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Protein

Replication-associated protein A

Gene

C1

Organism
Bean yellow dwarf virus (BeYDV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in enhancement of V-sense gene expression. Acts a an inhibitor of C-sense gene transcription.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Divalent metal cationSequence analysis1
Metal bindingi58Divalent metal cationSequence analysis1
Metal bindingi60Divalent metal cationSequence analysis1
Active sitei98For DNA cleavage activityBy similarity1
Metal bindingi102Divalent metal cationSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Endonuclease, Hydrolase, Nuclease, Repressor

Keywords - Biological processi

G1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Modulation of host cell cycle by virus

Keywords - Ligandi

DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein A (EC:3.1.21.-)
Short name:
RepA
Gene namesi
ORF Names:C1
OrganismiBean yellow dwarf virus (BeYDV)
Taxonomic identifieri57119 [NCBI]
Taxonomic lineageiVirusesssDNA virusesGeminiviridaeMastrevirus
Virus hostiPhaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Proteomesi
  • UP000007453 Componenti: Genome

Subcellular locationi

  • Host nucleus 1 Publication
  • Host cytoplasm 1 Publication

  • Note: distributed equally throughout both the nucleus and the cytoplasm.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi181L → I: 20% loss of ability to bind RBR. 1 Publication1
Mutagenesisi183C → S: 50% loss of ability to bind RBR. 1 Publication1
Mutagenesisi185E → Q: 95% loss of ability to bind RBR. Reduced replication efficiency. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003187701 – 292Replication-associated protein AAdd BLAST292

Interactioni

Subunit structurei

Homooligomer. Interacts with host retinoblastoma-related protein 1 (RBR1), and may thereby deregulate the host cell cycle. Part of the C- and V-complexes which are RepA-Rep-DNA complexes involved in the c-sense and v-sense transcription (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO39521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni160 – 172OligomerizationBy similarityAdd BLAST13
Regioni181 – 185Binding to RBR15

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi16 – 20RCR-15
Motifi58 – 63RCR-26
Motifi98 – 101RCR-34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi282 – 287Poly-Ser6

Domaini

There are three rolling circle replication (RCR) motifs. RCR-2 may be involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similaritiesi

Belongs to the geminiviridae Rep protein family.Curated

Family and domain databases

InterProiIPR001146. Gemini_AL1_MSV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
[Graphical view]
PfamiPF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
[Graphical view]
PRINTSiPR00227. GEMCOATAL1.
PR00229. GEMCOATMSVL1.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform RepA (identifier: O39521-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPSASKNFRL QSKYVFLTYP KCSSQRDDLF QFLWEKLTPF LIFFLGVASE
60 70 80 90 100
LHQDGTTHYH ALIQLDKKPC IRDPSFFDFE GNHPNIQPAR NSKQVLDYIS
110 120 130 140 150
KDGDIKTRGD FRDHKVSPRK SDARWRTIIQ TATSKEEYLD MIKEEFPHEW
160 170 180 190 200
ATKLQWLEYS ANKLFPPQPE QYVSPFTESD LRCHEDLHNW RETHLYHVSI
210 220 230 240 250
DAYTFIHPVS YDQAQSDLEW MADLTRMREG LGSDTPASTS ADQLVPERPP
260 270 280 290
GLEVSGDTTT GTGPSTSPTT MNTPPIISST TSPSSSSHCG SN
Note: Produced from the unspliced transcript.
Length:292
Mass (Da):33,354
Last modified:January 1, 1998 - v1
Checksum:i31C4DCA03607F3BF
GO
Isoform Rep (identifier: O39522-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry O39522.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:334
Mass (Da):39,291
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti63I → L in strain: Mild. 1
Natural varianti171Q → P in strain: Mild. 1
Natural varianti189N → S in strain: Mild. 1
Natural varianti205F → Y in strain: Mild. 1
Natural varianti212D → Q in strain: Mild. 1
Natural varianti227 – 228MR → TM in strain: Mild. 2
Natural varianti231 – 232LG → ME in strain: Mild. 2
Natural varianti256G → D in strain: Mild. 1
Natural varianti260T → I in strain: Mild. 1
Natural varianti263G → V in strain: Mild. 1
Natural varianti266T → I in strain: Mild. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11023 Genomic DNA. Translation: CAA71907.1.
DQ458791 Genomic DNA. Translation: ABE67103.1.
RefSeqiNP_612222.1. NC_003493.2. [O39521-1]

Genome annotation databases

GeneIDi935291.
KEGGivg:935291.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11023 Genomic DNA. Translation: CAA71907.1.
DQ458791 Genomic DNA. Translation: ABE67103.1.
RefSeqiNP_612222.1. NC_003493.2. [O39521-1]

3D structure databases

ProteinModelPortaliO39521.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi935291.
KEGGivg:935291.

Family and domain databases

InterProiIPR001146. Gemini_AL1_MSV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
[Graphical view]
PfamiPF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
[Graphical view]
PRINTSiPR00227. GEMCOATAL1.
PR00229. GEMCOATMSVL1.
ProtoNetiSearch...

Entry informationi

Entry nameiREPA_BEYDV
AccessioniPrimary (citable) accession number: O39521
Secondary accession number(s): A4K7Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.