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O36966

- POLN_DCVEB

UniProt

O36966 - POLN_DCVEB

Protein

Replicase polyprotein

Gene

ORF1

Organism
Drosophila C virus (strain EB) (DCV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Binds to long dsRNA and to a lesser extent, to siRNA.
    RNA-directed RNA polymerase replicates genomic and antigenomic RNA.

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei97 – 982Cleavage; by ribosomal skipSequence Analysis
    Sitei701 – 7022Cleavage; by 3C-like proteaseSequence Analysis
    Sitei818 – 8192Cleavage; by 3C-like proteaseSequence Analysis
    Sitei901 – 9022Cleavage; by 3C-like proteaseSequence Analysis
    Active sitei1152 – 11521Sequence Analysis
    Sitei1209 – 12102Cleavage; by 3C-like proteaseSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi449 – 4568ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. RNA binding Source: UniProtKB-KW
    4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    5. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: InterPro
    2. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein
    Cleaved into the following 8 chains:
    Gene namesi
    ORF Names:ORF1
    OrganismiDrosophila C virus (strain EB) (DCV)
    Taxonomic identifieri865617 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesDicistroviridaeCripavirus
    Virus hostiDrosophila melanogaster (Fruit fly) [TaxID: 7227]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 901Protein 3BSequence AnalysisPRO_0000399917
    Chaini1 – 17591759Replicase polyproteinPRO_0000399911Add
    BLAST
    Chaini1 – 121121Protein 2ASequence AnalysisPRO_0000399913Add
    BLAST
    Chaini1 – 9797Protein 1ASequence AnalysisPRO_0000399912Add
    BLAST
    Chaini122 – 263142Protein 2BSequence AnalysisPRO_0000399914Add
    BLAST
    Chaini264 – 701438Protein 2CSequence AnalysisPRO_0000399915Add
    BLAST
    Chaini702 – ?Protein 3ASequence AnalysisPRO_0000399916
    Chaini902 – 12093083C-like proteaseSequence AnalysisPRO_0000399918Add
    BLAST
    Chaini1210 – 1759550RNA-directed RNA polymerase 3D-POLSequence AnalysisPRO_0000399919Add
    BLAST

    Post-translational modificationi

    Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond.

