiParamecium bursaria Chlorella virus 1 (PBCV-1)
Taxonomic identifieri10506 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostiChlorella [TaxID: 114049]
Proteomesi
  • UP000000862 Componenti: Genome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075203.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001756031 – 216Probable flavin-dependent thymidylate synthaseAdd BLAST216

Proteomic databases

PRIDEiO41156.

Interactioni

Subunit structurei

Homotetramer.By similarity

Chemistry databases

BindingDBiO41156.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 11Combined sources10
Helixi19 – 28Combined sources10
Helixi35 – 38Combined sources4
Helixi41 – 50Combined sources10
Helixi54 – 58Combined sources5
Beta strandi61 – 69Combined sources9
Helixi70 – 76Combined sources7
Beta strandi82 – 86Combined sources5
Turni89 – 91Combined sources3
Helixi126 – 146Combined sources21
Helixi151 – 154Combined sources4
Helixi155 – 157Combined sources3
Beta strandi162 – 171Combined sources10
Helixi172 – 182Combined sources11
Helixi189 – 205Combined sources17
Helixi207 – 212Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CFAX-ray2.30A/B1-216[»]
4FZBX-ray2.59A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P2-216[»]
ProteinModelPortaliO41156.
SMRiO41156.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO41156.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 216ThyXPROSITE-ProRule annotationAdd BLAST216

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi78 – 88ThyX motifPROSITE-ProRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the thymidylate synthase ThyX family.Curated
Contains 1 thyX (flavin-dependent thymidylate synthase) domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK03465.

Family and domain databases

InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O41156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAKLISVTK PVVEGVNTAE ELIAYAARVS NPENQINNKT ASGLLKYCIR
60 70 80 90 100
HKHWSIFETA FMTLELKTSR GIAAQVLRHR SFHFQEFSQR YASVMETPPP
110 120 130 140 150
HQARFQDHKN RQNSLDTVPE DDQTWWATEQ EKLYAQSMEL YNKALEKGIA
160 170 180 190 200
KECARFILPL STPTTIYMSG TIRDWIHYIE LRTSNGTQRE HIDLANACKE
210
IFIKEFPSIA KALDWV
Length:216
Mass (Da):24,902
Last modified:January 1, 1998 - v1
Checksum:i633931516599A46C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF411744 Genomic DNA. Translation: AAC96983.1.
PIRiT18176.
RefSeqiNP_049030.1. NC_000852.5.

Genome annotation databases

GeneIDi918188.
KEGGivg:918188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF411744 Genomic DNA. Translation: AAC96983.1.
PIRiT18176.
RefSeqiNP_049030.1. NC_000852.5.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CFAX-ray2.30A/B1-216[»]
4FZBX-ray2.59A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P2-216[»]
ProteinModelPortaliO41156.
SMRiO41156.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiO41156.
ChEMBLiCHEMBL1075203.

Proteomic databases

PRIDEiO41156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi918188.
KEGGivg:918188.

Phylogenomic databases

KOiK03465.

Enzyme and pathway databases

UniPathwayiUPA00575.

Miscellaneous databases

EvolutionaryTraceiO41156.
PROiO41156.

Family and domain databases

InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHYX_PBCV1
AccessioniPrimary (citable) accession number: O41156
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.