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Protein

Replicase polyprotein

Gene

ORF1

Organism
Drosophila C virus (strain EB) (DCV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Binds to long dsRNA and to a lesser extent, to siRNA.
RNA-directed RNA polymerase replicates genomic and antigenomic RNA.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1152Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi449 – 456ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein
Cleaved into the following 8 chains:
Gene namesi
ORF Names:ORF1
OrganismiDrosophila C virus (strain EB) (DCV)
Taxonomic identifieri865617 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stagePicornaviralesDicistroviridaeCripavirus
Virus hostiDrosophila melanogaster (Fruit fly) [TaxID: 7227]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000399917? – 901Protein 3BSequence analysis
ChainiPRO_00003999111 – 1759Replicase polyproteinAdd BLAST1759
ChainiPRO_00003999131 – 121Protein 2ASequence analysisAdd BLAST121
ChainiPRO_00003999121 – 97Protein 1ASequence analysisAdd BLAST97
ChainiPRO_0000399914122 – 263Protein 2BSequence analysisAdd BLAST142
ChainiPRO_0000399915264 – 701Protein 2CSequence analysisAdd BLAST438
ChainiPRO_0000399916702 – ?Protein 3ASequence analysis
ChainiPRO_0000399918902 – 12093C-like proteaseSequence analysisAdd BLAST308
ChainiPRO_00003999191210 – 1759RNA-directed RNA polymerase 3D-POLSequence analysisAdd BLAST550

Post-translational modificationi

Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei97 – 98Cleavage; by ribosomal skipSequence analysis2
Sitei701 – 702Cleavage; by 3C-like proteaseSequence analysis2
Sitei818 – 819Cleavage; by 3C-like proteaseSequence analysis2
Sitei901 – 902Cleavage; by 3C-like proteaseSequence analysis2
Sitei1209 – 1210Cleavage; by 3C-like proteaseSequence analysis2

Structurei

3D structure databases

ProteinModelPortaliO36966.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 90DRBMPROSITE-ProRule annotationAdd BLAST68
Domaini421 – 595SF3 helicasePROSITE-ProRule annotationAdd BLAST175
Domaini1138 – 1175Peptidase C3Add BLAST38
Domaini1483 – 1622RdRp catalyticPROSITE-ProRule annotationAdd BLAST140

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili113 – 140Sequence analysisAdd BLAST28

