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Protein

Thymidine kinase

Gene

TK

Organism
Alcelaphine herpesvirus 1 (strain C500) (AlHV-1) (Malignant catarrhal fever virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei272 – 2721Proton acceptorUniRule annotation
Binding sitei310 – 3101SubstrateUniRule annotation
Binding sitei400 – 4001ATPUniRule annotation
Binding sitei406 – 4061SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi245 – 2528ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:TKUniRule annotation
Ordered Locus Names:21
OrganismiAlcelaphine herpesvirus 1 (strain C500) (AlHV-1) (Malignant catarrhal fever virus)
Taxonomic identifieri654901 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeMacavirus
Virus hostiConnochaetes taurinus (Blue wildebeest) [TaxID: 9927]
Proteomesi
  • UP000000941 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Thymidine kinasePRO_0000405714Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO36371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae thymidine kinase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

O36371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNSHNNYN TPRRQNYDVP KAWEEDSLYE NIDFLTQPAV SSDTSEDEEP
60 70 80 90 100
LPLGRETGRG SRGSTRPKTP RGLVRPRQDA DNPSSDEGFD WEPLESFTSQ
110 120 130 140 150
PGAFSFLEPP RLSSSNTASG LRSSCSDFTI LGSSGENPTS HANLSEEEMY
160 170 180 190 200
EEIPPLASNP SDERHPRQLS GAVAQGAIRK SPRKLKFKPA KFKGLSSSLL
210 220 230 240 250
NPFTASDSGR RARRQTPTCQ PGFTPIFQDL GEPHYVKACT VFFEGCMAAG
260 270 280 290 300
KTTLLNFARQ TLSDDEALTI PEPMRFWTEV YTNVLSQIVK INKECKPGKT
310 320 330 340 350
STTAELVSCQ LKFATPLKTQ SLFLQRSVKK DSEMQPVGPL DKWVIVDRHQ
360 370 380 390 400
LSALVVFPLV LMRRGMLSFS DFFNLLGMFE AHPGEVIALM SVNVEENFTR
410 420 430 440 450
LKKRGRVCER HIDRDYIKEI KGSFNAAYCA WLFLQYFSIQ TTMQICMGLS
460 470 480 490 500
SLDEACATEG VCHTTASRIW NNSMLVTLSD IISQFSNDYT VQNVCYNFFS
510 520 530 540 550
QLSTLKFVVI DLSAFRHDVP GAWGEFYMQV MKNGDIKTRV MDFTAIKALA
560
DTAHNTHASL D
Length:561
Mass (Da):62,796
Last modified:January 1, 1998 - v1
Checksum:i5ED9F2955E4669AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005370 Genomic DNA. Translation: AAC58068.1.
PIRiT03116. A60030.
RefSeqiNP_065520.1. NC_002531.1.

Genome annotation databases

GeneIDi911756.
KEGGivg:911756.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005370 Genomic DNA. Translation: AAC58068.1.
PIRiT03116. A60030.
RefSeqiNP_065520.1. NC_002531.1.

3D structure databases

ProteinModelPortaliO36371.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi911756.
KEGGivg:911756.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_ALHV1
AccessioniPrimary (citable) accession number: O36371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.