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O41156

- THYX_PBCV1

UniProt

O41156 - THYX_PBCV1

Protein

Probable thymidylate synthase

Gene

A674R

Organism
Paramecium bursaria Chlorella virus 1 (PBCV-1)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate.PROSITE-ProRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP+.PROSITE-ProRule annotation

    Cofactori

    Binds 1 FAD per subunit.PROSITE-ProRule annotation

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. thymidylate synthase (FAD) activity Source: UniProtKB-EC

    GO - Biological processi

    1. dTMP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable thymidylate synthase (EC:2.1.1.148)
    Short name:
    TS
    Short name:
    TSase
    Gene namesi
    Ordered Locus Names:A674R
    OrganismiParamecium bursaria Chlorella virus 1 (PBCV-1)
    Taxonomic identifieri10506 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
    Virus hostiChlorella [TaxID: 114049]
    ProteomesiUP000000862: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 216216Probable thymidylate synthasePRO_0000175603Add
    BLAST

    Structurei

    Secondary structure

    1
    216
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 1110
    Helixi19 – 2810
    Helixi35 – 384
    Helixi41 – 5010
    Helixi54 – 585
    Beta strandi61 – 699
    Helixi70 – 767
    Beta strandi82 – 865
    Turni89 – 913
    Helixi126 – 14621
    Helixi151 – 1544
    Helixi155 – 1573
    Beta strandi162 – 17110
    Helixi172 – 18211
    Helixi189 – 20517
    Helixi207 – 2126

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CFAX-ray2.30A/B1-216[»]
    4FZBX-ray2.59A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P2-216[»]
    ProteinModelPortaliO41156.
    SMRiO41156. Positions 1-216.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO41156.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 216216ThyXPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the thymidylate synthase ThyX family.Curated
    Contains 1 thyX (flavin-dependent thymidylate synthase) domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR003669. Thymidylate_synthase_ThyX.
    [Graphical view]
    PfamiPF02511. Thy1. 1 hit.
    [Graphical view]
    SUPFAMiSSF69796. SSF69796. 1 hit.
    TIGRFAMsiTIGR02170. thyX. 1 hit.
    PROSITEiPS51331. THYX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O41156-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAKLISVTK PVVEGVNTAE ELIAYAARVS NPENQINNKT ASGLLKYCIR    50
    HKHWSIFETA FMTLELKTSR GIAAQVLRHR SFHFQEFSQR YASVMETPPP 100
    HQARFQDHKN RQNSLDTVPE DDQTWWATEQ EKLYAQSMEL YNKALEKGIA 150
    KECARFILPL STPTTIYMSG TIRDWIHYIE LRTSNGTQRE HIDLANACKE 200
    IFIKEFPSIA KALDWV 216
    Length:216
    Mass (Da):24,902
    Last modified:January 1, 1998 - v1
    Checksum:i633931516599A46C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF411744 Genomic DNA. Translation: AAC96983.1.
    PIRiT18176.
    RefSeqiNP_049030.1. NC_000852.5.

    Genome annotation databases

    GeneIDi918188.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF411744 Genomic DNA. Translation: AAC96983.1 .
    PIRi T18176.
    RefSeqi NP_049030.1. NC_000852.5.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CFA X-ray 2.30 A/B 1-216 [» ]
    4FZB X-ray 2.59 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P 2-216 [» ]
    ProteinModelPortali O41156.
    SMRi O41156. Positions 1-216.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1075203.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 918188.

    Miscellaneous databases

    EvolutionaryTracei O41156.

    Family and domain databases

    InterProi IPR003669. Thymidylate_synthase_ThyX.
    [Graphical view ]
    Pfami PF02511. Thy1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69796. SSF69796. 1 hit.
    TIGRFAMsi TIGR02170. thyX. 1 hit.
    PROSITEi PS51331. THYX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of 74 kb of DNA located at the right end of the 330-kb chlorella virus PBCV-1 genome."
      Li Y., Lu Z., Sun L., Ropp S., Kutish G.F., Rock D.L., van Etten J.L.
      Virology 237:360-377(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiTHYX_PBCV1
    AccessioniPrimary (citable) accession number: O41156
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3