Sequence similaritiesi

Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation
Contains 1 peptidase C3 domain.Curated
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR014720. dsRBD_dom.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR024387. Pept_C3G_Picornavir.
IPR009003. Peptidase_S1_PA.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
[Graphical view]
PfamiPF12381. Peptidase_C3G. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00918. CALICVIRUSNS.
SMARTiSM00358. DSRM. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O36966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESDKSMACL NRILMNKMMF VEDKISTLKM VADYYQKEVK YDFDAVESPR
60 70 80 90 100
EAPVFRCTCR FLGYTIMTQG IGKKNPKQEA ARQMLLLLSG DVETNPGPVQ
110 120 130 140 150
SRPVYYRYND PRYTRLEKAI ERRDDKIKTL IKELRRQIKN RKIYSQGMFD
160 170 180 190 200
KLTKQISDGI KDGVGSEQMN GNLTRICDFL ENTLPGLQAN IQATVIDTTD
210 220 230 240 250
KYVSLKEDIM KIVLVILLVR LLMVWKKYRA SLCVILIFIF KFYGFDQKLI
260 270 280 290 300
DLIMDLKNKI FSQGALEDTV EEVVYHPWFH TCGKIIFAVM AFLTIKKIPG
310 320 330 340 350
KQDWDSYITR LDRIPKSIEG AKKITDYCSE YFNIANDQIK MMVLGKTKEE
360 370 380 390 400
LQRANGLYGE IQAWAQEVRQ YLELDQRNKI DLDTETANRV EQLWIKGLKF
410 420 430 440 450
KSEPLLSKEM SALVHTTLLP AKQLYEYVSC SPVKGGGPRM RPICLWLVGE
460 470 480 490 500
SGVGKTEMVY PLCIDVLREM GMIKKDDFHH QVYGRQVETE FWDGYKGQKI
510 520 530 540 550
VIYDDAFQKK DDKTAANPEI FEVIRSCNTF PQHLHMAALH DKNTFSAAEL
560 570 580 590 600
LLYTTNDYNV KLESITFPDA FFNRMGDMAY KVSPKKEYGI ETEKGNSGKT
610 620 630 640 650
YLKLDKSKLD KTKAIDLSVY EFQKIVRDEK SDAGWIDSGS PLDYEDFAKL
660 670 680 690 700
VCSKWKEAKQ SSMNKLKFLE EYAIRAQVGS EENSEYGDCI DFVDDIAKRL
710 720 730 740 750
QKGETLEEIE FDYASDPEMF TQYYHFKSTI KPASRWQKYK DRMDICLSDC
760 770 780 790 800
KTYLAKKYEE IKKILAEHPI LTILGMIGVA LSALAMYYWF SKSLDPVEAE
810 820 830 840 850
VAPSGDAKTV RLPRKLVEIG ASGDVKTQKI VKPVVETEWH RNNKGEIEIS
860 870 880 890 900
CDECGMHRMS AFNNMTDEEF DNCTYEDLNK DQKRELAQWS TKDSWLGRFF
910 920 930 940 950
LSRDRKNKVG IWAEVGQSGD VKTNKAQIKR VEAGAEELVT VALTQGCSDD
960 970 980 990 1000
AAHNLMIDVF QKNTYRMSYF RGDKRYQLGN CTFVRGWSFI MPYHFVQAVF
1010 1020 1030 1040 1050
ARRLPPNTII SLSQQMSEDL MQIPLSHFFS AGVDNFYLTD NCVRLPFKNG
1060 1070 1080 1090 1100
DFRDCVMVNL HSRMCTPHRD LVRHFILTSD QGKLKGSFSG AMATFHVNNM
1110 1120 1130 1140 1150
GLYRVYNWLN AVRPCDKKIE IFHPEDGFEY PEESYIQRDC YEYNAPTRTG
1160 1170 1180 1190 1200
DCGSIIGLYN KYLERKIIGM HIAGNDAEEH GYACPLTQEC LETAFSALVN
1210 1220 1230 1240 1250
KNKKNISSQF YYEIPNMVDP LGDSSVPEGK FYALGKSSIR VGQAVNSSII
1260 1270 1280 1290 1300
PSRIYGKLSV PTMKPALLKP TILNNKVHNP LLSGLKKCGV DTAVLSDDEV
1310 1320 1330 1340 1350
LSASQDVCRV MLNQYNKNLN KTKYQRILTY EEAIRGTQDD EFMCAINRTT
1360 1370 1380 1390 1400
SPGFPYAQMK RNAPGKQQWM GFGEEFDFTS NYALALRKDV EQLIEDCASG
1410 1420 1430 1440 1450
KISNVIFVDT LKDERRDIAK VNVGKTRVFS AGPQHFVVAF RQYFLPFAAW
1460 1470 1480 1490 1500
LMHNRISNEV AVGTNVYSSD WERIAKRLKT KGSHVIAGDF GNFDGSLVAQ
1510 1520 1530 1540 1550
ILWAIFWEIF VVWLKQFIDI ENSEGKRILC ICLGLWSHLV HSVHIYEDNV
1560 1570 1580 1590 1600
YMWTHSQPSG NPFTVIINCL YNSIIMRLSW IRVMEKFQPR LKSMKWFNEY
1610 1620 1630 1640 1650
VALITYGDDN VLNIDAKVVE WFNQINISEV MTEMRHEYTD EAKTGDIVKS
1660 1670 1680 1690 1700
RKLEDIFFLK RKFRFSPELQ RHVAPLKIEV IYEMLNWSRR SIDPDEILMS
1710 1720 1730 1740 1750
NIETAFREVV YHGKEEYDKL RSAVLALKVP QELPENPQIL TYNQYLHDIE

YLADPLYDF
Length:1,759
Mass (Da):202,942
Last modified:January 1, 1998 - v1
Checksum:i49C391F9B0C1AAA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014388 Genomic RNA. Translation: AAC58807.1.
PIRiT03725.
RefSeqiNP_044945.1. NC_001834.1.

Genome annotation databases

GeneIDi1449523.
KEGGivg:1449523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014388 Genomic RNA. Translation: AAC58807.1.
PIRiT03725.
RefSeqiNP_044945.1. NC_001834.1.

3D structure databases

ProteinModelPortaliO36966.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1449523.
KEGGivg:1449523.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR014720. dsRBD_dom.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR024387. Pept_C3G_Picornavir.
IPR009003. Peptidase_S1_PA.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
[Graphical view]
PfamiPF12381. Peptidase_C3G. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00918. CALICVIRUSNS.
SMARTiSM00358. DSRM. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPOLN_DCVEB
AccessioniPrimary (citable) accession number: O36966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.