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O36414

- DEN_ALHV1

UniProt

O36414 - DEN_ALHV1

Protein

Deneddylase

Gene

64

Organism
Alcelaphine herpesvirus 1 (strain C500) (AIHV-1) (Malignant catarrhal fever virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei34 – 341PROSITE-ProRule annotation
    Active sitei163 – 1631PROSITE-ProRule annotation
    Active sitei165 – 1651PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Alternative name(s):
    Ubiquitin thioesterase
    Gene namesi
    Name:64
    OrganismiAlcelaphine herpesvirus 1 (strain C500) (AIHV-1) (Malignant catarrhal fever virus)
    Taxonomic identifieri654901 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeMacavirus
    Virus hostiConnochaetes taurinus (Blue wildebeest) [TaxID: 9927]
    ProteomesiUP000000941: Genome

    Subcellular locationi

    Virion tegument. Host cytoplasm. Host nucleus By similarity
    Note: Tightly associated with the capsid.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 26062606DeneddylasePRO_0000405760Add
    BLAST

    Proteomic databases

    PRIDEiO36414.

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 225212Peptidase C76PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1703 – 17086Poly-Pro
    Compositional biasi2069 – 20724Poly-Ala
    Compositional biasi2273 – 2397125Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    InterProiIPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O36414-1 [UniParc]FASTAAdd to Basket

