ID   ORF2A_CMVAS             Reviewed;         858 AA.
AC   O39436;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 38.
DE   RecName: Full=RNA-directed RNA polymerase 2A;
DE            Short=protein 2A;
DE            EC=2.7.7.48;
GN   Name=RNA2; ORFNames=ORF2a;
OS   Cucumber mosaic virus (strain As) (CMV).
OC   Viruses; ssRNA positive-strand viruses, no DNA stage; Bromoviridae;
OC   Cucumovirus.
OX   NCBI_TaxID=117118;
OH   NCBI_TaxID=3562; Spinacia oleracea (Spinach).
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Kim S.H., Park Y.I.;
RL   Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-directed RNA polymerase.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- MISCELLANEOUS: The virus has a tripartite genome (RNA1, RNA2 and
CC       RNA3) and an encapsidated subgenomic RNA (RNA4) from which the
CC       coat protein is expressed. Virions are icosahedral, not enveloped,
CC       with 32 capsomers per nucleocapsid.
CC   -!- SIMILARITY: Belongs to the cucumoviruses/bromoviruses 2A family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; AF033667; AAB86650.1; -; Genomic_RNA.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR001788; RNA-dep_RNA_pol_vir-typ.
DR   InterPro; IPR007094; RNA_pol_PSvir.
DR   Pfam; PF00978; RdRP_2; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA replication;
KW   RNA-directed RNA polymerase; Transferase.
FT   CHAIN         1    858       RNA-directed RNA polymerase 2A.
FT                                /FTId=PRO_0000083271.
FT   DOMAIN      511    624       RdRp catalytic.
SQ   SEQUENCE   858 AA;  96809 MW;  5AF06495CBABA473 CRC64;
     MAFPAPAFSL ANLLNGSYGV DTPEEVERVR SEQRDEAAAA CRNYRPLPAV DVSESVPEDA
     HSLRTPDGAP SEEVSVEFVT YGAEDYLEKS DDELLVAFET MVKPMRIGQL WCPAFNKCSF
     ISSIAMARAL LLAPRTSHRT MKCFEDLVAA IYTKSDFYYD DECEADDVQI DISSRDVPGY
     SFEPWSRTSG FEPPPICEAC DMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVNNA
     TLSSNLGPFL VPVKCPYEKC PTPVGEIPPD LNRATDRVDI NLVQSICDST LPTHSNYDDS
     FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK
     RNADVPELGD SVNLSRLSKA VAERFFISYI NGNSLASSNF VNVVSNFHDY MEKWKSSGLS
     YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKGITS QFSPLFTALF
     ERFQRCLRER IILPVGKISS LEMAGFDVKN KHCFEIDLSK FDKSQGEFPF KIQEHILNGL
     GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAF TYFGNTIVTM AEFAWCYDTD
     QFEKLLFSGD DSLGFSVLPP VGDPSKFTTL FNMEAKVMEP AVPYICSKFL LSDEFGNTFS
     VPDPLREVQR LGTKKIPCSD NDEFLFAHFM SFVDRLKFLD RMTQSCIDQL SLFFELKYRK
     SGEEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFS CVELRIERSS STKQREKKDG
     IERRRNDKRR TPTGSYGGGE EAETKVSQAE STGTRSQKSQ REGAFKSQAV PLPTILSSRW
     FGTDRDVPPC EHGGIVRV
//