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O36966

- POLN_DCVEB

UniProt

O36966 - POLN_DCVEB

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Protein

Replicase polyprotein

Gene
ORF1
Organism
Drosophila C virus (strain EB) (DCV)
Status
Reviewed - Annotation score: 4 out of 5 - Protein predictedi

Functioni

Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Binds to long dsRNA and to a lesser extent, to siRNA.2 Publications
RNA-directed RNA polymerase replicates genomic and antigenomic RNA.2 Publications

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei97 – 982Cleavage; by ribosomal skip Reviewed prediction
Sitei701 – 7022Cleavage; by 3C-like protease Reviewed prediction
Sitei818 – 8192Cleavage; by 3C-like protease Reviewed prediction
Sitei901 – 9022Cleavage; by 3C-like protease Reviewed prediction
Active sitei1152 – 11521 Reviewed prediction
Sitei1209 – 12102Cleavage; by 3C-like protease Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi449 – 4568ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. RNA binding Source: UniProtKB-KW
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. RNA helicase activity Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein
Cleaved into the following 8 chains:
Gene namesi
ORF Names:ORF1
OrganismiDrosophila C virus (strain EB) (DCV)
Taxonomic identifieri865617 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesDicistroviridaeCripavirus
Virus hostiDrosophila melanogaster (Fruit fly) [TaxID: 7227]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 901Protein 3B Reviewed predictionPRO_0000399917
Chaini1 – 17591759Replicase polyproteinPRO_0000399911Add
BLAST
Chaini1 – 121121Protein 2A Reviewed predictionPRO_0000399913Add
BLAST
Chaini1 – 9797Protein 1A Reviewed predictionPRO_0000399912Add
BLAST
Chaini122 – 263142Protein 2B Reviewed predictionPRO_0000399914Add
BLAST
Chaini264 – 701438Protein 2C Reviewed predictionPRO_0000399915Add
BLAST
Chaini702 – ?Protein 3A Reviewed predictionPRO_0000399916
Chaini902 – 12093083C-like protease Reviewed predictionPRO_0000399918Add
BLAST
Chaini1210 – 1759550RNA-directed RNA polymerase 3D-POL Reviewed predictionPRO_0000399919Add
BLAST

Post-translational modificationi

Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond.

Structurei

3D structure databases

ProteinModelPortaliO36966.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 9068DRBMAdd
BLAST
Domaini421 – 595175SF3 helicaseAdd
BLAST
Domaini1138 – 117538Peptidase C3Add
BLAST
Domaini1483 – 1622140RdRp catalyticAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili113 – 14028 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00035. dsrm. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00918. CALICVIRUSNS.
SMARTiSM00358. DSRM. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O36966-1 [UniParc]FASTAAdd to Basket

