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Protein

Replication-associated protein A

Gene

C1

Organism
Maize streak virus genotype C (isolate Set) (MSV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Implicated in enhancement of V-sense gene expression. Acts a an inhibitor of C-sense gene transcription (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52Divalent metal cationSequence analysis1
Metal bindingi62Divalent metal cationSequence analysis1
Active sitei100For DNA cleavage activityBy similarity1
Metal bindingi104Divalent metal cationSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Endonuclease, Hydrolase, Nuclease, Repressor

Keywords - Biological processi

G1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Modulation of host cell cycle by virus

Keywords - Ligandi

DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein A (EC:3.1.21.-)
Short name:
RepA
Gene namesi
ORF Names:C1
OrganismiMaize streak virus genotype C (isolate Set) (MSV)
Taxonomic identifieri268344 [NCBI]
Taxonomic lineageiVirusesssDNA virusesGeminiviridaeMastrevirus
Virus hostiAvena sativa (Oat) [TaxID: 4498]
Axonopus compressus [TaxID: 217170]
Brachiaria deflexa [TaxID: 240436]
Cenchrus polystachios [TaxID: 281129]
Coix lacryma-jobi (Job's tears) [TaxID: 4505]
Dactyloctenium aegyptium [TaxID: 270102]
Digitaria [TaxID: 66017]
Echinochloa colona [TaxID: 90396]
Eleusine coracana (Indian finger millet) (Ragi) [TaxID: 4511]
Eleusine indica (Goosegrass) (Cynosurus indicus) [TaxID: 29674]
Hordeum vulgare (Barley) [TaxID: 4513]
Megathyrsus maximus [TaxID: 59788]
Melinis repens (Natal redtop) (Rhynchelytrum repens) [TaxID: 29709]
Oryza glaberrima (African rice) [TaxID: 4538]
Oryza sativa (Rice) [TaxID: 4530]
Paspalum conjugatum (Hilo grass) [TaxID: 158143]
Paspalum notatum (Bahia grass) [TaxID: 147272]
Paspalum scrobiculatum [TaxID: 173849]
Pennisetum americanum (Pearl millet) (Pennisetum glaucum) [TaxID: 4543]
Rottboellia cochinchinensis [TaxID: 300125]
Saccharum officinarum (Sugarcane) [TaxID: 4547]
Setaria barbata [TaxID: 192628]
Triticum aestivum (Wheat) [TaxID: 4565]
Zea mays (Maize) [TaxID: 4577]
Proteomesi
  • UP000008872 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003169351 – 272Replication-associated protein AAdd BLAST272

Interactioni

Subunit structurei

Homooligomer. Interacts (via LXCXE domain) with host retinoblastoma-related protein 1 (RBR1), and may thereby deregulate the host cell cycle. Part of the C- and V-complexes which are RepA-Rep-DNA complexes involved in the c-sense and v-sense transcription (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO40987.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni175 – 187OligomerizationBy similarityAdd BLAST13
Regioni221 – 230TransactivationBy similarity10

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi18 – 22RCR-15
Motifi60 – 65RCR-26
Motifi100 – 103RCR-34
Motifi198 – 202LXCXE motif, interaction with host RBR1By similarity5

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 may be involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.
The LXCXE motif specifically binds to host RBR1.By similarity

Sequence similaritiesi

Belongs to the geminiviridae Rep protein family.Curated

Family and domain databases

InterProiIPR001146. Gemini_AL1_MSV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
[Graphical view]
PfamiPF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
[Graphical view]
PRINTSiPR00227. GEMCOATAL1.
PR00229. GEMCOATMSVL1.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform RepA (identifier: O40987-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSSSSNRPF SHRSPNTFLT YPQCPEQPEI ISQRIWDLCS HWTPLYIICA
60 70 80 90 100
REAHRDGNQC LHALIQTEKP VRTTDSRFFD IDGFHPNIQS AISPNKVRDY
110 120 130 140 150
ITKEPLALFE RGTFIPRKKS FLGNSSKGNS DKKPSKDEIM RDIISHATSK
160 170 180 190 200
QEYLSMVQKS LPYDWSTKLQ YFEYSANKLF PDIQEEFINP HPTSEPDLLC
210 220 230 240 250
NESIKDWLQP NIYQVSPQAY LLLQPNCYSI DDAISDLEWL DDLTSKQIME
260 270
QGSAASTSSV QQGQENLHGP EA
Note: Produced from the unspliced transcript.
Length:272
Mass (Da):31,178
Last modified:January 1, 1998 - v1
Checksum:iD6ABBD1178D472E6
GO
Isoform Rep (identifier: O40986-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry O40986.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:354
Mass (Da):41,330
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007881 Genomic DNA. Translation: AAB63456.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007881 Genomic DNA. Translation: AAB63456.1.

3D structure databases

ProteinModelPortaliO40987.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001146. Gemini_AL1_MSV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
[Graphical view]
PfamiPF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
[Graphical view]
PRINTSiPR00227. GEMCOATAL1.
PR00229. GEMCOATMSVL1.
ProtoNetiSearch...

Entry informationi

Entry nameiREPA_MSVSE
AccessioniPrimary (citable) accession number: O40987
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.