O37319 (NTP1_HAEPV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside triphosphatase I
EC=3.6.1.15

Alternative name(s):
NPH-I
Nucleoside triphosphate phosphohydrolase I
Short name=NPH I

Gene names

Name:	NPH1
ORF Names:	F1L

Organism

Heliothis armigera entomopoxvirus (HaEPV)

Taxonomic identifier

10290 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Entomopoxvirinae › Betaentomopoxvirus

Virus host

Lepidoptera (butterflies and moths) [TaxID: 7088]

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent ATPase required for providing the needed energy to achieve the termination of early transcripts. Acts in concert with the RAP94 subunit of the virion RNA polymerase and the capping enzyme/VTF to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex. NPH-I must bind ssDNA in order to exhibit ATPase activity By similarity.
Catalytic activity	NTP + H2O = NDP + phosphate.
Subunit structure	Monomer. Interacts (via C-terminus) with RAP94 (via N-terminus). Interacts with the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity.
Subcellular location	Virion By similarity. Note: Virion core enzyme By similarity.
Sequence similarities	Belongs to the helicase family. NPH I subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Virion
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Nucleoside triphosphatase I		PRO_0000099102
Regions
	Domain	48 – 213	166	Helicase ATP-binding
	Domain	377 – 540	164	Helicase C-terminal
	Nucleotide binding	61 – 68	8	ATP By similarity
	Region	466 – 532	67	Binding to the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity
	Motif	151 – 154	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	O37319 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: AA1B63287F8B8034	FASTA	646	75,763
	10 20 30 40 50 60 MFTLDSIVGA HINYALDKTA HLPETITNNV SNVEITLKDY QYFVSRVFIG LKNLNSMLLF 70 80 90 100 110 120 WDTGMGKTLT AVYIIKHIKE LFPRWIILIF IKKSLYIDPW LNTIRAFAPD ISNNIIFVYY 130 140 150 160 170 180 DSSSSLDKFV NIYRSIENSL NKKNRLLIII DEVHKLISRS VKKDNSERNF TPTYKKLIKL 190 200 210 220 230 240 ANYEGNKILC MSATPIYNNI DEFNNLIGLL RPNVMTFKEE YIINGKLINF KEIRDTLLGL 250 260 270 280 290 300 CSYKRLIEAD SFTETNYVEG YAKKNVIYHN IIMSEEQSKL FNLAEKYDYK TELGGLKTMR 310 320 330 340 350 360 RLVSSFAFYD LKIKGDLNNI EYNDMIKRKL AEFSEFTKTI NFSNNFIEKF KRDDLKNDIL 370 380 390 400 410 420 LEDINNYNIL YQYSCKYIEA CRIILNSRGK VLLFEPLVNF EGIASLKYYF NCFNITYVEY 430 440 450 460 470 480 SSKTLKTRDI EISEYNNYEN NDGKNIKVCI FSYAGSEGIS FKCVNDIIIL DMPWNESELK 490 500 510 520 530 540 QIMGRSIRLN SHNDLPLENR YVNVHFIISY TNNRKSVDKE ILDIIKDKQS KINVVFDLLK 550 560 570 580 590 600 SISIESIHNI HKYIEPVESE TIFDTIRKTR MREMNISNVI VKIKLYPIMY CKDYDRATIL 610 620 630 640 KGLLNKENNI IYNEDTAVAK LTIENNKPVF IIVDDNLIYI ADDYYE

References

[1]

"Mapping of the Heliothis armigera entomopoxvirus (HaEPV) genome, and analysis of genes encoding the HaEPV spheroidin and nucleoside triphosphate phosphohydrolase I proteins." Sriskantha A., Osborne R.J., Dall D.J. J. Gen. Virol. 78:3115-3123(1997) [PubMed: 9400959] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF019224 Genomic DNA. Translation: AAB96624.1.
3D structure databases
ProteinModelPortal	O37319.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR001650. Helicase_C. IPR013676. NPHI_C. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF08469. NPHI_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP1_HAEPV

Accession

Primary (citable) accession number: O37319

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program