ID   ORF2A_CMVNT             Reviewed;         857 AA.
AC   O40977;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 40.
DE   RecName: Full=RNA-directed RNA polymerase 2A;
DE            Short=protein 2A;
DE            EC=2.7.7.48;
GN   Name=RNA2; ORFNames=ORF2a;
OS   Cucumber mosaic virus (strain NT9) (CMV).
OC   Viruses; ssRNA positive-strand viruses, no DNA stage; Bromoviridae;
OC   Cucumovirus.
OX   NCBI_TaxID=117124;
OH   NCBI_TaxID=3562; Spinacia oleracea (Spinach).
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   MEDLINE=96105341; PubMed=7503683; DOI=10.1007/BF01384346;
RA   Hsu Y.-H., Wu C.W., Lin B.Y., Chen H.Y., Lee M.F., Tsai C.H.;
RT   "Complete genomic RNA sequences of cucumber mosaic virus strain NT9
RT   from Taiwan.";
RL   Arch. Virol. 140:1841-1847(1995).
CC   -!- FUNCTION: RNA-directed RNA polymerase.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- MISCELLANEOUS: The virus has a tripartite genome (RNA1, RNA2 and
CC       RNA3) and an encapsidated subgenomic RNA (RNA4) from which the
CC       coat protein is expressed. Virions are icosahedral, not enveloped,
CC       with 32 capsomers per nucleocapsid.
CC   -!- SIMILARITY: Belongs to the cucumoviruses/bromoviruses 2A family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; D28779; BAA21694.1; -; Genomic_RNA.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR001788; RNA-dep_RNA_pol_vir-typ.
DR   InterPro; IPR007094; RNA_pol_PSvir.
DR   Pfam; PF00978; RdRP_2; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA replication;
KW   RNA-directed RNA polymerase; Transferase.
FT   CHAIN         1    857       RNA-directed RNA polymerase 2A.
FT                                /FTId=PRO_0000083279.
FT   DOMAIN      511    624       RdRp catalytic.
SQ   SEQUENCE   857 AA;  96561 MW;  E5CCF99D4586AEAE CRC64;
     MAFSAPTFSL ANLLNGSYGV DTPEEVERVR SEQREEAAAA CRNYRPLPAV DVSESVTEDA
     HSLRTPDGAP SEEVSVEFVT YGAEDYLEKS DDELSVAFET MVKPMRIGQL WCPAFNKCSF
     ISSIAMARAL LLAPRTSHRT MKGFEDLVAA IYTKSDFYYD EECEADDIQI DVSSRDVPGY
     SFEPWSRTSG FEPPPICEAC DMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVDNA
     TLLSNLGPFL VPVKCQYEKC PTPTVAIPPS LNRATDRVDI NLVQSICDST LPTHSNYDDS
     FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLS TGSGHKRVGT TKEVLTAIKK
     RNADVPELGD SVNLSRLSKA VAERFFISYI NGNSLASSNF VNVVSNFHDY MEKWKSSGLS
     YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKGITS QFSPLFTALF
     ERFQRCLRER IILPVGKISS LEMAGFDVKN KHCLEIDLSK FDKSQGEFHL MIQEHILNGL
     GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAF TYFGNTIVTM AEFAWCYDTD
     QFEKLLFSGD DSLGFSVLPP VGDPSKFTTL FNMEAKVMEP AVPYICSKFL LSDEFGNTFS
     VPDPLREVQR LGTKKIPYSD NDEFLFAHFM SFVDRLKFLD RMTQSCIDQL SLFFELKYRK
     SGAEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFS CVELRIERSS STKQRKKKDG
     IERRRSDKRR TPTGSYGGGE KTETKVSHEE STGTRSQKSQ REGAFKSQIV PLPTILSGGW
     SGTDRAPPCE HGGIIRI
//