Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ankyrin repeat domain-containing protein A238L

Gene

Mal-047

Organism
African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits activation of the NF-kappa-B transcription factor by preventing binding of NF-kappa-B subunits RELA and NFKB3 to DNA. Inhibits also the activity of the calcium/calmodulin-regulated phosphatase calcineurin (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host NF-kappa-B by virus

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat domain-containing protein A238L
Alternative name(s):
p28
Gene namesi
Ordered Locus Names:Mal-047
ORF Names:5EL
OrganismiAfrican swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV)
Taxonomic identifieri10500 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000000860 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Ankyrin repeat domain-containing protein A238LPRO_0000372838Add
BLAST

Interactioni

Subunit structurei

Interacts with host PPIA and PPP3CA. Interacts with host EP300; this interaction inhibits the association of host AP300 with host RELA, JUN and NFATC2 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-60108N.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi207 – 2115Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4F0ZX-ray1.70C200-239[»]
ProteinModelPortaliO36972.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati48 – 7730ANK 1Add
BLAST
Repeati87 – 11630ANK 2Add
BLAST
Repeati124 – 15330ANK 3Add
BLAST
Repeati158 – 18730ANK 4Add
BLAST

Domaini

The C-terminal region contains a docking motif P-x-I-x-I-T-x-[CS] required and sufficient for binding to calcineurin.By similarity

Sequence similaritiesi

Belongs to the asfivirus A238L family.Curated
Contains 4 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O36972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTIGLFSVE AEHLFVEWVK KCIKKGDLTL FETLFNADPW IVNRCNKNKI
60 70 80 90 100
TVFMLICIYG RLDFLRFLFK QESYPGEIVN HYRRDKDGNS AWHYLAEKNN
110 120 130 140 150
HLLLEEVLDY FGKNGIRVCF PNFNGVTPIM KAAMRGRTLS VLSLLKYGAN
160 170 180 190 200
PNRKDYLKGF TTWDWAVFTG HADLVKTLNK GYQKPLFMHF PLYKLDVFHR
210 220 230
RFKKKPKIII TGCEDNVYEK LPEQNSNFLC VKKLNKYGK
Length:239
Mass (Da):28,080
Last modified:January 1, 1998 - v1
Checksum:iC6FA085308BF2CBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014479 Genomic DNA. Translation: AAB71361.1.
AY261361 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014479 Genomic DNA. Translation: AAB71361.1.
AY261361 Genomic DNA. No translation available.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4F0ZX-ray1.70C200-239[»]
ProteinModelPortaliO36972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60108N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A conserved African swine fever virus IkappaB homolog, 5EL, is nonessential for growth in vitro and virulence in domestic swine."
    Neilan J.G., Lu Z., Kutish G.F., Zsak L., Lewis T.L., Rock D.L.
    Virology 235:377-385(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "African swine fever virus genomes."
    Kutish G.F., Rock D.L.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiVF238_ASFM2
AccessioniPrimary (citable) accession number: O36972
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 1, 1998
Last modified: May 11, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.