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O36414

- DEN_ALHV1

UniProt

O36414 - DEN_ALHV1

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Protein

Deneddylase

Gene

64

Organism
Alcelaphine herpesvirus 1 (strain C500) (AIHV-1) (Malignant catarrhal fever virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341PROSITE-ProRule annotation
Active sitei163 – 1631PROSITE-ProRule annotation
Active sitei165 – 1651PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
Name:64
OrganismiAlcelaphine herpesvirus 1 (strain C500) (AIHV-1) (Malignant catarrhal fever virus)
Taxonomic identifieri654901 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeMacavirus
Virus hostiConnochaetes taurinus (Blue wildebeest) [TaxID: 9927]
ProteomesiUP000000941: Genome

Subcellular locationi

Virion tegument. Host cytoplasm. Host nucleus By similarity
Note: Tightly associated with the capsid.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 26062606DeneddylasePRO_0000405760Add
BLAST

Proteomic databases

PRIDEiO36414.

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 225212Peptidase C76PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1703 – 17086Poly-Pro
Compositional biasi2069 – 20724Poly-Ala
Compositional biasi2273 – 2397125Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O36414-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFQAQTDTG LQLLATASSH QGDCKYGPFA GSQCLSVCVY YLASSFMNNA
60 70 80 90 100
PVDSREGLDD VLLHGSTLDR LMRTHNFIPM NEFAQLSSVP KVLITHLWSC
110 120 130 140 150
AIETSPELYG LLSDECVVCA PFIMSLRKAL EMNYFEIPQY ILYICNGKSG
160 170 180 190 200
AIIIKNKTYF IFDPHCTSSR DTAAVYASAS ASSVVAYVGE ASREYTACHL
210 220 230 240 250
YFIPAESSDE PVNEYLLANY GIASALKRSG AYVNLKTLQT HAISESTQPI
260 270 280 290 300
APPVPAAPSG MQFFPTQASF PSSQSFVATS TPVPTKALKP TPSHQPSIPG
310 320 330 340 350
AASLKTALHM ATVKRKRIAI SPLHSNTESD DSETGKKNTP VKITKPNEAD
360 370 380 390 400
VGEVETFWLD DDISTKQMLS ETSSSEKNLP ESIFSSSDWD TDSLLSTHES
410 420 430 440 450
PPATPSLKSG QVNYTSTFSL DNEELGGSNL QLQDKTYKLD STTFENLPQL
460 470 480 490 500
LTSLEEHIEK VSKYPHKNDM PVIVDHSVNK CYREAVALYT IDSLLSHVIE
510 520 530 540 550
QGLISKPEHK SQALNILRYI AIWLNKLSIY TNQVQELINT ELYIPYIYQA
560 570 580 590 600
LFATKFDGKL EAMLIEKITK CFQTLHGTSL QDMKNLSKMI QASIKNTPYY
610 620 630 640 650
DKAVNVQEET ENLKSAVSLP YFITTEEELK KIQALAANLW KVIQDHNNSV
660 670 680 690 700
SHEDSEFHRA VNSVKNFLPI PSDIQPLDYT LSDKADYLGD TVQQLVASIT
710 720 730 740 750
QECQTISNHM LSSMQSNVSF SADIPDFYSL RGKICTTLNN IQTSKDHLGL
760 770 780 790 800
YNDKLQKARQ QLAYLGYEIS SITNSQWSTD YTEPVTPIPE LGEIQSQLKI
810 820 830 840 850
FNTNQQNQQM LQNILDEVES MLQAAKSEQT EQGLQKLAML LPSIEAYLEN
860 870 880 890 900
AGTLLGPEGN AQFDRLRKEI TDLIGSVDVM LAYVKNISAH TLATDAQTIS
910 920 930 940 950
NFPTDAKQQR VFQQALTQKV KDLFTQVNAS LKTDRPPTFT ENDMAALESL
960 970 980 990 1000
AVMSNDSTLS TAAAFYTKIT LLLKSKPDCT VPLYDIAHLK SQLATANINS
1010 1020 1030 1040 1050
STKRSLYMLL AQLNKETVSK KGLEQTQAPA PKGPTADTPV TDSKLIQDSQ
1060 1070 1080 1090 1100
QNDRHQKEKP LKPKKPQAIS LPAVKDTTKH LKPSKPQNLS LPVSTNKAPV
1110 1120 1130 1140 1150
EESPESSPIE STSPSHSPVS SMESQNGSFS LESSEEELMD TFAVDDHELS
1160 1170 1180 1190 1200
DTDHHMDVDK TTTSVAMDDK PSTSPEPPSQ SLSAIVQQEE RLGEEQAWKK
1210 1220 1230 1240 1250
IQIAFKTLDF TDITYSDWVH VTGITQHHDL KVSETFGPTL ESLMSPLLQK
1260 1270 1280 1290 1300
LKKMTIGAAL SLTTYGQAFK WPSIDWMTPY KNNVLFYLST VRYPSLVDLA
1310 1320 1330 1340 1350
EQSRAEIDVL LQLRSSDALL KGTAGTYLES DSRNMLDIVS SIEDAASDYK
1360 1370 1380 1390 1400
LNVHTEVEKW THQVHNIVNT GDSLPPKPDV ILPSHLLNPA FEQTIASLNK
1410 1420 1430 1440 1450
DFRDYVYTVE KHLLAELSND FQLLKVLISE TENGFQAWEK ETANKLLQLI
1460 1470 1480 1490 1500
SHSLQNAPPE VRQYTVSHTD PLTLFDKLIH DSEVSSKLTY SEASAALHWV
1510 1520 1530 1540 1550
ESTCAHIATQ CQYPAVGAKL QAIIALVAKV KPKLESLVSL ENQANNTDDI
1560 1570 1580 1590 1600
NIIKQAISSL DPKRITGGTS KVQEWTQKVK DLEKLLADTE LEASVVQNIQ
1610 1620 1630 1640 1650
LLRHMALTAR STNLLANLKQ KTTSLYEKWV KEHKTGPTSP ITGTIKELDL
1660 1670 1680 1690 1700
YLTFKLKFIE YYETNQACIF STFALPASKI DAKDALTPLT PQSPVFDFNT
1710 1720 1730 1740 1750
SGPSPPPPIN FQQRLEAMCH LNYAPTQPIW LQVFPTVDNL SMDYIPIKNA
1760 1770 1780 1790 1800
SPLSLQVIFN NFIETYFVQA PQGPQKDTSQ YRGSTVLPNV LQAHFGTIAT
1810 1820 1830 1840 1850
HLINSHWNNI LENSTQALQS YTTVHSLHGT AKQNEFMAII VAVHGLLQSL
1860 1870 1880 1890 1900
STIYLDTPAN SSDIPVVLSF RATLEIILWI WPKIIFYFLK MKSFQSGVSF
1910 1920 1930 1940 1950
LQIMINRCLI NLTSAFVIQH ITNSLIMAGV PEPNGYLFCP KYWTQLEPQA
1960 1970 1980 1990 2000
YLWGDEKFLQ LCSNSQEKAR VCFFVCALES INPVVLGQLW RSLKPTFLPD
2010 2020 2030 2040 2050
VHTPYDFLKV LVEAAYKPAY DCQVVSKARV DETPPYSYGF PGNSVLRVES
2060 2070 2080 2090 2100
KTTKPQGVTT IPISGFEMAA AAILQKFNPV IYLSTKKPVF SNEFTGEIFV
2110 2120 2130 2140 2150
VSPLLDCTGN EEPFSSLLTA PLQPVSENTT LASLYTQLEK DIFRSQKLWL
2160 2170 2180 2190 2200
QQHLSSPNNL SHLKHPIVLL DSEKKLTGAY SLTNNAPPPQ LLNLRFAYDG
2210 2220 2230 2240 2250
KGLPNWPMEI LQASTLSYSH QQALEDKTKD WIIDIEDLDD SLCIQNMFSA
2260 2270 2280 2290 2300
YPPEIHSGPS RSDSEDSEDS LSPTSPTPHL PKAEFTPLPD SPKPPQDPKD
2310 2320 2330 2340 2350
KSTPNPPRPT TFPTPLLPCY PLSEAYATLP KPISIKPSAR VHFVVPPKSN
2360 2370 2380 2390 2400
LAADQLLRDF ESLSLQDRPE RPKDGPPDIN YLVPPHGSYI NLQPPNPRMA
2410 2420 2430 2440 2450
VIGTLRAADQ KSVFTPTRPA GVGSRAKWVS RQLPPHDFVE PVENGDPPGP
2460 2470 2480 2490 2500
PGSEERKYSI RQESYQDPTV SLLLRQPPII EAVRLFPDKS LTAAQMQQAF
2510 2520 2530 2540 2550
KQKQLHVCEK SHRKRLLSAP PTIAPNHLSI HEILADRASL VAPRPPFIPI
2560 2570 2580 2590 2600
DENLDIEPLF VQFIMEVSIE EAKNVLITFI KKIRQAVTHN AQLLASSIQR

LAALYL
Length:2,606
Mass (Da):290,017
Last modified:January 1, 1998 - v1
Checksum:i0D3604B55D673082
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005370 Genomic DNA. Translation: AAC58111.1.
PIRiT03159.
RefSeqiNP_065563.1. NC_002531.1.

Genome annotation databases

GeneIDi911802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005370 Genomic DNA. Translation: AAC58111.1 .
PIRi T03159.
RefSeqi NP_065563.1. NC_002531.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O36414.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 911802.

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of the alcelaphine herpesvirus 1 genome."
    Ensser A., Pflanz R., Fleckenstein B.
    J. Virol. 71:6517-6525(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_ALHV1
AccessioniPrimary (citable) accession number: O36414
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3