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Protein

Genome polyprotein

Gene
N/A
Organism
Zucchini yellow mosaic virus (strain Singapore) (ZYMV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Capsid protein: involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.
Nuclear inclusion protein B: an RNA-dependent RNA polymerase that plays an essential role in the virus replication.
Helper component proteinase: required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity (By similarity).By similarity
Cytoplasmic inclusion protein: has helicase activity. It may be involved in replication (By similarity).By similarity
Both 6K peptides are indispensable for virus replication.By similarity
Nuclear inclusion protein A: has RNA-binding and proteolytic activities.

Catalytic activityi

Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei267 – 2671For P1 proteinase activityBy similarity
Active sitei655 – 6551For helper component proteinase activityPROSITE-ProRule annotation
Active sitei728 – 7281For helper component proteinase activityPROSITE-ProRule annotation
Active sitei2090 – 20901For nuclear inclusion protein A activityPROSITE-ProRule annotation
Active sitei2125 – 21251For nuclear inclusion protein A activityPROSITE-ProRule annotation
Active sitei2195 – 21951For nuclear inclusion protein A activityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1252 – 12598ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiC06.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 10 chains:
Alternative name(s):
N-terminal protein
Helper component proteinase (EC:3.4.22.45)
Short name:
HC-pro
6 kDa protein 1
Short name:
6K1
6 kDa protein 2
Short name:
6K2
Alternative name(s):
VPg
Nuclear inclusion protein A (EC:3.4.22.44)
Short name:
NI-a
Short name:
NIa
Alternative name(s):
49 kDa proteinase
Short name:
49 kDa-Pro
NIa-pro
Nuclear inclusion protein B (EC:2.7.7.48)
Short name:
NI-b
Short name:
NIb
Alternative name(s):
RNA-directed RNA polymerase
Capsid protein
Short name:
CP
Alternative name(s):
Coat protein
OrganismiZucchini yellow mosaic virus (strain Singapore) (ZYMV)
Taxonomic identifieri117130 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stagePotyviridaePotyvirus
Virus hostiCitrullus lanatus (Watermelon) (Citrullus vulgaris) [TaxID: 3654]
Cucumis melo (Muskmelon) [TaxID: 3656]
Cucumis sativus (Cucumber) [TaxID: 3659]
Cucurbita pepo (Vegetable marrow) (Summer squash) [TaxID: 3663]
Proteomesi
  • UP000008612 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 30833083Genome polyproteinPRO_0000420037Add
BLAST
Chaini1 – 313313P1 proteinaseSequence analysisPRO_0000040520Add
BLAST
Chaini314 – 769456Helper component proteinaseSequence analysisPRO_0000040521Add
BLAST
Chaini770 – 1115346Protein P3By similarityPRO_0000040522Add
BLAST
Chaini1116 – 1167526 kDa protein 1By similarityPRO_0000040523Add
BLAST
Chaini1168 – 1801634Cytoplasmic inclusion proteinBy similarityPRO_0000040524Add
BLAST
Chaini1802 – 1854536 kDa protein 2By similarityPRO_0000040525Add
BLAST
Chaini1855 – 2044190Viral genome-linked proteinBy similarityPRO_0000040526Add
BLAST
Chaini2045 – 2287243Nuclear inclusion protein ABy similarityPRO_0000040527Add
BLAST
Chaini2288 – 2804517Nuclear inclusion protein BBy similarityPRO_0000040528Add
BLAST
Chaini2805 – 3083279Capsid proteinBy similarityPRO_0000040529Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1918 – 19181O-(5'-phospho-RNA)-tyrosineBy similarity

Post-translational modificationi

VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase (By similarity).By similarity
Potyviral RNA is expressed as two polyproteins which undergo post-translational proteolytic processing. Genome polyprotein is processed by NIa-pro, P1 and HC-pro proteinases resulting in the production of at least ten individual proteins. P3N-PIPO polyprotein is cleaved by P1 and HC-pro proteinases resulting in the production of three individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically. 6K1 is essential for proper proteolytic separation of P3 from CI (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei313 – 3142Cleavage; by P1 proteinaseSequence analysis
Sitei769 – 7702Cleavage; by autolysisPROSITE-ProRule annotation
Sitei1115 – 11162Cleavage; by NIa-proBy similarity
Sitei1167 – 11682Cleavage; by NIa-proBy similarity
Sitei1801 – 18022Cleavage; by NIa-proBy similarity
Sitei2044 – 20452Cleavage; by NIa-proBy similarity
Sitei2287 – 22882Cleavage; by NIa-proBy similarity
Sitei2804 – 28052Cleavage; by NIa-proBy similarity

