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Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (strain RVA/Human/Japan/K8/1977/G1P3A[9]) (RV-A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri42 – 79Sequence analysisAdd BLAST38

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1
Short name:
NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name:
NS53
OrganismiRotavirus A (strain RVA/Human/Japan/K8/1977/G1P3A[9]) (RV-A)
Taxonomic identifieri39012 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003690831 – 486Non-structural protein 1Add BLAST486

Interactioni

Subunit structurei

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 81RNA-bindingBy similarityAdd BLAST81
Regioni82 – 176Important for cytoskeleton localizationBy similarityAdd BLAST95
Regioni313 – 486Interaction with IRF3By similarityAdd BLAST174

Domaini

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity (By similarity).By similarity

Sequence similaritiesi

Belongs to the rotavirus A NSP1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri42 – 79Sequence analysisAdd BLAST38

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O40627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATFKDACYH YKRINKLNNN SLKLGVNDTW RPSPPTKYKG WCLDCCQHTD
60 70 80 90 100
LTYCSGCTMY HVCQWCSQYG RCFLDNEPHL LRMRTFKNEV TKDDLKNLID
110 120 130 140 150
MYETLFPMNH KIVCRFINTA RQHKCRNECM TQWYNHLLMP ITLQSLSIEL
160 170 180 190 200
DGDVYYVFGY YDNMNNINQT PFSFINLVDI YDKLLLDDVN FTRMSFLPAS
210 220 230 240 250
LQQEYALRYF SKSRFINEQR KCVNDSHFSI NVLENLHNPS FKIQITRNCS
260 270 280 290 300
ELSFDWNEAC KLVKNVSAYF DMLKTSHIEF YSVSTRCRIF TQCKLKMASK
310 320 330 340 350
LIKPNYITSN HKTLATEVHN CKWCSVNNSY TVWNDFRIKK IYDNIFNFLR
360 370 380 390 400
ALVKSNVNIG HCSSQEKIYE YVKDVLNVCD DERWKTSIME IFNCLEPVEL
410 420 430 440 450
DDVKYVLFNH EINWDVINVL VHSIGKVPQI LTLENVITIM QSIIYEWFDI
460 470 480
RYMRNTPMVT FTIDKLRRLY TGLKTVEYDS GISDIE
Length:486
Mass (Da):57,680
Last modified:January 1, 1998 - v1
Checksum:i0C96042E585EA63A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38152 Genomic RNA. Translation: BAA20543.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38152 Genomic RNA. Translation: BAA20543.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSP1_ROTHJ
AccessioniPrimary (citable) accession number: O40627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.