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MESDKSMACL NRILMNKMMF VEDKISTLKM VADYYQKEVK YDFDAVESPR     50
EAPVFRCTCR FLGYTIMTQG IGKKNPKQEA ARQMLLLLSG DVETNPGPVQ 100
SRPVYYRYND PRYTRLEKAI ERRDDKIKTL IKELRRQIKN RKIYSQGMFD 150
KLTKQISDGI KDGVGSEQMN GNLTRICDFL ENTLPGLQAN IQATVIDTTD 200
KYVSLKEDIM KIVLVILLVR LLMVWKKYRA SLCVILIFIF KFYGFDQKLI 250
DLIMDLKNKI FSQGALEDTV EEVVYHPWFH TCGKIIFAVM AFLTIKKIPG 300
KQDWDSYITR LDRIPKSIEG AKKITDYCSE YFNIANDQIK MMVLGKTKEE 350
LQRANGLYGE IQAWAQEVRQ YLELDQRNKI DLDTETANRV EQLWIKGLKF 400
KSEPLLSKEM SALVHTTLLP AKQLYEYVSC SPVKGGGPRM RPICLWLVGE 450
SGVGKTEMVY PLCIDVLREM GMIKKDDFHH QVYGRQVETE FWDGYKGQKI 500
VIYDDAFQKK DDKTAANPEI FEVIRSCNTF PQHLHMAALH DKNTFSAAEL 550
LLYTTNDYNV KLESITFPDA FFNRMGDMAY KVSPKKEYGI ETEKGNSGKT 600
YLKLDKSKLD KTKAIDLSVY EFQKIVRDEK SDAGWIDSGS PLDYEDFAKL 650
VCSKWKEAKQ SSMNKLKFLE EYAIRAQVGS EENSEYGDCI DFVDDIAKRL 700
QKGETLEEIE FDYASDPEMF TQYYHFKSTI KPASRWQKYK DRMDICLSDC 750
KTYLAKKYEE IKKILAEHPI LTILGMIGVA LSALAMYYWF SKSLDPVEAE 800
VAPSGDAKTV RLPRKLVEIG ASGDVKTQKI VKPVVETEWH RNNKGEIEIS 850
CDECGMHRMS AFNNMTDEEF DNCTYEDLNK DQKRELAQWS TKDSWLGRFF 900
LSRDRKNKVG IWAEVGQSGD VKTNKAQIKR VEAGAEELVT VALTQGCSDD 950
AAHNLMIDVF QKNTYRMSYF RGDKRYQLGN CTFVRGWSFI MPYHFVQAVF 1000
ARRLPPNTII SLSQQMSEDL MQIPLSHFFS AGVDNFYLTD NCVRLPFKNG 1050
DFRDCVMVNL HSRMCTPHRD LVRHFILTSD QGKLKGSFSG AMATFHVNNM 1100
GLYRVYNWLN AVRPCDKKIE IFHPEDGFEY PEESYIQRDC YEYNAPTRTG 1150
DCGSIIGLYN KYLERKIIGM HIAGNDAEEH GYACPLTQEC LETAFSALVN 1200
KNKKNISSQF YYEIPNMVDP LGDSSVPEGK FYALGKSSIR VGQAVNSSII 1250
PSRIYGKLSV PTMKPALLKP TILNNKVHNP LLSGLKKCGV DTAVLSDDEV 1300
LSASQDVCRV MLNQYNKNLN KTKYQRILTY EEAIRGTQDD EFMCAINRTT 1350
SPGFPYAQMK RNAPGKQQWM GFGEEFDFTS NYALALRKDV EQLIEDCASG 1400
KISNVIFVDT LKDERRDIAK VNVGKTRVFS AGPQHFVVAF RQYFLPFAAW 1450
LMHNRISNEV AVGTNVYSSD WERIAKRLKT KGSHVIAGDF GNFDGSLVAQ 1500
ILWAIFWEIF VVWLKQFIDI ENSEGKRILC ICLGLWSHLV HSVHIYEDNV 1550
YMWTHSQPSG NPFTVIINCL YNSIIMRLSW IRVMEKFQPR LKSMKWFNEY 1600
VALITYGDDN VLNIDAKVVE WFNQINISEV MTEMRHEYTD EAKTGDIVKS 1650
RKLEDIFFLK RKFRFSPELQ RHVAPLKIEV IYEMLNWSRR SIDPDEILMS 1700
NIETAFREVV YHGKEEYDKL RSAVLALKVP QELPENPQIL TYNQYLHDIE 1750
YLADPLYDF 1759
Length:1,759
Mass (Da):202,942
Last modified:January 1, 1998 - v1
Checksum:i49C391F9B0C1AAA9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF014388 Genomic RNA. Translation: AAC58807.1.
PIRiT03725.
RefSeqiNP_044945.1. NC_001834.1.

Genome annotation databases

GeneIDi1449523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF014388 Genomic RNA. Translation: AAC58807.1 .
PIRi T03725.
RefSeqi NP_044945.1. NC_001834.1.

3D structure databases

ProteinModelPortali O36966.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1449523.

Family and domain databases

Gene3Di 3.30.160.20. 1 hit.
InterProi IPR014720. dsRNA-bd_dom.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00035. dsrm. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00918. CALICVIRUSNS.
SMARTi SM00358. DSRM. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The novel genome organization of the insect picorna-like virus Drosophila C virus suggests this virus belongs to a previously undescribed virus family."
    Johnson K.N., Christian P.D.
    J. Gen. Virol. 79:191-203(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster."
    van Rij R.P., Saleh M.C., Berry B., Foo C., Houk A., Antoniewski C., Andino R.
    Genes Dev. 20:2985-2995(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF PROTEIN 1A.
  3. "Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila."
    Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R.
    Nat. Struct. Mol. Biol. 17:547-554(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF PROTEIN 1A.

Entry informationi

Entry nameiPOLN_DCVEB
AccessioniPrimary (citable) accession number: O36966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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