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliO36979.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini647 – 769123Peptidase C6PROSITE-ProRule annotationAdd
BLAST
Domaini1239 – 1391153Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini1410 – 1569160Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini2045 – 2263219Peptidase C4PROSITE-ProRule annotationAdd
BLAST
Domaini2529 – 2653125RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi365 – 3684Involved in interaction with stylet and aphid transmissionBy similarity
Motifi621 – 6233Involved in virions binding and aphid transmissionBy similarity
Motifi1341 – 13444DECH box
Motifi1894 – 190310Nuclear localization signalSequence analysis

Domaini

The N-terminus of helper component proteinase is involved in interaction with stylets. The central part is involved in interaction with virions and the C-terminus is involved in cell-to cell movement of the virus.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 peptidase C4 domain.PROSITE-ProRule annotation
Contains 1 peptidase C6 domain.PROSITE-ProRule annotationCurated
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011492. DEAD_Flavivir.
IPR001456. HC-pro.
IPR031159. HC_PRO_CPD_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR002540. Pept_S30_P1_potyvir.
IPR009003. Peptidase_S1_PA.
IPR001592. Poty_coat.
IPR001730. Potyv_NIa-pro_dom.
IPR013648. PP_Potyviridae.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
[Graphical view]
PfamiPF07652. Flavi_DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00863. Peptidase_C4. 1 hit.
PF00851. Peptidase_C6. 1 hit.
PF01577. Peptidase_S30. 1 hit.
PF00767. Poty_coat. 1 hit.
PF08440. Poty_PP. 1 hit.
PF00680. RdRP_1. 1 hit.
[Graphical view]
PRINTSiPR00966. NIAPOTYPTASE.
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51744. HC_PRO_CPD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51436. POTYVIRUS_NIA_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Genome polyprotein (identifier: O36979-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAIMIGSIS VPIIGSAQCA TAPIGNRVNI VAPGHMAICK PQMRSHAYYK
60 70 80 90 100
HASQKLSEQS SRGIEVLNSF FNNDPEDAFR LTRNGMSKVK KGPNGRIILR
110 120 130 140 150
KPKARHVFER INLEKSEKEQ KGKFFNGEYD TTVTSIKGVT TSKENDLGAF
160 170 180 190 200
SLRSPFYKRT CKKEKRRITR ENIVCVDDVN NLCERILKIT RDKNIPVEII
210 220 230 240 250
GKRRNHHTLT FKKFKGSFVG KVSLAPERSQ MKHVEMSYGQ FDYILQAICR
260 270 280 290 300
ITSTKHVRDE DIKPGCSGWV FSTDHALTQK YSRLPYLVIR GRDDDGIVNA
310 320 330 340 350
LEPVLFYSDV EHYSFQNEVQ FFNGWRKMFD KLKPHSDHTC KVDHNNEECG
360 370 380 390 400
EMAAVLSQAI FPVLKLSCQV CREKLSRVSF EEFKDFLSRN FMTHESEWST
410 420 430 440 450
LRDGVHCDNV LKLIKGAVQT TQNLKLSSDI MKLVQNHTST HMKQIQDINK
460 470 480 490 500
ALMKGSLVTQ DELDLALKQL LEMTQWFKNH MHLTGEEALK TFRNKRSNKA
510 520 530 540 550
MINPSLLCDN QLDKNGNFIW GERGYHSKRL FKNFFEEVIP SEGYTKYIVR
560 570 580 590 600
