O36635 (L_HRSVB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Human respiratory syncytial virus B (strain B1)

Taxonomic identifier

79692 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Pneumovirinae › Pneumovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	2166 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular protein N concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with protein P By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the paramyxovirus L protein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2166	2166	RNA-directed RNA polymerase L		PRO_0000365786
Regions
	Domain	693 – 877	185	RdRp catalytic By similarity
Natural variations
	Natural variant	822	1	R → K.
	Natural variant	1662	1	N → H.
	Natural variant	1886	1	L → I.
	Natural variant	2030	1	F → L.

Sequences

	Sequence		Length	Mass (Da)
	O36635 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B2D8925C7935EFFD	FASTA	2,166	249,767
	10 20 30 40 50 60 MDPIINGNSA NVYLTDSYLK GVISFSECNA LGSYLFNGPY LKNDYTNLIS RQSPLLEHMN 70 80 90 100 110 120 LKKLTITQSL ISRYHKGELK LEEPTYFQSL LMTYKSMSSS EQIATTNLLK KIIRRAIEIS 130 140 150 160 170 180 DVKVYAILNK LGLKEKDRVK PNNNSGDENS VLTTIIKDDI LSAVESNQSY TNSDKNHSVN 190 200 210 220 230 240 QNITIKTTLL KKLMCSMQHP PSWLIHWFNL YTKLNNILTQ YRSNEVKSHG FILIDNQTLS 250 260 270 280 290 300 GFQFILNQYG CIVYHKGLKK ITTTTYNQFL TWKDISLSRL NVCLITWISN CLNTLNKSLG 310 320 330 340 350 360 LRCGFNNVVL SQLFLYGDCI LKLFHNEGFY IIKEVEGFIM SLILNITEED QFRKRFYNSM 370 380 390 400 410 420 LNNITDAAIK AQKNLLSRVC HTLLDKTVSD NIINGKWIIL LSKFLKLIKL AGDNNLNNLS 430 440 450 460 470 480 ELYFLFRIFG HPMVDERQAM DAVRINCNET KFYLLSSLST LRGAFIYRII KGFVNTYNRW 490 500 510 520 530 540 PTLRNAIVLP LRWLNYYKLN TYPSLLEITE NDLIILSGLR FYREFHLPKK VDLEMIINDK 550 560 570 580 590 600 AISPPKDLIW TSFPRNYMPS HIQNYIEHEK LKFSESDRSR RVLEYYLRDN KFNECDLYNC 610 620 630 640 650 660 VVNQSYLNNS NHVVSLTGKE RELSVGRMFA MQPGMFRQIQ ILAEKMIAEN ILQFFPESLT 670 680 690 700 710 720 RYGDLELQKI LELKAGISNK SNRYNDNYNN YISKCSIITD LSKFNQAFRY ETSCICSDVL 730 740 750 760 770 780 DELHGVQSLF SWLHLTIPLV TIICTYRHAP PFIKDHVVNL NEVDEQSGLY RYHMGGIEGW 790 800 810 820 830 840 CQKLWTIEAI SLLDLISLKG KFSITALING DNQSIDISKP VRLIEGQTHA QADYLLALNS 850 860 870 880 890 900 LKLLYKEYAG IGHKLKGTET YISRDMQFMS KTIQHNGVYY PASIKKVLRV GPWINTILDD 910 920 930 940 950 960 FKVSLESIGS LTQELEYRGE SLLCSLIFRN IWLYNQIALQ LRNHALCNNK LYLDILKVLK 970 980 990 1000 1010 1020 HLKTFFNLDS IDMALSLYMN LPMLFGGGDP NLLYRSFYRR TPDFLTEAIV HSVFVLSYYT 1030 1040 1050 1060 1070 1080 GHDLQDKLQD LPDDRLNKFL TCVITFDKNP NAEFVTLMRD PQALGSERQA KITSEINRLA 1090 1100 1110 1120 1130 1140 VTEVLSIAPN KIFSKSAQHY TTTEIDLNDI MQNIEPTYPH GLRVVYESLP FYKAEKIVNL 1150 1160 1170 1180 1190 1200 ISGTKSITNI LEKTSAIDTT DINRATDMMR KNITLLIRIL PLDCNKDKRE LLSLENLSIT 1210 1220 1230 1240 1250 1260 ELSKYVRERS WSLSNIVGVT SPSIMFTMDI KYTTSTIASG IIIEKYNVNS LTRGERGPTK 1270 1280 1290 1300 1310 1320 PWVGSSTQEK KTMPVYNRQV LTKKQRDQID LLAKLDWVYA SIDNKDEFME ELSTGTLGLS 1330 1340 1350 1360 1370 1380 YEKAKKLFPQ YLSVNYLHRL TVSSRPCEFP ASIPAYRTTN YHFDTSPINH VLTEKYGDED 1390 1400 1410 1420 1430 1440 IDIVFQNCIS FGLSLMSVVE QFTNICPNRI ILIPKLNEIH LMKPPIFTGD VDIIKLKQVI 1450 1460 1470 1480 1490 1500 QKQHMFLPDK ISLTQYVELF LSNKALKSGS NINSNLILVH KMSDYFHNAY ILSTNLAGHW 1510 1520 1530 1540 1550 1560 ILIIQLMKDS KGIFEKDWGE GYITDHMFIN LNVFFNAYKT YLLCFHKGYG KAKLECDMNT 1570 1580 1590 1600 1610 1620 SDLLCVLELI DSSYWKSMSK VFLEQKVIKY IVNQDTSLHR IKGCHSFKLW FLKRLNNAKF 1630 1640 1650 1660 1670 1680 TVCPWVVNID YHPTHMKAIL SYIDLVRMGL INVDKLTIKN KNKFNDEFYT SNLFYISYNF 1690 1700 1710 1720 1730 1740 SDNTHLLTKQ IRIANSELED NYNKLYHPTP ETLENISLIP VKSNNSNKPK FCISGNTESI 1750 1760 1770 1780 1790 1800 MMSTFSNKMH IKSSTVTTRF NYSKQDLYNL FPNVVIDRII DHSGNTAKSN QLYITTSHQT 1810 1820 1830 1840 1850 1860 SLVRNSASLY CMLPWHHVNR FNFVFSSTGC KISIEYILKD LKIKDPSCIA FIGEGAGNLL 1870 1880 1890 1900 1910 1920 LRTVVELHPD IRYIYRSLKD CNDHSLPIEF LRLYNGHINI DYGENLTIPA TDATNNIHWS 1930 1940 1950 1960 1970 1980 YLHIKFAEPI SIFVCDAELP VTANWSKIII EWSKHVRKCK YCSSVNRCIL IAKYHAQDDI 1990 2000 2010 2020 2030 2040 DFKLDNITIL KTYVCLGSKL KGSEVYLVLT IGPANILPVF DVVQNAKLIF SRTKNFIMPK 2050 2060 2070 2080 2090 2100 KTDKESIDAN IKSLIPFLCY PITKKGIKTS LSKLKSVVNG DILSYSIAGR NEVFSNKLIN 2110 2120 2130 2140 2150 2160 HKHMNILKWL DHVLNFRSAE LNYNHLYMIE STYPYLSELL NSLTTNELKK LIKITGSVLY NLPNEQ

References

[1]

"Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro: clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant." Karron R.A., Buonagurio D.A., Georgiu A.F., Whitehead S.S., Adamus J.E., Clements-Mann M.L., Harris D.O., Randolph V.B., Udem S.A., Murphy B.R., Sidhu M.S. Proc. Natl. Acad. Sci. U.S.A. 94:13961-13966(1997) [PubMed: 9391135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF013254 Genomic RNA. Translation: AAB82439.1. AF013255 Genomic RNA. Translation: AAB82445.1.
RefSeq	NP_056866.1. NC_001781.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1489827.
Family and domain databases
InterPro	IPR003006. Ig/MHC_CS. IPR014023. RNA-dir_pol_cat. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PROSITE	PS00290. IG_MHC. 1 hit. PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_HRSVB

Accession

Primary (citable) accession number: O36635
Secondary accession number(s): O36637

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program