Reviewed, UniProtKB/Swiss-Prot O37319 (NTP1_HAEPV)

Names and origin

Protein names

Recommended name:
    Nucleoside triphosphatase I
    EC=3.6.1.15
Alternative name(s):
    Nucleoside triphosphate phosphohydrolase I
      Short name=NPH I

Gene names

Name:	NPH1
ORF Names:	F1L

Organism

Heliothis armigera entomopoxvirus (HaEPV)

Taxonomic identifier

10290 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Entomopoxvirinae › Betaentomopoxvirus

Virus host

Lepidoptera (butterflies and moths) [TaxID: 7088]

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serves two roles in transcription; it acts in concert with viral termination factor/capping enzyme to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex, and it acts by itself as a polymerase elongation factor to facilitate readthrough of intrinsic pause sites By similarity.
Catalytic activity	NTP + H2O = NDP + phosphate.
Sequence similarities	Belongs to the helicase family. NPH I subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Nucleoside triphosphatase I		PRO_0000099102
Regions
	Domain	48 – 213	166	Helicase ATP-binding
	Domain	377 – 540	164	Helicase C-terminal
	Nucleotide binding	61 – 68	8	ATP By similarity
	Motif	151 – 154	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	O37319-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: AA1B63287F8B8034	FASTA	646	75,763
	10 20 30 40 50 60 MFTLDSIVGA HINYALDKTA HLPETITNNV SNVEITLKDY QYFVSRVFIG LKNLNSMLLF 70 80 90 100 110 120 WDTGMGKTLT AVYIIKHIKE LFPRWIILIF IKKSLYIDPW LNTIRAFAPD ISNNIIFVYY 130 140 150 160 170 180 DSSSSLDKFV NIYRSIENSL NKKNRLLIII DEVHKLISRS VKKDNSERNF TPTYKKLIKL 190 200 210 220 230 240 ANYEGNKILC MSATPIYNNI DEFNNLIGLL RPNVMTFKEE YIINGKLINF KEIRDTLLGL 250 260 270 280 290 300 CSYKRLIEAD SFTETNYVEG YAKKNVIYHN IIMSEEQSKL FNLAEKYDYK TELGGLKTMR 310 320 330 340 350 360 RLVSSFAFYD LKIKGDLNNI EYNDMIKRKL AEFSEFTKTI NFSNNFIEKF KRDDLKNDIL 370 380 390 400 410 420 LEDINNYNIL YQYSCKYIEA CRIILNSRGK VLLFEPLVNF EGIASLKYYF NCFNITYVEY 430 440 450 460 470 480 SSKTLKTRDI EISEYNNYEN NDGKNIKVCI FSYAGSEGIS FKCVNDIIIL DMPWNESELK 490 500 510 520 530 540 QIMGRSIRLN SHNDLPLENR YVNVHFIISY TNNRKSVDKE ILDIIKDKQS KINVVFDLLK 550 560 570 580 590 600 SISIESIHNI HKYIEPVESE TIFDTIRKTR MREMNISNVI VKIKLYPIMY CKDYDRATIL 610 620 630 640 KGLLNKENNI IYNEDTAVAK LTIENNKPVF IIVDDNLIYI ADDYYE

References

[1]

"Mapping of the Heliothis armigera entomopoxvirus (HaEPV) genome, and analysis of genes encoding the HaEPV spheroidin and nucleoside triphosphate phosphohydrolase I proteins." Sriskantha A., Osborne R.J., Dall D.J. J. Gen. Virol. 78:3115-3123(1997) [PubMed: 9400959] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF019224 Genomic DNA. Translation: AAB96624.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR013676. NPHI_C. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF08469. NPHI_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP1_HAEPV

Accession

Primary (citable) accession number: O37319

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)