NFPNGTRKLA IGSLIVPLNL DRARTALLGE SIEKEPLTSA CISQQNENYI
610 620 630 640 650
HSCCCVTMDD GTPMYSELKS PTKRHLVIGA SGDPKYIDLP ASEAERMYIA
660 670 680 690 700
KEGYCYLNIF LAMLVNVNEN EAKDFTKMIR DVLIPMLGQW PSLMDVATAA
710 720 730 740 750
YILGVFHPET RCAELPRILV DHATQTMHVI DSYGSLTVGY HVLKAGTVNH
760 770 780 790 800
LIQFASNDLQ SEMKHYRVGG TPTQRIRLEE QLIKGIFKPK IMMQLLHDDP
810 820 830 840 850
YILLLGMISP TILVHMYRMR HFERGIEIWI KRDHEIGKIF VILEQLTRKV
860 870 880 890 900
ALAEILVDQL DLISEASPHL LEIMNGCQDN QRAYAPALDL LTIQVEREFS
910 920 930 940 950
NKELKTNGYP DLHQTLHDMR EKMYAKQLHN SWQELSLLEK SCVTVRLKQF
960 970 980 990 1000
SIFTERNLTQ RAKERKHASS LQFVHECFIT TRVHAKSIRD AGVRKLNEAL
1010 1020 1030 1040 1050
VGTCKFFFSC GFRIFARCYS DIIYFVNVCL VFSLVLQMSN TVRNMIAATR
1060 1070 1080 1090 1100
EEKERAMANK ADENERTLMH MYHIFCKKQD DAPIYNDFLE HVRSVRPDLE
1110 1120 1130 1140 1150
ETLLYMAGGE VVTAQAKSAV QIQFEKIIAV LALLTMCFDA ERSDAIFKIL
1160 1170 1180 1190 1200
TKLKIVFGTV GETVRLQGLE DIENLEDDKR LTIDFDINTN EAQSSTTFDV
1210 1220 1230 1240 1250
HFEDWWNRQL QQNRTVPHYR TTGKFLEFTR STAAYVANEI ASSSEGEFLV
1260 1270 1280 1290 1300
RGAVGSGKST SLPAHLAKKG KVLLLEPTRP LAENVSRQLA GDPFFQNVTL
1310 1320 1330 1340 1350
RMRGLSCFGS SNITVMTSGF AFHYYVNNPH QLMEFDFVII DECHVTDSAT
1360 1370 1380 1390 1400
IAFNCALKEY SFAGKLIKVS ATPPGRECDF DTQFAVKVKT EDHLSFNAFV
1410 1420 1430 1440 1450
GAQKTGSNAD MVQHGNNILV YVASYNEVDM LSKLLTERQF SVTKVDGRTM
1460 1470 1480 1490 1500
QLGKTTIETH GTSQKPHFIV ATNIIENGVT LDVECVVDFG LKVVAELDSE
1510 1520 1530 1540 1550
KRCVRYSKKP VSYGERIQRL GRVGRSKPGT ALRIGHTEKG IENIPEFIAT
1560 1570 1580 1590 1600
EAAALSFAYG LSVTTHGVST NNLGKCTVKQ MKCALNFELT PFFTTHLIRH
1610 1620 1630 1640 1650
DGSMHPLIHE ELKQFKLRDS EMVLNKVALP HQFVSQWMDQ SEYERIGVHI
1660 1670 1680 1690 1700
QCHESTRIPF YTNGIPDKVY ERIWKCIQEN KNDALFGKLS SAFPSKVSYT
1710 1720 1730 1740 1750
LSTDPAALPR TIAIIDHLLA EEMMKRNHFD MISSAVTGYS FSLAGIADSF
1760 1770 1780 1790 1800
RKRYMRDHTA HHIAILQQAR AQLLEFNSKN VNINNLSDLE GIGVIKSVVL
1810 1820 1830 1840 1850
QSKQEVSSFL GLRGKWDGRK FANDVILAVM TLFGGGWFMW EYFTKKVNEP
1860 1870 1880 1890 1900
VRVESKKRRS QKLKFRDAYD RKVGREIFGD NDTIGRTFGE AYTKRGKVKG
1910 1920 1930 1940 1950
NNSTKGMGRK TRNFVHLYGV EPEIYSFIRF VDPLTGHTLD ESTHTDISLV
1960 1970 1980 1990 2000
QEEFGNIREK FLENDLISRQ SIINKPGIQA YFMGKGTEEA LKVDLTPHVP
2010 2020 2030 2040 2050
LLLCRNTNAI AGYPERENEL RQTGTPIKVS FKEVPEKNEH VELESKSIYK
2060 2070 2080 2090 2100
GVRDYNGIST IVCQLTNDSD GLKETMYGIG YGPIIITNGH LFRKNNGTLL
2110 2120 2130 2140 2150
VRSWHGEFTV KNTTTLKVHF IEGKDVVLVR MPKDFPPFRS NASFRAPKRE
2160 2170 2180 2190 2200
ERACLVGTNF QEKSLRSTVS ESSMTIPEGT GSYWIHWIST NEGDCGLPMV
2210 2220 2230 2240 2250
STTDGKIIGI HGLASTVSSK NYFVPFTDDL LTTHLSKLDD LTWTQHWLWQ
2260 2270 2280 2290 2300
PSKIAWGSLN LVDEQPGPEF RISNLVKDLL TSGVETQSKR ERWVYESCEG
2310 2320 2330 2340 2350
NLRAVGSAQS ALVTKHVVKG KCPFFEEYLQ THAEANTYFR PLMGEYQPSK
2360 2370 2380 2390 2400
LNKEAFKKDF FKYNKPVVVN QLDHDKFLGA VNGVIRMMCD FEFNECRFIT
2410 2420 2430 2440 2450
