O39436 (RDRP_CMVAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase 2a
Short name=protein 2a
EC=2.7.7.48

Gene names

ORF Names:

ORF2a

Organism

Cucumber mosaic virus (strain As) (CMV)

Taxonomic identifier

117118 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Bromoviridae › Cucumovirus

Virus host

Spinacia oleracea (Spinach) [TaxID: 3562]
Cucumis sativus (Cucumber) [TaxID: 3659]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]

Protein attributes

Sequence length	858 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which replicates the viral genome composed of 3 RNA segments, RNA1, RNA2 and RNA3 Potential.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Interacts with replication protein 1a By similarity.
Sequence similarities	Belongs to the ssRNA positive-strand viruses RNA-directed RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 858	858	RNA-directed RNA polymerase 2a		PRO_0000083271
Regions
	Domain	511 – 624	114	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	O39436 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5AF06495CBABA473	FASTA	858	96,809
	10 20 30 40 50 60 MAFPAPAFSL ANLLNGSYGV DTPEEVERVR SEQRDEAAAA CRNYRPLPAV DVSESVPEDA 70 80 90 100 110 120 HSLRTPDGAP SEEVSVEFVT YGAEDYLEKS DDELLVAFET MVKPMRIGQL WCPAFNKCSF 130 140 150 160 170 180 ISSIAMARAL LLAPRTSHRT MKCFEDLVAA IYTKSDFYYD DECEADDVQI DISSRDVPGY 190 200 210 220 230 240 SFEPWSRTSG FEPPPICEAC DMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVNNA 250 260 270 280 290 300 TLSSNLGPFL VPVKCPYEKC PTPVGEIPPD LNRATDRVDI NLVQSICDST LPTHSNYDDS 310 320 330 340 350 360 FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK 370 380 390 400 410 420 RNADVPELGD SVNLSRLSKA VAERFFISYI NGNSLASSNF VNVVSNFHDY MEKWKSSGLS 430 440 450 460 470 480 YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKGITS QFSPLFTALF 490 500 510 520 530 540 ERFQRCLRER IILPVGKISS LEMAGFDVKN KHCFEIDLSK FDKSQGEFPF KIQEHILNGL 550 560 570 580 590 600 GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAF TYFGNTIVTM AEFAWCYDTD 610 620 630 640 650 660 QFEKLLFSGD DSLGFSVLPP VGDPSKFTTL FNMEAKVMEP AVPYICSKFL LSDEFGNTFS 670 680 690 700 710 720 VPDPLREVQR LGTKKIPCSD NDEFLFAHFM SFVDRLKFLD RMTQSCIDQL SLFFELKYRK 730 740 750 760 770 780 SGEEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFS CVELRIERSS STKQREKKDG 790 800 810 820 830 840 IERRRNDKRR TPTGSYGGGE EAETKVSQAE STGTRSQKSQ REGAFKSQAV PLPTILSSRW 850 FGTDRDVPPC EHGGIVRV

References

[1]	Kim S.H., Park Y.I. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF033667 Genomic RNA. Translation: AAB86650.1.
3D structure databases
ProteinModelPortal	O39436.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007094. RNA-dir_pol_PSvirus. IPR001788. Tymovirus_RNA-dep_RNA_pol. [Graphical view]
Pfam	PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_CMVAS

Accession

Primary (citable) accession number: O39436

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 28, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program