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O36414 (DEN_ALHV1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

EC=3.4.19.12
EC=3.4.22.-
Alternative name(s):
Ubiquitin thioesterase
Gene names
Name:64
OrganismAlcelaphine herpesvirus 1 (strain C500) (AIHV-1) (Malignant catarrhal fever virus) [Reference proteome]
Taxonomic identifier654901 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeMacavirus
Virus hostConnochaetes taurinus (Blue wildebeest) [TaxID: 9927]

Protein attributes

Sequence length2606 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument. Host cytoplasm. Host nucleus By similarity. Note: Tightly associated with the capsid By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 26062606Deneddylase
PRO_0000405760

Regions

Domain14 – 225212Peptidase C76
Compositional bias1703 – 17086Poly-Pro
Compositional bias2069 – 20724Poly-Ala
Compositional bias2273 – 2397125Pro-rich

Sites

Active site341 By similarity
Active site1631 By similarity
Active site1651 By similarity

Sequences

Sequence LengthMass (Da)Tools
O36414 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 0D3604B55D673082

FASTA2,606290,017
        10         20         30         40         50         60 
MAFQAQTDTG LQLLATASSH QGDCKYGPFA GSQCLSVCVY YLASSFMNNA PVDSREGLDD 

        70         80         90        100        110        120 
VLLHGSTLDR LMRTHNFIPM NEFAQLSSVP KVLITHLWSC AIETSPELYG LLSDECVVCA 

       130        140        150        160        170        180 
PFIMSLRKAL EMNYFEIPQY ILYICNGKSG AIIIKNKTYF IFDPHCTSSR DTAAVYASAS 

       190        200        210        220        230        240 
ASSVVAYVGE ASREYTACHL YFIPAESSDE PVNEYLLANY GIASALKRSG AYVNLKTLQT 

       250        260        270        280        290        300 
HAISESTQPI APPVPAAPSG MQFFPTQASF PSSQSFVATS TPVPTKALKP TPSHQPSIPG 

       310        320        330        340        350        360 
AASLKTALHM ATVKRKRIAI SPLHSNTESD DSETGKKNTP VKITKPNEAD VGEVETFWLD 

       370        380        390        400        410        420 
DDISTKQMLS ETSSSEKNLP ESIFSSSDWD TDSLLSTHES PPATPSLKSG QVNYTSTFSL 

       430        440        450        460        470        480 
DNEELGGSNL QLQDKTYKLD STTFENLPQL LTSLEEHIEK VSKYPHKNDM PVIVDHSVNK 

       490        500        510        520        530        540 
CYREAVALYT IDSLLSHVIE QGLISKPEHK SQALNILRYI AIWLNKLSIY TNQVQELINT 

       550        560        570        580        590        600 
ELYIPYIYQA LFATKFDGKL EAMLIEKITK CFQTLHGTSL QDMKNLSKMI QASIKNTPYY 

       610        620        630        640        650        660 
DKAVNVQEET ENLKSAVSLP YFITTEEELK KIQALAANLW KVIQDHNNSV SHEDSEFHRA 

       670        680        690        700        710        720 
VNSVKNFLPI PSDIQPLDYT LSDKADYLGD TVQQLVASIT QECQTISNHM LSSMQSNVSF 

       730        740        750        760        770        780 
SADIPDFYSL RGKICTTLNN IQTSKDHLGL YNDKLQKARQ QLAYLGYEIS SITNSQWSTD 

       790        800        810        820        830        840 
YTEPVTPIPE LGEIQSQLKI FNTNQQNQQM LQNILDEVES MLQAAKSEQT EQGLQKLAML 

       850        860        870        880        890        900 
LPSIEAYLEN AGTLLGPEGN AQFDRLRKEI TDLIGSVDVM LAYVKNISAH TLATDAQTIS 

       910        920        930        940        950        960 
NFPTDAKQQR VFQQALTQKV KDLFTQVNAS LKTDRPPTFT ENDMAALESL AVMSNDSTLS 

       970        980        990       1000       1010       1020 
TAAAFYTKIT LLLKSKPDCT VPLYDIAHLK SQLATANINS STKRSLYMLL AQLNKETVSK 

      1030       1040       1050       1060       1070       1080 
KGLEQTQAPA PKGPTADTPV TDSKLIQDSQ QNDRHQKEKP LKPKKPQAIS LPAVKDTTKH 

      1090       1100       1110       1120       1130       1140 
LKPSKPQNLS LPVSTNKAPV EESPESSPIE STSPSHSPVS SMESQNGSFS LESSEEELMD 

      1150       1160       1170       1180       1190       1200 
TFAVDDHELS DTDHHMDVDK TTTSVAMDDK PSTSPEPPSQ SLSAIVQQEE RLGEEQAWKK 

      1210       1220       1230       1240       1250       1260 
IQIAFKTLDF TDITYSDWVH VTGITQHHDL KVSETFGPTL ESLMSPLLQK LKKMTIGAAL 

      1270       1280       1290       1300       1310       1320 
SLTTYGQAFK WPSIDWMTPY KNNVLFYLST VRYPSLVDLA EQSRAEIDVL LQLRSSDALL 

      1330       1340       1350       1360       1370       1380 
KGTAGTYLES DSRNMLDIVS SIEDAASDYK LNVHTEVEKW THQVHNIVNT GDSLPPKPDV 

      1390       1400       1410       1420       1430       1440 
ILPSHLLNPA FEQTIASLNK DFRDYVYTVE KHLLAELSND FQLLKVLISE TENGFQAWEK 

      1450       1460       1470       1480       1490       1500 
ETANKLLQLI SHSLQNAPPE VRQYTVSHTD PLTLFDKLIH DSEVSSKLTY SEASAALHWV 

      1510       1520       1530       1540       1550       1560 
ESTCAHIATQ CQYPAVGAKL QAIIALVAKV KPKLESLVSL ENQANNTDDI NIIKQAISSL 

      1570       1580       1590       1600       1610       1620 
DPKRITGGTS KVQEWTQKVK DLEKLLADTE LEASVVQNIQ LLRHMALTAR STNLLANLKQ 

      1630       1640       1650       1660       1670       1680 
KTTSLYEKWV KEHKTGPTSP ITGTIKELDL YLTFKLKFIE YYETNQACIF STFALPASKI 

      1690       1700       1710       1720       1730       1740 
DAKDALTPLT PQSPVFDFNT SGPSPPPPIN FQQRLEAMCH LNYAPTQPIW LQVFPTVDNL 

      1750       1760       1770       1780       1790       1800 
SMDYIPIKNA SPLSLQVIFN NFIETYFVQA PQGPQKDTSQ YRGSTVLPNV LQAHFGTIAT 

      1810       1820       1830       1840       1850       1860 
HLINSHWNNI LENSTQALQS YTTVHSLHGT AKQNEFMAII VAVHGLLQSL STIYLDTPAN 

      1870       1880       1890       1900       1910       1920 
SSDIPVVLSF RATLEIILWI WPKIIFYFLK MKSFQSGVSF LQIMINRCLI NLTSAFVIQH 

      1930       1940       1950       1960       1970       1980 
ITNSLIMAGV PEPNGYLFCP KYWTQLEPQA YLWGDEKFLQ LCSNSQEKAR VCFFVCALES 

      1990       2000       2010       2020       2030       2040 
INPVVLGQLW RSLKPTFLPD VHTPYDFLKV LVEAAYKPAY DCQVVSKARV DETPPYSYGF 

      2050       2060       2070       2080       2090       2100 
PGNSVLRVES KTTKPQGVTT IPISGFEMAA AAILQKFNPV IYLSTKKPVF SNEFTGEIFV 

      2110       2120       2130       2140       2150       2160 
VSPLLDCTGN EEPFSSLLTA PLQPVSENTT LASLYTQLEK DIFRSQKLWL QQHLSSPNNL 

      2170       2180       2190       2200       2210       2220 
SHLKHPIVLL DSEKKLTGAY SLTNNAPPPQ LLNLRFAYDG KGLPNWPMEI LQASTLSYSH 

      2230       2240       2250       2260       2270       2280 
QQALEDKTKD WIIDIEDLDD SLCIQNMFSA YPPEIHSGPS RSDSEDSEDS LSPTSPTPHL 

      2290       2300       2310       2320       2330       2340 
PKAEFTPLPD SPKPPQDPKD KSTPNPPRPT TFPTPLLPCY PLSEAYATLP KPISIKPSAR 

      2350       2360       2370       2380       2390       2400 
VHFVVPPKSN LAADQLLRDF ESLSLQDRPE RPKDGPPDIN YLVPPHGSYI NLQPPNPRMA 

      2410       2420       2430       2440       2450       2460 
VIGTLRAADQ KSVFTPTRPA GVGSRAKWVS RQLPPHDFVE PVENGDPPGP PGSEERKYSI 

      2470       2480       2490       2500       2510       2520 
RQESYQDPTV SLLLRQPPII EAVRLFPDKS LTAAQMQQAF KQKQLHVCEK SHRKRLLSAP 

      2530       2540       2550       2560       2570       2580 
PTIAPNHLSI HEILADRASL VAPRPPFIPI DENLDIEPLF VQFIMEVSIE EAKNVLITFI 

      2590       2600 
KKIRQAVTHN AQLLASSIQR LAALYL 

« Hide

References

[1]"Primary structure of the alcelaphine herpesvirus 1 genome."
Ensser A., Pflanz R., Fleckenstein B.
J. Virol. 71:6517-6525(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF005370 Genomic DNA. Translation: AAC58111.1.
PIRT03159.
RefSeqNP_065563.1. NC_002531.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEO36414.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID911802.

Phylogenomic databases

ProtClustDBCLSP2511248.

Family and domain databases

InterProIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_ALHV1
AccessionPrimary (citable) accession number: O36414
Entry history
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries