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O36966 (POLN_DCVEB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein

Cleaved into the following 8 chains:

  1. Protein 1A
  2. Protein 2A
  3. Protein 2B
  4. Protein 2C
  5. Protein 3A
  6. Protein 3B
  7. 3C-like protease
    EC=3.4.22.-
  8. RNA-directed RNA polymerase 3D-POL
    EC=2.7.7.48
Gene names
ORF Names:ORF1
OrganismDrosophila C virus (strain EB) (DCV)
Taxonomic identifier865617 [NCBI]
Taxonomic lineageViruses › ssRNA positive-strand viruses, no DNA stage › Picornavirales › Dicistroviridae › Cripavirus ›
Virus hostDrosophila melanogaster (Fruit fly) [TaxID: 7227]

Protein attributes

Sequence length1759 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existencePredicted

General annotation (Comments)

Function

Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Binds to long dsRNA and to a lesser extent, to siRNA. Ref.2 Ref.3

RNA-directed RNA polymerase replicates genomic and antigenomic RNA. Ref.2 Ref.3

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Post-translational modification

Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond.

Sequence similarities

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 peptidase C3 domain.

Contains 1 RdRp catalytic domain.

Contains 1 SF3 helicase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17591759Replicase polyprotein
PRO_0000399911
Chain1 – 121121Protein 2A Potential
PRO_0000399913
Chain1 – 9797Protein 1A Potential
PRO_0000399912
Chain122 – 263142Protein 2B Potential
PRO_0000399914
Chain264 – 701438Protein 2C Potential
PRO_0000399915
Chain702 – ?Protein 3A PotentialPRO_0000399916
Chain? – 901Protein 3B PotentialPRO_0000399917
Chain902 – 12093083C-like protease Potential
PRO_0000399918
Chain1210 – 1759550RNA-directed RNA polymerase 3D-POL Potential
PRO_0000399919

Regions

Domain23 – 9068DRBM
Domain421 – 595175SF3 helicase
Domain1138 – 117538Peptidase C3
Domain1483 – 1622140RdRp catalytic
Nucleotide binding449 – 4568ATP Potential
Coiled coil113 – 14028 Potential

Sites

Active site11521 Potential
Site97 – 982Cleavage; by ribosomal skip Potential
Site701 – 7022Cleavage; by 3C-like protease Potential
Site818 – 8192Cleavage; by 3C-like protease Potential
Site901 – 9022Cleavage; by 3C-like protease Potential
Site1209 – 12102Cleavage; by 3C-like protease Potential

Sequences

Sequence LengthMass (Da)Tools
O36966 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 49C391F9B0C1AAA9

FASTA1,759202,942
        10         20         30         40         50         60 
MESDKSMACL NRILMNKMMF VEDKISTLKM VADYYQKEVK YDFDAVESPR EAPVFRCTCR 

        70         80         90        100        110        120 
FLGYTIMTQG IGKKNPKQEA ARQMLLLLSG DVETNPGPVQ SRPVYYRYND PRYTRLEKAI 

       130        140        150        160        170        180 
ERRDDKIKTL IKELRRQIKN RKIYSQGMFD KLTKQISDGI KDGVGSEQMN GNLTRICDFL 

       190        200        210        220        230        240 
ENTLPGLQAN IQATVIDTTD KYVSLKEDIM KIVLVILLVR LLMVWKKYRA SLCVILIFIF 

       250        260        270        280        290        300 
KFYGFDQKLI DLIMDLKNKI FSQGALEDTV EEVVYHPWFH TCGKIIFAVM AFLTIKKIPG 

       310        320        330        340        350        360 
KQDWDSYITR LDRIPKSIEG AKKITDYCSE YFNIANDQIK MMVLGKTKEE LQRANGLYGE 

       370        380        390        400        410        420 
IQAWAQEVRQ YLELDQRNKI DLDTETANRV EQLWIKGLKF KSEPLLSKEM SALVHTTLLP 

       430        440        450        460        470        480 
AKQLYEYVSC SPVKGGGPRM RPICLWLVGE SGVGKTEMVY PLCIDVLREM GMIKKDDFHH 

       490        500        510        520        530        540 
QVYGRQVETE FWDGYKGQKI VIYDDAFQKK DDKTAANPEI FEVIRSCNTF PQHLHMAALH 

       550        560        570        580        590        600 
DKNTFSAAEL LLYTTNDYNV KLESITFPDA FFNRMGDMAY KVSPKKEYGI ETEKGNSGKT 

       610        620        630        640        650        660 
YLKLDKSKLD KTKAIDLSVY EFQKIVRDEK SDAGWIDSGS PLDYEDFAKL VCSKWKEAKQ 

       670        680        690        700        710        720 
SSMNKLKFLE EYAIRAQVGS EENSEYGDCI DFVDDIAKRL QKGETLEEIE FDYASDPEMF 

       730        740        750        760        770        780 
TQYYHFKSTI KPASRWQKYK DRMDICLSDC KTYLAKKYEE IKKILAEHPI LTILGMIGVA 

       790        800        810        820        830        840 
LSALAMYYWF SKSLDPVEAE VAPSGDAKTV RLPRKLVEIG ASGDVKTQKI VKPVVETEWH 

       850        860        870        880        890        900 
RNNKGEIEIS CDECGMHRMS AFNNMTDEEF DNCTYEDLNK DQKRELAQWS TKDSWLGRFF 

       910        920        930        940        950        960 
LSRDRKNKVG IWAEVGQSGD VKTNKAQIKR VEAGAEELVT VALTQGCSDD AAHNLMIDVF 

       970        980        990       1000       1010       1020 
QKNTYRMSYF RGDKRYQLGN CTFVRGWSFI MPYHFVQAVF ARRLPPNTII SLSQQMSEDL 

      1030       1040       1050       1060       1070       1080 
MQIPLSHFFS AGVDNFYLTD NCVRLPFKNG DFRDCVMVNL HSRMCTPHRD LVRHFILTSD 

      1090       1100       1110       1120       1130       1140 
QGKLKGSFSG AMATFHVNNM GLYRVYNWLN AVRPCDKKIE IFHPEDGFEY PEESYIQRDC 

      1150       1160       1170       1180       1190       1200 
YEYNAPTRTG DCGSIIGLYN KYLERKIIGM HIAGNDAEEH GYACPLTQEC LETAFSALVN 

      1210       1220       1230       1240       1250       1260 
KNKKNISSQF YYEIPNMVDP LGDSSVPEGK FYALGKSSIR VGQAVNSSII PSRIYGKLSV 

      1270       1280       1290       1300       1310       1320 
PTMKPALLKP TILNNKVHNP LLSGLKKCGV DTAVLSDDEV LSASQDVCRV MLNQYNKNLN 

      1330       1340       1350       1360       1370       1380 
KTKYQRILTY EEAIRGTQDD EFMCAINRTT SPGFPYAQMK RNAPGKQQWM GFGEEFDFTS 

      1390       1400       1410       1420       1430       1440 
NYALALRKDV EQLIEDCASG KISNVIFVDT LKDERRDIAK VNVGKTRVFS AGPQHFVVAF 

      1450       1460       1470       1480       1490       1500 
RQYFLPFAAW LMHNRISNEV AVGTNVYSSD WERIAKRLKT KGSHVIAGDF GNFDGSLVAQ 

      1510       1520       1530       1540       1550       1560 
ILWAIFWEIF VVWLKQFIDI ENSEGKRILC ICLGLWSHLV HSVHIYEDNV YMWTHSQPSG 

      1570       1580       1590       1600       1610       1620 
NPFTVIINCL YNSIIMRLSW IRVMEKFQPR LKSMKWFNEY VALITYGDDN VLNIDAKVVE 

      1630       1640       1650       1660       1670       1680 
WFNQINISEV MTEMRHEYTD EAKTGDIVKS RKLEDIFFLK RKFRFSPELQ RHVAPLKIEV 

      1690       1700       1710       1720       1730       1740 
IYEMLNWSRR SIDPDEILMS NIETAFREVV YHGKEEYDKL RSAVLALKVP QELPENPQIL 

      1750 
TYNQYLHDIE YLADPLYDF

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References

[1]"The novel genome organization of the insect picorna-like virus Drosophila C virus suggests this virus belongs to a previously undescribed virus family."
Johnson K.N., Christian P.D.
J. Gen. Virol. 79:191-203(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster."
van Rij R.P., Saleh M.C., Berry B., Foo C., Houk A., Antoniewski C., Andino R.
Genes Dev. 20:2985-2995(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF PROTEIN 1A.
[3]"Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila."
Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R.
Nat. Struct. Mol. Biol. 17:547-554(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF PROTEIN 1A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF014388 Genomic RNA. Translation: AAC58807.1.
PIRT03725.
RefSeqNP_044945.1. NC_001834.1.

3D structure databases

ProteinModelPortalO36966.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1449523.

Family and domain databases

Gene3D3.30.160.20. 1 hit.
InterProIPR001159. Ds-RNA-bd.
IPR014720. dsRNA-bd-like_dom.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00035. dsrm. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSPR00918. CALICVIRUSNS.
SMARTSM00358. DSRM. 1 hit.
[Graphical view]
SUPFAMSSF50494. Pept_Ser_Cys. 1 hit.
PROSITEPS50137. DS_RBD. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePOLN_DCVEB
AccessionPrimary (citable) accession number: O36966
Entry history
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: January 1, 1998
Last modified: May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents