Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aspartyl aminopeptidase 1

Gene

aap1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Aspartyl aminopeptidase that is able to remove aspartyl residue at N-terminus of angiotensin I. Acts also as a chaperone and efficiently suppressed the thermal aggregation of citrate synthase.1 Publication

Catalytic activityi

Release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi87Zinc 1By similarity1
Binding sitei162SubstrateBy similarity1
Metal bindingi256Zinc 1By similarity1
Metal bindingi256Zinc 2By similarity1
Binding sitei292SubstrateBy similarity1
Metal bindingi293Zinc 2By similarity1
Metal bindingi337Zinc 1By similarity1
Binding sitei337SubstrateBy similarity1
Binding sitei340SubstrateBy similarity1
Binding sitei365SubstrateBy similarity1
Binding sitei372SubstrateBy similarity1
Metal bindingi431Zinc 2By similarity1

GO - Molecular functioni

  • metalloaminopeptidase activity Source: PomBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chaperone-mediated protein folding Source: PomBase
  • proteolysis Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Chaperone, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.11.21. 5613.

Protein family/group databases

MEROPSiM18.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl aminopeptidase 1 (EC:3.4.11.21)
Gene namesi
Name:aap1
ORF Names:SPAC4F10.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4F10.02.
PomBaseiSPAC4F10.02. aap1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001734541 – 467Aspartyl aminopeptidase 1Add BLAST467

Proteomic databases

MaxQBiO36014.
PRIDEiO36014.

Interactioni

Subunit structurei

Tetrahedron-shaped homododecamer built from six homodimers.By similarity

Protein-protein interaction databases

BioGridi280020. 2 interactors.
MINTiMINT-4672902.

Structurei

3D structure databases

ProteinModelPortaliO36014.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M18 family.Curated

Phylogenomic databases

HOGENOMiHOG000253244.
KOiK01267.
OMAiAIHMNRE.
OrthoDBiEOG092C3JCE.
PhylomeDBiO36014.

Family and domain databases

Gene3Di2.30.250.10. 1 hit.
InterProiIPR001948. Peptidase_M18.
IPR023358. Peptidase_M18_dom2.
[Graphical view]
PfamiPF02127. Peptidase_M18. 1 hit.
[Graphical view]
PRINTSiPR00932. AMINO1PTASE.

Sequencei

Sequence statusi: Complete.

O36014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATAKSCAL DFLDFVNASP TPYHAVQNLA EHYMSHGFQY LSEKSDWQSK
60 70 80 90 100
IEPGNSYFVT RNKSSIIAFS IGKKWKPGNG FSIIATHTDS PTLRLKPKSQ
110 120 130 140 150
KSAYGYLQVG VEKYGGGIWH TWFDRDLSLA GRVMVEEEDG RVIQYNVHID
160 170 180 190 200
RPLLRIPTLA IHLDPSANSS FSFNMETEFV PLIGLENELA KEETSDNGDK
210 220 230 240 250
YHHPVLLSLL ANEISKSLET TIDPSKIVDF ELILGDAEKA RLGGIHEEFV
260 270 280 290 300
FSPRLDNLGM TFCASQALTK SLENNSLDNE SCVRVVPSFD HEEIGSVSAQ
310 320 330 340 350
GAESTFLPAV LQRICELGKE SSLFSISMVK SFLVSADMAH AMHPNYSSRY
360 370 380 390 400
ENSNTPFLNK GTVIKVNANQ RYTTNSAGIV LLKKVAQLAD VPIQSFVVRN
410 420 430 440 450
DSPCGSTIGP KLAAMTGMRT LDLGNPMLSM HSCREMCGSK DFEYAVVLFS
460
SFFQNFANLE EKIIIDE
Length:467
Mass (Da):51,741
Last modified:January 1, 1998 - v1
Checksum:i4A162926DBE3D086
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76 – 77KP → MI in BAA13937 (PubMed:9501991).Curated2
Sequence conflicti262 – 263FC → SG in BAA13937 (PubMed:9501991).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11706.1.
D89276 mRNA. Translation: BAA13937.1.
PIRiT38806.
T43206.
RefSeqiNP_594745.1. NM_001020172.2.

Genome annotation databases

EnsemblFungiiSPAC4F10.02.1; SPAC4F10.02.1:pep; SPAC4F10.02.
GeneIDi2543605.
KEGGispo:SPAC4F10.02.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11706.1.
D89276 mRNA. Translation: BAA13937.1.
PIRiT38806.
T43206.
RefSeqiNP_594745.1. NM_001020172.2.

3D structure databases

ProteinModelPortaliO36014.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280020. 2 interactors.
MINTiMINT-4672902.

Protein family/group databases

MEROPSiM18.002.

Proteomic databases

MaxQBiO36014.
PRIDEiO36014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4F10.02.1; SPAC4F10.02.1:pep; SPAC4F10.02.
GeneIDi2543605.
KEGGispo:SPAC4F10.02.

Organism-specific databases

EuPathDBiFungiDB:SPAC4F10.02.
PomBaseiSPAC4F10.02. aap1.

Phylogenomic databases

HOGENOMiHOG000253244.
KOiK01267.
OMAiAIHMNRE.
OrthoDBiEOG092C3JCE.
PhylomeDBiO36014.

Enzyme and pathway databases

BRENDAi3.4.11.21. 5613.

Miscellaneous databases

PROiO36014.

Family and domain databases

Gene3Di2.30.250.10. 1 hit.
InterProiIPR001948. Peptidase_M18.
IPR023358. Peptidase_M18_dom2.
[Graphical view]
PfamiPF02127. Peptidase_M18. 1 hit.
[Graphical view]
PRINTSiPR00932. AMINO1PTASE.
ProtoNetiSearch...

Entry informationi

Entry nameiDNPEP_SCHPO
AccessioniPrimary (citable) accession number: O36014
Secondary accession number(s): P78925
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.