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    MAFQAQTDTG LQLLATASSH QGDCKYGPFA GSQCLSVCVY YLASSFMNNA     50
    PVDSREGLDD VLLHGSTLDR LMRTHNFIPM NEFAQLSSVP KVLITHLWSC 100
    AIETSPELYG LLSDECVVCA PFIMSLRKAL EMNYFEIPQY ILYICNGKSG 150
    AIIIKNKTYF IFDPHCTSSR DTAAVYASAS ASSVVAYVGE ASREYTACHL 200
    YFIPAESSDE PVNEYLLANY GIASALKRSG AYVNLKTLQT HAISESTQPI 250
    APPVPAAPSG MQFFPTQASF PSSQSFVATS TPVPTKALKP TPSHQPSIPG 300
    AASLKTALHM ATVKRKRIAI SPLHSNTESD DSETGKKNTP VKITKPNEAD 350
    VGEVETFWLD DDISTKQMLS ETSSSEKNLP ESIFSSSDWD TDSLLSTHES 400
    PPATPSLKSG QVNYTSTFSL DNEELGGSNL QLQDKTYKLD STTFENLPQL 450
    LTSLEEHIEK VSKYPHKNDM PVIVDHSVNK CYREAVALYT IDSLLSHVIE 500
    QGLISKPEHK SQALNILRYI AIWLNKLSIY TNQVQELINT ELYIPYIYQA 550
    LFATKFDGKL EAMLIEKITK CFQTLHGTSL QDMKNLSKMI QASIKNTPYY 600
    DKAVNVQEET ENLKSAVSLP YFITTEEELK KIQALAANLW KVIQDHNNSV 650
    SHEDSEFHRA VNSVKNFLPI PSDIQPLDYT LSDKADYLGD TVQQLVASIT 700
    QECQTISNHM LSSMQSNVSF SADIPDFYSL RGKICTTLNN IQTSKDHLGL 750
    YNDKLQKARQ QLAYLGYEIS SITNSQWSTD YTEPVTPIPE LGEIQSQLKI 800
    FNTNQQNQQM LQNILDEVES MLQAAKSEQT EQGLQKLAML LPSIEAYLEN 850
    AGTLLGPEGN AQFDRLRKEI TDLIGSVDVM LAYVKNISAH TLATDAQTIS 900
    NFPTDAKQQR VFQQALTQKV KDLFTQVNAS LKTDRPPTFT ENDMAALESL 950
    AVMSNDSTLS TAAAFYTKIT LLLKSKPDCT VPLYDIAHLK SQLATANINS 1000
    STKRSLYMLL AQLNKETVSK KGLEQTQAPA PKGPTADTPV TDSKLIQDSQ 1050
    QNDRHQKEKP LKPKKPQAIS LPAVKDTTKH LKPSKPQNLS LPVSTNKAPV 1100
    EESPESSPIE STSPSHSPVS SMESQNGSFS LESSEEELMD TFAVDDHELS 1150
    DTDHHMDVDK TTTSVAMDDK PSTSPEPPSQ SLSAIVQQEE RLGEEQAWKK 1200
    IQIAFKTLDF TDITYSDWVH VTGITQHHDL KVSETFGPTL ESLMSPLLQK 1250
    LKKMTIGAAL SLTTYGQAFK WPSIDWMTPY KNNVLFYLST VRYPSLVDLA 1300
    EQSRAEIDVL LQLRSSDALL KGTAGTYLES DSRNMLDIVS SIEDAASDYK 1350
    LNVHTEVEKW THQVHNIVNT GDSLPPKPDV ILPSHLLNPA FEQTIASLNK 1400
    DFRDYVYTVE KHLLAELSND FQLLKVLISE TENGFQAWEK ETANKLLQLI 1450
    SHSLQNAPPE VRQYTVSHTD PLTLFDKLIH DSEVSSKLTY SEASAALHWV 1500
    ESTCAHIATQ CQYPAVGAKL QAIIALVAKV KPKLESLVSL ENQANNTDDI 1550
    NIIKQAISSL DPKRITGGTS KVQEWTQKVK DLEKLLADTE LEASVVQNIQ 1600
    LLRHMALTAR STNLLANLKQ KTTSLYEKWV KEHKTGPTSP ITGTIKELDL 1650
    YLTFKLKFIE YYETNQACIF STFALPASKI DAKDALTPLT PQSPVFDFNT 1700
    SGPSPPPPIN FQQRLEAMCH LNYAPTQPIW LQVFPTVDNL SMDYIPIKNA 1750
    SPLSLQVIFN NFIETYFVQA PQGPQKDTSQ YRGSTVLPNV LQAHFGTIAT 1800
    HLINSHWNNI LENSTQALQS YTTVHSLHGT AKQNEFMAII VAVHGLLQSL 1850
    STIYLDTPAN SSDIPVVLSF RATLEIILWI WPKIIFYFLK MKSFQSGVSF 1900
    LQIMINRCLI NLTSAFVIQH ITNSLIMAGV PEPNGYLFCP KYWTQLEPQA 1950
    YLWGDEKFLQ LCSNSQEKAR VCFFVCALES INPVVLGQLW RSLKPTFLPD 2000
    VHTPYDFLKV LVEAAYKPAY DCQVVSKARV DETPPYSYGF PGNSVLRVES 2050
    KTTKPQGVTT IPISGFEMAA AAILQKFNPV IYLSTKKPVF SNEFTGEIFV 2100
    VSPLLDCTGN EEPFSSLLTA PLQPVSENTT LASLYTQLEK DIFRSQKLWL 2150
    QQHLSSPNNL SHLKHPIVLL DSEKKLTGAY SLTNNAPPPQ LLNLRFAYDG 2200
    KGLPNWPMEI LQASTLSYSH QQALEDKTKD WIIDIEDLDD SLCIQNMFSA 2250
    YPPEIHSGPS RSDSEDSEDS LSPTSPTPHL PKAEFTPLPD SPKPPQDPKD 2300
    KSTPNPPRPT TFPTPLLPCY PLSEAYATLP KPISIKPSAR VHFVVPPKSN 2350
    LAADQLLRDF ESLSLQDRPE RPKDGPPDIN YLVPPHGSYI NLQPPNPRMA 2400
    VIGTLRAADQ KSVFTPTRPA GVGSRAKWVS RQLPPHDFVE PVENGDPPGP 2450
    PGSEERKYSI RQESYQDPTV SLLLRQPPII EAVRLFPDKS LTAAQMQQAF 2500
    KQKQLHVCEK SHRKRLLSAP PTIAPNHLSI HEILADRASL VAPRPPFIPI 2550
    DENLDIEPLF VQFIMEVSIE EAKNVLITFI KKIRQAVTHN AQLLASSIQR 2600
    LAALYL 2606
    Length:2,606
    Mass (Da):290,017
    Last modified:January 1, 1998 - v1
    Checksum:i0D3604B55D673082
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005370 Genomic DNA. Translation: AAC58111.1.
    PIRiT03159.
    RefSeqiNP_065563.1. NC_002531.1.

    Genome annotation databases

    GeneIDi911802.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005370 Genomic DNA. Translation: AAC58111.1 .
    PIRi T03159.
    RefSeqi NP_065563.1. NC_002531.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O36414.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 911802.

    Family and domain databases

    InterProi IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the alcelaphine herpesvirus 1 genome."
      Ensser A., Pflanz R., Fleckenstein B.
      J. Virol. 71:6517-6525(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiDEN_ALHV1
    AccessioniPrimary (citable) accession number: O36414
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 8, 2011
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3