DPEEIYDSLN MKAAIGAQYR GKKKEYFEGL DNFDRERLLF QSCERLFNGH
2460 2470 2480 2490 2500
KGLWNGSLKA ELRPLEKVQA NKTRTFTAAP IDTLLGAKVC VDDFNNEFYS
2510 2520 2530 2540 2550
KNLKCPWTVG MTKFYGGWDK LMRELPDGWL YCHADGSQFD SSLTPALLNA
2560 2570 2580 2590 2600
VLIIRSFYME DWWVGQEMLE NLYAEIVYTP ILAPDGTIFK KFRGNNSGQP
2610 2620 2630 2640 2650
STVVDNTLMV VISIYYACMK FGWSYEEIEN KLVFFANGDD LILAVKDEDS
2660 2670 2680 2690 2700
GLLDNMSASF SELGLNYDFS ERTHKREDLW FMSHQAMLVD GMYIPKLEKE
2710 2720 2730 2740 2750
RIVSILEWDR SKEIMHRTEA ICAAMIEAWG HTDLLREIRK FYLWFVEKEE
2760 2770 2780 2790 2800
VRELATLGKA PYIAETALRK LYTDKGAETG ELARYLQALH QDIFFEQGDT
2810 2820 2830 2840 2850
VMLQSDTQTR EAGAGASKKD KDEDKDKKKD VASSSASEKA VATATKDKDV
2860 2870 2880 2890 2900
NAGSHGKIVP RLSKITKKMS LPRVKGSVIL DIDHLLEYKP DQIELYNTRA
2910 2920 2930 2940 2950
SHQQFASWFN QVKAEYDLNE QQMGVVMNGF MVWCIENGTS PDINGVWVMM
2960 2970 2980 2990 3000
DGNEQVEYPL KPIVENAKPT LRQIMHHFSD AAEAYIEMRN AEAPYMPRYG
3010 3020 3030 3040 3050
LLRNLRDRSL ARYAFDFYEV NSKTPDRARE AVAQMKAAAL SNVSSRLFGL
3060 3070 3080
DGNVATTSED TERHTARDVN RNMHTLLGVN TMQ
Note: Produced by conventional translation.
Length:3,083
Mass (Da):351,033
Last modified:December 19, 2001 - v2
Checksum:iB4B6C53C14524A88
GO
Isoform P3N-PIPO polyprotein (identifier: P0CK15-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry P0CK15.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting in P3 ORF.
Length:997
Mass (Da):114,071
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014811 Genomic RNA. Translation: AAB72004.2.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014811 Genomic RNA. Translation: AAB72004.2.

3D structure databases

ProteinModelPortaliO36979.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC06.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011492. DEAD_Flavivir.
IPR001456. HC-pro.
IPR031159. HC_PRO_CPD_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR002540. Pept_S30_P1_potyvir.
IPR009003. Peptidase_S1_PA.
IPR001592. Poty_coat.
IPR001730. Potyv_NIa-pro_dom.
IPR013648. PP_Potyviridae.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
[Graphical view]
PfamiPF07652. Flavi_DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00863. Peptidase_C4. 1 hit.
PF00851. Peptidase_C6. 1 hit.
PF01577. Peptidase_S30. 1 hit.
PF00767. Poty_coat. 1 hit.
PF08440. Poty_PP. 1 hit.
PF00680. RdRP_1. 1 hit.
[Graphical view]
PRINTSiPR00966. NIAPOTYPTASE.
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51744. HC_PRO_CPD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51436. POTYVIRUS_NIA_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Lee K.C., Wong S.M.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: REVIEW.

Entry informationi

Entry nameiPOLG_ZYMVS
AccessioniPrimary (citable) accession number: O36979
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: December 19, 2001
Last modified: October 14, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.