    Structurei

    3D structure databases

    ProteinModelPortaliO36966.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 9068DRBMPROSITE-ProRule annotationAdd
    BLAST
    Domaini421 – 595175SF3 helicasePROSITE-ProRule annotationAdd
    BLAST
    Domaini1138 – 117538Peptidase C3Add
    BLAST
    Domaini1483 – 1622140RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili113 – 14028Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation
    Contains 1 peptidase C3 domain.Curated
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
    Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    Gene3Di3.30.160.20. 1 hit.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00035. dsrm. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    PRINTSiPR00918. CALICVIRUSNS.
    SMARTiSM00358. DSRM. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50137. DS_RBD. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O36966-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESDKSMACL NRILMNKMMF VEDKISTLKM VADYYQKEVK YDFDAVESPR     50
    EAPVFRCTCR FLGYTIMTQG IGKKNPKQEA ARQMLLLLSG DVETNPGPVQ 100
    SRPVYYRYND PRYTRLEKAI ERRDDKIKTL IKELRRQIKN RKIYSQGMFD 150
    KLTKQISDGI KDGVGSEQMN GNLTRICDFL ENTLPGLQAN IQATVIDTTD 200
    KYVSLKEDIM KIVLVILLVR LLMVWKKYRA SLCVILIFIF KFYGFDQKLI 250
    DLIMDLKNKI FSQGALEDTV EEVVYHPWFH TCGKIIFAVM AFLTIKKIPG 300
    KQDWDSYITR LDRIPKSIEG AKKITDYCSE YFNIANDQIK MMVLGKTKEE 350
    LQRANGLYGE IQAWAQEVRQ YLELDQRNKI DLDTETANRV EQLWIKGLKF 400
    KSEPLLSKEM SALVHTTLLP AKQLYEYVSC SPVKGGGPRM RPICLWLVGE 450
    SGVGKTEMVY PLCIDVLREM GMIKKDDFHH QVYGRQVETE FWDGYKGQKI 500
    VIYDDAFQKK DDKTAANPEI FEVIRSCNTF PQHLHMAALH DKNTFSAAEL 550
    LLYTTNDYNV KLESITFPDA FFNRMGDMAY KVSPKKEYGI ETEKGNSGKT 600
    YLKLDKSKLD KTKAIDLSVY EFQKIVRDEK SDAGWIDSGS PLDYEDFAKL 650
    VCSKWKEAKQ SSMNKLKFLE EYAIRAQVGS EENSEYGDCI DFVDDIAKRL 700
    QKGETLEEIE FDYASDPEMF TQYYHFKSTI KPASRWQKYK DRMDICLSDC 750
    KTYLAKKYEE IKKILAEHPI LTILGMIGVA LSALAMYYWF SKSLDPVEAE 800
    VAPSGDAKTV RLPRKLVEIG ASGDVKTQKI VKPVVETEWH RNNKGEIEIS 850
    CDECGMHRMS AFNNMTDEEF DNCTYEDLNK DQKRELAQWS TKDSWLGRFF 900
    LSRDRKNKVG IWAEVGQSGD VKTNKAQIKR VEAGAEELVT VALTQGCSDD 950
    AAHNLMIDVF QKNTYRMSYF RGDKRYQLGN CTFVRGWSFI MPYHFVQAVF 1000
    ARRLPPNTII SLSQQMSEDL MQIPLSHFFS AGVDNFYLTD NCVRLPFKNG 1050
    DFRDCVMVNL HSRMCTPHRD LVRHFILTSD QGKLKGSFSG AMATFHVNNM 1100
    GLYRVYNWLN AVRPCDKKIE IFHPEDGFEY PEESYIQRDC YEYNAPTRTG 1150
    DCGSIIGLYN KYLERKIIGM HIAGNDAEEH GYACPLTQEC LETAFSALVN 1200
    KNKKNISSQF YYEIPNMVDP LGDSSVPEGK FYALGKSSIR VGQAVNSSII 1250
    PSRIYGKLSV PTMKPALLKP TILNNKVHNP LLSGLKKCGV DTAVLSDDEV 1300
    LSASQDVCRV MLNQYNKNLN KTKYQRILTY EEAIRGTQDD EFMCAINRTT 1350
    SPGFPYAQMK RNAPGKQQWM GFGEEFDFTS NYALALRKDV EQLIEDCASG 1400
    KISNVIFVDT LKDERRDIAK VNVGKTRVFS AGPQHFVVAF RQYFLPFAAW 1450
    LMHNRISNEV AVGTNVYSSD WERIAKRLKT KGSHVIAGDF GNFDGSLVAQ 1500
    ILWAIFWEIF VVWLKQFIDI ENSEGKRILC ICLGLWSHLV HSVHIYEDNV 1550
    YMWTHSQPSG NPFTVIINCL YNSIIMRLSW IRVMEKFQPR LKSMKWFNEY 1600
    VALITYGDDN VLNIDAKVVE WFNQINISEV MTEMRHEYTD EAKTGDIVKS 1650
    RKLEDIFFLK RKFRFSPELQ RHVAPLKIEV IYEMLNWSRR SIDPDEILMS 1700
    NIETAFREVV YHGKEEYDKL RSAVLALKVP QELPENPQIL TYNQYLHDIE 1750
    YLADPLYDF 1759
    Length:1,759
    Mass (Da):202,942
    Last modified:January 1, 1998 - v1
    Checksum:i49C391F9B0C1AAA9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014388 Genomic RNA. Translation: AAC58807.1.
    PIRiT03725.
    RefSeqiNP_044945.1. NC_001834.1.

    Genome annotation databases

    GeneIDi1449523.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014388 Genomic RNA. Translation: AAC58807.1 .
    PIRi T03725.
    RefSeqi NP_044945.1. NC_001834.1.

    3D structure databases

    ProteinModelPortali O36966.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1449523.

    Family and domain databases

    Gene3Di 3.30.160.20. 1 hit.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00035. dsrm. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    PRINTSi PR00918. CALICVIRUSNS.
    SMARTi SM00358. DSRM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50137. DS_RBD. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The novel genome organization of the insect picorna-like virus Drosophila C virus suggests this virus belongs to a previously undescribed virus family."
      Johnson K.N., Christian P.D.
      J. Gen. Virol. 79:191-203(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster."
      van Rij R.P., Saleh M.C., Berry B., Foo C., Houk A., Antoniewski C., Andino R.
      Genes Dev. 20:2985-2995(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF PROTEIN 1A.
    3. "Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila."
      Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R.
      Nat. Struct. Mol. Biol. 17:547-554(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF PROTEIN 1A.

    Entry informationi

    Entry nameiPOLN_DCVEB
    AccessioniPrimary (citable) accession number: O36966
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3