ID   O41063_PBCV1            Unreviewed;       265 AA.
AC   O41063;
DT   01-JAN-1998, integrated into UniProtKB/TrEMBL.
DT   01-JAN-1998, sequence version 1.
DT   13-SEP-2023, entry version 88.
DE   RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE            EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN   Name=A581R {ECO:0000313|EMBL:AAC96931.1};
OS   Paramecium bursaria Chlorella virus 1 (PBCV-1).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC   Algavirales; Phycodnaviridae; Chlorovirus.
OX   NCBI_TaxID=10506 {ECO:0000313|EMBL:AAC96931.1, ECO:0000313|Proteomes:UP000000862};
OH   NCBI_TaxID=3071; Chlorella.
RN   [1] {ECO:0000313|EMBL:AAC96931.1, ECO:0000313|Proteomes:UP000000862}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=7831789; DOI=10.1016/S0042-6822(95)80049-2;
RA   Lu Z., Li Y., Zhang Y., Kutish G.F., Rock D.L., Van Etten J.L.;
RT   "Analysis of 45 kb of DNA located at the left end of the chlorella virus
RT   PBCV-1 genome.";
RL   Virology 206:339-352(1995).
RN   [2] {ECO:0000313|EMBL:AAC96931.1, ECO:0000313|Proteomes:UP000000862}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=7676624; DOI=10.1006/viro.1995.1462;
RA   Li Y., Lu Z., Burbank D.E., Kutish G.F., Rock D.L., Van Etten J.L.;
RT   "Analysis of 43 kb of the Chlorella virus PBCV-1 330-kb genome: map
RT   positions 45 to 88.";
RL   Virology 212:134-150(1995).
RN   [3] {ECO:0000313|EMBL:AAC96931.1, ECO:0000313|Proteomes:UP000000862}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8614977; DOI=10.1006/viro.1996.0038;
RA   Lu Z., Li Y., Que Q., Kutish G.F., Rock D.L., Van Etten J.L.;
RT   "Analysis of 94 kb of the chlorella virus PBCV-1 330-kb genome: map
RT   positions 88 to 182.";
RL   Virology 216:102-123(1996).
RN   [4] {ECO:0000313|EMBL:AAC96931.1, ECO:0000313|Proteomes:UP000000862}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8806566; DOI=10.1006/viro.1996.0482;
RA   Kutish G.F., Li Y., Lu Z., Furuta M., Rock D.L., Van Etten J.L.;
RT   "Analysis of 76 kb of the chlorella virus PBCV-1 330-kb genome: map
RT   positions 182 to 258.";
RL   Virology 223:303-317(1996).
RN   [5] {ECO:0000313|EMBL:AAC96931.1, ECO:0000313|Proteomes:UP000000862}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9356347; DOI=10.1006/viro.1997.8805;
RA   Li Y., Lu Z., Sun L., Ropp S., Kutish G.F., Rock D.L., Van Etten J.L.;
RT   "Analysis of 74 kb of DNA located at the right end of the 330-kb chlorella
RT   virus PBCV-1 genome.";
RL   Virology 237:360-377(1997).
RN   [6] {ECO:0000313|EMBL:AAC96931.1, ECO:0000313|Proteomes:UP000000862}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10544099; DOI=10.1006/viro.1999.9972;
RA   Kaiser A., Vollmert M., Tholl D., Graves M.V., Gurnon J.R., Xing W.,
RA   Lisec A.D., Nickerson K.W., Van Etten J.L.;
RT   "Chlorella virus PBCV-1 encodes a functional homospermidine synthase.";
RL   Virology 263:254-262(1999).
RN   [7] {ECO:0000313|EMBL:AAC96931.1, ECO:0000313|Proteomes:UP000000862}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11021991; DOI=10.1006/viro.2000.0500;
RA   Sun L., Gurnon J.R., Adams B.J., Graves M.V., Van Etten J.L.;
RT   "Characterization of a beta-1,3-glucanase encoded by chlorella virus PBCV-
RT   1.";
RL   Virology 276:27-36(2000).
RN   [8] {ECO:0000313|EMBL:AAC96931.1, ECO:0000313|Proteomes:UP000000862}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=19828609; DOI=10.1128/JVI.01698-09;
RA   Yanai-Balser G.M., Duncan G.A., Eudy J.D., Wang D., Li X., Agarkova I.V.,
RA   Dunigan D.D., Van Etten J.L.;
RT   "Microarray analysis of Paramecium bursaria chlorella virus 1
RT   transcription.";
RL   J. Virol. 84:532-542(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC         N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC         COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC         Evidence={ECO:0000256|ARBA:ARBA00001279};
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC       {ECO:0000256|ARBA:ARBA00006594}.
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DR   EMBL; JF411744; AAC96931.1; -; Genomic_DNA.
DR   PIR; T18083; T18083.
DR   RefSeq; NP_048937.1; NC_000852.5.
DR   REBASE; 3351; M.CviAI.
DR   GeneID; 918011; -.
DR   KEGG; vg:918011; -.
DR   OrthoDB; 8399at10239; -.
DR   Proteomes; UP000000862; Genome.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.10.1020.10; Adenine-specific Methyltransferase, Domain 2; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR023095; Ade_MeTrfase_dom_2.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR012263; M_m6A_EcoRV.
DR   InterPro; IPR012327; MeTrfase_D12.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   NCBIfam; TIGR00571; dam; 1.
DR   PANTHER; PTHR30481; DNA ADENINE METHYLASE; 1.
DR   PANTHER; PTHR30481:SF3; DNA ADENINE METHYLASE; 1.
DR   Pfam; PF02086; MethyltransfD12; 1.
DR   PIRSF; PIRSF000398; M_m6A_EcoRV; 1.
DR   PRINTS; PR00505; D12N6MTFRASE.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW   ECO:0000313|EMBL:AAC96931.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000862};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   COILED          64..94
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   265 AA;  30522 MW;  228234EBA10E397E CRC64;
     MSSPFLKWVG GKQKFVNNII THFPKNIDTY HEPFLGGGSV LLAVLNSDIQ IKKIRANDLN
     AYLIQTYIDV KDNTEELIEE LKSLKNSEED YYENRKIFNS LKKEGHFSVK ASALFIYLNK
     TCFRGLYREG PNGFNVPFGH YKNPNWINAD LLRGWSDKIK NVEFSCLPYQ DFLNDVGEHD
     FVYLDPPYAP ENKTSFTKYN VSDFLDHEQL FSIVKSLPQF VMSNSKTEKT LNAFASFEIL
     EITTRRAINS KKPGSTTTEI IVIKM
//