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O36966

- POLN_DCVEB

UniProt

O36966 - POLN_DCVEB

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Protein

Replicase polyprotein

Gene

ORF1

Organism
Drosophila C virus (strain EB) (DCV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Binds to long dsRNA and to a lesser extent, to siRNA.
RNA-directed RNA polymerase replicates genomic and antigenomic RNA.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei97 – 982Cleavage; by ribosomal skipSequence Analysis
Sitei701 – 7022Cleavage; by 3C-like proteaseSequence Analysis
Sitei818 – 8192Cleavage; by 3C-like proteaseSequence Analysis
Sitei901 – 9022Cleavage; by 3C-like proteaseSequence Analysis
Active sitei1152 – 11521Sequence Analysis
Sitei1209 – 12102Cleavage; by 3C-like proteaseSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi449 – 4568ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. RNA binding Source: UniProtKB-KW
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. RNA helicase activity Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein
Cleaved into the following 8 chains:
Gene namesi
ORF Names:ORF1
OrganismiDrosophila C virus (strain EB) (DCV)
Taxonomic identifieri865617 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesDicistroviridaeCripavirus
Virus hostiDrosophila melanogaster (Fruit fly) [TaxID: 7227]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 901Protein 3BSequence AnalysisPRO_0000399917
Chaini1 – 17591759Replicase polyproteinPRO_0000399911Add
BLAST
Chaini1 – 121121Protein 2ASequence AnalysisPRO_0000399913Add
BLAST
Chaini1 – 9797Protein 1ASequence AnalysisPRO_0000399912Add
BLAST
Chaini122 – 263142Protein 2BSequence AnalysisPRO_0000399914Add
BLAST
Chaini264 – 701438Protein 2CSequence AnalysisPRO_0000399915Add
BLAST
Chaini702 – ?Protein 3ASequence AnalysisPRO_0000399916
Chaini902 – 12093083C-like proteaseSequence AnalysisPRO_0000399918Add
BLAST
Chaini1210 – 1759550RNA-directed RNA polymerase 3D-POLSequence AnalysisPRO_0000399919Add
BLAST

Post-translational modificationi

Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond.

