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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

61

Organism
Alcelaphine herpesvirus 1 (strain C500) (AlHV-1) (Malignant catarrhal fever virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei177 – 1771SubstrateBy similarity
Sitei193 – 1931Important for hydrogen atom transferBy similarity
Binding sitei223 – 2231Substrate; via amide nitrogenBy similarity
Active sitei393 – 3931Proton acceptorBy similarity
Active sitei395 – 3951Cysteine radical intermediateBy similarity
Active sitei397 – 3971Proton acceptorBy similarity
Sitei409 – 4091Important for hydrogen atom transferBy similarity
Sitei726 – 7261Important for electron transferBy similarity
Sitei727 – 7271Important for electron transferBy similarity
Sitei776 – 7761Interacts with thioredoxin/glutaredoxinBy similarity
Sitei779 – 7791Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Name:61
OrganismiAlcelaphine herpesvirus 1 (strain C500) (AlHV-1) (Malignant catarrhal fever virus)
Taxonomic identifieri654901 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeMacavirus
Virus hostiConnochaetes taurinus (Blue wildebeest) [TaxID: 9927]
ProteomesiUP000000941: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 780780Ribonucleoside-diphosphate reductase large subunitPRO_0000405696Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi193 ↔ 409Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterodimer of a large and a small chain.By similarity

Structurei

3D structure databases

ProteinModelPortaliO36411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni192 – 1932Substrate bindingBy similarity
Regioni393 – 3975Substrate bindingBy similarity
Regioni595 – 5995Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O36411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASAEATPQQ QIDQLKVNAG WDMKSNIKAG LLHHEEMEKK ATKTVTEYIE
60 70 80 90 100
KFADVLEENV LRFLQDNAEM LELCIYMYEQ LPAYKAVKSR GILSAKRFYD
110 120 130 140 150
TYVLRTADGS CYESVSHCFM RIAAFCTVQV LTNSALKITI LYLGRDKLFK
160 170 180 190 200
DLPCSPTMDL FIYFFSPLSH QLVCCATPIM RSAGLRDANL ASCFLINPDL
210 220 230 240 250
STEKSTTTAL LQELTMLLSA KSGVGCNVTS FGVDEKCIQS CVGLINSQVE
260 270 280 290 300
FFNDQNPRPV SVAAYMEVWH SQIQEFLAVK LPENPSRCAS IYQGLCIPKL
310 320 330 340 350
FFEKFIEDPG QNWYLFKPEK SGNLANLYGD EFRSEYERLV GIGCYADAIP
360 370 380 390 400
IKSLMFLIIN TIIKTGSPYI IYKEACNEHH WKNMEGCAIA SANLCAEVIQ
410 420 430 440 450
YPGADVSTCN LANVCLPMCL VTVSNDSHSP LEKTYCGNVI ESQAVSGVGF
460 470 480 490 500
SMPILHAAVE VAVFLVNCAI AGGKCVTPGM ERGQRERSMG IGVHGLADVF
510 520 530 540 550
AEMGYSYLDE RAARLDVEIF ENMYFRAVKT SNNICRLGGG RPFEGWGESK
560 570 580 590 600
LRHGFFHWQG WEDVNLSIPI TEWEKLSRRC ISSGVYNSQF IALMPTVGSS
610 620 630 640 650
LLTGFSESYY PYFANISSKV SSKEEVMKPN MTFWNRVSKE DLDTVRFFSG
660 670 680 690 700
DVALFPEPLK EKYSLFLSAF DYCAEKQLAR ARLRAPFVDQ SQSHSFHLKE
710 720 730 740 750
ENVVSARFLK DLILSGYTLG LKTIMYYCKV KKQSTMSSFQ CLRDQNKSEM
760 770 780
TGNDQGVEPE SYIKCTAAGG ETSEACLHCQ
Length:780
Mass (Da):87,362
Last modified:January 1, 1998 - v1
Checksum:i0B4C197AB8D76DDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005370 Genomic DNA. Translation: AAC58108.1.
PIRiT03156.
RefSeqiNP_065560.1. NC_002531.1.

Genome annotation databases

GeneIDi911785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005370 Genomic DNA. Translation: AAC58108.1.
PIRiT03156.
RefSeqiNP_065560.1. NC_002531.1.

3D structure databases

ProteinModelPortaliO36411.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi911785.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of the alcelaphine herpesvirus 1 genome."
    Ensser A., Pflanz R., Fleckenstein B.
    J. Virol. 71:6517-6525(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRIR1_ALHV1
AccessioniPrimary (citable) accession number: O36411
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: January 1, 1998
Last modified: March 4, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.