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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RIR1

Organism
Alcelaphine herpesvirus 1 (strain C500) (AlHV-1) (Malignant catarrhal fever virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei177SubstrateUniRule annotation1
Sitei193Important for hydrogen atom transferUniRule annotation1
Binding sitei223Substrate; via amide nitrogenUniRule annotation1
Active sitei393Proton acceptorUniRule annotation1
Active sitei395Cysteine radical intermediateUniRule annotation1
Active sitei397Proton acceptorUniRule annotation1
Sitei409Important for hydrogen atom transferUniRule annotation1
Sitei726Important for electron transferUniRule annotation1
Sitei727Important for electron transferUniRule annotation1
Sitei776Interacts with thioredoxin/glutaredoxinUniRule annotation1
Sitei779Interacts with thioredoxin/glutaredoxinUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Short name:
R1UniRule annotation
Alternative name(s):
Ribonucleotide reductase large subunitUniRule annotation
Gene namesi
Name:RIR1UniRule annotation
Ordered Locus Names:61
OrganismiAlcelaphine herpesvirus 1 (strain C500) (AlHV-1) (Malignant catarrhal fever virus)
Taxonomic identifieri654901 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeMacavirus
Virus hostiConnochaetes taurinus (Blue wildebeest) [TaxID: 9927]
Proteomesi
  • UP000000941 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004056961 – 780Ribonucleoside-diphosphate reductase large subunitAdd BLAST780

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi193 ↔ 409Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO36411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni192 – 193Substrate bindingUniRule annotation2
Regioni393 – 397Substrate bindingUniRule annotation5
Regioni595 – 599Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase large chain family.UniRule annotation

Phylogenomic databases

KOiK10807.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O36411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASAEATPQQ QIDQLKVNAG WDMKSNIKAG LLHHEEMEKK ATKTVTEYIE
60 70 80 90 100
KFADVLEENV LRFLQDNAEM LELCIYMYEQ LPAYKAVKSR GILSAKRFYD
110 120 130 140 150
TYVLRTADGS CYESVSHCFM RIAAFCTVQV LTNSALKITI LYLGRDKLFK
160 170 180 190 200
DLPCSPTMDL FIYFFSPLSH QLVCCATPIM RSAGLRDANL ASCFLINPDL
210 220 230 240 250
STEKSTTTAL LQELTMLLSA KSGVGCNVTS FGVDEKCIQS CVGLINSQVE
260 270 280 290 300
FFNDQNPRPV SVAAYMEVWH SQIQEFLAVK LPENPSRCAS IYQGLCIPKL
310 320 330 340 350
FFEKFIEDPG QNWYLFKPEK SGNLANLYGD EFRSEYERLV GIGCYADAIP
360 370 380 390 400
IKSLMFLIIN TIIKTGSPYI IYKEACNEHH WKNMEGCAIA SANLCAEVIQ
410 420 430 440 450
YPGADVSTCN LANVCLPMCL VTVSNDSHSP LEKTYCGNVI ESQAVSGVGF
460 470 480 490 500
SMPILHAAVE VAVFLVNCAI AGGKCVTPGM ERGQRERSMG IGVHGLADVF
510 520 530 540 550
AEMGYSYLDE RAARLDVEIF ENMYFRAVKT SNNICRLGGG RPFEGWGESK
560 570 580 590 600
LRHGFFHWQG WEDVNLSIPI TEWEKLSRRC ISSGVYNSQF IALMPTVGSS
610 620 630 640 650
LLTGFSESYY PYFANISSKV SSKEEVMKPN MTFWNRVSKE DLDTVRFFSG
660 670 680 690 700
DVALFPEPLK EKYSLFLSAF DYCAEKQLAR ARLRAPFVDQ SQSHSFHLKE
710 720 730 740 750
ENVVSARFLK DLILSGYTLG LKTIMYYCKV KKQSTMSSFQ CLRDQNKSEM
760 770 780
TGNDQGVEPE SYIKCTAAGG ETSEACLHCQ
Length:780
Mass (Da):87,362
Last modified:January 1, 1998 - v1
Checksum:i0B4C197AB8D76DDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005370 Genomic DNA. Translation: AAC58108.1.
PIRiT03156.
RefSeqiNP_065560.1. NC_002531.1.

Genome annotation databases

GeneIDi911785.
KEGGivg:911785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005370 Genomic DNA. Translation: AAC58108.1.
PIRiT03156.
RefSeqiNP_065560.1. NC_002531.1.

3D structure databases

ProteinModelPortaliO36411.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi911785.
KEGGivg:911785.

Phylogenomic databases

KOiK10807.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_ALHV1
AccessioniPrimary (citable) accession number: O36411
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.