Structurei

3D structure databases

ProteinModelPortaliO36966.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 9068DRBMPROSITE-ProRule annotationAdd
BLAST
Domaini421 – 595175SF3 helicasePROSITE-ProRule annotationAdd
BLAST
Domaini1138 – 117538Peptidase C3Add
BLAST
Domaini1483 – 1622140RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili113 – 14028Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation
Contains 1 peptidase C3 domain.Curated
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00035. dsrm. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00918. CALICVIRUSNS.
SMARTiSM00358. DSRM. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O36966-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MESDKSMACL NRILMNKMMF VEDKISTLKM VADYYQKEVK YDFDAVESPR
60 70 80 90 100
EAPVFRCTCR FLGYTIMTQG IGKKNPKQEA ARQMLLLLSG DVETNPGPVQ
110 120 130 140 150
SRPVYYRYND PRYTRLEKAI ERRDDKIKTL IKELRRQIKN RKIYSQGMFD
160 170 180 190 200
KLTKQISDGI KDGVGSEQMN GNLTRICDFL ENTLPGLQAN IQATVIDTTD
210 220 230 240 250
KYVSLKEDIM KIVLVILLVR LLMVWKKYRA SLCVILIFIF KFYGFDQKLI
260 270 280 290 300
DLIMDLKNKI FSQGALEDTV EEVVYHPWFH TCGKIIFAVM AFLTIKKIPG
310 320 330 340 350
KQDWDSYITR LDRIPKSIEG AKKITDYCSE YFNIANDQIK MMVLGKTKEE
360 370 380 390 400
LQRANGLYGE IQAWAQEVRQ YLELDQRNKI DLDTETANRV EQLWIKGLKF
410 420 430 440 450
KSEPLLSKEM SALVHTTLLP AKQLYEYVSC SPVKGGGPRM RPICLWLVGE
460 470 480 490 500
SGVGKTEMVY PLCIDVLREM GMIKKDDFHH QVYGRQVETE FWDGYKGQKI
510 520 530 540 550
VIYDDAFQKK DDKTAANPEI FEVIRSCNTF PQHLHMAALH DKNTFSAAEL
560 570 580 590 600
LLYTTNDYNV KLESITFPDA FFNRMGDMAY KVSPKKEYGI ETEKGNSGKT
610 620 630 640 650
YLKLDKSKLD KTKAIDLSVY EFQKIVRDEK SDAGWIDSGS PLDYEDFAKL
660 670 680 690 700
VCSKWKEAKQ SSMNKLKFLE EYAIRAQVGS EENSEYGDCI DFVDDIAKRL
710 720 730 740 750
QKGETLEEIE FDYASDPEMF TQYYHFKSTI KPASRWQKYK DRMDICLSDC
760 770 780 790 800
KTYLAKKYEE IKKILAEHPI LTILGMIGVA LSALAMYYWF SKSLDPVEAE
810 820 830 840 850
VAPSGDAKTV RLPRKLVEIG ASGDVKTQKI VKPVVETEWH RNNKGEIEIS
860 870 880 890 900
CDECGMHRMS AFNNMTDEEF DNCTYEDLNK DQKRELAQWS TKDSWLGRFF
910 920 930 940 950
LSRDRKNKVG IWAEVGQSGD VKTNKAQIKR VEAGAEELVT VALTQGCSDD
960 970 980 990 1000
AAHNLMIDVF QKNTYRMSYF RGDKRYQLGN CTFVRGWSFI MPYHFVQAVF
1010 1020 1030 1040 1050
ARRLPPNTII SLSQQMSEDL MQIPLSHFFS AGVDNFYLTD NCVRLPFKNG
1060 1070 1080 1090 1100
DFRDCVMVNL HSRMCTPHRD LVRHFILTSD QGKLKGSFSG AMATFHVNNM
1110 1120 1130 1140 1150
GLYRVYNWLN AVRPCDKKIE IFHPEDGFEY PEESYIQRDC YEYNAPTRTG
1160 1170 1180 1190 1200
DCGSIIGLYN KYLERKIIGM HIAGNDAEEH GYACPLTQEC LETAFSALVN
1210 1220 1230 1240 1250
KNKKNISSQF YYEIPNMVDP LGDSSVPEGK FYALGKSSIR VGQAVNSSII
1260 1270 1280 1290 1300
PSRIYGKLSV PTMKPALLKP TILNNKVHNP LLSGLKKCGV DTAVLSDDEV
1310 1320 1330 1340 1350
LSASQDVCRV MLNQYNKNLN KTKYQRILTY EEAIRGTQDD EFMCAINRTT
1360 1370 1380 1390 1400
SPGFPYAQMK RNAPGKQQWM GFGEEFDFTS NYALALRKDV EQLIEDCASG
1410 1420 1430 1440 1450
KISNVIFVDT LKDERRDIAK VNVGKTRVFS AGPQHFVVAF RQYFLPFAAW
1460 1470 1480 1490 1500
LMHNRISNEV AVGTNVYSSD WERIAKRLKT KGSHVIAGDF GNFDGSLVAQ
1510 1520 1530 1540 1550
ILWAIFWEIF VVWLKQFIDI ENSEGKRILC ICLGLWSHLV HSVHIYEDNV
1560 1570 1580 1590 1600
YMWTHSQPSG NPFTVIINCL YNSIIMRLSW IRVMEKFQPR LKSMKWFNEY
1610 1620 1630 1640 1650
VALITYGDDN VLNIDAKVVE WFNQINISEV MTEMRHEYTD EAKTGDIVKS
1660 1670 1680 1690 1700
RKLEDIFFLK RKFRFSPELQ RHVAPLKIEV IYEMLNWSRR SIDPDEILMS
1710 1720 1730 1740 1750
NIETAFREVV YHGKEEYDKL RSAVLALKVP QELPENPQIL TYNQYLHDIE

YLADPLYDF
Length:1,759
Mass (Da):202,942
Last modified:January 1, 1998 - v1
Checksum:i49C391F9B0C1AAA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014388 Genomic RNA. Translation: AAC58807.1.
PIRiT03725.
RefSeqiNP_044945.1. NC_001834.1.

Genome annotation databases

GeneIDi1449523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014388 Genomic RNA. Translation: AAC58807.1 .
PIRi T03725.
RefSeqi NP_044945.1. NC_001834.1.

3D structure databases

ProteinModelPortali O36966.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1449523.

Family and domain databases

Gene3Di 3.30.160.20. 1 hit.
InterProi IPR014720. dsRNA-bd_dom.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00035. dsrm. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00918. CALICVIRUSNS.
SMARTi SM00358. DSRM. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The novel genome organization of the insect picorna-like virus Drosophila C virus suggests this virus belongs to a previously undescribed virus family."
    Johnson K.N., Christian P.D.
    J. Gen. Virol. 79:191-203(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster."
    van Rij R.P., Saleh M.C., Berry B., Foo C., Houk A., Antoniewski C., Andino R.
    Genes Dev. 20:2985-2995(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF PROTEIN 1A.
  3. "Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila."
    Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R.
    Nat. Struct. Mol. Biol. 17:547-554(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF PROTEIN 1A.

Entry informationi

Entry nameiPOLN_DCVEB
AccessioniPrimary (citable) accession number: O36966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: January 1, 1998
Last modified: October 1, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3